Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Option or wrapper script for automatic .bam split, per cluster, based on the default cell ranger clustering results and output folder structure? #6

Open
vertesy opened this issue Nov 22, 2019 · 0 comments

Comments

@vertesy
Copy link

vertesy commented Nov 22, 2019

First off, thanks for sharing this tool, its really great! I understand that it is "not officially supported", but since you were willing to share it, I thought you might help out with a problem that I am sure you solved already in house.

Does this script have an option, or do you have a wrapper script to automatically split the /outs/possorted_genome_bam.bam based on the clustering results from Cell Ranger /outs/analysis/clustering/kmeans_6_clusters/clusters.csv assuming the default output folder structure of Cell Ranger?

The clustering results are stored as:

image

...while (as I understand) the split-bam requires a simple list of cell barcodes for one cluster, then execute it for multiple clusters.

Thanks!

Abel

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant