diff --git a/README.md b/README.md index 7de0aee..baac250 100644 --- a/README.md +++ b/README.md @@ -1 +1,19 @@ -# 2022-mtx-not-in-mgx-pairs +# Identifying sequences that are in a metatranscriptome but not in a metagenome + +This repository curates a set of paired metagenomes and metatranscriptomes and provides a pipeline to rapidly identify the fraction of sequences in a metatranscriptome that are not in a metagenome. +The pipeline is shaped around a metadata file, 'inputs/metadata-paired-mgx-mtx.tsv', that contains a sample name (`sample_name`), metagenome SRA run accession (`mgx_run_accession`; `SRR*`, `ERR*`, `DRR*`), metatranscriptome SRA run accession (`mtx_run_accession`), and a sample type (`sample_type`). +Using the run accessions, it downloads the sequencing data from the SRA and generates a [FracMinHash sketch](https://www.biorxiv.org/content/10.1101/2022.01.11.475838v2.abstract) of each run. +Then, it uses the paired information encoded in the metadata table to subtract the metagenome sketch from the metatranscriptome sketch. +This produces an estimate of the fraction metatranscriptome sequences not found in the paired metagenome. +These estimates are also clustered by `sample_type` to generate biome-specific estimates. +The pipeline also analyzes the fraction of metatranscriptome-specific sequences that are shared between samples to discover what fraction of sequences we are systematically missing within and across biomes. + +Some metagenome and metatranscripome pairs are true pairs that were extracted from the same sample while others are from separate samples taken from the same location at the same time. + +## Getting started with this repository + +TBD + +## Next steps + +TBD diff --git a/inputs/metadata-paired-mgx-mtx.tsv b/inputs/metadata-paired-mgx-mtx.tsv new file mode 100644 index 0000000..1a633a4 --- /dev/null +++ b/inputs/metadata-paired-mgx-mtx.tsv @@ -0,0 +1,1281 @@ +sample_name mtx_study_accession mtx_run_accession mgx_study_accession mgx_run_accession sample_type +C_2_1 PRJNA406858 SRR6032600 PRJNA406858 SRR6032602 activated_sludge +B_2_1 PRJNA406858 SRR6032604 PRJNA406858 SRR6032601 activated_sludge +E_2_1 PRJNA406858 SRR6032605 PRJNA406858 SRR6032603 activated_sludge +rumen_microbiome_of_beef_cattle_101 PRJNA448333 SRR8416057 PRJNA448333 SRR8397906 cattle_rumen +rumen_microbiome_of_beef_cattle_103 PRJNA448333 SRR8416058 PRJNA448333 SRR8404214 cattle_rumen +rumen_microbiome_of_beef_cattle_104 PRJNA448333 SRR8416055 PRJNA448333 SRR8397905 cattle_rumen +rumen_microbiome_of_beef_cattle_105 PRJNA448333 SRR8416056 PRJNA448333 SRR8397904 cattle_rumen +rumen_microbiome_of_beef_cattle_106 PRJNA448333 SRR8416061 PRJNA448333 SRR8397903 cattle_rumen +rumen_microbiome_of_beef_cattle_107 PRJNA448333 SRR8416062 PRJNA448333 SRR8397910 cattle_rumen +rumen_microbiome_of_beef_cattle_112 PRJNA448333 SRR8416059 PRJNA448333 SRR8397909 cattle_rumen +rumen_microbiome_of_beef_cattle_201 PRJNA448333 SRR8416060 PRJNA448333 SRR8397908 cattle_rumen +rumen_microbiome_of_beef_cattle_202 PRJNA448333 SRR8416064 PRJNA448333 SRR8397907 cattle_rumen +rumen_microbiome_of_beef_cattle_203 PRJNA448333 SRR8416065 PRJNA448333 SRR8397902 cattle_rumen +rumen_microbiome_of_beef_cattle_205 PRJNA448333 SRR8416069 PRJNA448333 SRR8397901 cattle_rumen +rumen_microbiome_of_beef_cattle_206 PRJNA448333 SRR8416070 PRJNA448333 SRR8397884 cattle_rumen +rumen_microbiome_of_beef_cattle_207 PRJNA448333 SRR8416067 PRJNA448333 SRR8397883 cattle_rumen +rumen_microbiome_of_beef_cattle_208 PRJNA448333 SRR8416068 PRJNA448333 SRR8397886 cattle_rumen +rumen_microbiome_of_beef_cattle_209 PRJNA448333 SRR8416073 PRJNA448333 SRR8397885 cattle_rumen +rumen_microbiome_of_beef_cattle_210 PRJNA448333 SRR8416074 PRJNA448333 SRR8397888 cattle_rumen +rumen_microbiome_of_beef_cattle_301 PRJNA448333 SRR8416071 PRJNA448333 SRR8397887 cattle_rumen +rumen_microbiome_of_beef_cattle_302 PRJNA448333 SRR8416072 PRJNA448333 SRR8397890 cattle_rumen 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PRJNA797778 SRR17858183 PRJNA797778 SRR17635806 human_vagina +UAB052_W1D5 PRJNA797778 SRR17858184 PRJNA797778 SRR17635769 human_vagina +UAB048_W9D7 PRJNA797778 SRR17858185 PRJNA797778 SRR17635771 human_vagina +UAB048_W7D7 PRJNA797778 SRR17858186 PRJNA797778 SRR17635772 human_vagina +UAB048_W5D2 PRJNA797778 SRR17858187 PRJNA797778 SRR17635773 human_vagina +UAB048_W3D7 PRJNA797778 SRR17858188 PRJNA797778 SRR17635774 human_vagina +UAB048_W1D7 PRJNA797778 SRR17858189 PRJNA797778 SRR17635775 human_vagina +UAB044_W9D7 PRJNA797778 SRR17858190 PRJNA797778 SRR17635776 human_vagina +UAB044_W7D7 PRJNA797778 SRR17858191 PRJNA797778 SRR17635777 human_vagina +UAB044_W5D7 PRJNA797778 SRR17858192 PRJNA797778 SRR17635778 human_vagina +UAB044_W3D7 PRJNA797778 SRR17858193 PRJNA797778 SRR17635779 human_vagina +AYAC09_W5D7 PRJNA797778 SRR17858194 PRJNA797778 SRR17635747 human_vagina +UAB044_W1D7 PRJNA797778 SRR17858195 PRJNA797778 SRR17635780 human_vagina +UAB028_W9D7 PRJNA797778 SRR17858196 PRJNA797778 SRR17635598 human_vagina +UAB028_W7D7 PRJNA797778 SRR17858197 PRJNA797778 SRR17635599 human_vagina +UAB022_W10D1 PRJNA797778 SRR17858198 PRJNA797778 SRR17635466 human_vagina +UAB022_W6D1 PRJNA797778 SRR17858199 PRJNA797778 SRR17635468 human_vagina +UAB021_W8D3 PRJNA797778 SRR17858200 PRJNA797778 SRR17635471 human_vagina +UAB021_W5D7 PRJNA797778 SRR17858201 PRJNA797778 SRR17635472 human_vagina +UAB015_W10D7 PRJNA797778 SRR17858202 PRJNA797778 SRR17635475 human_vagina +UAB015_W9D4 PRJNA797778 SRR17858203 PRJNA797778 SRR17635477 human_vagina +UAB011_W9D7 PRJNA797778 SRR17858204 PRJNA797778 SRR17635481 human_vagina +AYAC03_W4D7 PRJNA797778 SRR17858205 PRJNA797778 SRR17635796 human_vagina +UAB011_W7D7 PRJNA797778 SRR17858206 PRJNA797778 SRR17635482 human_vagina +UAB011_W7D1 PRJNA797778 SRR17858207 PRJNA797778 SRR17635483 human_vagina +UAB039_W9D7 PRJNA797778 SRR17858208 PRJNA797778 SRR17635782 human_vagina +UAB039_W7D7 PRJNA797778 SRR17858209 PRJNA797778 SRR17635783 human_vagina 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PRJNA797778 SRR17635597 human_vagina +AYAC03_W7D5 PRJNA797778 SRR17858224 PRJNA797778 SRR17635487 human_vagina +AYAC02_W7D5 PRJNA797778 SRR17858225 PRJNA797778 SRR17635635 human_vagina +UAB010_W7D5 PRJNA797778 SRR17858226 PRJNA797778 SRR17635489 human_vagina +AYAC02_W5D6 PRJNA797778 SRR17858227 PRJNA797778 SRR17635576 human_vagina +UAB007_W9D7 PRJNA797778 SRR17858228 PRJNA797778 SRR17635493 human_vagina +UAB007_W7D7 PRJNA797778 SRR17858229 PRJNA797778 SRR17635494 human_vagina +UAB007_W6D3 PRJNA797778 SRR17858230 PRJNA797778 SRR17635495 human_vagina +UAB006_W9D7 PRJNA797778 SRR17858231 PRJNA797778 SRR17635797 human_vagina +UAB006_W7D7 PRJNA797778 SRR17858232 PRJNA797778 SRR17635798 human_vagina +UAB002_W9D7 PRJNA797778 SRR17858233 PRJNA797778 SRR17635802 human_vagina +UAB002_W7D7 PRJNA797778 SRR17858234 PRJNA797778 SRR17635803 human_vagina +UAB028_W5D5 PRJNA797778 SRR17858235 PRJNA797778 SRR17635600 human_vagina +UAB028_W3D7 PRJNA797778 SRR17858236 PRJNA797778 SRR17635601 human_vagina 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PRJNA797778 SRR17635810 human_vagina +UAB021_W3D7 PRJNA797778 SRR17858251 PRJNA797778 SRR17635473 human_vagina +EM11_W3D6 PRJNA797778 SRR17858252 PRJNA797778 SRR17635811 human_vagina +EM11_W1D7 PRJNA797778 SRR17858253 PRJNA797778 SRR17635812 human_vagina +EM10_W8D7 PRJNA797778 SRR17858254 PRJNA797778 SRR17635813 human_vagina +EM10_W6D5 PRJNA797778 SRR17858255 PRJNA797778 SRR17635814 human_vagina +EM10_W4D7 PRJNA797778 SRR17858256 PRJNA797778 SRR17635815 human_vagina +EM10_W3D2 PRJNA797778 SRR17858257 PRJNA797778 SRR17635816 human_vagina +EM10_W1D7 PRJNA797778 SRR17858258 PRJNA797778 SRR17635817 human_vagina +EM09_W9D6 PRJNA797778 SRR17858259 PRJNA797778 SRR17635819 human_vagina +EM09_W8D5 PRJNA797778 SRR17858260 PRJNA797778 SRR17635820 human_vagina +EM09_W6D7 PRJNA797778 SRR17858261 PRJNA797778 SRR17635459 human_vagina +UAB015_W3D1 PRJNA797778 SRR17858262 PRJNA797778 SRR17635479 human_vagina +AYAC05_W9D7 PRJNA797778 SRR17858263 PRJNA797778 SRR17635684 human_vagina +AYAC05_W7D7 PRJNA797778 SRR17858264 PRJNA797778 SRR17635695 human_vagina +AYAC03_W9D7 PRJNA797778 SRR17858265 PRJNA797778 SRR17635465 human_vagina +AYAC03_W8D6 PRJNA797778 SRR17858266 PRJNA797778 SRR17635476 human_vagina +AYAC09_W3D4 PRJNA797778 SRR17858267 PRJNA797778 SRR17635758 human_vagina +AYAC09_W1D7 PRJNA797778 SRR17858268 PRJNA797778 SRR17635673 human_vagina +AYAC05_W5D5 PRJNA797778 SRR17858269 PRJNA797778 SRR17635770 human_vagina +AYAC05_W3D7 PRJNA797778 SRR17858270 PRJNA797778 SRR17635781 human_vagina +AYAC05_W1D6 PRJNA797778 SRR17858271 PRJNA797778 SRR17635792 human_vagina +EM09_W4D4 PRJNA797778 SRR17858272 PRJNA797778 SRR17635462 human_vagina +UAB015_W1D5 PRJNA797778 SRR17858273 PRJNA797778 SRR17635480 human_vagina +EM09_W2D3 PRJNA797778 SRR17858274 PRJNA797778 SRR17635611 human_vagina +EM04_W1D7 PRJNA797778 SRR17858275 PRJNA797778 SRR17635722 human_vagina +UAB129_W10D2 PRJNA797778 SRR17858276 PRJNA797778 SRR17635577 human_vagina +UAB129_W7D7 PRJNA797778 SRR17858277 PRJNA797778 SRR17635578 human_vagina +UAB129_W5D7 PRJNA797778 SRR17858278 PRJNA797778 SRR17635579 human_vagina +UAB129_W3D3 PRJNA797778 SRR17858279 PRJNA797778 SRR17635580 human_vagina +UAB129_W1D7 PRJNA797778 SRR17858280 PRJNA797778 SRR17635581 human_vagina +UAB122_W9D6 PRJNA797778 SRR17858281 PRJNA797778 SRR17635582 human_vagina +UAB122_W6D5 PRJNA797778 SRR17858282 PRJNA797778 SRR17635583 human_vagina +UAB122_W4D7 PRJNA797778 SRR17858283 PRJNA797778 SRR17635584 human_vagina +UAB011_W3D6 PRJNA797778 SRR17858284 PRJNA797778 SRR17635484 human_vagina +UAB122_W3D4 PRJNA797778 SRR17858285 PRJNA797778 SRR17635585 human_vagina +UAB122_W1D7 PRJNA797778 SRR17858286 PRJNA797778 SRR17635586 human_vagina +UAB119_W9D7 PRJNA797778 SRR17858287 PRJNA797778 SRR17635588 human_vagina +UAB119_W7D7 PRJNA797778 SRR17858288 PRJNA797778 SRR17635589 human_vagina +UAB119_W5D7 PRJNA797778 SRR17858289 PRJNA797778 SRR17635590 human_vagina +UAB119_W3D7 PRJNA797778 SRR17858290 PRJNA797778 SRR17635591 human_vagina +UAB119_W1D7 PRJNA797778 SRR17858291 PRJNA797778 SRR17635592 human_vagina +UAB117_W9D6 PRJNA797778 SRR17858292 PRJNA797778 SRR17635731 human_vagina +UAB117_W8D1 PRJNA797778 SRR17858293 PRJNA797778 SRR17635732 human_vagina +UAB117_W5D6 PRJNA797778 SRR17858294 PRJNA797778 SRR17635733 human_vagina +UAB011_W1D7 PRJNA797778 SRR17858295 PRJNA797778 SRR17635485 human_vagina +UAB117_W3D6 PRJNA797778 SRR17858296 PRJNA797778 SRR17635734 human_vagina +UAB117_W1D6 PRJNA797778 SRR17858297 PRJNA797778 SRR17635735 human_vagina +UAB116_W9D6 PRJNA797778 SRR17858298 PRJNA797778 SRR17635737 human_vagina +UAB116_W6D7 PRJNA797778 SRR17858299 PRJNA797778 SRR17635738 human_vagina +UAB116_W4D6 PRJNA797778 SRR17858300 PRJNA797778 SRR17635739 human_vagina +UAB116_W1D7 PRJNA797778 SRR17858301 PRJNA797778 SRR17635741 human_vagina +UAB110_W10D4 PRJNA797778 SRR17858302 PRJNA797778 SRR17635742 human_vagina +UAB110_W7D7 PRJNA797778 SRR17858303 PRJNA797778 SRR17635743 human_vagina +UAB110_W6D2 PRJNA797778 SRR17858304 PRJNA797778 SRR17635744 human_vagina +UAB110_W3D7 PRJNA797778 SRR17858305 PRJNA797778 SRR17635745 human_vagina +UAB010_W5D6 PRJNA797778 SRR17858306 PRJNA797778 SRR17635490 human_vagina +UAB110_W1D7 PRJNA797778 SRR17858307 PRJNA797778 SRR17635746 human_vagina +UAB106_W9D7 PRJNA797778 SRR17858308 PRJNA797778 SRR17635748 human_vagina +UAB106_W7D7 PRJNA797778 SRR17858309 PRJNA797778 SRR17635749 human_vagina +UAB106_W5D4 PRJNA797778 SRR17858310 PRJNA797778 SRR17635750 human_vagina +UAB106_W3D7 PRJNA797778 SRR17858311 PRJNA797778 SRR17635751 human_vagina +UAB106_W1D7 PRJNA797778 SRR17858312 PRJNA797778 SRR17635752 human_vagina +UAB096_W9D7 PRJNA797778 SRR17858313 PRJNA797778 SRR17635753 human_vagina +UAB096_W7D3 PRJNA797778 SRR17858314 PRJNA797778 SRR17635754 human_vagina +UAB096_W5D3 PRJNA797778 SRR17858315 PRJNA797778 SRR17635755 human_vagina +UAB096_W3D3 PRJNA797778 SRR17858316 PRJNA797778 SRR17635756 human_vagina +UAB010_W3D1 PRJNA797778 SRR17858317 PRJNA797778 SRR17635491 human_vagina +UAB096_W1D5 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SRR4052039 human_gut +MV_FEI5_t3Q15 PRJNA339914 SRR4100707 PRJNA339914 SRR4052042 human_gut +MV_FEM4_t2Q15 PRJNA339914 SRR4100708 PRJNA339914 SRR4052025 human_gut +MV_FEM5_t3Q15 PRJNA339914 SRR4100709 PRJNA339914 SRR4052028 human_gut +M1 PRJEB33889 ERR3473664 PRJEB33889 ERR3473656 mouse_cecum +M2 PRJEB33889 ERR3473665 PRJEB33889 ERR3473657 mouse_cecum +M3 PRJEB33889 ERR3473666 PRJEB33889 ERR3473658 mouse_cecum +M4 PRJEB33889 ERR3473667 PRJEB33889 ERR3473659 mouse_cecum +M5 PRJEB33889 ERR3473668 PRJEB33889 ERR3473660 mouse_cecum +M6 PRJEB33889 ERR3473669 PRJEB33889 ERR3473661 mouse_cecum +M7 PRJEB33889 ERR3473670 PRJEB33889 ERR3473662 mouse_cecum +M8 PRJEB33889 ERR3473671 PRJEB33889 ERR3473663 mouse_cecum +ad_supplemented_with_nitrogen_data_2 PRJNA698464 SRR13618127 PRJNA698464 SRR13618123 bioreactor +ad_supplemented_with_nitrogen_data_1 PRJNA698464 SRR13618128 PRJNA698464 SRR13618124 bioreactor +ad_supplemented_with_hydrogen_data_2 PRJNA698464 SRR13618129 PRJNA698464 SRR13618125 bioreactor +ad_supplemented_with_hydrogen_data_1 PRJNA698464 SRR13618130 PRJNA698464 SRR13618126 bioreactor +X4074 PRJNA396840 SRR5892181 PRJNA396840 SRR5892217 human_oral +X4080 PRJNA396840 SRR5892182 PRJNA396840 SRR5892216 human_oral +X4068 PRJNA396840 SRR5892183 PRJNA396840 SRR5892215 human_oral +X4072 PRJNA396840 SRR5892184 PRJNA396840 SRR5892214 human_oral +X4060 PRJNA396840 SRR5892185 PRJNA396840 SRR5892213 human_oral +X4064 PRJNA396840 SRR5892186 PRJNA396840 SRR5892212 human_oral +X4050 PRJNA396840 SRR5892187 PRJNA396840 SRR5892211 human_oral +X4056 PRJNA396840 SRR5892188 PRJNA396840 SRR5892210 human_oral +X4108 PRJNA396840 SRR5892189 PRJNA396840 SRR5892233 human_oral +X4082 PRJNA396840 SRR5892190 PRJNA396840 SRR5892209 human_oral +X4088 PRJNA396840 SRR5892191 PRJNA396840 SRR5892208 human_oral +X4124 PRJNA396840 SRR5892192 PRJNA396840 SRR5892236 human_oral +X4120 PRJNA396840 SRR5892195 PRJNA396840 SRR5892235 human_oral +X4096 PRJNA396840 SRR5892200 PRJNA396840 SRR5892238 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+ES_08072017_CAT2012October PRJEB12234 ERR2088998 PRJEB12234 ERR2094670 ocean +ES_08072017_CAT2013January PRJEB12234 ERR2088999 PRJEB12234 ERR2094671 ocean +ES_08072017_CAT2013April PRJEB12234 ERR2089000 PRJEB12234 ERR2094672 ocean +ES_08072017_POLA2012July PRJEB12234 ERR2089001 PRJEB12234 ERR2094673 ocean +ES_08072017_POLA2012October PRJEB12234 ERR2089002 PRJEB12234 ERR2094674 ocean +ES_08072017_POLA2013January PRJEB12234 ERR2089003 PRJEB12234 ERR2094675 ocean +ES_08072017_POLA2013April PRJEB12234 ERR2089004 PRJEB12234 ERR2094676 ocean +ES_08072017_SPOT2012July PRJEB12234 ERR2089005 PRJEB12234 ERR2094677 ocean +ES_08072017_SPOT2012October PRJEB12234 ERR2089006 PRJEB12234 ERR2094678 ocean +ES_08072017_SPOT2013January PRJEB12234 ERR2089007 PRJEB12234 ERR2094679 ocean +ES_08072017_SPOT2013April PRJEB12234 ERR2089008 PRJEB12234 ERR2094680 ocean +ES_08072017_neg_reads PRJEB12234 ERR2089009 PRJEB12234 ERR2097133 ocean +E2A PRJNA340003 SRR4342131 PRJNA340003 SRR4342135 ocean +E4 PRJNA340003 SRR4342132 PRJNA340003 SRR4342136 ocean +D1 PRJNA340003 SRR4342137 PRJNA340003 SRR4342129 ocean +D2 PRJNA340003 SRR4342138 PRJNA340003 SRR4342130 ocean +D3 PRJNA340003 SRR4342139 PRJNA340003 SRR4342133 ocean +E2 PRJNA340003 SRR4342140 PRJNA340003 SRR4342134 ocean diff --git a/inputs/metadata-parse-paired-mgx-mtx.ipynb b/inputs/metadata-parse-paired-mgx-mtx.ipynb new file mode 100644 index 0000000..3d17930 --- /dev/null +++ b/inputs/metadata-parse-paired-mgx-mtx.ipynb @@ -0,0 +1,5114 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "id": "3910cfa9", + "metadata": {}, + "source": [ + "# Generate a metadata table specifying paired metagenomes and metatranscriptomes from diverse biomes using publicly available data\n", + "\n", + "This notebook processes metadata associated with paired metagenome and metatranscriptome samples. \n", + "When possible, the ENA BioProject pages were parsed to determine sample pairs.\n", + "This was done programmatically using the following URL (with more metadata fields specified) which uses the ENA API to resolve a TSV for each run in a specified bioproject.\n", + "\n", + "`\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA406858&result=read_run&fields=study_accession,first_created&format=tsv&download=true&limit=0\"`\n", + "\n", + "\n", + "By and large, each project encoded sample identifiers and paired information differently, so each parsing chunk is unique.\n", + "Given this, I chose not to create a parsing function.\n" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "id": "6232c715", + "metadata": {}, + "outputs": [], + "source": [ + "setwd(\"..\")" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "id": "ca7b2c35", + "metadata": {}, + "outputs": [], + "source": [ + "library(dplyr)\n", + "library(readr)\n", + "library(janitor)" + ] + }, + { + "cell_type": "markdown", + "id": "3206624e", + "metadata": {}, + "source": [ + "## PRJNA406858" + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "b79e6ec7", + "metadata": {}, + "outputs": [], + "source": [ + "prjna406858 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA406858&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 5, + "id": "32481186", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 6 × 3
study_accessionrun_accessionlibrary_name
<chr><chr><chr>
PRJNA406858SRR6032600C_2_cDNA_1
PRJNA406858SRR6032601B_2_1
PRJNA406858SRR6032602C_2_1
PRJNA406858SRR6032603E_2_1
PRJNA406858SRR6032604B_2_cDNA_1
PRJNA406858SRR6032605E_2_cDNA_1
\n" + ], + "text/latex": [ + "A tibble: 6 × 3\n", + "\\begin{tabular}{lll}\n", + " study\\_accession & run\\_accession & library\\_name\\\\\n", + " & & \\\\\n", + "\\hline\n", + "\t PRJNA406858 & SRR6032600 & C\\_2\\_cDNA\\_1\\\\\n", + "\t PRJNA406858 & SRR6032601 & B\\_2\\_1 \\\\\n", + "\t PRJNA406858 & SRR6032602 & C\\_2\\_1 \\\\\n", + "\t PRJNA406858 & SRR6032603 & E\\_2\\_1 \\\\\n", + "\t PRJNA406858 & SRR6032604 & B\\_2\\_cDNA\\_1\\\\\n", + "\t PRJNA406858 & SRR6032605 & E\\_2\\_cDNA\\_1\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 6 × 3\n", + "\n", + "| study_accession <chr> | run_accession <chr> | library_name <chr> |\n", + "|---|---|---|\n", + "| PRJNA406858 | SRR6032600 | C_2_cDNA_1 |\n", + "| PRJNA406858 | SRR6032601 | B_2_1 |\n", + "| PRJNA406858 | SRR6032602 | C_2_1 |\n", + "| PRJNA406858 | SRR6032603 | E_2_1 |\n", + "| PRJNA406858 | SRR6032604 | B_2_cDNA_1 |\n", + "| PRJNA406858 | SRR6032605 | E_2_cDNA_1 |\n", + "\n" + ], + "text/plain": [ + " study_accession run_accession library_name\n", + "1 PRJNA406858 SRR6032600 C_2_cDNA_1 \n", + "2 PRJNA406858 SRR6032601 B_2_1 \n", + "3 PRJNA406858 SRR6032602 C_2_1 \n", + "4 PRJNA406858 SRR6032603 E_2_1 \n", + "5 PRJNA406858 SRR6032604 B_2_cDNA_1 \n", + "6 PRJNA406858 SRR6032605 E_2_cDNA_1 " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna406858 <- prjna406858 %>% \n", + " arrange(sample_alias) %>%\n", + " select(study_accession, run_accession, library_name)\n", + "\n", + "head(prjna406858)" + ] + }, + { + "cell_type": "code", + "execution_count": 6, + "id": "998e512d", + "metadata": {}, + "outputs": [], + "source": [ + "prjna406858 <- prjna406858 %>%\n", + " mutate(sample_name = gsub(\"_cDNA\", \"\", library_name))" + ] + }, + { + "cell_type": "code", + "execution_count": 7, + "id": "59810eff", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 3 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
C_2_1PRJNA406858SRR6032600PRJNA406858SRR6032602activated_sludge
B_2_1PRJNA406858SRR6032604PRJNA406858SRR6032601activated_sludge
E_2_1PRJNA406858SRR6032605PRJNA406858SRR6032603activated_sludge
\n" + ], + "text/latex": [ + "A tibble: 3 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t C\\_2\\_1 & PRJNA406858 & SRR6032600 & PRJNA406858 & SRR6032602 & activated\\_sludge\\\\\n", + "\t B\\_2\\_1 & PRJNA406858 & SRR6032604 & PRJNA406858 & SRR6032601 & activated\\_sludge\\\\\n", + "\t E\\_2\\_1 & PRJNA406858 & SRR6032605 & PRJNA406858 & SRR6032603 & activated\\_sludge\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 3 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| C_2_1 | PRJNA406858 | SRR6032600 | PRJNA406858 | SRR6032602 | activated_sludge |\n", + "| B_2_1 | PRJNA406858 | SRR6032604 | PRJNA406858 | SRR6032601 | activated_sludge |\n", + "| E_2_1 | PRJNA406858 | SRR6032605 | PRJNA406858 | SRR6032603 | activated_sludge |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 C_2_1 PRJNA406858 SRR6032600 PRJNA406858 \n", + "2 B_2_1 PRJNA406858 SRR6032604 PRJNA406858 \n", + "3 E_2_1 PRJNA406858 SRR6032605 PRJNA406858 \n", + " mgx_run_accession sample_type \n", + "1 SRR6032602 activated_sludge\n", + "2 SRR6032601 activated_sludge\n", + "3 SRR6032603 activated_sludge" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna406858_mtx <- prjna406858 %>%\n", + " filter(grepl(\"cDNA\", library_name)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna406858_mgx <- prjna406858 %>%\n", + " filter(!grepl(\"cDNA\", library_name)) %>%\n", + " select(mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession, \n", + " sample_name)\n", + "\n", + "prjna406858_mtx_vs_mgx <- left_join(prjna406858_mtx, prjna406858_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"activated_sludge\")\n", + "\n", + "prjna406858_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "efa62e72", + "metadata": {}, + "source": [ + "## PRJNA448333" + ] + }, + { + "cell_type": "code", + "execution_count": 8, + "id": "ab5a6d12", + "metadata": {}, + "outputs": [], + "source": [ + "prjna448333 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA448333&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 9, + "id": "3a4dec6c", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 6 × 4
study_accessionrun_accessionlibrary_namesample_alias
<chr><chr><chr><chr>
PRJNA448333SRR8397906Metagenome 101 Rumen microbiome of beef cattle 101
PRJNA448333SRR8399431Total-RNA-based metatranscriptome 101Rumen microbiome of beef cattle 101
PRJNA448333SRR8416057mRNA-enriched metatranscriptome 101 Rumen microbiome of beef cattle 101
PRJNA448333SRR8404214Metagenome 103 Rumen microbiome of beef cattle 103
PRJNA448333SRR8416058mRNA-enriched metatranscriptome 103 Rumen microbiome of beef cattle 103
PRJNA448333SRR8420492Total-RNA-based metatranscriptome 103Rumen microbiome of beef cattle 103
\n" + ], + "text/latex": [ + "A tibble: 6 × 4\n", + "\\begin{tabular}{llll}\n", + " study\\_accession & run\\_accession & library\\_name & sample\\_alias\\\\\n", + " & & & \\\\\n", + "\\hline\n", + "\t PRJNA448333 & SRR8397906 & Metagenome 101 & Rumen microbiome of beef cattle 101\\\\\n", + "\t PRJNA448333 & SRR8399431 & Total-RNA-based metatranscriptome 101 & Rumen microbiome of beef cattle 101\\\\\n", + "\t PRJNA448333 & SRR8416057 & mRNA-enriched metatranscriptome 101 & Rumen microbiome of beef cattle 101\\\\\n", + "\t PRJNA448333 & SRR8404214 & Metagenome 103 & Rumen microbiome of beef cattle 103\\\\\n", + "\t PRJNA448333 & SRR8416058 & mRNA-enriched metatranscriptome 103 & Rumen microbiome of beef cattle 103\\\\\n", + "\t PRJNA448333 & SRR8420492 & Total-RNA-based metatranscriptome 103 & Rumen microbiome of beef cattle 103\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 6 × 4\n", + "\n", + "| study_accession <chr> | run_accession <chr> | library_name <chr> | sample_alias <chr> |\n", + "|---|---|---|---|\n", + "| PRJNA448333 | SRR8397906 | Metagenome 101 | Rumen microbiome of beef cattle 101 |\n", + "| PRJNA448333 | SRR8399431 | Total-RNA-based metatranscriptome 101 | Rumen microbiome of beef cattle 101 |\n", + "| PRJNA448333 | SRR8416057 | mRNA-enriched metatranscriptome 101 | Rumen microbiome of beef cattle 101 |\n", + "| PRJNA448333 | SRR8404214 | Metagenome 103 | Rumen microbiome of beef cattle 103 |\n", + "| PRJNA448333 | SRR8416058 | mRNA-enriched metatranscriptome 103 | Rumen microbiome of beef cattle 103 |\n", + "| PRJNA448333 | SRR8420492 | Total-RNA-based metatranscriptome 103 | Rumen microbiome of beef cattle 103 |\n", + "\n" + ], + "text/plain": [ + " study_accession run_accession library_name \n", + "1 PRJNA448333 SRR8397906 Metagenome 101 \n", + "2 PRJNA448333 SRR8399431 Total-RNA-based metatranscriptome 101\n", + "3 PRJNA448333 SRR8416057 mRNA-enriched metatranscriptome 101 \n", + "4 PRJNA448333 SRR8404214 Metagenome 103 \n", + "5 PRJNA448333 SRR8416058 mRNA-enriched metatranscriptome 103 \n", + "6 PRJNA448333 SRR8420492 Total-RNA-based metatranscriptome 103\n", + " sample_alias \n", + "1 Rumen microbiome of beef cattle 101\n", + "2 Rumen microbiome of beef cattle 101\n", + "3 Rumen microbiome of beef cattle 101\n", + "4 Rumen microbiome of beef cattle 103\n", + "5 Rumen microbiome of beef cattle 103\n", + "6 Rumen microbiome of beef cattle 103" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna448333 <- prjna448333 %>% \n", + " arrange(sample_alias) %>%\n", + " select(study_accession, run_accession, library_name, sample_alias)\n", + "\n", + "head(prjna448333)" + ] + }, + { + "cell_type": "markdown", + "id": "6cc53500", + "metadata": {}, + "source": [ + "PRJNA448333 has total RNA and mRNA-enriched RNA metatranscriptomes. I included mRNA-enriched samples as these likely contain more of the sequences we're interested in." + ] + }, + { + "cell_type": "code", + "execution_count": 10, + "id": "535d6fcf", + "metadata": {}, + "outputs": [], + "source": [ + "prjna448333_mtx <- prjna448333 %>% \n", + " filter(grepl(\"mRNA-enriched\", library_name)) %>%\n", + " select(sample_name = sample_alias,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna448333_mgx <- prjna448333 %>% \n", + " filter(grepl(\"Metagenome\", library_name)) %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)" + ] + }, + { + "cell_type": "code", + "execution_count": 11, + "id": "958b2644", + "metadata": {}, + "outputs": [], + "source": [ + "prjna448333_mtx_vs_mgx <- left_join(prjna448333_mtx, prjna448333_mgx, by = c(\"sample_name\")) %>%\n", + " mutate(sample_name = make_clean_names(sample_name),\n", + " sample_type = \"cattle_rumen\")" + ] + }, + { + "cell_type": "code", + "execution_count": 12, + "id": "3da0c395", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 48 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
rumen_microbiome_of_beef_cattle_101PRJNA448333SRR8416057PRJNA448333SRR8397906cattle_rumen
rumen_microbiome_of_beef_cattle_103PRJNA448333SRR8416058PRJNA448333SRR8404214cattle_rumen
rumen_microbiome_of_beef_cattle_104PRJNA448333SRR8416055PRJNA448333SRR8397905cattle_rumen
rumen_microbiome_of_beef_cattle_105PRJNA448333SRR8416056PRJNA448333SRR8397904cattle_rumen
rumen_microbiome_of_beef_cattle_106PRJNA448333SRR8416061PRJNA448333SRR8397903cattle_rumen
rumen_microbiome_of_beef_cattle_107PRJNA448333SRR8416062PRJNA448333SRR8397910cattle_rumen
rumen_microbiome_of_beef_cattle_112PRJNA448333SRR8416059PRJNA448333SRR8397909cattle_rumen
rumen_microbiome_of_beef_cattle_201PRJNA448333SRR8416060PRJNA448333SRR8397908cattle_rumen
rumen_microbiome_of_beef_cattle_202PRJNA448333SRR8416064PRJNA448333SRR8397907cattle_rumen
rumen_microbiome_of_beef_cattle_203PRJNA448333SRR8416065PRJNA448333SRR8397902cattle_rumen
rumen_microbiome_of_beef_cattle_205PRJNA448333SRR8416069PRJNA448333SRR8397901cattle_rumen
rumen_microbiome_of_beef_cattle_206PRJNA448333SRR8416070PRJNA448333SRR8397884cattle_rumen
rumen_microbiome_of_beef_cattle_207PRJNA448333SRR8416067PRJNA448333SRR8397883cattle_rumen
rumen_microbiome_of_beef_cattle_208PRJNA448333SRR8416068PRJNA448333SRR8397886cattle_rumen
rumen_microbiome_of_beef_cattle_209PRJNA448333SRR8416073PRJNA448333SRR8397885cattle_rumen
rumen_microbiome_of_beef_cattle_210PRJNA448333SRR8416074PRJNA448333SRR8397888cattle_rumen
rumen_microbiome_of_beef_cattle_301PRJNA448333SRR8416071PRJNA448333SRR8397887cattle_rumen
rumen_microbiome_of_beef_cattle_302PRJNA448333SRR8416072PRJNA448333SRR8397890cattle_rumen
rumen_microbiome_of_beef_cattle_303PRJNA448333SRR8416075PRJNA448333SRR8397889cattle_rumen
rumen_microbiome_of_beef_cattle_304PRJNA448333SRR8416076PRJNA448333SRR8397882cattle_rumen
rumen_microbiome_of_beef_cattle_305PRJNA448333SRR8416092PRJNA448333SRR8397881cattle_rumen
rumen_microbiome_of_beef_cattle_307PRJNA448333SRR8416091PRJNA448333SRR8397917cattle_rumen
rumen_microbiome_of_beef_cattle_308PRJNA448333SRR8416090PRJNA448333SRR8397918cattle_rumen
rumen_microbiome_of_beef_cattle_401PRJNA448333SRR8416089PRJNA448333SRR8397919cattle_rumen
rumen_microbiome_of_beef_cattle_402PRJNA448333SRR8416096PRJNA448333SRR8397920cattle_rumen
rumen_microbiome_of_beef_cattle_403PRJNA448333SRR8416095PRJNA448333SRR8397913cattle_rumen
rumen_microbiome_of_beef_cattle_404PRJNA448333SRR8416094PRJNA448333SRR8397914cattle_rumen
rumen_microbiome_of_beef_cattle_407PRJNA448333SRR8416093PRJNA448333SRR8397915cattle_rumen
rumen_microbiome_of_beef_cattle_501PRJNA448333SRR8416088PRJNA448333SRR8397916cattle_rumen
rumen_microbiome_of_beef_cattle_503PRJNA448333SRR8416087PRJNA448333SRR8397911cattle_rumen
rumen_microbiome_of_beef_cattle_505PRJNA448333SRR8416102PRJNA448333SRR8397912cattle_rumen
rumen_microbiome_of_beef_cattle_506PRJNA448333SRR8416083PRJNA448333SRR8397894cattle_rumen
rumen_microbiome_of_beef_cattle_601PRJNA448333SRR8416063PRJNA448333SRR8397893cattle_rumen
rumen_microbiome_of_beef_cattle_604PRJNA448333SRR8416066PRJNA448333SRR8397892cattle_rumen
rumen_microbiome_of_beef_cattle_608PRJNA448333SRR8416097PRJNA448333SRR8397891cattle_rumen
rumen_microbiome_of_beef_cattle_611PRJNA448333SRR8416098PRJNA448333SRR8397898cattle_rumen
rumen_microbiome_of_beef_cattle_612PRJNA448333SRR8416099PRJNA448333SRR8397897cattle_rumen
rumen_microbiome_of_beef_cattle_701PRJNA448333SRR8416100PRJNA448333SRR8397896cattle_rumen
rumen_microbiome_of_beef_cattle_702PRJNA448333SRR8416077PRJNA448333SRR8397895cattle_rumen
rumen_microbiome_of_beef_cattle_703PRJNA448333SRR8416078PRJNA448333SRR8397900cattle_rumen
rumen_microbiome_of_beef_cattle_704PRJNA448333SRR8416082PRJNA448333SRR8397899cattle_rumen
rumen_microbiome_of_beef_cattle_705PRJNA448333SRR8416081PRJNA448333SRR8397926cattle_rumen
rumen_microbiome_of_beef_cattle_706PRJNA448333SRR8416086PRJNA448333SRR8397927cattle_rumen
rumen_microbiome_of_beef_cattle_707PRJNA448333SRR8416084PRJNA448333SRR8397924cattle_rumen
rumen_microbiome_of_beef_cattle_708PRJNA448333SRR8416101PRJNA448333SRR8397925cattle_rumen
rumen_microbiome_of_beef_cattle_709PRJNA448333SRR8416085PRJNA448333SRR8397922cattle_rumen
rumen_microbiome_of_beef_cattle_710PRJNA448333SRR8416080PRJNA448333SRR8397923cattle_rumen
rumen_microbiome_of_beef_cattle_712PRJNA448333SRR8416079PRJNA448333SRR8397921cattle_rumen
\n" + ], + "text/latex": [ + "A tibble: 48 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_101 & PRJNA448333 & SRR8416057 & PRJNA448333 & SRR8397906 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_103 & PRJNA448333 & SRR8416058 & PRJNA448333 & SRR8404214 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_104 & PRJNA448333 & SRR8416055 & PRJNA448333 & SRR8397905 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_105 & PRJNA448333 & SRR8416056 & PRJNA448333 & SRR8397904 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_106 & PRJNA448333 & SRR8416061 & PRJNA448333 & SRR8397903 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_107 & PRJNA448333 & SRR8416062 & PRJNA448333 & SRR8397910 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_112 & PRJNA448333 & SRR8416059 & PRJNA448333 & SRR8397909 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_201 & PRJNA448333 & SRR8416060 & PRJNA448333 & SRR8397908 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_202 & PRJNA448333 & SRR8416064 & PRJNA448333 & SRR8397907 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_203 & PRJNA448333 & SRR8416065 & PRJNA448333 & SRR8397902 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_205 & PRJNA448333 & SRR8416069 & PRJNA448333 & SRR8397901 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_206 & PRJNA448333 & SRR8416070 & PRJNA448333 & SRR8397884 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_207 & PRJNA448333 & SRR8416067 & PRJNA448333 & SRR8397883 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_208 & PRJNA448333 & SRR8416068 & PRJNA448333 & SRR8397886 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_209 & PRJNA448333 & SRR8416073 & PRJNA448333 & SRR8397885 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_210 & PRJNA448333 & SRR8416074 & PRJNA448333 & SRR8397888 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_301 & PRJNA448333 & SRR8416071 & PRJNA448333 & SRR8397887 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_302 & PRJNA448333 & SRR8416072 & PRJNA448333 & SRR8397890 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_303 & PRJNA448333 & SRR8416075 & PRJNA448333 & SRR8397889 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_304 & PRJNA448333 & SRR8416076 & PRJNA448333 & SRR8397882 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_305 & PRJNA448333 & SRR8416092 & PRJNA448333 & SRR8397881 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_307 & PRJNA448333 & SRR8416091 & PRJNA448333 & SRR8397917 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_308 & PRJNA448333 & SRR8416090 & PRJNA448333 & SRR8397918 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_401 & PRJNA448333 & SRR8416089 & PRJNA448333 & SRR8397919 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_402 & PRJNA448333 & SRR8416096 & PRJNA448333 & SRR8397920 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_403 & PRJNA448333 & SRR8416095 & PRJNA448333 & SRR8397913 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_404 & PRJNA448333 & SRR8416094 & PRJNA448333 & SRR8397914 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_407 & PRJNA448333 & SRR8416093 & PRJNA448333 & SRR8397915 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_501 & PRJNA448333 & SRR8416088 & PRJNA448333 & SRR8397916 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_503 & PRJNA448333 & SRR8416087 & PRJNA448333 & SRR8397911 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_505 & PRJNA448333 & SRR8416102 & PRJNA448333 & SRR8397912 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_506 & PRJNA448333 & SRR8416083 & PRJNA448333 & SRR8397894 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_601 & PRJNA448333 & SRR8416063 & PRJNA448333 & SRR8397893 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_604 & PRJNA448333 & SRR8416066 & PRJNA448333 & SRR8397892 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_608 & PRJNA448333 & SRR8416097 & PRJNA448333 & SRR8397891 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_611 & PRJNA448333 & SRR8416098 & PRJNA448333 & SRR8397898 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_612 & PRJNA448333 & SRR8416099 & PRJNA448333 & SRR8397897 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_701 & PRJNA448333 & SRR8416100 & PRJNA448333 & SRR8397896 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_702 & PRJNA448333 & SRR8416077 & PRJNA448333 & SRR8397895 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_703 & PRJNA448333 & SRR8416078 & PRJNA448333 & SRR8397900 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_704 & PRJNA448333 & SRR8416082 & PRJNA448333 & SRR8397899 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_705 & PRJNA448333 & SRR8416081 & PRJNA448333 & SRR8397926 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_706 & PRJNA448333 & SRR8416086 & PRJNA448333 & SRR8397927 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_707 & PRJNA448333 & SRR8416084 & PRJNA448333 & SRR8397924 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_708 & PRJNA448333 & SRR8416101 & PRJNA448333 & SRR8397925 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_709 & PRJNA448333 & SRR8416085 & PRJNA448333 & SRR8397922 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_710 & PRJNA448333 & SRR8416080 & PRJNA448333 & SRR8397923 & cattle\\_rumen\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_712 & PRJNA448333 & SRR8416079 & PRJNA448333 & SRR8397921 & cattle\\_rumen\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 48 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| rumen_microbiome_of_beef_cattle_101 | PRJNA448333 | SRR8416057 | PRJNA448333 | SRR8397906 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_103 | PRJNA448333 | SRR8416058 | PRJNA448333 | SRR8404214 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_104 | PRJNA448333 | SRR8416055 | PRJNA448333 | SRR8397905 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_105 | PRJNA448333 | SRR8416056 | PRJNA448333 | SRR8397904 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_106 | PRJNA448333 | SRR8416061 | PRJNA448333 | SRR8397903 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_107 | PRJNA448333 | SRR8416062 | PRJNA448333 | SRR8397910 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_112 | PRJNA448333 | SRR8416059 | PRJNA448333 | SRR8397909 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_201 | PRJNA448333 | SRR8416060 | PRJNA448333 | SRR8397908 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_202 | PRJNA448333 | SRR8416064 | PRJNA448333 | SRR8397907 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_203 | PRJNA448333 | SRR8416065 | PRJNA448333 | SRR8397902 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_205 | PRJNA448333 | SRR8416069 | PRJNA448333 | SRR8397901 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_206 | PRJNA448333 | SRR8416070 | PRJNA448333 | SRR8397884 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_207 | PRJNA448333 | SRR8416067 | PRJNA448333 | SRR8397883 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_208 | PRJNA448333 | SRR8416068 | PRJNA448333 | SRR8397886 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_209 | PRJNA448333 | SRR8416073 | PRJNA448333 | SRR8397885 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_210 | PRJNA448333 | SRR8416074 | PRJNA448333 | SRR8397888 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_301 | PRJNA448333 | SRR8416071 | PRJNA448333 | SRR8397887 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_302 | PRJNA448333 | SRR8416072 | PRJNA448333 | SRR8397890 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_303 | PRJNA448333 | SRR8416075 | PRJNA448333 | SRR8397889 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_304 | PRJNA448333 | SRR8416076 | PRJNA448333 | SRR8397882 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_305 | PRJNA448333 | SRR8416092 | PRJNA448333 | SRR8397881 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_307 | PRJNA448333 | SRR8416091 | PRJNA448333 | SRR8397917 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_308 | PRJNA448333 | SRR8416090 | PRJNA448333 | SRR8397918 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_401 | PRJNA448333 | SRR8416089 | PRJNA448333 | SRR8397919 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_402 | PRJNA448333 | SRR8416096 | PRJNA448333 | SRR8397920 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_403 | PRJNA448333 | SRR8416095 | PRJNA448333 | SRR8397913 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_404 | PRJNA448333 | SRR8416094 | PRJNA448333 | SRR8397914 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_407 | PRJNA448333 | SRR8416093 | PRJNA448333 | SRR8397915 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_501 | PRJNA448333 | SRR8416088 | PRJNA448333 | SRR8397916 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_503 | PRJNA448333 | SRR8416087 | PRJNA448333 | SRR8397911 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_505 | PRJNA448333 | SRR8416102 | PRJNA448333 | SRR8397912 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_506 | PRJNA448333 | SRR8416083 | PRJNA448333 | SRR8397894 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_601 | PRJNA448333 | SRR8416063 | PRJNA448333 | SRR8397893 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_604 | PRJNA448333 | SRR8416066 | PRJNA448333 | SRR8397892 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_608 | PRJNA448333 | SRR8416097 | PRJNA448333 | SRR8397891 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_611 | PRJNA448333 | SRR8416098 | PRJNA448333 | SRR8397898 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_612 | PRJNA448333 | SRR8416099 | PRJNA448333 | SRR8397897 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_701 | PRJNA448333 | SRR8416100 | PRJNA448333 | SRR8397896 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_702 | PRJNA448333 | SRR8416077 | PRJNA448333 | SRR8397895 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_703 | PRJNA448333 | SRR8416078 | PRJNA448333 | SRR8397900 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_704 | PRJNA448333 | SRR8416082 | PRJNA448333 | SRR8397899 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_705 | PRJNA448333 | SRR8416081 | PRJNA448333 | SRR8397926 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_706 | PRJNA448333 | SRR8416086 | PRJNA448333 | SRR8397927 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_707 | PRJNA448333 | SRR8416084 | PRJNA448333 | SRR8397924 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_708 | PRJNA448333 | SRR8416101 | PRJNA448333 | SRR8397925 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_709 | PRJNA448333 | SRR8416085 | PRJNA448333 | SRR8397922 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_710 | PRJNA448333 | SRR8416080 | PRJNA448333 | SRR8397923 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_712 | PRJNA448333 | SRR8416079 | PRJNA448333 | SRR8397921 | cattle_rumen |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession\n", + "1 rumen_microbiome_of_beef_cattle_101 PRJNA448333 SRR8416057 \n", + "2 rumen_microbiome_of_beef_cattle_103 PRJNA448333 SRR8416058 \n", + "3 rumen_microbiome_of_beef_cattle_104 PRJNA448333 SRR8416055 \n", + "4 rumen_microbiome_of_beef_cattle_105 PRJNA448333 SRR8416056 \n", + "5 rumen_microbiome_of_beef_cattle_106 PRJNA448333 SRR8416061 \n", + "6 rumen_microbiome_of_beef_cattle_107 PRJNA448333 SRR8416062 \n", + "7 rumen_microbiome_of_beef_cattle_112 PRJNA448333 SRR8416059 \n", + "8 rumen_microbiome_of_beef_cattle_201 PRJNA448333 SRR8416060 \n", + "9 rumen_microbiome_of_beef_cattle_202 PRJNA448333 SRR8416064 \n", + "10 rumen_microbiome_of_beef_cattle_203 PRJNA448333 SRR8416065 \n", + "11 rumen_microbiome_of_beef_cattle_205 PRJNA448333 SRR8416069 \n", + "12 rumen_microbiome_of_beef_cattle_206 PRJNA448333 SRR8416070 \n", + "13 rumen_microbiome_of_beef_cattle_207 PRJNA448333 SRR8416067 \n", + "14 rumen_microbiome_of_beef_cattle_208 PRJNA448333 SRR8416068 \n", + "15 rumen_microbiome_of_beef_cattle_209 PRJNA448333 SRR8416073 \n", + "16 rumen_microbiome_of_beef_cattle_210 PRJNA448333 SRR8416074 \n", + "17 rumen_microbiome_of_beef_cattle_301 PRJNA448333 SRR8416071 \n", + "18 rumen_microbiome_of_beef_cattle_302 PRJNA448333 SRR8416072 \n", + "19 rumen_microbiome_of_beef_cattle_303 PRJNA448333 SRR8416075 \n", + "20 rumen_microbiome_of_beef_cattle_304 PRJNA448333 SRR8416076 \n", + "21 rumen_microbiome_of_beef_cattle_305 PRJNA448333 SRR8416092 \n", + "22 rumen_microbiome_of_beef_cattle_307 PRJNA448333 SRR8416091 \n", + "23 rumen_microbiome_of_beef_cattle_308 PRJNA448333 SRR8416090 \n", + "24 rumen_microbiome_of_beef_cattle_401 PRJNA448333 SRR8416089 \n", + "25 rumen_microbiome_of_beef_cattle_402 PRJNA448333 SRR8416096 \n", + "26 rumen_microbiome_of_beef_cattle_403 PRJNA448333 SRR8416095 \n", + "27 rumen_microbiome_of_beef_cattle_404 PRJNA448333 SRR8416094 \n", + "28 rumen_microbiome_of_beef_cattle_407 PRJNA448333 SRR8416093 \n", + "29 rumen_microbiome_of_beef_cattle_501 PRJNA448333 SRR8416088 \n", + "30 rumen_microbiome_of_beef_cattle_503 PRJNA448333 SRR8416087 \n", + "31 rumen_microbiome_of_beef_cattle_505 PRJNA448333 SRR8416102 \n", + "32 rumen_microbiome_of_beef_cattle_506 PRJNA448333 SRR8416083 \n", + "33 rumen_microbiome_of_beef_cattle_601 PRJNA448333 SRR8416063 \n", + "34 rumen_microbiome_of_beef_cattle_604 PRJNA448333 SRR8416066 \n", + "35 rumen_microbiome_of_beef_cattle_608 PRJNA448333 SRR8416097 \n", + "36 rumen_microbiome_of_beef_cattle_611 PRJNA448333 SRR8416098 \n", + "37 rumen_microbiome_of_beef_cattle_612 PRJNA448333 SRR8416099 \n", + "38 rumen_microbiome_of_beef_cattle_701 PRJNA448333 SRR8416100 \n", + "39 rumen_microbiome_of_beef_cattle_702 PRJNA448333 SRR8416077 \n", + "40 rumen_microbiome_of_beef_cattle_703 PRJNA448333 SRR8416078 \n", + "41 rumen_microbiome_of_beef_cattle_704 PRJNA448333 SRR8416082 \n", + "42 rumen_microbiome_of_beef_cattle_705 PRJNA448333 SRR8416081 \n", + "43 rumen_microbiome_of_beef_cattle_706 PRJNA448333 SRR8416086 \n", + "44 rumen_microbiome_of_beef_cattle_707 PRJNA448333 SRR8416084 \n", + "45 rumen_microbiome_of_beef_cattle_708 PRJNA448333 SRR8416101 \n", + "46 rumen_microbiome_of_beef_cattle_709 PRJNA448333 SRR8416085 \n", + "47 rumen_microbiome_of_beef_cattle_710 PRJNA448333 SRR8416080 \n", + "48 rumen_microbiome_of_beef_cattle_712 PRJNA448333 SRR8416079 \n", + " mgx_study_accession mgx_run_accession sample_type \n", + "1 PRJNA448333 SRR8397906 cattle_rumen\n", + "2 PRJNA448333 SRR8404214 cattle_rumen\n", + "3 PRJNA448333 SRR8397905 cattle_rumen\n", + "4 PRJNA448333 SRR8397904 cattle_rumen\n", + "5 PRJNA448333 SRR8397903 cattle_rumen\n", + "6 PRJNA448333 SRR8397910 cattle_rumen\n", + "7 PRJNA448333 SRR8397909 cattle_rumen\n", + "8 PRJNA448333 SRR8397908 cattle_rumen\n", + "9 PRJNA448333 SRR8397907 cattle_rumen\n", + "10 PRJNA448333 SRR8397902 cattle_rumen\n", + "11 PRJNA448333 SRR8397901 cattle_rumen\n", + "12 PRJNA448333 SRR8397884 cattle_rumen\n", + "13 PRJNA448333 SRR8397883 cattle_rumen\n", + "14 PRJNA448333 SRR8397886 cattle_rumen\n", + "15 PRJNA448333 SRR8397885 cattle_rumen\n", + "16 PRJNA448333 SRR8397888 cattle_rumen\n", + "17 PRJNA448333 SRR8397887 cattle_rumen\n", + "18 PRJNA448333 SRR8397890 cattle_rumen\n", + "19 PRJNA448333 SRR8397889 cattle_rumen\n", + "20 PRJNA448333 SRR8397882 cattle_rumen\n", + "21 PRJNA448333 SRR8397881 cattle_rumen\n", + "22 PRJNA448333 SRR8397917 cattle_rumen\n", + "23 PRJNA448333 SRR8397918 cattle_rumen\n", + "24 PRJNA448333 SRR8397919 cattle_rumen\n", + "25 PRJNA448333 SRR8397920 cattle_rumen\n", + "26 PRJNA448333 SRR8397913 cattle_rumen\n", + "27 PRJNA448333 SRR8397914 cattle_rumen\n", + "28 PRJNA448333 SRR8397915 cattle_rumen\n", + "29 PRJNA448333 SRR8397916 cattle_rumen\n", + "30 PRJNA448333 SRR8397911 cattle_rumen\n", + "31 PRJNA448333 SRR8397912 cattle_rumen\n", + "32 PRJNA448333 SRR8397894 cattle_rumen\n", + "33 PRJNA448333 SRR8397893 cattle_rumen\n", + "34 PRJNA448333 SRR8397892 cattle_rumen\n", + "35 PRJNA448333 SRR8397891 cattle_rumen\n", + "36 PRJNA448333 SRR8397898 cattle_rumen\n", + "37 PRJNA448333 SRR8397897 cattle_rumen\n", + "38 PRJNA448333 SRR8397896 cattle_rumen\n", + "39 PRJNA448333 SRR8397895 cattle_rumen\n", + "40 PRJNA448333 SRR8397900 cattle_rumen\n", + "41 PRJNA448333 SRR8397899 cattle_rumen\n", + "42 PRJNA448333 SRR8397926 cattle_rumen\n", + "43 PRJNA448333 SRR8397927 cattle_rumen\n", + "44 PRJNA448333 SRR8397924 cattle_rumen\n", + "45 PRJNA448333 SRR8397925 cattle_rumen\n", + "46 PRJNA448333 SRR8397922 cattle_rumen\n", + "47 PRJNA448333 SRR8397923 cattle_rumen\n", + "48 PRJNA448333 SRR8397921 cattle_rumen" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna448333_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "a6509917", + "metadata": {}, + "source": [ + "## PRJNA344005" + ] + }, + { + "cell_type": "code", + "execution_count": 13, + "id": "543d0709", + "metadata": {}, + "outputs": [], + "source": [ + "prjna344005 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA344005&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F) %>%\n", + " mutate(sample_name = gsub(\"_[DR]NA*.\", \"\", library_name)) " + ] + }, + { + "cell_type": "code", + "execution_count": 14, + "id": "f44db485", + "metadata": {}, + "outputs": [], + "source": [ + "prjna344005_pairs <- prjna344005 %>%\n", + " group_by(sample_name) %>%\n", + " tally() %>%\n", + " filter(n == 2)" + ] + }, + { + "cell_type": "code", + "execution_count": 15, + "id": "7883c2cd", + "metadata": {}, + "outputs": [], + "source": [ + "prjna344005 <- prjna344005 %>%\n", + " filter(sample_name %in% prjna344005_pairs$sample_name) %>%\n", + " select(study_accession, run_accession, sample_name, library_strategy)\n", + "\n", + "prjna344005_mtx <- prjna344005 %>%\n", + " filter(library_strategy == \"RNA-Seq\") %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna344005_mgx <- prjna344005 %>%\n", + " filter(library_strategy == \"WGS\") %>%\n", + " select(sample_name,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)" + ] + }, + { + "cell_type": "code", + "execution_count": 16, + "id": "f8e7fd2f", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 2 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
HiTCE_2d PRJNA344005SRR4308224PRJNA344005SRR4308227groundwater
HiTCEB12_2dPRJNA344005SRR4308225PRJNA344005SRR4308226groundwater
\n" + ], + "text/latex": [ + "A tibble: 2 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t HiTCE\\_2d & PRJNA344005 & SRR4308224 & PRJNA344005 & SRR4308227 & groundwater\\\\\n", + "\t HiTCEB12\\_2d & PRJNA344005 & SRR4308225 & PRJNA344005 & SRR4308226 & groundwater\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 2 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| HiTCE_2d | PRJNA344005 | SRR4308224 | PRJNA344005 | SRR4308227 | groundwater |\n", + "| HiTCEB12_2d | PRJNA344005 | SRR4308225 | PRJNA344005 | SRR4308226 | groundwater |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 HiTCE_2d PRJNA344005 SRR4308224 PRJNA344005 \n", + "2 HiTCEB12_2d PRJNA344005 SRR4308225 PRJNA344005 \n", + " mgx_run_accession sample_type\n", + "1 SRR4308227 groundwater\n", + "2 SRR4308226 groundwater" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna344005_mtx_vs_mgx <- left_join(prjna344005_mtx, prjna344005_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"groundwater\")\n", + "\n", + "prjna344005_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "e0ae555e", + "metadata": {}, + "source": [ + "## PRJNA237345 (mtx) & PRJNA237344 (mgx)" + ] + }, + { + "cell_type": "code", + "execution_count": 17, + "id": "16d89c6e", + "metadata": {}, + "outputs": [], + "source": [ + "prjna237345 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA237345&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)\n", + "prjna237344 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA237344&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 18, + "id": "d9d8f133", + "metadata": {}, + "outputs": [ + { + "name": "stderr", + "output_type": "stream", + "text": [ + "\u001b[1m\u001b[22mJoining, by = \"sample_name\"\n" + ] + }, + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A grouped_df: 23 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
amazon_plume_2_0um_from_station_10 PRJNA237345SRR1193190PRJNA237344SRR1205250river
amazon_plume_2_0um_from_station_2 PRJNA237345SRR1193177PRJNA237344SRR1182511river
amazon_plume_2_0um_from_station_23 PRJNA237345SRR1193237PRJNA237344SRR1202089river
amazon_plume_2_0um_from_station_27 PRJNA237345SRR1193629PRJNA237344SRR1183643river
amazon_plume_2_0um_from_station_3 PRJNA237345SRR1193226PRJNA237344SRR1199272river
amazon_plume_0_2_2_0um_from_station_10 PRJNA237345SRR1186930PRJNA237344SRR1199271river
amazon_plume_0_2_2_0um_from_station_2 PRJNA237345SRR1193205PRJNA237344SRR1182512river
amazon_plume_0_2_2_0um_from_station_23 PRJNA237345SRR1193632PRJNA237344SRR1186214river
amazon_plume_0_2_2_0um_from_station_25 PRJNA237345SRR1204579PRJNA237344SRR1202090river
amazon_plume_0_2_2_0um_from_station_27 PRJNA237345SRR1193627PRJNA237344SRR1183650river
amazon_plume_0_2_2_0um_from_station_3 PRJNA237345SRR1193215PRJNA237344SRR1185413river
amazon_river_0_2_2_0um_from_belem PRJNA237345SRR1781804PRJNA237344SRR1790489river
amazon_river_0_2_2_0um_from_macapa_north_channelPRJNA237345SRR1785209PRJNA237344SRR1786279river
amazon_river_0_2_2_0um_from_macapa_south_channelPRJNA237345SRR1784299PRJNA237344SRR1787940river
amazon_river_0_2_2_0um_from_obidos PRJNA237345SRR1781945PRJNA237344SRR1790676river
amazon_river_0_2_2_0um_from_tapajos_depth PRJNA237345SRR1779221PRJNA237344SRR1792674river
amazon_river_0_2_2_0um_from_tapajos_surface PRJNA237345SRR1777513PRJNA237344SRR1796116river
amazon_river_2_0_297um_from_belem PRJNA237345SRR1781811PRJNA237344SRR1790644river
amazon_river_2_0_297um_from_macapa_north_channelPRJNA237345SRR1785350PRJNA237344SRR1786281river
amazon_river_2_0_297um_from_macapa_south_channelPRJNA237345SRR1784304PRJNA237344SRR1787943river
amazon_river_2_0_297um_from_obidos PRJNA237345SRR1782579PRJNA237344SRR1790678river
amazon_river_2_0_297um_from_tapajos_depth PRJNA237345SRR1781711PRJNA237344SRR1792852river
amazon_river_2_0_297um_from_tapajos_surface PRJNA237345SRR1778024PRJNA237344SRR1796118river
\n" + ], + "text/latex": [ + "A grouped\\_df: 23 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t amazon\\_plume\\_2\\_0um\\_from\\_station\\_10 & PRJNA237345 & SRR1193190 & PRJNA237344 & SRR1205250 & river\\\\\n", + "\t amazon\\_plume\\_2\\_0um\\_from\\_station\\_2 & PRJNA237345 & SRR1193177 & PRJNA237344 & SRR1182511 & river\\\\\n", + "\t amazon\\_plume\\_2\\_0um\\_from\\_station\\_23 & PRJNA237345 & SRR1193237 & PRJNA237344 & SRR1202089 & river\\\\\n", + "\t amazon\\_plume\\_2\\_0um\\_from\\_station\\_27 & PRJNA237345 & SRR1193629 & PRJNA237344 & SRR1183643 & river\\\\\n", + "\t amazon\\_plume\\_2\\_0um\\_from\\_station\\_3 & PRJNA237345 & SRR1193226 & PRJNA237344 & SRR1199272 & river\\\\\n", + "\t amazon\\_plume\\_0\\_2\\_2\\_0um\\_from\\_station\\_10 & PRJNA237345 & SRR1186930 & PRJNA237344 & SRR1199271 & river\\\\\n", + "\t amazon\\_plume\\_0\\_2\\_2\\_0um\\_from\\_station\\_2 & PRJNA237345 & SRR1193205 & PRJNA237344 & SRR1182512 & river\\\\\n", + "\t amazon\\_plume\\_0\\_2\\_2\\_0um\\_from\\_station\\_23 & PRJNA237345 & SRR1193632 & PRJNA237344 & SRR1186214 & river\\\\\n", + "\t amazon\\_plume\\_0\\_2\\_2\\_0um\\_from\\_station\\_25 & PRJNA237345 & SRR1204579 & PRJNA237344 & SRR1202090 & river\\\\\n", + "\t amazon\\_plume\\_0\\_2\\_2\\_0um\\_from\\_station\\_27 & PRJNA237345 & SRR1193627 & PRJNA237344 & SRR1183650 & river\\\\\n", + "\t amazon\\_plume\\_0\\_2\\_2\\_0um\\_from\\_station\\_3 & PRJNA237345 & SRR1193215 & PRJNA237344 & SRR1185413 & river\\\\\n", + "\t amazon\\_river\\_0\\_2\\_2\\_0um\\_from\\_belem & PRJNA237345 & SRR1781804 & PRJNA237344 & SRR1790489 & river\\\\\n", + "\t amazon\\_river\\_0\\_2\\_2\\_0um\\_from\\_macapa\\_north\\_channel & PRJNA237345 & SRR1785209 & PRJNA237344 & SRR1786279 & river\\\\\n", + "\t amazon\\_river\\_0\\_2\\_2\\_0um\\_from\\_macapa\\_south\\_channel & PRJNA237345 & SRR1784299 & PRJNA237344 & SRR1787940 & river\\\\\n", + "\t amazon\\_river\\_0\\_2\\_2\\_0um\\_from\\_obidos & PRJNA237345 & SRR1781945 & PRJNA237344 & SRR1790676 & river\\\\\n", + "\t amazon\\_river\\_0\\_2\\_2\\_0um\\_from\\_tapajos\\_depth & PRJNA237345 & SRR1779221 & PRJNA237344 & SRR1792674 & river\\\\\n", + "\t amazon\\_river\\_0\\_2\\_2\\_0um\\_from\\_tapajos\\_surface & PRJNA237345 & SRR1777513 & PRJNA237344 & SRR1796116 & river\\\\\n", + "\t amazon\\_river\\_2\\_0\\_297um\\_from\\_belem & PRJNA237345 & SRR1781811 & PRJNA237344 & SRR1790644 & river\\\\\n", + "\t amazon\\_river\\_2\\_0\\_297um\\_from\\_macapa\\_north\\_channel & PRJNA237345 & SRR1785350 & PRJNA237344 & SRR1786281 & river\\\\\n", + "\t amazon\\_river\\_2\\_0\\_297um\\_from\\_macapa\\_south\\_channel & PRJNA237345 & SRR1784304 & PRJNA237344 & SRR1787943 & river\\\\\n", + "\t amazon\\_river\\_2\\_0\\_297um\\_from\\_obidos & PRJNA237345 & SRR1782579 & PRJNA237344 & SRR1790678 & river\\\\\n", + "\t amazon\\_river\\_2\\_0\\_297um\\_from\\_tapajos\\_depth & PRJNA237345 & SRR1781711 & PRJNA237344 & SRR1792852 & river\\\\\n", + "\t amazon\\_river\\_2\\_0\\_297um\\_from\\_tapajos\\_surface & PRJNA237345 & SRR1778024 & PRJNA237344 & SRR1796118 & river\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A grouped_df: 23 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| amazon_plume_2_0um_from_station_10 | PRJNA237345 | SRR1193190 | PRJNA237344 | SRR1205250 | river |\n", + "| amazon_plume_2_0um_from_station_2 | PRJNA237345 | SRR1193177 | PRJNA237344 | SRR1182511 | river |\n", + "| amazon_plume_2_0um_from_station_23 | PRJNA237345 | SRR1193237 | PRJNA237344 | SRR1202089 | river |\n", + "| amazon_plume_2_0um_from_station_27 | PRJNA237345 | SRR1193629 | PRJNA237344 | SRR1183643 | river |\n", + "| amazon_plume_2_0um_from_station_3 | PRJNA237345 | SRR1193226 | PRJNA237344 | SRR1199272 | river |\n", + "| amazon_plume_0_2_2_0um_from_station_10 | PRJNA237345 | SRR1186930 | PRJNA237344 | SRR1199271 | river |\n", + "| amazon_plume_0_2_2_0um_from_station_2 | PRJNA237345 | SRR1193205 | PRJNA237344 | SRR1182512 | river |\n", + "| amazon_plume_0_2_2_0um_from_station_23 | PRJNA237345 | SRR1193632 | PRJNA237344 | SRR1186214 | river |\n", + "| amazon_plume_0_2_2_0um_from_station_25 | PRJNA237345 | SRR1204579 | PRJNA237344 | SRR1202090 | river |\n", + "| amazon_plume_0_2_2_0um_from_station_27 | PRJNA237345 | SRR1193627 | PRJNA237344 | SRR1183650 | river |\n", + "| amazon_plume_0_2_2_0um_from_station_3 | PRJNA237345 | SRR1193215 | PRJNA237344 | SRR1185413 | river |\n", + "| amazon_river_0_2_2_0um_from_belem | PRJNA237345 | SRR1781804 | PRJNA237344 | SRR1790489 | river |\n", + "| amazon_river_0_2_2_0um_from_macapa_north_channel | PRJNA237345 | SRR1785209 | PRJNA237344 | SRR1786279 | river |\n", + "| amazon_river_0_2_2_0um_from_macapa_south_channel | PRJNA237345 | SRR1784299 | PRJNA237344 | SRR1787940 | river |\n", + "| amazon_river_0_2_2_0um_from_obidos | PRJNA237345 | SRR1781945 | PRJNA237344 | SRR1790676 | river |\n", + "| amazon_river_0_2_2_0um_from_tapajos_depth | PRJNA237345 | SRR1779221 | PRJNA237344 | SRR1792674 | river |\n", + "| amazon_river_0_2_2_0um_from_tapajos_surface | PRJNA237345 | SRR1777513 | PRJNA237344 | SRR1796116 | river |\n", + "| amazon_river_2_0_297um_from_belem | PRJNA237345 | SRR1781811 | PRJNA237344 | SRR1790644 | river |\n", + "| amazon_river_2_0_297um_from_macapa_north_channel | PRJNA237345 | SRR1785350 | PRJNA237344 | SRR1786281 | river |\n", + "| amazon_river_2_0_297um_from_macapa_south_channel | PRJNA237345 | SRR1784304 | PRJNA237344 | SRR1787943 | river |\n", + "| amazon_river_2_0_297um_from_obidos | PRJNA237345 | SRR1782579 | PRJNA237344 | SRR1790678 | river |\n", + "| amazon_river_2_0_297um_from_tapajos_depth | PRJNA237345 | SRR1781711 | PRJNA237344 | SRR1792852 | river |\n", + "| amazon_river_2_0_297um_from_tapajos_surface | PRJNA237345 | SRR1778024 | PRJNA237344 | SRR1796118 | river |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession\n", + "1 amazon_plume_2_0um_from_station_10 PRJNA237345 \n", + "2 amazon_plume_2_0um_from_station_2 PRJNA237345 \n", + "3 amazon_plume_2_0um_from_station_23 PRJNA237345 \n", + "4 amazon_plume_2_0um_from_station_27 PRJNA237345 \n", + "5 amazon_plume_2_0um_from_station_3 PRJNA237345 \n", + "6 amazon_plume_0_2_2_0um_from_station_10 PRJNA237345 \n", + "7 amazon_plume_0_2_2_0um_from_station_2 PRJNA237345 \n", + "8 amazon_plume_0_2_2_0um_from_station_23 PRJNA237345 \n", + "9 amazon_plume_0_2_2_0um_from_station_25 PRJNA237345 \n", + "10 amazon_plume_0_2_2_0um_from_station_27 PRJNA237345 \n", + "11 amazon_plume_0_2_2_0um_from_station_3 PRJNA237345 \n", + "12 amazon_river_0_2_2_0um_from_belem PRJNA237345 \n", + "13 amazon_river_0_2_2_0um_from_macapa_north_channel PRJNA237345 \n", + "14 amazon_river_0_2_2_0um_from_macapa_south_channel PRJNA237345 \n", + "15 amazon_river_0_2_2_0um_from_obidos PRJNA237345 \n", + "16 amazon_river_0_2_2_0um_from_tapajos_depth PRJNA237345 \n", + "17 amazon_river_0_2_2_0um_from_tapajos_surface PRJNA237345 \n", + "18 amazon_river_2_0_297um_from_belem PRJNA237345 \n", + "19 amazon_river_2_0_297um_from_macapa_north_channel PRJNA237345 \n", + "20 amazon_river_2_0_297um_from_macapa_south_channel PRJNA237345 \n", + "21 amazon_river_2_0_297um_from_obidos PRJNA237345 \n", + "22 amazon_river_2_0_297um_from_tapajos_depth PRJNA237345 \n", + "23 amazon_river_2_0_297um_from_tapajos_surface PRJNA237345 \n", + " mtx_run_accession mgx_study_accession mgx_run_accession sample_type\n", + "1 SRR1193190 PRJNA237344 SRR1205250 river \n", + "2 SRR1193177 PRJNA237344 SRR1182511 river \n", + "3 SRR1193237 PRJNA237344 SRR1202089 river \n", + "4 SRR1193629 PRJNA237344 SRR1183643 river \n", + "5 SRR1193226 PRJNA237344 SRR1199272 river \n", + "6 SRR1186930 PRJNA237344 SRR1199271 river \n", + "7 SRR1193205 PRJNA237344 SRR1182512 river \n", + "8 SRR1193632 PRJNA237344 SRR1186214 river \n", + "9 SRR1204579 PRJNA237344 SRR1202090 river \n", + "10 SRR1193627 PRJNA237344 SRR1183650 river \n", + "11 SRR1193215 PRJNA237344 SRR1185413 river \n", + "12 SRR1781804 PRJNA237344 SRR1790489 river \n", + "13 SRR1785209 PRJNA237344 SRR1786279 river \n", + "14 SRR1784299 PRJNA237344 SRR1787940 river \n", + "15 SRR1781945 PRJNA237344 SRR1790676 river \n", + "16 SRR1779221 PRJNA237344 SRR1792674 river \n", + "17 SRR1777513 PRJNA237344 SRR1796116 river \n", + "18 SRR1781811 PRJNA237344 SRR1790644 river \n", + "19 SRR1785350 PRJNA237344 SRR1786281 river \n", + "20 SRR1784304 PRJNA237344 SRR1787943 river \n", + "21 SRR1782579 PRJNA237344 SRR1790678 river \n", + "22 SRR1781711 PRJNA237344 SRR1792852 river \n", + "23 SRR1778024 PRJNA237344 SRR1796118 river " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "tmp1 <- prjna237345 %>% \n", + " select(sample_name = experiment_title, mtx_study_accession = study_accession, mtx_run_accession = run_accession) %>%\n", + " mutate(sample_name = gsub(\"Metatranscriptome \", \"\", sample_name),\n", + " sample_name = gsub(\"\\\\(.*\", \"\", sample_name))\n", + "\n", + "tmp2 <- prjna237344 %>% \n", + " select(sample_name = experiment_title, mgx_study_accession = study_accession, mgx_run_accession = run_accession) %>%\n", + " mutate(sample_name = gsub(\"Metagenome \", \"\", sample_name),\n", + " sample_name = gsub(\"\\\\(.*\", \"\", sample_name))\n", + "\n", + "prjna237345_vs_prjna237344 <- left_join(tmp1, tmp2) %>%\n", + " filter(!is.na(mgx_study_accession)) %>%\n", + " mutate(sample_name = gsub(\"Illumina Genome Analyzer IIx sequencing; May-June 2010 \", \"\", sample_name),\n", + " sample_name = gsub(\"Illumina HiSeq 2500 sequencing; May 2011 \", \"\", sample_name)) %>%\n", + " group_by(sample_name) %>%\n", + " slice(n = 1) %>%\n", + " mutate(sample_name = make_clean_names(sample_name),\n", + " sample_type = \"river\")\n", + "\n", + "prjna237345_vs_prjna237344" + ] + }, + { + "cell_type": "markdown", + "id": "49c6b061", + "metadata": {}, + "source": [ + "## PRJNA453733" + ] + }, + { + "cell_type": "code", + "execution_count": 19, + "id": "f074e2aa", + "metadata": {}, + "outputs": [], + "source": [ + "prjna453733 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA453733&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F) %>%\n", + " filter(library_strategy == \"OTHER\")" + ] + }, + { + "cell_type": "code", + "execution_count": 20, + "id": "947b707d", + "metadata": { + "scrolled": true + }, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 5 × 5
study_accessionrun_accessionsample_aliaslibrary_strategylibrary_source
<chr><chr><chr><chr><chr>
PRJNA453733SRR7083928CS-Sed16-2cmAOTHERMETATRANSCRIPTOMIC
PRJNA453733SRR7083929CS-Sed16-2cmBOTHERMETAGENOMIC
PRJNA453733SRR7083930CS-Br16 OTHERMETAGENOMIC
PRJNA453733SRR7083931CS-Sed16-2cmAOTHERMETAGENOMIC
PRJNA453733SRR7083934CS-Sed16-5cm OTHERMETAGENOMIC
\n" + ], + "text/latex": [ + "A tibble: 5 × 5\n", + "\\begin{tabular}{lllll}\n", + " study\\_accession & run\\_accession & sample\\_alias & library\\_strategy & library\\_source\\\\\n", + " & & & & \\\\\n", + "\\hline\n", + "\t PRJNA453733 & SRR7083928 & CS-Sed16-2cmA & OTHER & METATRANSCRIPTOMIC\\\\\n", + "\t PRJNA453733 & SRR7083929 & CS-Sed16-2cmB & OTHER & METAGENOMIC \\\\\n", + "\t PRJNA453733 & SRR7083930 & CS-Br16 & OTHER & METAGENOMIC \\\\\n", + "\t PRJNA453733 & SRR7083931 & CS-Sed16-2cmA & OTHER & METAGENOMIC \\\\\n", + "\t PRJNA453733 & SRR7083934 & CS-Sed16-5cm & OTHER & METAGENOMIC \\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 5 × 5\n", + "\n", + "| study_accession <chr> | run_accession <chr> | sample_alias <chr> | library_strategy <chr> | library_source <chr> |\n", + "|---|---|---|---|---|\n", + "| PRJNA453733 | SRR7083928 | CS-Sed16-2cmA | OTHER | METATRANSCRIPTOMIC |\n", + "| PRJNA453733 | SRR7083929 | CS-Sed16-2cmB | OTHER | METAGENOMIC |\n", + "| PRJNA453733 | SRR7083930 | CS-Br16 | OTHER | METAGENOMIC |\n", + "| PRJNA453733 | SRR7083931 | CS-Sed16-2cmA | OTHER | METAGENOMIC |\n", + "| PRJNA453733 | SRR7083934 | CS-Sed16-5cm | OTHER | METAGENOMIC |\n", + "\n" + ], + "text/plain": [ + " study_accession run_accession sample_alias library_strategy\n", + "1 PRJNA453733 SRR7083928 CS-Sed16-2cmA OTHER \n", + "2 PRJNA453733 SRR7083929 CS-Sed16-2cmB OTHER \n", + "3 PRJNA453733 SRR7083930 CS-Br16 OTHER \n", + "4 PRJNA453733 SRR7083931 CS-Sed16-2cmA OTHER \n", + "5 PRJNA453733 SRR7083934 CS-Sed16-5cm OTHER \n", + " library_source \n", + "1 METATRANSCRIPTOMIC\n", + "2 METAGENOMIC \n", + "3 METAGENOMIC \n", + "4 METAGENOMIC \n", + "5 METAGENOMIC " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna453733 %>% \n", + " select(study_accession, run_accession, sample_alias, library_strategy, library_source)" + ] + }, + { + "cell_type": "code", + "execution_count": 21, + "id": "18b6e2a1", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\n", + "
A tibble: 1 × 2
sample_aliasn
<chr><int>
CS-Sed16-2cmA2
\n" + ], + "text/latex": [ + "A tibble: 1 × 2\n", + "\\begin{tabular}{ll}\n", + " sample\\_alias & n\\\\\n", + " & \\\\\n", + "\\hline\n", + "\t CS-Sed16-2cmA & 2\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 1 × 2\n", + "\n", + "| sample_alias <chr> | n <int> |\n", + "|---|---|\n", + "| CS-Sed16-2cmA | 2 |\n", + "\n" + ], + "text/plain": [ + " sample_alias n\n", + "1 CS-Sed16-2cmA 2" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna453733_pairs <- prjna453733 %>%\n", + " group_by(sample_alias) %>%\n", + " tally() %>%\n", + " filter(n == 2)\n", + "\n", + "prjna453733_pairs" + ] + }, + { + "cell_type": "code", + "execution_count": 22, + "id": "9016f1ab", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\n", + "
A tibble: 1 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
CS-Sed16-2cmAPRJNA453733SRR7083928PRJNA453733SRR7083931lake
\n" + ], + "text/latex": [ + "A tibble: 1 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t CS-Sed16-2cmA & PRJNA453733 & SRR7083928 & PRJNA453733 & SRR7083931 & lake\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 1 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| CS-Sed16-2cmA | PRJNA453733 | SRR7083928 | PRJNA453733 | SRR7083931 | lake |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 CS-Sed16-2cmA PRJNA453733 SRR7083928 PRJNA453733 \n", + " mgx_run_accession sample_type\n", + "1 SRR7083931 lake " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna453733_mtx <- prjna453733 %>%\n", + " filter(sample_alias %in% prjna453733_pairs$sample_alias) %>%\n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mtx_study_accession = study_accession, \n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna453733_mgx <- prjna453733 %>%\n", + " filter(sample_alias %in% prjna453733_pairs$sample_alias) %>%\n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession, \n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna453733_mtx_vs_mgx <- left_join(prjna453733_mtx, prjna453733_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"lake\")\n", + "prjna453733_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "7e95696f", + "metadata": {}, + "source": [ + "## Microbial metagenomes and metatranscriptomes during a coastal phytoplankton bloom (one PRJNA per sample)\n", + "The samples published in the above title each have there own study accession and run accession. \n", + "This section of the notebook parses two supplementary files from the publication to get all of the sample bioproject numbers and to determine which bioproject numbers represent pairs of samples." + ] + }, + { + "cell_type": "code", + "execution_count": 23, + "id": "eee996ab", + "metadata": {}, + "outputs": [], + "source": [ + "download.file(url = \"https://static-content.springer.com/esm/art%3A10.1038%2Fs41597-019-0132-4/MediaObjects/41597_2019_132_MOESM1_ESM.zip\",\n", + " destfile = \"inputs/metadata/41597_2019_132_MOESM1_ESM.zip\")" + ] + }, + { + "cell_type": "code", + "execution_count": 24, + "id": "89777aca", + "metadata": {}, + "outputs": [], + "source": [ + "unzip(\"inputs/metadata/41597_2019_132_MOESM1_ESM.zip\", exdir = \"inputs/metadata/41597_2019_132_MOESM1_ESM\")" + ] + }, + { + "cell_type": "code", + "execution_count": 25, + "id": "102b69b1", + "metadata": {}, + "outputs": [], + "source": [ + "moran010B_mgx <- read_tsv(\"inputs/metadata/41597_2019_132_MOESM1_ESM/a_Moran010B_dna.txt\", show_col_types = F) %>%\n", + " clean_names() %>%\n", + " select(sample_name, mgx_study_accession = assay_name) %>%\n", + " mutate(sample_name = gsub(\"D\", \"\", sample_name))\n", + "\n", + "moran010B_mtx <- read_tsv(\"inputs/metadata/41597_2019_132_MOESM1_ESM/a_Moran010B_rna.txt\", show_col_types = F) %>%\n", + " clean_names() %>%\n", + " select(sample_name, mtx_study_accession = assay_name) %>%\n", + " mutate(sample_name = gsub(\"R\", \"\", sample_name))\n", + "\n", + "moran010B <- inner_join(moran010B_mtx, moran010B_mgx, by = \"sample_name\") " + ] + }, + { + "cell_type": "code", + "execution_count": 26, + "id": "bbc1cf54", + "metadata": {}, + "outputs": [], + "source": [ + "# use the moran010B study accessions to programatically generate URLs to get run accessions for each sample\n", + "moran010B_run_accessions <- data.frame()\n", + "for(study_accession in c(moran010B$mtx_study_accession, moran010B_mgx$mgx_study_accession)){\n", + " url <- paste0(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=\", study_accession, \"&result=read_run&fields=study_accession,run_accession&format=tsv&download=true&limit=0\")\n", + " run_accession <- read_tsv(url, show_col_types = F) \n", + " moran010B_run_accessions <- bind_rows(moran010B_run_accessions, run_accession)\n", + "}" + ] + }, + { + "cell_type": "code", + "execution_count": 27, + "id": "5207828e", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 74 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
26 PRJNA502453SRR8361532PRJNA467728SRR7592711ocean
58_r PRJNA502454SRR8361534PRJNA467772SRR7609573ocean
125_rPRJNA468332SRR7962480PRJNA467773SRR7609569ocean
8 PRJNA502455SRR8297879PRJNA467724SRR7592287ocean
85 PRJNA502456SRR8297845PRJNA467765SRR7609362ocean
76 PRJNA468305SRR7949679PRJNA467757SRR7608731ocean
40 PRJNA467774SRR7609608PRJNA467736SRR7595425ocean
53 PRJNA468299SRR7609574PRJNA502421SRR8361352ocean
73 PRJNA468306SRR7949683PRJNA467754SRR7608223ocean
23 PRJNA467775SRR7609632PRJNA467727SRR7633009ocean
49 PRJNA467776SRR7609653PRJNA502442SRR8361104ocean
14 PRJNA468308SRR7949682PRJNA502410SRR8360542ocean
44 PRJNA502457SRR8361579PRJNA467738SRR7600393ocean
29 PRJNA468309SRR7949681PRJNA467730SRR7593831ocean
18 PRJNA467777SRR7609655PRJNA502413SRR8360544ocean
24 PRJNA502458SRR8361603PRJNA502440SRR8360549ocean
79 PRJNA468143SRR7609654PRJNA467760SRR7609143ocean
22 PRJNA468300SRR7609599PRJNA502414SRR8360545ocean
21 PRJNA468310SRR7949678PRJNA468208SRR7592488ocean
60 PRJNA467778SRR7609828PRJNA502425SRR8361517ocean
15 PRJNA468144SRR7609827PRJNA502411SRR8360457ocean
75 PRJNA502459SRR8361602PRJNA467756SRR7608423ocean
3 PRJNA468311SRR7949692PRJNA467722SRR7592226ocean
20 PRJNA467779SRR7609829PRJNA467726SRR7592489ocean
91 PRJNA468312SRR7949677PRJNA467769SRR7609461ocean
56 PRJNA468152SRR7615220PRJNA467743SRR7601261ocean
46 PRJNA502460SRR8297843PRJNA468213SRR7600395ocean
2 PRJNA468313SRR7609607PRJNA467721SRR7592225ocean
38 PRJNA468301SRR7609600PRJNA468211SRR7594461ocean
45 PRJNA468314SRR7962234PRJNA467739SRR7600394ocean
32 PRJNA468321SRR7962267PRJNA468210SRR7594064ocean
81 PRJNA468145SRR7610454PRJNA467762SRR7609163ocean
70 PRJNA468322SRR7962239PRJNA467752SRR7608010ocean
67 PRJNA468323SRR7962329PRJNA467750SRR7607757ocean
33 PRJNA467783SRR7610453PRJNA502417SRR8361102ocean
27 PRJNA468331SRR7962479PRJNA468209SRR7593830ocean
34 PRJNA502465SRR8297868PRJNA467732SRR7594085ocean
4 PRJNA468146SRR7610452PRJNA467723SRR7592236ocean
57 PRJNA468147SRR7610935PRJNA502423SRR8361526ocean
1 PRJNA502466SRR8361608PRJNA467720SRR7592212ocean
47 PRJNA468148SRR7610853PRJNA467740SRR7600454ocean
9 PRJNA502467SRR8297869PRJNA502408SRR8360547ocean
77 PRJNA468324SRR7962474PRJNA467758SRR7608832ocean
55 PRJNA467784SRR7610867PRJNA502422SRR8361371ocean
10 PRJNA468325SRR7962473PRJNA502409SRR8360543ocean
11 PRJNA468326SRR7962475PRJNA467725SRR7592321ocean
58 PRJNA502468SRR8297878PRJNA502424SRR8361527ocean
52 PRJNA468327SRR7962478PRJNA467742SRR7600455ocean
51 PRJNA468149SRR7611057PRJNA502420SRR8361350ocean
50 PRJNA468328SRR7962477PRJNA502419SRR8361349ocean
90 PRJNA467785SRR7611056PRJNA467768SRR7609452ocean
80 PRJNA467786SRR7611058PRJNA467761SRR7609166ocean
89 PRJNA468150SRR7611500PRJNA467766SRR7609363ocean
35 PRJNA467787SRR7612580PRJNA502441SRR8361101ocean
71 PRJNA468329SRR7962476PRJNA467753SRR7608017ocean
31 PRJNA468151SRR7611499PRJNA467731SRR7593934ocean
84 PRJNA502451SRR8393198PRJNA467764SRR7609361ocean
61 PRJNA467788SRR7615219PRJNA502426SRR8361516ocean
36_rPRJNA468330SRR7962481PRJNA467770SRR7609462ocean
82 PRJNA502452SRR8361531PRJNA467763SRR7609344ocean
\n" + ], + "text/latex": [ + "A tibble: 74 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t 26 & PRJNA502453 & SRR8361532 & PRJNA467728 & SRR7592711 & ocean\\\\\n", + "\t 58\\_r & PRJNA502454 & SRR8361534 & PRJNA467772 & SRR7609573 & ocean\\\\\n", + "\t 125\\_r & PRJNA468332 & SRR7962480 & PRJNA467773 & SRR7609569 & ocean\\\\\n", + "\t 8 & PRJNA502455 & SRR8297879 & PRJNA467724 & SRR7592287 & ocean\\\\\n", + "\t 85 & PRJNA502456 & SRR8297845 & PRJNA467765 & SRR7609362 & ocean\\\\\n", + "\t 76 & PRJNA468305 & SRR7949679 & PRJNA467757 & SRR7608731 & ocean\\\\\n", + "\t 40 & PRJNA467774 & SRR7609608 & PRJNA467736 & SRR7595425 & ocean\\\\\n", + "\t 53 & PRJNA468299 & SRR7609574 & PRJNA502421 & SRR8361352 & ocean\\\\\n", + "\t 73 & PRJNA468306 & SRR7949683 & PRJNA467754 & SRR7608223 & ocean\\\\\n", + "\t 23 & PRJNA467775 & SRR7609632 & PRJNA467727 & SRR7633009 & ocean\\\\\n", + "\t 49 & PRJNA467776 & SRR7609653 & PRJNA502442 & SRR8361104 & ocean\\\\\n", + "\t 14 & PRJNA468308 & SRR7949682 & PRJNA502410 & SRR8360542 & ocean\\\\\n", + "\t 44 & PRJNA502457 & SRR8361579 & PRJNA467738 & SRR7600393 & ocean\\\\\n", + "\t 29 & PRJNA468309 & SRR7949681 & PRJNA467730 & SRR7593831 & ocean\\\\\n", + "\t 18 & PRJNA467777 & SRR7609655 & PRJNA502413 & SRR8360544 & ocean\\\\\n", + "\t 24 & PRJNA502458 & SRR8361603 & PRJNA502440 & SRR8360549 & ocean\\\\\n", + "\t 79 & PRJNA468143 & SRR7609654 & PRJNA467760 & SRR7609143 & ocean\\\\\n", + "\t 22 & PRJNA468300 & SRR7609599 & PRJNA502414 & SRR8360545 & ocean\\\\\n", + "\t 21 & PRJNA468310 & SRR7949678 & PRJNA468208 & SRR7592488 & ocean\\\\\n", + "\t 60 & PRJNA467778 & SRR7609828 & PRJNA502425 & SRR8361517 & ocean\\\\\n", + "\t 15 & PRJNA468144 & SRR7609827 & PRJNA502411 & SRR8360457 & ocean\\\\\n", + "\t 75 & PRJNA502459 & SRR8361602 & PRJNA467756 & SRR7608423 & ocean\\\\\n", + "\t 3 & PRJNA468311 & SRR7949692 & PRJNA467722 & SRR7592226 & ocean\\\\\n", + "\t 20 & PRJNA467779 & SRR7609829 & PRJNA467726 & SRR7592489 & ocean\\\\\n", + "\t 91 & PRJNA468312 & SRR7949677 & PRJNA467769 & SRR7609461 & ocean\\\\\n", + "\t 56 & PRJNA468152 & SRR7615220 & PRJNA467743 & SRR7601261 & ocean\\\\\n", + "\t 46 & PRJNA502460 & SRR8297843 & PRJNA468213 & SRR7600395 & ocean\\\\\n", + "\t 2 & PRJNA468313 & SRR7609607 & PRJNA467721 & SRR7592225 & ocean\\\\\n", + "\t 38 & PRJNA468301 & SRR7609600 & PRJNA468211 & SRR7594461 & ocean\\\\\n", + "\t 45 & PRJNA468314 & SRR7962234 & PRJNA467739 & SRR7600394 & ocean\\\\\n", + "\t ⋮ & ⋮ & ⋮ & ⋮ & ⋮ & ⋮\\\\\n", + "\t 32 & PRJNA468321 & SRR7962267 & PRJNA468210 & SRR7594064 & ocean\\\\\n", + "\t 81 & PRJNA468145 & SRR7610454 & PRJNA467762 & SRR7609163 & ocean\\\\\n", + "\t 70 & PRJNA468322 & SRR7962239 & PRJNA467752 & SRR7608010 & ocean\\\\\n", + "\t 67 & PRJNA468323 & SRR7962329 & PRJNA467750 & SRR7607757 & ocean\\\\\n", + "\t 33 & PRJNA467783 & SRR7610453 & PRJNA502417 & SRR8361102 & ocean\\\\\n", + "\t 27 & PRJNA468331 & SRR7962479 & PRJNA468209 & SRR7593830 & ocean\\\\\n", + "\t 34 & PRJNA502465 & SRR8297868 & PRJNA467732 & SRR7594085 & ocean\\\\\n", + "\t 4 & PRJNA468146 & SRR7610452 & PRJNA467723 & SRR7592236 & ocean\\\\\n", + "\t 57 & PRJNA468147 & SRR7610935 & PRJNA502423 & SRR8361526 & ocean\\\\\n", + "\t 1 & PRJNA502466 & SRR8361608 & PRJNA467720 & SRR7592212 & ocean\\\\\n", + "\t 47 & PRJNA468148 & SRR7610853 & PRJNA467740 & SRR7600454 & ocean\\\\\n", + "\t 9 & PRJNA502467 & SRR8297869 & PRJNA502408 & SRR8360547 & ocean\\\\\n", + "\t 77 & PRJNA468324 & SRR7962474 & PRJNA467758 & SRR7608832 & ocean\\\\\n", + "\t 55 & PRJNA467784 & SRR7610867 & PRJNA502422 & SRR8361371 & ocean\\\\\n", + "\t 10 & PRJNA468325 & SRR7962473 & PRJNA502409 & SRR8360543 & ocean\\\\\n", + "\t 11 & PRJNA468326 & SRR7962475 & PRJNA467725 & SRR7592321 & ocean\\\\\n", + "\t 58 & PRJNA502468 & SRR8297878 & PRJNA502424 & SRR8361527 & ocean\\\\\n", + "\t 52 & PRJNA468327 & SRR7962478 & PRJNA467742 & SRR7600455 & ocean\\\\\n", + "\t 51 & PRJNA468149 & SRR7611057 & PRJNA502420 & SRR8361350 & ocean\\\\\n", + "\t 50 & PRJNA468328 & SRR7962477 & PRJNA502419 & SRR8361349 & ocean\\\\\n", + "\t 90 & PRJNA467785 & SRR7611056 & PRJNA467768 & SRR7609452 & ocean\\\\\n", + "\t 80 & PRJNA467786 & SRR7611058 & PRJNA467761 & SRR7609166 & ocean\\\\\n", + "\t 89 & PRJNA468150 & SRR7611500 & PRJNA467766 & SRR7609363 & ocean\\\\\n", + "\t 35 & PRJNA467787 & SRR7612580 & PRJNA502441 & SRR8361101 & ocean\\\\\n", + "\t 71 & PRJNA468329 & SRR7962476 & PRJNA467753 & SRR7608017 & ocean\\\\\n", + "\t 31 & PRJNA468151 & SRR7611499 & PRJNA467731 & SRR7593934 & ocean\\\\\n", + "\t 84 & PRJNA502451 & SRR8393198 & PRJNA467764 & SRR7609361 & ocean\\\\\n", + "\t 61 & PRJNA467788 & SRR7615219 & PRJNA502426 & SRR8361516 & ocean\\\\\n", + "\t 36\\_r & PRJNA468330 & SRR7962481 & PRJNA467770 & SRR7609462 & ocean\\\\\n", + "\t 82 & PRJNA502452 & SRR8361531 & PRJNA467763 & SRR7609344 & ocean\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 74 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| 26 | PRJNA502453 | SRR8361532 | PRJNA467728 | SRR7592711 | ocean |\n", + "| 58_r | PRJNA502454 | SRR8361534 | PRJNA467772 | SRR7609573 | ocean |\n", + "| 125_r | PRJNA468332 | SRR7962480 | PRJNA467773 | SRR7609569 | ocean |\n", + "| 8 | PRJNA502455 | SRR8297879 | PRJNA467724 | SRR7592287 | ocean |\n", + "| 85 | PRJNA502456 | SRR8297845 | PRJNA467765 | SRR7609362 | ocean |\n", + "| 76 | PRJNA468305 | SRR7949679 | PRJNA467757 | SRR7608731 | ocean |\n", + "| 40 | PRJNA467774 | SRR7609608 | PRJNA467736 | SRR7595425 | ocean |\n", + "| 53 | PRJNA468299 | SRR7609574 | PRJNA502421 | SRR8361352 | ocean |\n", + "| 73 | PRJNA468306 | SRR7949683 | PRJNA467754 | SRR7608223 | ocean |\n", + "| 23 | PRJNA467775 | SRR7609632 | PRJNA467727 | SRR7633009 | ocean |\n", + "| 49 | PRJNA467776 | SRR7609653 | PRJNA502442 | SRR8361104 | ocean |\n", + "| 14 | PRJNA468308 | SRR7949682 | PRJNA502410 | SRR8360542 | ocean |\n", + "| 44 | PRJNA502457 | SRR8361579 | PRJNA467738 | SRR7600393 | ocean |\n", + "| 29 | PRJNA468309 | SRR7949681 | PRJNA467730 | SRR7593831 | ocean |\n", + "| 18 | PRJNA467777 | SRR7609655 | PRJNA502413 | SRR8360544 | ocean |\n", + "| 24 | PRJNA502458 | SRR8361603 | PRJNA502440 | SRR8360549 | ocean |\n", + "| 79 | PRJNA468143 | SRR7609654 | PRJNA467760 | SRR7609143 | ocean |\n", + "| 22 | PRJNA468300 | SRR7609599 | PRJNA502414 | SRR8360545 | ocean |\n", + "| 21 | PRJNA468310 | SRR7949678 | PRJNA468208 | SRR7592488 | ocean |\n", + "| 60 | PRJNA467778 | SRR7609828 | PRJNA502425 | SRR8361517 | ocean |\n", + "| 15 | PRJNA468144 | SRR7609827 | PRJNA502411 | SRR8360457 | ocean |\n", + "| 75 | PRJNA502459 | SRR8361602 | PRJNA467756 | SRR7608423 | ocean |\n", + "| 3 | PRJNA468311 | SRR7949692 | PRJNA467722 | SRR7592226 | ocean |\n", + "| 20 | PRJNA467779 | SRR7609829 | PRJNA467726 | SRR7592489 | ocean |\n", + "| 91 | PRJNA468312 | SRR7949677 | PRJNA467769 | SRR7609461 | ocean |\n", + "| 56 | PRJNA468152 | SRR7615220 | PRJNA467743 | SRR7601261 | ocean |\n", + "| 46 | PRJNA502460 | SRR8297843 | PRJNA468213 | SRR7600395 | ocean |\n", + "| 2 | PRJNA468313 | SRR7609607 | PRJNA467721 | SRR7592225 | ocean |\n", + "| 38 | PRJNA468301 | SRR7609600 | PRJNA468211 | SRR7594461 | ocean |\n", + "| 45 | PRJNA468314 | SRR7962234 | PRJNA467739 | SRR7600394 | ocean |\n", + "| ⋮ | ⋮ | ⋮ | ⋮ | ⋮ | ⋮ |\n", + "| 32 | PRJNA468321 | SRR7962267 | PRJNA468210 | SRR7594064 | ocean |\n", + "| 81 | PRJNA468145 | SRR7610454 | PRJNA467762 | SRR7609163 | ocean |\n", + "| 70 | PRJNA468322 | SRR7962239 | PRJNA467752 | SRR7608010 | ocean |\n", + "| 67 | PRJNA468323 | SRR7962329 | PRJNA467750 | SRR7607757 | ocean |\n", + "| 33 | PRJNA467783 | SRR7610453 | PRJNA502417 | SRR8361102 | ocean |\n", + "| 27 | PRJNA468331 | SRR7962479 | PRJNA468209 | SRR7593830 | ocean |\n", + "| 34 | PRJNA502465 | SRR8297868 | PRJNA467732 | SRR7594085 | ocean |\n", + "| 4 | PRJNA468146 | SRR7610452 | PRJNA467723 | SRR7592236 | ocean |\n", + "| 57 | PRJNA468147 | SRR7610935 | PRJNA502423 | SRR8361526 | ocean |\n", + "| 1 | PRJNA502466 | SRR8361608 | PRJNA467720 | SRR7592212 | ocean |\n", + "| 47 | PRJNA468148 | SRR7610853 | PRJNA467740 | SRR7600454 | ocean |\n", + "| 9 | PRJNA502467 | SRR8297869 | PRJNA502408 | SRR8360547 | ocean |\n", + "| 77 | PRJNA468324 | SRR7962474 | PRJNA467758 | SRR7608832 | ocean |\n", + "| 55 | PRJNA467784 | SRR7610867 | PRJNA502422 | SRR8361371 | ocean |\n", + "| 10 | PRJNA468325 | SRR7962473 | PRJNA502409 | SRR8360543 | ocean |\n", + "| 11 | PRJNA468326 | SRR7962475 | PRJNA467725 | SRR7592321 | ocean |\n", + "| 58 | PRJNA502468 | SRR8297878 | PRJNA502424 | SRR8361527 | ocean |\n", + "| 52 | PRJNA468327 | SRR7962478 | PRJNA467742 | SRR7600455 | ocean |\n", + "| 51 | PRJNA468149 | SRR7611057 | PRJNA502420 | SRR8361350 | ocean |\n", + "| 50 | PRJNA468328 | SRR7962477 | PRJNA502419 | SRR8361349 | ocean |\n", + "| 90 | PRJNA467785 | SRR7611056 | PRJNA467768 | SRR7609452 | ocean |\n", + "| 80 | PRJNA467786 | SRR7611058 | PRJNA467761 | SRR7609166 | ocean |\n", + "| 89 | PRJNA468150 | SRR7611500 | PRJNA467766 | SRR7609363 | ocean |\n", + "| 35 | PRJNA467787 | SRR7612580 | PRJNA502441 | SRR8361101 | ocean |\n", + "| 71 | PRJNA468329 | SRR7962476 | PRJNA467753 | SRR7608017 | ocean |\n", + "| 31 | PRJNA468151 | SRR7611499 | PRJNA467731 | SRR7593934 | ocean |\n", + "| 84 | PRJNA502451 | SRR8393198 | PRJNA467764 | SRR7609361 | ocean |\n", + "| 61 | PRJNA467788 | SRR7615219 | PRJNA502426 | SRR8361516 | ocean |\n", + "| 36_r | PRJNA468330 | SRR7962481 | PRJNA467770 | SRR7609462 | ocean |\n", + "| 82 | PRJNA502452 | SRR8361531 | PRJNA467763 | SRR7609344 | ocean |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 26 PRJNA502453 SRR8361532 PRJNA467728 \n", + "2 58_r PRJNA502454 SRR8361534 PRJNA467772 \n", + "3 125_r PRJNA468332 SRR7962480 PRJNA467773 \n", + "4 8 PRJNA502455 SRR8297879 PRJNA467724 \n", + "5 85 PRJNA502456 SRR8297845 PRJNA467765 \n", + "6 76 PRJNA468305 SRR7949679 PRJNA467757 \n", + "7 40 PRJNA467774 SRR7609608 PRJNA467736 \n", + "8 53 PRJNA468299 SRR7609574 PRJNA502421 \n", + "9 73 PRJNA468306 SRR7949683 PRJNA467754 \n", + "10 23 PRJNA467775 SRR7609632 PRJNA467727 \n", + "11 49 PRJNA467776 SRR7609653 PRJNA502442 \n", + "12 14 PRJNA468308 SRR7949682 PRJNA502410 \n", + "13 44 PRJNA502457 SRR8361579 PRJNA467738 \n", + "14 29 PRJNA468309 SRR7949681 PRJNA467730 \n", + "15 18 PRJNA467777 SRR7609655 PRJNA502413 \n", + "16 24 PRJNA502458 SRR8361603 PRJNA502440 \n", + "17 79 PRJNA468143 SRR7609654 PRJNA467760 \n", + "18 22 PRJNA468300 SRR7609599 PRJNA502414 \n", + "19 21 PRJNA468310 SRR7949678 PRJNA468208 \n", + "20 60 PRJNA467778 SRR7609828 PRJNA502425 \n", + "21 15 PRJNA468144 SRR7609827 PRJNA502411 \n", + "22 75 PRJNA502459 SRR8361602 PRJNA467756 \n", + "23 3 PRJNA468311 SRR7949692 PRJNA467722 \n", + "24 20 PRJNA467779 SRR7609829 PRJNA467726 \n", + "25 91 PRJNA468312 SRR7949677 PRJNA467769 \n", + "26 56 PRJNA468152 SRR7615220 PRJNA467743 \n", + "27 46 PRJNA502460 SRR8297843 PRJNA468213 \n", + "28 2 PRJNA468313 SRR7609607 PRJNA467721 \n", + "29 38 PRJNA468301 SRR7609600 PRJNA468211 \n", + "30 45 PRJNA468314 SRR7962234 PRJNA467739 \n", + "⋮ ⋮ ⋮ ⋮ ⋮ \n", + "45 32 PRJNA468321 SRR7962267 PRJNA468210 \n", + "46 81 PRJNA468145 SRR7610454 PRJNA467762 \n", + "47 70 PRJNA468322 SRR7962239 PRJNA467752 \n", + "48 67 PRJNA468323 SRR7962329 PRJNA467750 \n", + "49 33 PRJNA467783 SRR7610453 PRJNA502417 \n", + "50 27 PRJNA468331 SRR7962479 PRJNA468209 \n", + "51 34 PRJNA502465 SRR8297868 PRJNA467732 \n", + "52 4 PRJNA468146 SRR7610452 PRJNA467723 \n", + "53 57 PRJNA468147 SRR7610935 PRJNA502423 \n", + "54 1 PRJNA502466 SRR8361608 PRJNA467720 \n", + "55 47 PRJNA468148 SRR7610853 PRJNA467740 \n", + "56 9 PRJNA502467 SRR8297869 PRJNA502408 \n", + "57 77 PRJNA468324 SRR7962474 PRJNA467758 \n", + "58 55 PRJNA467784 SRR7610867 PRJNA502422 \n", + "59 10 PRJNA468325 SRR7962473 PRJNA502409 \n", + "60 11 PRJNA468326 SRR7962475 PRJNA467725 \n", + "61 58 PRJNA502468 SRR8297878 PRJNA502424 \n", + "62 52 PRJNA468327 SRR7962478 PRJNA467742 \n", + "63 51 PRJNA468149 SRR7611057 PRJNA502420 \n", + "64 50 PRJNA468328 SRR7962477 PRJNA502419 \n", + "65 90 PRJNA467785 SRR7611056 PRJNA467768 \n", + "66 80 PRJNA467786 SRR7611058 PRJNA467761 \n", + "67 89 PRJNA468150 SRR7611500 PRJNA467766 \n", + "68 35 PRJNA467787 SRR7612580 PRJNA502441 \n", + "69 71 PRJNA468329 SRR7962476 PRJNA467753 \n", + "70 31 PRJNA468151 SRR7611499 PRJNA467731 \n", + "71 84 PRJNA502451 SRR8393198 PRJNA467764 \n", + "72 61 PRJNA467788 SRR7615219 PRJNA502426 \n", + "73 36_r PRJNA468330 SRR7962481 PRJNA467770 \n", + "74 82 PRJNA502452 SRR8361531 PRJNA467763 \n", + " mgx_run_accession sample_type\n", + "1 SRR7592711 ocean \n", + "2 SRR7609573 ocean \n", + "3 SRR7609569 ocean \n", + "4 SRR7592287 ocean \n", + "5 SRR7609362 ocean \n", + "6 SRR7608731 ocean \n", + "7 SRR7595425 ocean \n", + "8 SRR8361352 ocean \n", + "9 SRR7608223 ocean \n", + "10 SRR7633009 ocean \n", + "11 SRR8361104 ocean \n", + "12 SRR8360542 ocean \n", + "13 SRR7600393 ocean \n", + "14 SRR7593831 ocean \n", + "15 SRR8360544 ocean \n", + "16 SRR8360549 ocean \n", + "17 SRR7609143 ocean \n", + "18 SRR8360545 ocean \n", + "19 SRR7592488 ocean \n", + "20 SRR8361517 ocean \n", + "21 SRR8360457 ocean \n", + "22 SRR7608423 ocean \n", + "23 SRR7592226 ocean \n", + "24 SRR7592489 ocean \n", + "25 SRR7609461 ocean \n", + "26 SRR7601261 ocean \n", + "27 SRR7600395 ocean \n", + "28 SRR7592225 ocean \n", + "29 SRR7594461 ocean \n", + "30 SRR7600394 ocean \n", + "⋮ ⋮ ⋮ \n", + "45 SRR7594064 ocean \n", + "46 SRR7609163 ocean \n", + "47 SRR7608010 ocean \n", + "48 SRR7607757 ocean \n", + "49 SRR8361102 ocean \n", + "50 SRR7593830 ocean \n", + "51 SRR7594085 ocean \n", + "52 SRR7592236 ocean \n", + "53 SRR8361526 ocean \n", + "54 SRR7592212 ocean \n", + "55 SRR7600454 ocean \n", + "56 SRR8360547 ocean \n", + "57 SRR7608832 ocean \n", + "58 SRR8361371 ocean \n", + "59 SRR8360543 ocean \n", + "60 SRR7592321 ocean \n", + "61 SRR8361527 ocean \n", + "62 SRR7600455 ocean \n", + "63 SRR8361350 ocean \n", + "64 SRR8361349 ocean \n", + "65 SRR7609452 ocean \n", + "66 SRR7609166 ocean \n", + "67 SRR7609363 ocean \n", + "68 SRR8361101 ocean \n", + "69 SRR7608017 ocean \n", + "70 SRR7593934 ocean \n", + "71 SRR7609361 ocean \n", + "72 SRR8361516 ocean \n", + "73 SRR7609462 ocean \n", + "74 SRR7609344 ocean " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "moran010B_mtx_vs_mgx <- moran010B %>%\n", + " left_join(moran010B_run_accessions, by = c(\"mtx_study_accession\" = \"study_accession\")) %>%\n", + " select(sample_name, mtx_study_accession, mtx_run_accession = run_accession, mgx_study_accession) %>%\n", + " left_join(moran010B_run_accessions, by = c(\"mgx_study_accession\" = \"study_accession\")) %>%\n", + " select(sample_name, mtx_study_accession, mtx_run_accession, mgx_study_accession, mgx_run_accession = run_accession) %>%\n", + " mutate(sample_type = \"ocean\")\n", + "\n", + "moran010B_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "2261971e", + "metadata": {}, + "source": [ + "## PRJNA603240" + ] + }, + { + "cell_type": "code", + "execution_count": 28, + "id": "1c8d6131", + "metadata": {}, + "outputs": [], + "source": [ + "prjna603240 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA603240&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 29, + "id": "155a92e3", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 10 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
sample_106PRJNA603240SRR10968230PRJNA603240SRR10968263deadwood
sample_069PRJNA603240SRR10968231PRJNA603240SRR10968264deadwood
sample_055PRJNA603240SRR10968232PRJNA603240SRR10968265deadwood
sample_049PRJNA603240SRR10968233PRJNA603240SRR10968266deadwood
sample_031PRJNA603240SRR10968234PRJNA603240SRR10968267deadwood
sample_116PRJNA603240SRR10968236PRJNA603240SRR10968225deadwood
sample_110PRJNA603240SRR10968237PRJNA603240SRR10968226deadwood
sample_044PRJNA603240SRR10968238PRJNA603240SRR10968227deadwood
sample_007PRJNA603240SRR10968239PRJNA603240SRR10968228deadwood
sample_006PRJNA603240SRR10968240PRJNA603240SRR10968229deadwood
\n" + ], + "text/latex": [ + "A tibble: 10 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t sample\\_106 & PRJNA603240 & SRR10968230 & PRJNA603240 & SRR10968263 & deadwood\\\\\n", + "\t sample\\_069 & PRJNA603240 & SRR10968231 & PRJNA603240 & SRR10968264 & deadwood\\\\\n", + "\t sample\\_055 & PRJNA603240 & SRR10968232 & PRJNA603240 & SRR10968265 & deadwood\\\\\n", + "\t sample\\_049 & PRJNA603240 & SRR10968233 & PRJNA603240 & SRR10968266 & deadwood\\\\\n", + "\t sample\\_031 & PRJNA603240 & SRR10968234 & PRJNA603240 & SRR10968267 & deadwood\\\\\n", + "\t sample\\_116 & PRJNA603240 & SRR10968236 & PRJNA603240 & SRR10968225 & deadwood\\\\\n", + "\t sample\\_110 & PRJNA603240 & SRR10968237 & PRJNA603240 & SRR10968226 & deadwood\\\\\n", + "\t sample\\_044 & PRJNA603240 & SRR10968238 & PRJNA603240 & SRR10968227 & deadwood\\\\\n", + "\t sample\\_007 & PRJNA603240 & SRR10968239 & PRJNA603240 & SRR10968228 & deadwood\\\\\n", + "\t sample\\_006 & PRJNA603240 & SRR10968240 & PRJNA603240 & SRR10968229 & deadwood\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 10 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| sample_106 | PRJNA603240 | SRR10968230 | PRJNA603240 | SRR10968263 | deadwood |\n", + "| sample_069 | PRJNA603240 | SRR10968231 | PRJNA603240 | SRR10968264 | deadwood |\n", + "| sample_055 | PRJNA603240 | SRR10968232 | PRJNA603240 | SRR10968265 | deadwood |\n", + "| sample_049 | PRJNA603240 | SRR10968233 | PRJNA603240 | SRR10968266 | deadwood |\n", + "| sample_031 | PRJNA603240 | SRR10968234 | PRJNA603240 | SRR10968267 | deadwood |\n", + "| sample_116 | PRJNA603240 | SRR10968236 | PRJNA603240 | SRR10968225 | deadwood |\n", + "| sample_110 | PRJNA603240 | SRR10968237 | PRJNA603240 | SRR10968226 | deadwood |\n", + "| sample_044 | PRJNA603240 | SRR10968238 | PRJNA603240 | SRR10968227 | deadwood |\n", + "| sample_007 | PRJNA603240 | SRR10968239 | PRJNA603240 | SRR10968228 | deadwood |\n", + "| sample_006 | PRJNA603240 | SRR10968240 | PRJNA603240 | SRR10968229 | deadwood |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 sample_106 PRJNA603240 SRR10968230 PRJNA603240 \n", + "2 sample_069 PRJNA603240 SRR10968231 PRJNA603240 \n", + "3 sample_055 PRJNA603240 SRR10968232 PRJNA603240 \n", + "4 sample_049 PRJNA603240 SRR10968233 PRJNA603240 \n", + "5 sample_031 PRJNA603240 SRR10968234 PRJNA603240 \n", + "6 sample_116 PRJNA603240 SRR10968236 PRJNA603240 \n", + "7 sample_110 PRJNA603240 SRR10968237 PRJNA603240 \n", + "8 sample_044 PRJNA603240 SRR10968238 PRJNA603240 \n", + "9 sample_007 PRJNA603240 SRR10968239 PRJNA603240 \n", + "10 sample_006 PRJNA603240 SRR10968240 PRJNA603240 \n", + " mgx_run_accession sample_type\n", + "1 SRR10968263 deadwood \n", + "2 SRR10968264 deadwood \n", + "3 SRR10968265 deadwood \n", + "4 SRR10968266 deadwood \n", + "5 SRR10968267 deadwood \n", + "6 SRR10968225 deadwood \n", + "7 SRR10968226 deadwood \n", + "8 SRR10968227 deadwood \n", + "9 SRR10968228 deadwood \n", + "10 SRR10968229 deadwood " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna603240_mtx <- prjna603240 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " filter(library_selection == \"RANDOM PCR\") %>%\n", + " select(sample_name = sample_alias,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna603240_mgx <- prjna603240 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna603240_mtx_vs_mgx <- left_join(prjna603240_mtx, prjna603240_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"deadwood\")\n", + "\n", + "prjna603240_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "4bf62859", + "metadata": {}, + "source": [ + "## PRJNA202380" + ] + }, + { + "cell_type": "code", + "execution_count": 30, + "id": "10459508", + "metadata": {}, + "outputs": [], + "source": [ + "prjna202380 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA202380&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 31, + "id": "de539bd6", + "metadata": {}, + "outputs": [], + "source": [ + "prjna202380 <- prjna202380 %>%\n", + " filter(instrument_platform == \"ILLUMINA\") %>%\n", + " mutate(sample_name = gsub(\"Illumina HiSeq 2000 paired end sequencing; \", \"\", experiment_title),\n", + " sample_name = gsub(\" metatranscri[op]tomic sample\", \"\", sample_name),\n", + " sample_name = gsub(\" metagenomic sample\", \"\", sample_name),\n", + " sample_name = gsub(\"Illumina HiSeq 2000 sequencing; \", \"\", sample_name)) %>%\n", + " select(study_accession, run_accession,library_source, sample_name)" + ] + }, + { + "cell_type": "code", + "execution_count": 32, + "id": "4b7fc613", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 20 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
Tag1363-1PRJNA202380SRR1138694PRJNA202380SRR1267595sheep_rumen
Tag1111-1PRJNA202380SRR1138697PRJNA202380SRR1222429sheep_rumen
Tag1111-2PRJNA202380SRR1138702PRJNA202380SRR1222431sheep_rumen
Tag1234-1PRJNA202380SRR1206249PRJNA202380SRR1206671sheep_rumen
Tag1494-1PRJNA202380SRR873450 PRJNA202380SRR873595 sheep_rumen
Tag1283-1PRJNA202380SRR873451 PRJNA202380SRR873596 sheep_rumen
Tag1435-1PRJNA202380SRR873452 PRJNA202380SRR873597 sheep_rumen
Tag1494-2PRJNA202380SRR873453 PRJNA202380SRR873598 sheep_rumen
Tag1265-1PRJNA202380SRR873454 PRJNA202380SRR873599 sheep_rumen
Tag1435-2PRJNA202380SRR873455 PRJNA202380SRR873600 sheep_rumen
Tag1283-2PRJNA202380SRR873456 PRJNA202380SRR873601 sheep_rumen
Tag1265-2PRJNA202380SRR873457 PRJNA202380SRR873602 sheep_rumen
Tag1363-2PRJNA202380SRR873458 PRJNA202380SRR873603 sheep_rumen
Tag1174-1PRJNA202380SRR873459 PRJNA202380SRR873604 sheep_rumen
Tag1234-2PRJNA202380SRR873460 PRJNA202380SRR873605 sheep_rumen
Tag1586-1PRJNA202380SRR873461 PRJNA202380SRR873606 sheep_rumen
Tag1586-2PRJNA202380SRR873462 PRJNA202380SRR873607 sheep_rumen
Tag1333-1PRJNA202380SRR873463 PRJNA202380SRR873608 sheep_rumen
Tag1174-2PRJNA202380SRR873464 PRJNA202380SRR873609 sheep_rumen
Tag1333-2PRJNA202380SRR873465 PRJNA202380SRR873610 sheep_rumen
\n" + ], + "text/latex": [ + "A tibble: 20 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t Tag1363-1 & PRJNA202380 & SRR1138694 & PRJNA202380 & SRR1267595 & sheep\\_rumen\\\\\n", + "\t Tag1111-1 & PRJNA202380 & SRR1138697 & PRJNA202380 & SRR1222429 & sheep\\_rumen\\\\\n", + "\t Tag1111-2 & PRJNA202380 & SRR1138702 & PRJNA202380 & SRR1222431 & sheep\\_rumen\\\\\n", + "\t Tag1234-1 & PRJNA202380 & SRR1206249 & PRJNA202380 & SRR1206671 & sheep\\_rumen\\\\\n", + "\t Tag1494-1 & PRJNA202380 & SRR873450 & PRJNA202380 & SRR873595 & sheep\\_rumen\\\\\n", + "\t Tag1283-1 & PRJNA202380 & SRR873451 & PRJNA202380 & SRR873596 & sheep\\_rumen\\\\\n", + "\t Tag1435-1 & PRJNA202380 & SRR873452 & PRJNA202380 & SRR873597 & sheep\\_rumen\\\\\n", + "\t Tag1494-2 & PRJNA202380 & SRR873453 & PRJNA202380 & SRR873598 & sheep\\_rumen\\\\\n", + "\t Tag1265-1 & PRJNA202380 & SRR873454 & PRJNA202380 & SRR873599 & sheep\\_rumen\\\\\n", + "\t Tag1435-2 & PRJNA202380 & SRR873455 & PRJNA202380 & SRR873600 & sheep\\_rumen\\\\\n", + "\t Tag1283-2 & PRJNA202380 & SRR873456 & PRJNA202380 & SRR873601 & sheep\\_rumen\\\\\n", + "\t Tag1265-2 & PRJNA202380 & SRR873457 & PRJNA202380 & SRR873602 & sheep\\_rumen\\\\\n", + "\t Tag1363-2 & PRJNA202380 & SRR873458 & PRJNA202380 & SRR873603 & sheep\\_rumen\\\\\n", + "\t Tag1174-1 & PRJNA202380 & SRR873459 & PRJNA202380 & SRR873604 & sheep\\_rumen\\\\\n", + "\t Tag1234-2 & PRJNA202380 & SRR873460 & PRJNA202380 & SRR873605 & sheep\\_rumen\\\\\n", + "\t Tag1586-1 & PRJNA202380 & SRR873461 & PRJNA202380 & SRR873606 & sheep\\_rumen\\\\\n", + "\t Tag1586-2 & PRJNA202380 & SRR873462 & PRJNA202380 & SRR873607 & sheep\\_rumen\\\\\n", + "\t Tag1333-1 & PRJNA202380 & SRR873463 & PRJNA202380 & SRR873608 & sheep\\_rumen\\\\\n", + "\t Tag1174-2 & PRJNA202380 & SRR873464 & PRJNA202380 & SRR873609 & sheep\\_rumen\\\\\n", + "\t Tag1333-2 & PRJNA202380 & SRR873465 & PRJNA202380 & SRR873610 & sheep\\_rumen\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 20 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| Tag1363-1 | PRJNA202380 | SRR1138694 | PRJNA202380 | SRR1267595 | sheep_rumen |\n", + "| Tag1111-1 | PRJNA202380 | SRR1138697 | PRJNA202380 | SRR1222429 | sheep_rumen |\n", + "| Tag1111-2 | PRJNA202380 | SRR1138702 | PRJNA202380 | SRR1222431 | sheep_rumen |\n", + "| Tag1234-1 | PRJNA202380 | SRR1206249 | PRJNA202380 | SRR1206671 | sheep_rumen |\n", + "| Tag1494-1 | PRJNA202380 | SRR873450 | PRJNA202380 | SRR873595 | sheep_rumen |\n", + "| Tag1283-1 | PRJNA202380 | SRR873451 | PRJNA202380 | SRR873596 | sheep_rumen |\n", + "| Tag1435-1 | PRJNA202380 | SRR873452 | PRJNA202380 | SRR873597 | sheep_rumen |\n", + "| Tag1494-2 | PRJNA202380 | SRR873453 | PRJNA202380 | SRR873598 | sheep_rumen |\n", + "| Tag1265-1 | PRJNA202380 | SRR873454 | PRJNA202380 | SRR873599 | sheep_rumen |\n", + "| Tag1435-2 | PRJNA202380 | SRR873455 | PRJNA202380 | SRR873600 | sheep_rumen |\n", + "| Tag1283-2 | PRJNA202380 | SRR873456 | PRJNA202380 | SRR873601 | sheep_rumen |\n", + "| Tag1265-2 | PRJNA202380 | SRR873457 | PRJNA202380 | SRR873602 | sheep_rumen |\n", + "| Tag1363-2 | PRJNA202380 | SRR873458 | PRJNA202380 | SRR873603 | sheep_rumen |\n", + "| Tag1174-1 | PRJNA202380 | SRR873459 | PRJNA202380 | SRR873604 | sheep_rumen |\n", + "| Tag1234-2 | PRJNA202380 | SRR873460 | PRJNA202380 | SRR873605 | sheep_rumen |\n", + "| Tag1586-1 | PRJNA202380 | SRR873461 | PRJNA202380 | SRR873606 | sheep_rumen |\n", + "| Tag1586-2 | PRJNA202380 | SRR873462 | PRJNA202380 | SRR873607 | sheep_rumen |\n", + "| Tag1333-1 | PRJNA202380 | SRR873463 | PRJNA202380 | SRR873608 | sheep_rumen |\n", + "| Tag1174-2 | PRJNA202380 | SRR873464 | PRJNA202380 | SRR873609 | sheep_rumen |\n", + "| Tag1333-2 | PRJNA202380 | SRR873465 | PRJNA202380 | SRR873610 | sheep_rumen |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 Tag1363-1 PRJNA202380 SRR1138694 PRJNA202380 \n", + "2 Tag1111-1 PRJNA202380 SRR1138697 PRJNA202380 \n", + "3 Tag1111-2 PRJNA202380 SRR1138702 PRJNA202380 \n", + "4 Tag1234-1 PRJNA202380 SRR1206249 PRJNA202380 \n", + "5 Tag1494-1 PRJNA202380 SRR873450 PRJNA202380 \n", + "6 Tag1283-1 PRJNA202380 SRR873451 PRJNA202380 \n", + "7 Tag1435-1 PRJNA202380 SRR873452 PRJNA202380 \n", + "8 Tag1494-2 PRJNA202380 SRR873453 PRJNA202380 \n", + "9 Tag1265-1 PRJNA202380 SRR873454 PRJNA202380 \n", + "10 Tag1435-2 PRJNA202380 SRR873455 PRJNA202380 \n", + "11 Tag1283-2 PRJNA202380 SRR873456 PRJNA202380 \n", + "12 Tag1265-2 PRJNA202380 SRR873457 PRJNA202380 \n", + "13 Tag1363-2 PRJNA202380 SRR873458 PRJNA202380 \n", + "14 Tag1174-1 PRJNA202380 SRR873459 PRJNA202380 \n", + "15 Tag1234-2 PRJNA202380 SRR873460 PRJNA202380 \n", + "16 Tag1586-1 PRJNA202380 SRR873461 PRJNA202380 \n", + "17 Tag1586-2 PRJNA202380 SRR873462 PRJNA202380 \n", + "18 Tag1333-1 PRJNA202380 SRR873463 PRJNA202380 \n", + "19 Tag1174-2 PRJNA202380 SRR873464 PRJNA202380 \n", + "20 Tag1333-2 PRJNA202380 SRR873465 PRJNA202380 \n", + " mgx_run_accession sample_type\n", + "1 SRR1267595 sheep_rumen\n", + "2 SRR1222429 sheep_rumen\n", + "3 SRR1222431 sheep_rumen\n", + "4 SRR1206671 sheep_rumen\n", + "5 SRR873595 sheep_rumen\n", + "6 SRR873596 sheep_rumen\n", + "7 SRR873597 sheep_rumen\n", + "8 SRR873598 sheep_rumen\n", + "9 SRR873599 sheep_rumen\n", + "10 SRR873600 sheep_rumen\n", + "11 SRR873601 sheep_rumen\n", + "12 SRR873602 sheep_rumen\n", + "13 SRR873603 sheep_rumen\n", + "14 SRR873604 sheep_rumen\n", + "15 SRR873605 sheep_rumen\n", + "16 SRR873606 sheep_rumen\n", + "17 SRR873607 sheep_rumen\n", + "18 SRR873608 sheep_rumen\n", + "19 SRR873609 sheep_rumen\n", + "20 SRR873610 sheep_rumen" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna202380_mtx <- prjna202380 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna202380_mgx <- prjna202380 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " select(sample_name,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna202380_mtx_vs_mgx <- left_join(prjna202380_mtx, prjna202380_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"sheep_rumen\")\n", + "\n", + "prjna202380_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "cde73933", + "metadata": {}, + "source": [ + "## PRJNA541981" + ] + }, + { + "cell_type": "code", + "execution_count": 33, + "id": "1a764bbb", + "metadata": {}, + "outputs": [], + "source": [ + "prjna541981 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA541981&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 34, + "id": "378b1ac3", + "metadata": {}, + "outputs": [], + "source": [ + "prjna541981_mtx <- prjna541981 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna541981_mgx <- prjna541981 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " filter(library_strategy == \"WGS\") %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna541981_mtx_vs_mgx <- left_join(prjna541981_mtx, prjna541981_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"human_skin\")" + ] + }, + { + "cell_type": "markdown", + "id": "ae3f271d", + "metadata": {}, + "source": [ + "## PRJNA797778" + ] + }, + { + "cell_type": "code", + "execution_count": 35, + "id": "a1b07276", + "metadata": {}, + "outputs": [], + "source": [ + "prjna797778 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA797778&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 36, + "id": "d479bfe9", + "metadata": { + "scrolled": true + }, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 180 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
UAB088_W2D1 PRJNA797778SRR17858151PRJNA797778SRR17635672human_vagina
UAB082_W10D2PRJNA797778SRR17858152PRJNA797778SRR17635674human_vagina
UAB082_W5D7 PRJNA797778SRR17858153PRJNA797778SRR17635676human_vagina
UAB082_W3D7 PRJNA797778SRR17858154PRJNA797778SRR17635677human_vagina
UAB082_W2D5 PRJNA797778SRR17858155PRJNA797778SRR17635678human_vagina
UAB079_W10D1PRJNA797778SRR17858156PRJNA797778SRR17635679human_vagina
UAB079_W7D6 PRJNA797778SRR17858157PRJNA797778SRR17635680human_vagina
UAB079_W6D7 PRJNA797778SRR17858158PRJNA797778SRR17635681human_vagina
UAB079_W3D7 PRJNA797778SRR17858159PRJNA797778SRR17635682human_vagina
UAB079_W2D2 PRJNA797778SRR17858160PRJNA797778SRR17635683human_vagina
UAB006_W2D2 PRJNA797778SRR17858161PRJNA797778SRR17635801human_vagina
UAB077_W9D7 PRJNA797778SRR17858162PRJNA797778SRR17635685human_vagina
UAB077_W8D2 PRJNA797778SRR17858163PRJNA797778SRR17635686human_vagina
UAB077_W5D7 PRJNA797778SRR17858164PRJNA797778SRR17635687human_vagina
UAB077_W3D1 PRJNA797778SRR17858165PRJNA797778SRR17635688human_vagina
UAB077_W1D7 PRJNA797778SRR17858166PRJNA797778SRR17635689human_vagina
UAB071_W10D2PRJNA797778SRR17858167PRJNA797778SRR17635690human_vagina
UAB071_W7D7 PRJNA797778SRR17858168PRJNA797778SRR17635691human_vagina
UAB071_W5D7 PRJNA797778SRR17858169PRJNA797778SRR17635692human_vagina
UAB071_W3D7 PRJNA797778SRR17858170PRJNA797778SRR17635693human_vagina
UAB071_W1D7 PRJNA797778SRR17858171PRJNA797778SRR17635694human_vagina
UAB002_W5D7 PRJNA797778SRR17858172PRJNA797778SRR17635804human_vagina
AYAC02_W9D3 PRJNA797778SRR17858173PRJNA797778SRR17635818human_vagina
UAB060_W10D2PRJNA797778SRR17858174PRJNA797778SRR17635696human_vagina
UAB060_W7D7 PRJNA797778SRR17858175PRJNA797778SRR17635697human_vagina
UAB060_W5D7 PRJNA797778SRR17858176PRJNA797778SRR17635698human_vagina
UAB060_W3D7 PRJNA797778SRR17858177PRJNA797778SRR17635763human_vagina
UAB060_W1D6 PRJNA797778SRR17858178PRJNA797778SRR17635764human_vagina
UAB052_W10D2PRJNA797778SRR17858179PRJNA797778SRR17635765human_vagina
UAB052_W8D3 PRJNA797778SRR17858180PRJNA797778SRR17635766human_vagina
UAB116_W1D7 PRJNA797778SRR17858301PRJNA797778SRR17635741human_vagina
UAB110_W10D4PRJNA797778SRR17858302PRJNA797778SRR17635742human_vagina
UAB110_W7D7 PRJNA797778SRR17858303PRJNA797778SRR17635743human_vagina
UAB110_W6D2 PRJNA797778SRR17858304PRJNA797778SRR17635744human_vagina
UAB110_W3D7 PRJNA797778SRR17858305PRJNA797778SRR17635745human_vagina
UAB010_W5D6 PRJNA797778SRR17858306PRJNA797778SRR17635490human_vagina
UAB110_W1D7 PRJNA797778SRR17858307PRJNA797778SRR17635746human_vagina
UAB106_W9D7 PRJNA797778SRR17858308PRJNA797778SRR17635748human_vagina
UAB106_W7D7 PRJNA797778SRR17858309PRJNA797778SRR17635749human_vagina
UAB106_W5D4 PRJNA797778SRR17858310PRJNA797778SRR17635750human_vagina
UAB106_W3D7 PRJNA797778SRR17858311PRJNA797778SRR17635751human_vagina
UAB106_W1D7 PRJNA797778SRR17858312PRJNA797778SRR17635752human_vagina
UAB096_W9D7 PRJNA797778SRR17858313PRJNA797778SRR17635753human_vagina
UAB096_W7D3 PRJNA797778SRR17858314PRJNA797778SRR17635754human_vagina
UAB096_W5D3 PRJNA797778SRR17858315PRJNA797778SRR17635755human_vagina
UAB096_W3D3 PRJNA797778SRR17858316PRJNA797778SRR17635756human_vagina
UAB010_W3D1 PRJNA797778SRR17858317PRJNA797778SRR17635491human_vagina
UAB096_W1D5 PRJNA797778SRR17858318PRJNA797778SRR17635757human_vagina
UAB093_W9D6 PRJNA797778SRR17858319PRJNA797778SRR17635759human_vagina
UAB093_W7D7 PRJNA797778SRR17858320PRJNA797778SRR17635760human_vagina
UAB093_W5D7 PRJNA797778SRR17858321PRJNA797778SRR17635761human_vagina
UAB093_W4D2 PRJNA797778SRR17858322PRJNA797778SRR17635762human_vagina
UAB093_W1D7 PRJNA797778SRR17858323PRJNA797778SRR17635667human_vagina
UAB088_W10D1PRJNA797778SRR17858324PRJNA797778SRR17635668human_vagina
UAB088_W7D6 PRJNA797778SRR17858325PRJNA797778SRR17635669human_vagina
UAB088_W6D1 PRJNA797778SRR17858326PRJNA797778SRR17635670human_vagina
UAB088_W4D1 PRJNA797778SRR17858327PRJNA797778SRR17635671human_vagina
UAB007_W1D7 PRJNA797778SRR17858328PRJNA797778SRR17635795human_vagina
AYAC02_W1D7 PRJNA797778SRR17858329PRJNA797778SRR17635794human_vagina
EM04_W3D7 PRJNA797778SRR17858330PRJNA797778SRR17635711human_vagina
\n" + ], + "text/latex": [ + "A tibble: 180 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t UAB088\\_W2D1 & PRJNA797778 & SRR17858151 & PRJNA797778 & SRR17635672 & human\\_vagina\\\\\n", + "\t UAB082\\_W10D2 & PRJNA797778 & SRR17858152 & PRJNA797778 & SRR17635674 & human\\_vagina\\\\\n", + "\t UAB082\\_W5D7 & PRJNA797778 & SRR17858153 & PRJNA797778 & SRR17635676 & human\\_vagina\\\\\n", + "\t UAB082\\_W3D7 & PRJNA797778 & SRR17858154 & PRJNA797778 & SRR17635677 & human\\_vagina\\\\\n", + "\t UAB082\\_W2D5 & PRJNA797778 & SRR17858155 & PRJNA797778 & SRR17635678 & human\\_vagina\\\\\n", + "\t UAB079\\_W10D1 & PRJNA797778 & SRR17858156 & PRJNA797778 & SRR17635679 & human\\_vagina\\\\\n", + "\t UAB079\\_W7D6 & PRJNA797778 & SRR17858157 & PRJNA797778 & SRR17635680 & human\\_vagina\\\\\n", + "\t UAB079\\_W6D7 & PRJNA797778 & SRR17858158 & PRJNA797778 & SRR17635681 & human\\_vagina\\\\\n", + "\t UAB079\\_W3D7 & PRJNA797778 & SRR17858159 & PRJNA797778 & SRR17635682 & human\\_vagina\\\\\n", + "\t UAB079\\_W2D2 & PRJNA797778 & SRR17858160 & PRJNA797778 & SRR17635683 & human\\_vagina\\\\\n", + "\t UAB006\\_W2D2 & PRJNA797778 & SRR17858161 & PRJNA797778 & SRR17635801 & human\\_vagina\\\\\n", + "\t UAB077\\_W9D7 & PRJNA797778 & SRR17858162 & PRJNA797778 & SRR17635685 & human\\_vagina\\\\\n", + "\t UAB077\\_W8D2 & PRJNA797778 & SRR17858163 & PRJNA797778 & SRR17635686 & human\\_vagina\\\\\n", + "\t UAB077\\_W5D7 & PRJNA797778 & SRR17858164 & PRJNA797778 & SRR17635687 & human\\_vagina\\\\\n", + "\t UAB077\\_W3D1 & PRJNA797778 & SRR17858165 & PRJNA797778 & SRR17635688 & human\\_vagina\\\\\n", + "\t UAB077\\_W1D7 & PRJNA797778 & SRR17858166 & PRJNA797778 & SRR17635689 & human\\_vagina\\\\\n", + "\t UAB071\\_W10D2 & PRJNA797778 & SRR17858167 & PRJNA797778 & SRR17635690 & human\\_vagina\\\\\n", + "\t UAB071\\_W7D7 & PRJNA797778 & SRR17858168 & PRJNA797778 & SRR17635691 & human\\_vagina\\\\\n", + "\t UAB071\\_W5D7 & PRJNA797778 & SRR17858169 & PRJNA797778 & SRR17635692 & human\\_vagina\\\\\n", + "\t UAB071\\_W3D7 & PRJNA797778 & SRR17858170 & PRJNA797778 & SRR17635693 & human\\_vagina\\\\\n", + "\t UAB071\\_W1D7 & PRJNA797778 & SRR17858171 & PRJNA797778 & SRR17635694 & human\\_vagina\\\\\n", + "\t UAB002\\_W5D7 & PRJNA797778 & SRR17858172 & PRJNA797778 & SRR17635804 & human\\_vagina\\\\\n", + "\t AYAC02\\_W9D3 & PRJNA797778 & SRR17858173 & PRJNA797778 & SRR17635818 & human\\_vagina\\\\\n", + "\t UAB060\\_W10D2 & PRJNA797778 & SRR17858174 & PRJNA797778 & SRR17635696 & human\\_vagina\\\\\n", + "\t UAB060\\_W7D7 & PRJNA797778 & SRR17858175 & PRJNA797778 & SRR17635697 & human\\_vagina\\\\\n", + "\t UAB060\\_W5D7 & PRJNA797778 & SRR17858176 & PRJNA797778 & SRR17635698 & human\\_vagina\\\\\n", + "\t UAB060\\_W3D7 & PRJNA797778 & SRR17858177 & PRJNA797778 & SRR17635763 & human\\_vagina\\\\\n", + "\t UAB060\\_W1D6 & PRJNA797778 & SRR17858178 & PRJNA797778 & SRR17635764 & human\\_vagina\\\\\n", + "\t UAB052\\_W10D2 & PRJNA797778 & SRR17858179 & PRJNA797778 & SRR17635765 & human\\_vagina\\\\\n", + "\t UAB052\\_W8D3 & PRJNA797778 & SRR17858180 & PRJNA797778 & SRR17635766 & human\\_vagina\\\\\n", + "\t ⋮ & ⋮ & ⋮ & ⋮ & ⋮ & ⋮\\\\\n", + "\t UAB116\\_W1D7 & PRJNA797778 & SRR17858301 & PRJNA797778 & SRR17635741 & human\\_vagina\\\\\n", + "\t UAB110\\_W10D4 & PRJNA797778 & SRR17858302 & PRJNA797778 & SRR17635742 & human\\_vagina\\\\\n", + "\t UAB110\\_W7D7 & PRJNA797778 & SRR17858303 & PRJNA797778 & SRR17635743 & human\\_vagina\\\\\n", + "\t UAB110\\_W6D2 & PRJNA797778 & SRR17858304 & PRJNA797778 & SRR17635744 & human\\_vagina\\\\\n", + "\t UAB110\\_W3D7 & PRJNA797778 & SRR17858305 & PRJNA797778 & SRR17635745 & human\\_vagina\\\\\n", + "\t UAB010\\_W5D6 & PRJNA797778 & SRR17858306 & PRJNA797778 & SRR17635490 & human\\_vagina\\\\\n", + "\t UAB110\\_W1D7 & PRJNA797778 & SRR17858307 & PRJNA797778 & SRR17635746 & human\\_vagina\\\\\n", + "\t UAB106\\_W9D7 & PRJNA797778 & SRR17858308 & PRJNA797778 & SRR17635748 & human\\_vagina\\\\\n", + "\t UAB106\\_W7D7 & PRJNA797778 & SRR17858309 & PRJNA797778 & SRR17635749 & human\\_vagina\\\\\n", + "\t UAB106\\_W5D4 & PRJNA797778 & SRR17858310 & PRJNA797778 & SRR17635750 & human\\_vagina\\\\\n", + "\t UAB106\\_W3D7 & PRJNA797778 & SRR17858311 & PRJNA797778 & SRR17635751 & human\\_vagina\\\\\n", + "\t UAB106\\_W1D7 & PRJNA797778 & SRR17858312 & PRJNA797778 & SRR17635752 & human\\_vagina\\\\\n", + "\t UAB096\\_W9D7 & PRJNA797778 & SRR17858313 & PRJNA797778 & SRR17635753 & human\\_vagina\\\\\n", + "\t UAB096\\_W7D3 & PRJNA797778 & SRR17858314 & PRJNA797778 & SRR17635754 & human\\_vagina\\\\\n", + "\t UAB096\\_W5D3 & PRJNA797778 & SRR17858315 & PRJNA797778 & SRR17635755 & human\\_vagina\\\\\n", + "\t UAB096\\_W3D3 & PRJNA797778 & SRR17858316 & PRJNA797778 & SRR17635756 & human\\_vagina\\\\\n", + "\t UAB010\\_W3D1 & PRJNA797778 & SRR17858317 & PRJNA797778 & SRR17635491 & human\\_vagina\\\\\n", + "\t UAB096\\_W1D5 & PRJNA797778 & SRR17858318 & PRJNA797778 & SRR17635757 & human\\_vagina\\\\\n", + "\t UAB093\\_W9D6 & PRJNA797778 & SRR17858319 & PRJNA797778 & SRR17635759 & human\\_vagina\\\\\n", + "\t UAB093\\_W7D7 & PRJNA797778 & SRR17858320 & PRJNA797778 & SRR17635760 & human\\_vagina\\\\\n", + "\t UAB093\\_W5D7 & PRJNA797778 & SRR17858321 & PRJNA797778 & SRR17635761 & human\\_vagina\\\\\n", + "\t UAB093\\_W4D2 & PRJNA797778 & SRR17858322 & PRJNA797778 & SRR17635762 & human\\_vagina\\\\\n", + "\t UAB093\\_W1D7 & PRJNA797778 & SRR17858323 & PRJNA797778 & SRR17635667 & human\\_vagina\\\\\n", + "\t UAB088\\_W10D1 & PRJNA797778 & SRR17858324 & PRJNA797778 & SRR17635668 & human\\_vagina\\\\\n", + "\t UAB088\\_W7D6 & PRJNA797778 & SRR17858325 & PRJNA797778 & SRR17635669 & human\\_vagina\\\\\n", + "\t UAB088\\_W6D1 & PRJNA797778 & SRR17858326 & PRJNA797778 & SRR17635670 & human\\_vagina\\\\\n", + "\t UAB088\\_W4D1 & PRJNA797778 & SRR17858327 & PRJNA797778 & SRR17635671 & human\\_vagina\\\\\n", + "\t UAB007\\_W1D7 & PRJNA797778 & SRR17858328 & PRJNA797778 & SRR17635795 & human\\_vagina\\\\\n", + "\t AYAC02\\_W1D7 & PRJNA797778 & SRR17858329 & PRJNA797778 & SRR17635794 & human\\_vagina\\\\\n", + "\t EM04\\_W3D7 & PRJNA797778 & SRR17858330 & PRJNA797778 & SRR17635711 & human\\_vagina\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 180 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| UAB088_W2D1 | PRJNA797778 | SRR17858151 | PRJNA797778 | SRR17635672 | human_vagina |\n", + "| UAB082_W10D2 | PRJNA797778 | SRR17858152 | PRJNA797778 | SRR17635674 | human_vagina |\n", + "| UAB082_W5D7 | PRJNA797778 | SRR17858153 | PRJNA797778 | SRR17635676 | human_vagina |\n", + "| UAB082_W3D7 | PRJNA797778 | SRR17858154 | PRJNA797778 | SRR17635677 | human_vagina |\n", + "| UAB082_W2D5 | PRJNA797778 | SRR17858155 | PRJNA797778 | SRR17635678 | human_vagina |\n", + "| UAB079_W10D1 | PRJNA797778 | SRR17858156 | PRJNA797778 | SRR17635679 | human_vagina |\n", + "| UAB079_W7D6 | PRJNA797778 | SRR17858157 | PRJNA797778 | SRR17635680 | human_vagina |\n", + "| UAB079_W6D7 | PRJNA797778 | SRR17858158 | PRJNA797778 | SRR17635681 | human_vagina |\n", + "| UAB079_W3D7 | PRJNA797778 | SRR17858159 | PRJNA797778 | SRR17635682 | human_vagina |\n", + "| UAB079_W2D2 | PRJNA797778 | SRR17858160 | PRJNA797778 | SRR17635683 | human_vagina |\n", + "| UAB006_W2D2 | PRJNA797778 | SRR17858161 | PRJNA797778 | SRR17635801 | human_vagina |\n", + "| UAB077_W9D7 | PRJNA797778 | SRR17858162 | PRJNA797778 | SRR17635685 | human_vagina |\n", + "| UAB077_W8D2 | PRJNA797778 | SRR17858163 | PRJNA797778 | SRR17635686 | human_vagina |\n", + "| UAB077_W5D7 | PRJNA797778 | SRR17858164 | PRJNA797778 | SRR17635687 | human_vagina |\n", + "| UAB077_W3D1 | PRJNA797778 | SRR17858165 | PRJNA797778 | SRR17635688 | human_vagina |\n", + "| UAB077_W1D7 | PRJNA797778 | SRR17858166 | PRJNA797778 | SRR17635689 | human_vagina |\n", + "| UAB071_W10D2 | PRJNA797778 | SRR17858167 | PRJNA797778 | SRR17635690 | human_vagina |\n", + "| UAB071_W7D7 | PRJNA797778 | SRR17858168 | PRJNA797778 | SRR17635691 | human_vagina |\n", + "| UAB071_W5D7 | PRJNA797778 | SRR17858169 | PRJNA797778 | SRR17635692 | human_vagina |\n", + "| UAB071_W3D7 | PRJNA797778 | SRR17858170 | PRJNA797778 | SRR17635693 | human_vagina |\n", + "| UAB071_W1D7 | PRJNA797778 | SRR17858171 | PRJNA797778 | SRR17635694 | human_vagina |\n", + "| UAB002_W5D7 | PRJNA797778 | SRR17858172 | PRJNA797778 | SRR17635804 | human_vagina |\n", + "| AYAC02_W9D3 | PRJNA797778 | SRR17858173 | PRJNA797778 | SRR17635818 | human_vagina |\n", + "| UAB060_W10D2 | PRJNA797778 | SRR17858174 | PRJNA797778 | SRR17635696 | human_vagina |\n", + "| UAB060_W7D7 | PRJNA797778 | SRR17858175 | PRJNA797778 | SRR17635697 | human_vagina |\n", + "| UAB060_W5D7 | PRJNA797778 | SRR17858176 | PRJNA797778 | SRR17635698 | human_vagina |\n", + "| UAB060_W3D7 | PRJNA797778 | SRR17858177 | PRJNA797778 | SRR17635763 | human_vagina |\n", + "| UAB060_W1D6 | PRJNA797778 | SRR17858178 | PRJNA797778 | SRR17635764 | human_vagina |\n", + "| UAB052_W10D2 | PRJNA797778 | SRR17858179 | PRJNA797778 | SRR17635765 | human_vagina |\n", + "| UAB052_W8D3 | PRJNA797778 | SRR17858180 | PRJNA797778 | SRR17635766 | human_vagina |\n", + "| ⋮ | ⋮ | ⋮ | ⋮ | ⋮ | ⋮ |\n", + "| UAB116_W1D7 | PRJNA797778 | SRR17858301 | PRJNA797778 | SRR17635741 | human_vagina |\n", + "| UAB110_W10D4 | PRJNA797778 | SRR17858302 | PRJNA797778 | SRR17635742 | human_vagina |\n", + "| UAB110_W7D7 | PRJNA797778 | SRR17858303 | PRJNA797778 | SRR17635743 | human_vagina |\n", + "| UAB110_W6D2 | PRJNA797778 | SRR17858304 | PRJNA797778 | SRR17635744 | human_vagina |\n", + "| UAB110_W3D7 | PRJNA797778 | SRR17858305 | PRJNA797778 | SRR17635745 | human_vagina |\n", + "| UAB010_W5D6 | PRJNA797778 | SRR17858306 | PRJNA797778 | SRR17635490 | human_vagina |\n", + "| UAB110_W1D7 | PRJNA797778 | SRR17858307 | PRJNA797778 | SRR17635746 | human_vagina |\n", + "| UAB106_W9D7 | PRJNA797778 | SRR17858308 | PRJNA797778 | SRR17635748 | human_vagina |\n", + "| UAB106_W7D7 | PRJNA797778 | SRR17858309 | PRJNA797778 | SRR17635749 | human_vagina |\n", + "| UAB106_W5D4 | PRJNA797778 | SRR17858310 | PRJNA797778 | SRR17635750 | human_vagina |\n", + "| UAB106_W3D7 | PRJNA797778 | SRR17858311 | PRJNA797778 | SRR17635751 | human_vagina |\n", + "| UAB106_W1D7 | PRJNA797778 | SRR17858312 | PRJNA797778 | SRR17635752 | human_vagina |\n", + "| UAB096_W9D7 | PRJNA797778 | SRR17858313 | PRJNA797778 | SRR17635753 | human_vagina |\n", + "| UAB096_W7D3 | PRJNA797778 | SRR17858314 | PRJNA797778 | SRR17635754 | human_vagina |\n", + "| UAB096_W5D3 | PRJNA797778 | SRR17858315 | PRJNA797778 | SRR17635755 | human_vagina |\n", + "| UAB096_W3D3 | PRJNA797778 | SRR17858316 | PRJNA797778 | SRR17635756 | human_vagina |\n", + "| UAB010_W3D1 | PRJNA797778 | SRR17858317 | PRJNA797778 | SRR17635491 | human_vagina |\n", + "| UAB096_W1D5 | PRJNA797778 | SRR17858318 | PRJNA797778 | SRR17635757 | human_vagina |\n", + "| UAB093_W9D6 | PRJNA797778 | SRR17858319 | PRJNA797778 | SRR17635759 | human_vagina |\n", + "| UAB093_W7D7 | PRJNA797778 | SRR17858320 | PRJNA797778 | SRR17635760 | human_vagina |\n", + "| UAB093_W5D7 | PRJNA797778 | SRR17858321 | PRJNA797778 | SRR17635761 | human_vagina |\n", + "| UAB093_W4D2 | PRJNA797778 | SRR17858322 | PRJNA797778 | SRR17635762 | human_vagina |\n", + "| UAB093_W1D7 | PRJNA797778 | SRR17858323 | PRJNA797778 | SRR17635667 | human_vagina |\n", + "| UAB088_W10D1 | PRJNA797778 | SRR17858324 | PRJNA797778 | SRR17635668 | human_vagina |\n", + "| UAB088_W7D6 | PRJNA797778 | SRR17858325 | PRJNA797778 | SRR17635669 | human_vagina |\n", + "| UAB088_W6D1 | PRJNA797778 | SRR17858326 | PRJNA797778 | SRR17635670 | human_vagina |\n", + "| UAB088_W4D1 | PRJNA797778 | SRR17858327 | PRJNA797778 | SRR17635671 | human_vagina |\n", + "| UAB007_W1D7 | PRJNA797778 | SRR17858328 | PRJNA797778 | SRR17635795 | human_vagina |\n", + "| AYAC02_W1D7 | PRJNA797778 | SRR17858329 | PRJNA797778 | SRR17635794 | human_vagina |\n", + "| EM04_W3D7 | PRJNA797778 | SRR17858330 | PRJNA797778 | SRR17635711 | human_vagina |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 UAB088_W2D1 PRJNA797778 SRR17858151 PRJNA797778 \n", + "2 UAB082_W10D2 PRJNA797778 SRR17858152 PRJNA797778 \n", + "3 UAB082_W5D7 PRJNA797778 SRR17858153 PRJNA797778 \n", + "4 UAB082_W3D7 PRJNA797778 SRR17858154 PRJNA797778 \n", + "5 UAB082_W2D5 PRJNA797778 SRR17858155 PRJNA797778 \n", + "6 UAB079_W10D1 PRJNA797778 SRR17858156 PRJNA797778 \n", + "7 UAB079_W7D6 PRJNA797778 SRR17858157 PRJNA797778 \n", + "8 UAB079_W6D7 PRJNA797778 SRR17858158 PRJNA797778 \n", + "9 UAB079_W3D7 PRJNA797778 SRR17858159 PRJNA797778 \n", + "10 UAB079_W2D2 PRJNA797778 SRR17858160 PRJNA797778 \n", + "11 UAB006_W2D2 PRJNA797778 SRR17858161 PRJNA797778 \n", + "12 UAB077_W9D7 PRJNA797778 SRR17858162 PRJNA797778 \n", + "13 UAB077_W8D2 PRJNA797778 SRR17858163 PRJNA797778 \n", + "14 UAB077_W5D7 PRJNA797778 SRR17858164 PRJNA797778 \n", + "15 UAB077_W3D1 PRJNA797778 SRR17858165 PRJNA797778 \n", + "16 UAB077_W1D7 PRJNA797778 SRR17858166 PRJNA797778 \n", + "17 UAB071_W10D2 PRJNA797778 SRR17858167 PRJNA797778 \n", + "18 UAB071_W7D7 PRJNA797778 SRR17858168 PRJNA797778 \n", + "19 UAB071_W5D7 PRJNA797778 SRR17858169 PRJNA797778 \n", + "20 UAB071_W3D7 PRJNA797778 SRR17858170 PRJNA797778 \n", + "21 UAB071_W1D7 PRJNA797778 SRR17858171 PRJNA797778 \n", + "22 UAB002_W5D7 PRJNA797778 SRR17858172 PRJNA797778 \n", + "23 AYAC02_W9D3 PRJNA797778 SRR17858173 PRJNA797778 \n", + "24 UAB060_W10D2 PRJNA797778 SRR17858174 PRJNA797778 \n", + "25 UAB060_W7D7 PRJNA797778 SRR17858175 PRJNA797778 \n", + "26 UAB060_W5D7 PRJNA797778 SRR17858176 PRJNA797778 \n", + "27 UAB060_W3D7 PRJNA797778 SRR17858177 PRJNA797778 \n", + "28 UAB060_W1D6 PRJNA797778 SRR17858178 PRJNA797778 \n", + "29 UAB052_W10D2 PRJNA797778 SRR17858179 PRJNA797778 \n", + "30 UAB052_W8D3 PRJNA797778 SRR17858180 PRJNA797778 \n", + "⋮ ⋮ ⋮ ⋮ ⋮ \n", + "151 UAB116_W1D7 PRJNA797778 SRR17858301 PRJNA797778 \n", + "152 UAB110_W10D4 PRJNA797778 SRR17858302 PRJNA797778 \n", + "153 UAB110_W7D7 PRJNA797778 SRR17858303 PRJNA797778 \n", + "154 UAB110_W6D2 PRJNA797778 SRR17858304 PRJNA797778 \n", + "155 UAB110_W3D7 PRJNA797778 SRR17858305 PRJNA797778 \n", + "156 UAB010_W5D6 PRJNA797778 SRR17858306 PRJNA797778 \n", + "157 UAB110_W1D7 PRJNA797778 SRR17858307 PRJNA797778 \n", + "158 UAB106_W9D7 PRJNA797778 SRR17858308 PRJNA797778 \n", + "159 UAB106_W7D7 PRJNA797778 SRR17858309 PRJNA797778 \n", + "160 UAB106_W5D4 PRJNA797778 SRR17858310 PRJNA797778 \n", + "161 UAB106_W3D7 PRJNA797778 SRR17858311 PRJNA797778 \n", + "162 UAB106_W1D7 PRJNA797778 SRR17858312 PRJNA797778 \n", + "163 UAB096_W9D7 PRJNA797778 SRR17858313 PRJNA797778 \n", + "164 UAB096_W7D3 PRJNA797778 SRR17858314 PRJNA797778 \n", + "165 UAB096_W5D3 PRJNA797778 SRR17858315 PRJNA797778 \n", + "166 UAB096_W3D3 PRJNA797778 SRR17858316 PRJNA797778 \n", + "167 UAB010_W3D1 PRJNA797778 SRR17858317 PRJNA797778 \n", + "168 UAB096_W1D5 PRJNA797778 SRR17858318 PRJNA797778 \n", + "169 UAB093_W9D6 PRJNA797778 SRR17858319 PRJNA797778 \n", + "170 UAB093_W7D7 PRJNA797778 SRR17858320 PRJNA797778 \n", + "171 UAB093_W5D7 PRJNA797778 SRR17858321 PRJNA797778 \n", + "172 UAB093_W4D2 PRJNA797778 SRR17858322 PRJNA797778 \n", + "173 UAB093_W1D7 PRJNA797778 SRR17858323 PRJNA797778 \n", + "174 UAB088_W10D1 PRJNA797778 SRR17858324 PRJNA797778 \n", + "175 UAB088_W7D6 PRJNA797778 SRR17858325 PRJNA797778 \n", + "176 UAB088_W6D1 PRJNA797778 SRR17858326 PRJNA797778 \n", + "177 UAB088_W4D1 PRJNA797778 SRR17858327 PRJNA797778 \n", + "178 UAB007_W1D7 PRJNA797778 SRR17858328 PRJNA797778 \n", + "179 AYAC02_W1D7 PRJNA797778 SRR17858329 PRJNA797778 \n", + "180 EM04_W3D7 PRJNA797778 SRR17858330 PRJNA797778 \n", + " mgx_run_accession sample_type \n", + "1 SRR17635672 human_vagina\n", + "2 SRR17635674 human_vagina\n", + "3 SRR17635676 human_vagina\n", + "4 SRR17635677 human_vagina\n", + "5 SRR17635678 human_vagina\n", + "6 SRR17635679 human_vagina\n", + "7 SRR17635680 human_vagina\n", + "8 SRR17635681 human_vagina\n", + "9 SRR17635682 human_vagina\n", + "10 SRR17635683 human_vagina\n", + "11 SRR17635801 human_vagina\n", + "12 SRR17635685 human_vagina\n", + "13 SRR17635686 human_vagina\n", + "14 SRR17635687 human_vagina\n", + "15 SRR17635688 human_vagina\n", + "16 SRR17635689 human_vagina\n", + "17 SRR17635690 human_vagina\n", + "18 SRR17635691 human_vagina\n", + "19 SRR17635692 human_vagina\n", + "20 SRR17635693 human_vagina\n", + "21 SRR17635694 human_vagina\n", + "22 SRR17635804 human_vagina\n", + "23 SRR17635818 human_vagina\n", + "24 SRR17635696 human_vagina\n", + "25 SRR17635697 human_vagina\n", + "26 SRR17635698 human_vagina\n", + "27 SRR17635763 human_vagina\n", + "28 SRR17635764 human_vagina\n", + "29 SRR17635765 human_vagina\n", + "30 SRR17635766 human_vagina\n", + "⋮ ⋮ ⋮ \n", + "151 SRR17635741 human_vagina\n", + "152 SRR17635742 human_vagina\n", + "153 SRR17635743 human_vagina\n", + "154 SRR17635744 human_vagina\n", + "155 SRR17635745 human_vagina\n", + "156 SRR17635490 human_vagina\n", + "157 SRR17635746 human_vagina\n", + "158 SRR17635748 human_vagina\n", + "159 SRR17635749 human_vagina\n", + "160 SRR17635750 human_vagina\n", + "161 SRR17635751 human_vagina\n", + "162 SRR17635752 human_vagina\n", + "163 SRR17635753 human_vagina\n", + "164 SRR17635754 human_vagina\n", + "165 SRR17635755 human_vagina\n", + "166 SRR17635756 human_vagina\n", + "167 SRR17635491 human_vagina\n", + "168 SRR17635757 human_vagina\n", + "169 SRR17635759 human_vagina\n", + "170 SRR17635760 human_vagina\n", + "171 SRR17635761 human_vagina\n", + "172 SRR17635762 human_vagina\n", + "173 SRR17635667 human_vagina\n", + "174 SRR17635668 human_vagina\n", + "175 SRR17635669 human_vagina\n", + "176 SRR17635670 human_vagina\n", + "177 SRR17635671 human_vagina\n", + "178 SRR17635795 human_vagina\n", + "179 SRR17635794 human_vagina\n", + "180 SRR17635711 human_vagina" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna797778_mtx <- prjna797778 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna797778_mgx <- prjna797778 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna797778_mtx_vs_mgx <- left_join(prjna797778_mtx, prjna797778_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"human_vagina\")\n", + "\n", + "prjna797778_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "e19c96ba", + "metadata": {}, + "source": [ + "## PRJNA339914" + ] + }, + { + "cell_type": "code", + "execution_count": 37, + "id": "bb6f3136", + "metadata": {}, + "outputs": [], + "source": [ + "prjna339914 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA339914&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 38, + "id": "3c6e9e34", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 4 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
MV_FEI4_t2Q15PRJNA339914SRR4100706PRJNA339914SRR4052039human_gut
MV_FEI5_t3Q15PRJNA339914SRR4100707PRJNA339914SRR4052042human_gut
MV_FEM4_t2Q15PRJNA339914SRR4100708PRJNA339914SRR4052025human_gut
MV_FEM5_t3Q15PRJNA339914SRR4100709PRJNA339914SRR4052028human_gut
\n" + ], + "text/latex": [ + "A tibble: 4 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t MV\\_FEI4\\_t2Q15 & PRJNA339914 & SRR4100706 & PRJNA339914 & SRR4052039 & human\\_gut\\\\\n", + "\t MV\\_FEI5\\_t3Q15 & PRJNA339914 & SRR4100707 & PRJNA339914 & SRR4052042 & human\\_gut\\\\\n", + "\t MV\\_FEM4\\_t2Q15 & PRJNA339914 & SRR4100708 & PRJNA339914 & SRR4052025 & human\\_gut\\\\\n", + "\t MV\\_FEM5\\_t3Q15 & PRJNA339914 & SRR4100709 & PRJNA339914 & SRR4052028 & human\\_gut\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 4 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| MV_FEI4_t2Q15 | PRJNA339914 | SRR4100706 | PRJNA339914 | SRR4052039 | human_gut |\n", + "| MV_FEI5_t3Q15 | PRJNA339914 | SRR4100707 | PRJNA339914 | SRR4052042 | human_gut |\n", + "| MV_FEM4_t2Q15 | PRJNA339914 | SRR4100708 | PRJNA339914 | SRR4052025 | human_gut |\n", + "| MV_FEM5_t3Q15 | PRJNA339914 | SRR4100709 | PRJNA339914 | SRR4052028 | human_gut |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 MV_FEI4_t2Q15 PRJNA339914 SRR4100706 PRJNA339914 \n", + "2 MV_FEI5_t3Q15 PRJNA339914 SRR4100707 PRJNA339914 \n", + "3 MV_FEM4_t2Q15 PRJNA339914 SRR4100708 PRJNA339914 \n", + "4 MV_FEM5_t3Q15 PRJNA339914 SRR4100709 PRJNA339914 \n", + " mgx_run_accession sample_type\n", + "1 SRR4052039 human_gut \n", + "2 SRR4052042 human_gut \n", + "3 SRR4052025 human_gut \n", + "4 SRR4052028 human_gut " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna339914_mtx <- prjna339914 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " mutate(sample_name = gsub(\"RNA\", \"\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna339914_mgx <- prjna339914 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna339914_mtx_vs_mgx <- left_join(prjna339914_mtx, prjna339914_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"human_gut\")\n", + "\n", + "prjna339914_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "a6999921", + "metadata": {}, + "source": [ + "## PRJEB33889" + ] + }, + { + "cell_type": "code", + "execution_count": 39, + "id": "8d446ea8", + "metadata": {}, + "outputs": [], + "source": [ + "prjeb33889 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJEB33889&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 40, + "id": "a4d19362", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 8 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
M1PRJEB33889ERR3473664PRJEB33889ERR3473656mouse_cecum
M2PRJEB33889ERR3473665PRJEB33889ERR3473657mouse_cecum
M3PRJEB33889ERR3473666PRJEB33889ERR3473658mouse_cecum
M4PRJEB33889ERR3473667PRJEB33889ERR3473659mouse_cecum
M5PRJEB33889ERR3473668PRJEB33889ERR3473660mouse_cecum
M6PRJEB33889ERR3473669PRJEB33889ERR3473661mouse_cecum
M7PRJEB33889ERR3473670PRJEB33889ERR3473662mouse_cecum
M8PRJEB33889ERR3473671PRJEB33889ERR3473663mouse_cecum
\n" + ], + "text/latex": [ + "A tibble: 8 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t M1 & PRJEB33889 & ERR3473664 & PRJEB33889 & ERR3473656 & mouse\\_cecum\\\\\n", + "\t M2 & PRJEB33889 & ERR3473665 & PRJEB33889 & ERR3473657 & mouse\\_cecum\\\\\n", + "\t M3 & PRJEB33889 & ERR3473666 & PRJEB33889 & ERR3473658 & mouse\\_cecum\\\\\n", + "\t M4 & PRJEB33889 & ERR3473667 & PRJEB33889 & ERR3473659 & mouse\\_cecum\\\\\n", + "\t M5 & PRJEB33889 & ERR3473668 & PRJEB33889 & ERR3473660 & mouse\\_cecum\\\\\n", + "\t M6 & PRJEB33889 & ERR3473669 & PRJEB33889 & ERR3473661 & mouse\\_cecum\\\\\n", + "\t M7 & PRJEB33889 & ERR3473670 & PRJEB33889 & ERR3473662 & mouse\\_cecum\\\\\n", + "\t M8 & PRJEB33889 & ERR3473671 & PRJEB33889 & ERR3473663 & mouse\\_cecum\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 8 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| M1 | PRJEB33889 | ERR3473664 | PRJEB33889 | ERR3473656 | mouse_cecum |\n", + "| M2 | PRJEB33889 | ERR3473665 | PRJEB33889 | ERR3473657 | mouse_cecum |\n", + "| M3 | PRJEB33889 | ERR3473666 | PRJEB33889 | ERR3473658 | mouse_cecum |\n", + "| M4 | PRJEB33889 | ERR3473667 | PRJEB33889 | ERR3473659 | mouse_cecum |\n", + "| M5 | PRJEB33889 | ERR3473668 | PRJEB33889 | ERR3473660 | mouse_cecum |\n", + "| M6 | PRJEB33889 | ERR3473669 | PRJEB33889 | ERR3473661 | mouse_cecum |\n", + "| M7 | PRJEB33889 | ERR3473670 | PRJEB33889 | ERR3473662 | mouse_cecum |\n", + "| M8 | PRJEB33889 | ERR3473671 | PRJEB33889 | ERR3473663 | mouse_cecum |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 M1 PRJEB33889 ERR3473664 PRJEB33889 \n", + "2 M2 PRJEB33889 ERR3473665 PRJEB33889 \n", + "3 M3 PRJEB33889 ERR3473666 PRJEB33889 \n", + "4 M4 PRJEB33889 ERR3473667 PRJEB33889 \n", + "5 M5 PRJEB33889 ERR3473668 PRJEB33889 \n", + "6 M6 PRJEB33889 ERR3473669 PRJEB33889 \n", + "7 M7 PRJEB33889 ERR3473670 PRJEB33889 \n", + "8 M8 PRJEB33889 ERR3473671 PRJEB33889 \n", + " mgx_run_accession sample_type\n", + "1 ERR3473656 mouse_cecum\n", + "2 ERR3473657 mouse_cecum\n", + "3 ERR3473658 mouse_cecum\n", + "4 ERR3473659 mouse_cecum\n", + "5 ERR3473660 mouse_cecum\n", + "6 ERR3473661 mouse_cecum\n", + "7 ERR3473662 mouse_cecum\n", + "8 ERR3473663 mouse_cecum" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjeb33889_mtx <- prjeb33889 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " mutate(sample_name = gsub(\"T\", \"\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjeb33889_mgx <- prjeb33889 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " filter(!grepl(\"16S\", sample_alias)) %>%\n", + " mutate(sample_name = gsub(\"G\", \"\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjeb33889_mtx_vs_mgx <- left_join(prjeb33889_mtx, prjeb33889_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"mouse_cecum\")\n", + "\n", + "prjeb33889_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "f04c78a3", + "metadata": {}, + "source": [ + "## PRJNA698464" + ] + }, + { + "cell_type": "code", + "execution_count": 41, + "id": "7fcee7aa", + "metadata": {}, + "outputs": [], + "source": [ + "prjna698464 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA698464&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 42, + "id": "ccada9e6", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 4 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
ad_supplemented_with_nitrogen_data_2PRJNA698464SRR13618127PRJNA698464SRR13618123bioreactor
ad_supplemented_with_nitrogen_data_1PRJNA698464SRR13618128PRJNA698464SRR13618124bioreactor
ad_supplemented_with_hydrogen_data_2PRJNA698464SRR13618129PRJNA698464SRR13618125bioreactor
ad_supplemented_with_hydrogen_data_1PRJNA698464SRR13618130PRJNA698464SRR13618126bioreactor
\n" + ], + "text/latex": [ + "A tibble: 4 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t ad\\_supplemented\\_with\\_nitrogen\\_data\\_2 & PRJNA698464 & SRR13618127 & PRJNA698464 & SRR13618123 & bioreactor\\\\\n", + "\t ad\\_supplemented\\_with\\_nitrogen\\_data\\_1 & PRJNA698464 & SRR13618128 & PRJNA698464 & SRR13618124 & bioreactor\\\\\n", + "\t ad\\_supplemented\\_with\\_hydrogen\\_data\\_2 & PRJNA698464 & SRR13618129 & PRJNA698464 & SRR13618125 & bioreactor\\\\\n", + "\t ad\\_supplemented\\_with\\_hydrogen\\_data\\_1 & PRJNA698464 & SRR13618130 & PRJNA698464 & SRR13618126 & bioreactor\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 4 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| ad_supplemented_with_nitrogen_data_2 | PRJNA698464 | SRR13618127 | PRJNA698464 | SRR13618123 | bioreactor |\n", + "| ad_supplemented_with_nitrogen_data_1 | PRJNA698464 | SRR13618128 | PRJNA698464 | SRR13618124 | bioreactor |\n", + "| ad_supplemented_with_hydrogen_data_2 | PRJNA698464 | SRR13618129 | PRJNA698464 | SRR13618125 | bioreactor |\n", + "| ad_supplemented_with_hydrogen_data_1 | PRJNA698464 | SRR13618130 | PRJNA698464 | SRR13618126 | bioreactor |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession\n", + "1 ad_supplemented_with_nitrogen_data_2 PRJNA698464 SRR13618127 \n", + "2 ad_supplemented_with_nitrogen_data_1 PRJNA698464 SRR13618128 \n", + "3 ad_supplemented_with_hydrogen_data_2 PRJNA698464 SRR13618129 \n", + "4 ad_supplemented_with_hydrogen_data_1 PRJNA698464 SRR13618130 \n", + " mgx_study_accession mgx_run_accession sample_type\n", + "1 PRJNA698464 SRR13618123 bioreactor \n", + "2 PRJNA698464 SRR13618124 bioreactor \n", + "3 PRJNA698464 SRR13618125 bioreactor \n", + "4 PRJNA698464 SRR13618126 bioreactor " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna698464_mtx <- prjna698464 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " mutate(sample_name = gsub(\"Illumina MiSeq sequencing; \", \"\", experiment_title),\n", + " sample_name = gsub(\"RNA\", \"\", sample_name),\n", + " sample_name = make_clean_names(sample_name)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna698464_mgx <- prjna698464 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " mutate(sample_name = gsub(\"Illumina MiSeq sequencing; \", \"\", experiment_title),\n", + " sample_name = gsub(\"DNA\", \"\", sample_name),\n", + " sample_name = make_clean_names(sample_name)) %>%\n", + " select(sample_name,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna698464_mtx_vs_mgx <- left_join(prjna698464_mtx, prjna698464_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"bioreactor\")\n", + "\n", + "prjna698464_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "e51f3b6d", + "metadata": {}, + "source": [ + "## PRJNA396840" + ] + }, + { + "cell_type": "code", + "execution_count": 43, + "id": "d841dd17", + "metadata": {}, + "outputs": [], + "source": [ + "prjna396840 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA396840&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 44, + "id": "a6864499", + "metadata": { + "scrolled": true + }, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 30 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
X4074PRJNA396840SRR5892181PRJNA396840SRR5892217human_oral
X4080PRJNA396840SRR5892182PRJNA396840SRR5892216human_oral
X4068PRJNA396840SRR5892183PRJNA396840SRR5892215human_oral
X4072PRJNA396840SRR5892184PRJNA396840SRR5892214human_oral
X4060PRJNA396840SRR5892185PRJNA396840SRR5892213human_oral
X4064PRJNA396840SRR5892186PRJNA396840SRR5892212human_oral
X4050PRJNA396840SRR5892187PRJNA396840SRR5892211human_oral
X4056PRJNA396840SRR5892188PRJNA396840SRR5892210human_oral
X4108PRJNA396840SRR5892189PRJNA396840SRR5892233human_oral
X4082PRJNA396840SRR5892190PRJNA396840SRR5892209human_oral
X4088PRJNA396840SRR5892191PRJNA396840SRR5892208human_oral
X4124PRJNA396840SRR5892192PRJNA396840SRR5892236human_oral
X4120PRJNA396840SRR5892195PRJNA396840SRR5892235human_oral
X4096PRJNA396840SRR5892200PRJNA396840SRR5892238human_oral
X4092PRJNA396840SRR5892204PRJNA396840SRR5892237human_oral
X4104PRJNA396840SRR5892205PRJNA396840SRR5892240human_oral
X4100PRJNA396840SRR5892207PRJNA396840SRR5892239human_oral
X4024PRJNA396840SRR5892218PRJNA396840SRR5892196human_oral
X4020PRJNA396840SRR5892219PRJNA396840SRR5892197human_oral
X4014PRJNA396840SRR5892220PRJNA396840SRR5892198human_oral
X4012PRJNA396840SRR5892221PRJNA396840SRR5892199human_oral
X4040PRJNA396840SRR5892222PRJNA396840SRR5892206human_oral
X4036PRJNA396840SRR5892223PRJNA396840SRR5892201human_oral
X4032PRJNA396840SRR5892224PRJNA396840SRR5892202human_oral
X4028PRJNA396840SRR5892225PRJNA396840SRR5892203human_oral
X4048PRJNA396840SRR5892226PRJNA396840SRR5892193human_oral
X4044PRJNA396840SRR5892227PRJNA396840SRR5892194human_oral
X4132PRJNA396840SRR5892228PRJNA396840SRR5892232human_oral
X4128PRJNA396840SRR5892229PRJNA396840SRR5892231human_oral
X4116PRJNA396840SRR5892230PRJNA396840SRR5892234human_oral
\n" + ], + "text/latex": [ + "A tibble: 30 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t X4074 & PRJNA396840 & SRR5892181 & PRJNA396840 & SRR5892217 & human\\_oral\\\\\n", + "\t X4080 & PRJNA396840 & SRR5892182 & PRJNA396840 & SRR5892216 & human\\_oral\\\\\n", + "\t X4068 & PRJNA396840 & SRR5892183 & PRJNA396840 & SRR5892215 & human\\_oral\\\\\n", + "\t X4072 & PRJNA396840 & SRR5892184 & PRJNA396840 & SRR5892214 & human\\_oral\\\\\n", + "\t X4060 & PRJNA396840 & SRR5892185 & PRJNA396840 & SRR5892213 & human\\_oral\\\\\n", + "\t X4064 & PRJNA396840 & SRR5892186 & PRJNA396840 & SRR5892212 & human\\_oral\\\\\n", + "\t X4050 & PRJNA396840 & SRR5892187 & PRJNA396840 & SRR5892211 & human\\_oral\\\\\n", + "\t X4056 & PRJNA396840 & SRR5892188 & PRJNA396840 & SRR5892210 & human\\_oral\\\\\n", + "\t X4108 & PRJNA396840 & SRR5892189 & PRJNA396840 & SRR5892233 & human\\_oral\\\\\n", + "\t X4082 & PRJNA396840 & SRR5892190 & PRJNA396840 & SRR5892209 & human\\_oral\\\\\n", + "\t X4088 & PRJNA396840 & SRR5892191 & PRJNA396840 & SRR5892208 & human\\_oral\\\\\n", + "\t X4124 & PRJNA396840 & SRR5892192 & PRJNA396840 & SRR5892236 & human\\_oral\\\\\n", + "\t X4120 & PRJNA396840 & SRR5892195 & PRJNA396840 & SRR5892235 & human\\_oral\\\\\n", + "\t X4096 & PRJNA396840 & SRR5892200 & PRJNA396840 & SRR5892238 & human\\_oral\\\\\n", + "\t X4092 & PRJNA396840 & SRR5892204 & PRJNA396840 & SRR5892237 & human\\_oral\\\\\n", + "\t X4104 & PRJNA396840 & SRR5892205 & PRJNA396840 & SRR5892240 & human\\_oral\\\\\n", + "\t X4100 & PRJNA396840 & SRR5892207 & PRJNA396840 & SRR5892239 & human\\_oral\\\\\n", + "\t X4024 & PRJNA396840 & SRR5892218 & PRJNA396840 & SRR5892196 & human\\_oral\\\\\n", + "\t X4020 & PRJNA396840 & SRR5892219 & PRJNA396840 & SRR5892197 & human\\_oral\\\\\n", + "\t X4014 & PRJNA396840 & SRR5892220 & PRJNA396840 & SRR5892198 & human\\_oral\\\\\n", + "\t X4012 & PRJNA396840 & SRR5892221 & PRJNA396840 & SRR5892199 & human\\_oral\\\\\n", + "\t X4040 & PRJNA396840 & SRR5892222 & PRJNA396840 & SRR5892206 & human\\_oral\\\\\n", + "\t X4036 & PRJNA396840 & SRR5892223 & PRJNA396840 & SRR5892201 & human\\_oral\\\\\n", + "\t X4032 & PRJNA396840 & SRR5892224 & PRJNA396840 & SRR5892202 & human\\_oral\\\\\n", + "\t X4028 & PRJNA396840 & SRR5892225 & PRJNA396840 & SRR5892203 & human\\_oral\\\\\n", + "\t X4048 & PRJNA396840 & SRR5892226 & PRJNA396840 & SRR5892193 & human\\_oral\\\\\n", + "\t X4044 & PRJNA396840 & SRR5892227 & PRJNA396840 & SRR5892194 & human\\_oral\\\\\n", + "\t X4132 & PRJNA396840 & SRR5892228 & PRJNA396840 & SRR5892232 & human\\_oral\\\\\n", + "\t X4128 & PRJNA396840 & SRR5892229 & PRJNA396840 & SRR5892231 & human\\_oral\\\\\n", + "\t X4116 & PRJNA396840 & SRR5892230 & PRJNA396840 & SRR5892234 & human\\_oral\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 30 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| X4074 | PRJNA396840 | SRR5892181 | PRJNA396840 | SRR5892217 | human_oral |\n", + "| X4080 | PRJNA396840 | SRR5892182 | PRJNA396840 | SRR5892216 | human_oral |\n", + "| X4068 | PRJNA396840 | SRR5892183 | PRJNA396840 | SRR5892215 | human_oral |\n", + "| X4072 | PRJNA396840 | SRR5892184 | PRJNA396840 | SRR5892214 | human_oral |\n", + "| X4060 | PRJNA396840 | SRR5892185 | PRJNA396840 | SRR5892213 | human_oral |\n", + "| X4064 | PRJNA396840 | SRR5892186 | PRJNA396840 | SRR5892212 | human_oral |\n", + "| X4050 | PRJNA396840 | SRR5892187 | PRJNA396840 | SRR5892211 | human_oral |\n", + "| X4056 | PRJNA396840 | SRR5892188 | PRJNA396840 | SRR5892210 | human_oral |\n", + "| X4108 | PRJNA396840 | SRR5892189 | PRJNA396840 | SRR5892233 | human_oral |\n", + "| X4082 | PRJNA396840 | SRR5892190 | PRJNA396840 | SRR5892209 | human_oral |\n", + "| X4088 | PRJNA396840 | SRR5892191 | PRJNA396840 | SRR5892208 | human_oral |\n", + "| X4124 | PRJNA396840 | SRR5892192 | PRJNA396840 | SRR5892236 | human_oral |\n", + "| X4120 | PRJNA396840 | SRR5892195 | PRJNA396840 | SRR5892235 | human_oral |\n", + "| X4096 | PRJNA396840 | SRR5892200 | PRJNA396840 | SRR5892238 | human_oral |\n", + "| X4092 | PRJNA396840 | SRR5892204 | PRJNA396840 | SRR5892237 | human_oral |\n", + "| X4104 | PRJNA396840 | SRR5892205 | PRJNA396840 | SRR5892240 | human_oral |\n", + "| X4100 | PRJNA396840 | SRR5892207 | PRJNA396840 | SRR5892239 | human_oral |\n", + "| X4024 | PRJNA396840 | SRR5892218 | PRJNA396840 | SRR5892196 | human_oral |\n", + "| X4020 | PRJNA396840 | SRR5892219 | PRJNA396840 | SRR5892197 | human_oral |\n", + "| X4014 | PRJNA396840 | SRR5892220 | PRJNA396840 | SRR5892198 | human_oral |\n", + "| X4012 | PRJNA396840 | SRR5892221 | PRJNA396840 | SRR5892199 | human_oral |\n", + "| X4040 | PRJNA396840 | SRR5892222 | PRJNA396840 | SRR5892206 | human_oral |\n", + "| X4036 | PRJNA396840 | SRR5892223 | PRJNA396840 | SRR5892201 | human_oral |\n", + "| X4032 | PRJNA396840 | SRR5892224 | PRJNA396840 | SRR5892202 | human_oral |\n", + "| X4028 | PRJNA396840 | SRR5892225 | PRJNA396840 | SRR5892203 | human_oral |\n", + "| X4048 | PRJNA396840 | SRR5892226 | PRJNA396840 | SRR5892193 | human_oral |\n", + "| X4044 | PRJNA396840 | SRR5892227 | PRJNA396840 | SRR5892194 | human_oral |\n", + "| X4132 | PRJNA396840 | SRR5892228 | PRJNA396840 | SRR5892232 | human_oral |\n", + "| X4128 | PRJNA396840 | SRR5892229 | PRJNA396840 | SRR5892231 | human_oral |\n", + "| X4116 | PRJNA396840 | SRR5892230 | PRJNA396840 | SRR5892234 | human_oral |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 X4074 PRJNA396840 SRR5892181 PRJNA396840 \n", + "2 X4080 PRJNA396840 SRR5892182 PRJNA396840 \n", + "3 X4068 PRJNA396840 SRR5892183 PRJNA396840 \n", + "4 X4072 PRJNA396840 SRR5892184 PRJNA396840 \n", + "5 X4060 PRJNA396840 SRR5892185 PRJNA396840 \n", + "6 X4064 PRJNA396840 SRR5892186 PRJNA396840 \n", + "7 X4050 PRJNA396840 SRR5892187 PRJNA396840 \n", + "8 X4056 PRJNA396840 SRR5892188 PRJNA396840 \n", + "9 X4108 PRJNA396840 SRR5892189 PRJNA396840 \n", + "10 X4082 PRJNA396840 SRR5892190 PRJNA396840 \n", + "11 X4088 PRJNA396840 SRR5892191 PRJNA396840 \n", + "12 X4124 PRJNA396840 SRR5892192 PRJNA396840 \n", + "13 X4120 PRJNA396840 SRR5892195 PRJNA396840 \n", + "14 X4096 PRJNA396840 SRR5892200 PRJNA396840 \n", + "15 X4092 PRJNA396840 SRR5892204 PRJNA396840 \n", + "16 X4104 PRJNA396840 SRR5892205 PRJNA396840 \n", + "17 X4100 PRJNA396840 SRR5892207 PRJNA396840 \n", + "18 X4024 PRJNA396840 SRR5892218 PRJNA396840 \n", + "19 X4020 PRJNA396840 SRR5892219 PRJNA396840 \n", + "20 X4014 PRJNA396840 SRR5892220 PRJNA396840 \n", + "21 X4012 PRJNA396840 SRR5892221 PRJNA396840 \n", + "22 X4040 PRJNA396840 SRR5892222 PRJNA396840 \n", + "23 X4036 PRJNA396840 SRR5892223 PRJNA396840 \n", + "24 X4032 PRJNA396840 SRR5892224 PRJNA396840 \n", + "25 X4028 PRJNA396840 SRR5892225 PRJNA396840 \n", + "26 X4048 PRJNA396840 SRR5892226 PRJNA396840 \n", + "27 X4044 PRJNA396840 SRR5892227 PRJNA396840 \n", + "28 X4132 PRJNA396840 SRR5892228 PRJNA396840 \n", + "29 X4128 PRJNA396840 SRR5892229 PRJNA396840 \n", + "30 X4116 PRJNA396840 SRR5892230 PRJNA396840 \n", + " mgx_run_accession sample_type\n", + "1 SRR5892217 human_oral \n", + "2 SRR5892216 human_oral \n", + "3 SRR5892215 human_oral \n", + "4 SRR5892214 human_oral \n", + "5 SRR5892213 human_oral \n", + "6 SRR5892212 human_oral \n", + "7 SRR5892211 human_oral \n", + "8 SRR5892210 human_oral \n", + "9 SRR5892233 human_oral \n", + "10 SRR5892209 human_oral \n", + "11 SRR5892208 human_oral \n", + "12 SRR5892236 human_oral \n", + "13 SRR5892235 human_oral \n", + "14 SRR5892238 human_oral \n", + "15 SRR5892237 human_oral \n", + "16 SRR5892240 human_oral \n", + "17 SRR5892239 human_oral \n", + "18 SRR5892196 human_oral \n", + "19 SRR5892197 human_oral \n", + "20 SRR5892198 human_oral \n", + "21 SRR5892199 human_oral \n", + "22 SRR5892206 human_oral \n", + "23 SRR5892201 human_oral \n", + "24 SRR5892202 human_oral \n", + "25 SRR5892203 human_oral \n", + "26 SRR5892193 human_oral \n", + "27 SRR5892194 human_oral \n", + "28 SRR5892232 human_oral \n", + "29 SRR5892231 human_oral \n", + "30 SRR5892234 human_oral " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna396840_mtx <- prjna396840 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " mutate(sample_name = gsub(\"RNA\", \"\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna396840_mgx <- prjna396840 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " mutate(sample_name = gsub(\"DNA\", \"\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna396840_mtx_vs_mgx <- left_join(prjna396840_mtx, prjna396840_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"human_oral\")\n", + "\n", + "prjna396840_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "52d148ea", + "metadata": {}, + "source": [ + "## PRJNA492158" + ] + }, + { + "cell_type": "code", + "execution_count": 45, + "id": "f946d7cd", + "metadata": {}, + "outputs": [], + "source": [ + "prjna492158 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA492158&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 46, + "id": "c5490b6a", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 26 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
L2 PRJNA492158SRR7880350PRJNA492158SRR7880198human_gut
L3 PRJNA492158SRR7880351PRJNA492158SRR7880201human_gut
L5 PRJNA492158SRR7880352PRJNA492158SRR7880203human_gut
L6 PRJNA492158SRR7880353PRJNA492158SRR7880202human_gut
L7 PRJNA492158SRR7880354PRJNA492158SRR7880205human_gut
L8 PRJNA492158SRR7880355PRJNA492158SRR7880204human_gut
L9 PRJNA492158SRR7880356PRJNA492158SRR7880207human_gut
L11PRJNA492158SRR7880357PRJNA492158SRR7880227human_gut
L12PRJNA492158SRR7880358PRJNA492158SRR7880226human_gut
O1 PRJNA492158SRR7880359PRJNA492158SRR7880225human_gut
V6 PRJNA492158SRR7880360PRJNA492158SRR7880217human_gut
V5 PRJNA492158SRR7880361PRJNA492158SRR7880216human_gut
V8 PRJNA492158SRR7880362PRJNA492158SRR7880218human_gut
V7 PRJNA492158SRR7880363PRJNA492158SRR7880219human_gut
V11PRJNA492158SRR7880364PRJNA492158SRR7880223human_gut
V9 PRJNA492158SRR7880365PRJNA492158SRR7880197human_gut
O6 PRJNA492158SRR7880366PRJNA492158SRR7880228human_gut
O8 PRJNA492158SRR7880367PRJNA492158SRR7880220human_gut
O9 PRJNA492158SRR7880368PRJNA492158SRR7880210human_gut
O10PRJNA492158SRR7880369PRJNA492158SRR7880211human_gut
O2 PRJNA492158SRR7880370PRJNA492158SRR7880224human_gut
O3 PRJNA492158SRR7880371PRJNA492158SRR7880231human_gut
O4 PRJNA492158SRR7880372PRJNA492158SRR7880230human_gut
O5 PRJNA492158SRR7880373PRJNA492158SRR7880229human_gut
O11PRJNA492158SRR7880374PRJNA492158SRR7880208human_gut
O12PRJNA492158SRR7880375PRJNA492158SRR7880209human_gut
\n" + ], + "text/latex": [ + "A tibble: 26 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t L2 & PRJNA492158 & SRR7880350 & PRJNA492158 & SRR7880198 & human\\_gut\\\\\n", + "\t L3 & PRJNA492158 & SRR7880351 & PRJNA492158 & SRR7880201 & human\\_gut\\\\\n", + "\t L5 & PRJNA492158 & SRR7880352 & PRJNA492158 & SRR7880203 & human\\_gut\\\\\n", + "\t L6 & PRJNA492158 & SRR7880353 & PRJNA492158 & SRR7880202 & human\\_gut\\\\\n", + "\t L7 & PRJNA492158 & SRR7880354 & PRJNA492158 & SRR7880205 & human\\_gut\\\\\n", + "\t L8 & PRJNA492158 & SRR7880355 & PRJNA492158 & SRR7880204 & human\\_gut\\\\\n", + "\t L9 & PRJNA492158 & SRR7880356 & PRJNA492158 & SRR7880207 & human\\_gut\\\\\n", + "\t L11 & PRJNA492158 & SRR7880357 & PRJNA492158 & SRR7880227 & human\\_gut\\\\\n", + "\t L12 & PRJNA492158 & SRR7880358 & PRJNA492158 & SRR7880226 & human\\_gut\\\\\n", + "\t O1 & PRJNA492158 & SRR7880359 & PRJNA492158 & SRR7880225 & human\\_gut\\\\\n", + "\t V6 & PRJNA492158 & SRR7880360 & PRJNA492158 & SRR7880217 & human\\_gut\\\\\n", + "\t V5 & PRJNA492158 & SRR7880361 & PRJNA492158 & SRR7880216 & human\\_gut\\\\\n", + "\t V8 & PRJNA492158 & SRR7880362 & PRJNA492158 & SRR7880218 & human\\_gut\\\\\n", + "\t V7 & PRJNA492158 & SRR7880363 & PRJNA492158 & SRR7880219 & human\\_gut\\\\\n", + "\t V11 & PRJNA492158 & SRR7880364 & PRJNA492158 & SRR7880223 & human\\_gut\\\\\n", + "\t V9 & PRJNA492158 & SRR7880365 & PRJNA492158 & SRR7880197 & human\\_gut\\\\\n", + "\t O6 & PRJNA492158 & SRR7880366 & PRJNA492158 & SRR7880228 & human\\_gut\\\\\n", + "\t O8 & PRJNA492158 & SRR7880367 & PRJNA492158 & SRR7880220 & human\\_gut\\\\\n", + "\t O9 & PRJNA492158 & SRR7880368 & PRJNA492158 & SRR7880210 & human\\_gut\\\\\n", + "\t O10 & PRJNA492158 & SRR7880369 & PRJNA492158 & SRR7880211 & human\\_gut\\\\\n", + "\t O2 & PRJNA492158 & SRR7880370 & PRJNA492158 & SRR7880224 & human\\_gut\\\\\n", + "\t O3 & PRJNA492158 & SRR7880371 & PRJNA492158 & SRR7880231 & human\\_gut\\\\\n", + "\t O4 & PRJNA492158 & SRR7880372 & PRJNA492158 & SRR7880230 & human\\_gut\\\\\n", + "\t O5 & PRJNA492158 & SRR7880373 & PRJNA492158 & SRR7880229 & human\\_gut\\\\\n", + "\t O11 & PRJNA492158 & SRR7880374 & PRJNA492158 & SRR7880208 & human\\_gut\\\\\n", + "\t O12 & PRJNA492158 & SRR7880375 & PRJNA492158 & SRR7880209 & human\\_gut\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 26 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| L2 | PRJNA492158 | SRR7880350 | PRJNA492158 | SRR7880198 | human_gut |\n", + "| L3 | PRJNA492158 | SRR7880351 | PRJNA492158 | SRR7880201 | human_gut |\n", + "| L5 | PRJNA492158 | SRR7880352 | PRJNA492158 | SRR7880203 | human_gut |\n", + "| L6 | PRJNA492158 | SRR7880353 | PRJNA492158 | SRR7880202 | human_gut |\n", + "| L7 | PRJNA492158 | SRR7880354 | PRJNA492158 | SRR7880205 | human_gut |\n", + "| L8 | PRJNA492158 | SRR7880355 | PRJNA492158 | SRR7880204 | human_gut |\n", + "| L9 | PRJNA492158 | SRR7880356 | PRJNA492158 | SRR7880207 | human_gut |\n", + "| L11 | PRJNA492158 | SRR7880357 | PRJNA492158 | SRR7880227 | human_gut |\n", + "| L12 | PRJNA492158 | SRR7880358 | PRJNA492158 | SRR7880226 | human_gut |\n", + "| O1 | PRJNA492158 | SRR7880359 | PRJNA492158 | SRR7880225 | human_gut |\n", + "| V6 | PRJNA492158 | SRR7880360 | PRJNA492158 | SRR7880217 | human_gut |\n", + "| V5 | PRJNA492158 | SRR7880361 | PRJNA492158 | SRR7880216 | human_gut |\n", + "| V8 | PRJNA492158 | SRR7880362 | PRJNA492158 | SRR7880218 | human_gut |\n", + "| V7 | PRJNA492158 | SRR7880363 | PRJNA492158 | SRR7880219 | human_gut |\n", + "| V11 | PRJNA492158 | SRR7880364 | PRJNA492158 | SRR7880223 | human_gut |\n", + "| V9 | PRJNA492158 | SRR7880365 | PRJNA492158 | SRR7880197 | human_gut |\n", + "| O6 | PRJNA492158 | SRR7880366 | PRJNA492158 | SRR7880228 | human_gut |\n", + "| O8 | PRJNA492158 | SRR7880367 | PRJNA492158 | SRR7880220 | human_gut |\n", + "| O9 | PRJNA492158 | SRR7880368 | PRJNA492158 | SRR7880210 | human_gut |\n", + "| O10 | PRJNA492158 | SRR7880369 | PRJNA492158 | SRR7880211 | human_gut |\n", + "| O2 | PRJNA492158 | SRR7880370 | PRJNA492158 | SRR7880224 | human_gut |\n", + "| O3 | PRJNA492158 | SRR7880371 | PRJNA492158 | SRR7880231 | human_gut |\n", + "| O4 | PRJNA492158 | SRR7880372 | PRJNA492158 | SRR7880230 | human_gut |\n", + "| O5 | PRJNA492158 | SRR7880373 | PRJNA492158 | SRR7880229 | human_gut |\n", + "| O11 | PRJNA492158 | SRR7880374 | PRJNA492158 | SRR7880208 | human_gut |\n", + "| O12 | PRJNA492158 | SRR7880375 | PRJNA492158 | SRR7880209 | human_gut |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 L2 PRJNA492158 SRR7880350 PRJNA492158 \n", + "2 L3 PRJNA492158 SRR7880351 PRJNA492158 \n", + "3 L5 PRJNA492158 SRR7880352 PRJNA492158 \n", + "4 L6 PRJNA492158 SRR7880353 PRJNA492158 \n", + "5 L7 PRJNA492158 SRR7880354 PRJNA492158 \n", + "6 L8 PRJNA492158 SRR7880355 PRJNA492158 \n", + "7 L9 PRJNA492158 SRR7880356 PRJNA492158 \n", + "8 L11 PRJNA492158 SRR7880357 PRJNA492158 \n", + "9 L12 PRJNA492158 SRR7880358 PRJNA492158 \n", + "10 O1 PRJNA492158 SRR7880359 PRJNA492158 \n", + "11 V6 PRJNA492158 SRR7880360 PRJNA492158 \n", + "12 V5 PRJNA492158 SRR7880361 PRJNA492158 \n", + "13 V8 PRJNA492158 SRR7880362 PRJNA492158 \n", + "14 V7 PRJNA492158 SRR7880363 PRJNA492158 \n", + "15 V11 PRJNA492158 SRR7880364 PRJNA492158 \n", + "16 V9 PRJNA492158 SRR7880365 PRJNA492158 \n", + "17 O6 PRJNA492158 SRR7880366 PRJNA492158 \n", + "18 O8 PRJNA492158 SRR7880367 PRJNA492158 \n", + "19 O9 PRJNA492158 SRR7880368 PRJNA492158 \n", + "20 O10 PRJNA492158 SRR7880369 PRJNA492158 \n", + "21 O2 PRJNA492158 SRR7880370 PRJNA492158 \n", + "22 O3 PRJNA492158 SRR7880371 PRJNA492158 \n", + "23 O4 PRJNA492158 SRR7880372 PRJNA492158 \n", + "24 O5 PRJNA492158 SRR7880373 PRJNA492158 \n", + "25 O11 PRJNA492158 SRR7880374 PRJNA492158 \n", + "26 O12 PRJNA492158 SRR7880375 PRJNA492158 \n", + " mgx_run_accession sample_type\n", + "1 SRR7880198 human_gut \n", + "2 SRR7880201 human_gut \n", + "3 SRR7880203 human_gut \n", + "4 SRR7880202 human_gut \n", + "5 SRR7880205 human_gut \n", + "6 SRR7880204 human_gut \n", + "7 SRR7880207 human_gut \n", + "8 SRR7880227 human_gut \n", + "9 SRR7880226 human_gut \n", + "10 SRR7880225 human_gut \n", + "11 SRR7880217 human_gut \n", + "12 SRR7880216 human_gut \n", + "13 SRR7880218 human_gut \n", + "14 SRR7880219 human_gut \n", + "15 SRR7880223 human_gut \n", + "16 SRR7880197 human_gut \n", + "17 SRR7880228 human_gut \n", + "18 SRR7880220 human_gut \n", + "19 SRR7880210 human_gut \n", + "20 SRR7880211 human_gut \n", + "21 SRR7880224 human_gut \n", + "22 SRR7880231 human_gut \n", + "23 SRR7880230 human_gut \n", + "24 SRR7880229 human_gut \n", + "25 SRR7880208 human_gut \n", + "26 SRR7880209 human_gut " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna492158_mtx <- prjna492158 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna492158_mgx <- prjna492158 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna492158_mtx_vs_mgx <- left_join(prjna492158_mtx, prjna492158_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"human_gut\")\n", + "\n", + "prjna492158_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "c6297be8", + "metadata": {}, + "source": [ + "## PRJNA278075" + ] + }, + { + "cell_type": "code", + "execution_count": 47, + "id": "e2bbc1d3", + "metadata": {}, + "outputs": [], + "source": [ + "prjna278075 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA278075&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 48, + "id": "c04f4678", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\n", + "
A tibble: 1 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
3m_Station6_GOMPRJNA278075SRR1918203PRJNA278075SRR2001210ocean
\n" + ], + "text/latex": [ + "A tibble: 1 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t 3m\\_Station6\\_GOM & PRJNA278075 & SRR1918203 & PRJNA278075 & SRR2001210 & ocean\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 1 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| 3m_Station6_GOM | PRJNA278075 | SRR1918203 | PRJNA278075 | SRR2001210 | ocean |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 3m_Station6_GOM PRJNA278075 SRR1918203 PRJNA278075 \n", + " mgx_run_accession sample_type\n", + "1 SRR2001210 ocean " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna278075_mtx <- prjna278075 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " mutate(sample_name = gsub(\"_Metatranscriptome\", \"\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna278075_mgx <- prjna278075 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " mutate(sample_name = gsub(\"_Metagenome\", \"\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna278075_mtx_vs_mgx <- left_join(prjna278075_mtx, prjna278075_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"ocean\")\n", + "\n", + "prjna278075_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "47b51151", + "metadata": {}, + "source": [ + "## PRJEB38017" + ] + }, + { + "cell_type": "code", + "execution_count": 49, + "id": "2ffa1577", + "metadata": {}, + "outputs": [], + "source": [ + "prjeb38017 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJEB38017&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 50, + "id": "de8f831d", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 3 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
CRF2_7 PRJEB38017ERR4077213PRJEB38017ERR4073751cocoa_box_fermentation
CRF2_20PRJEB38017ERR4077214PRJEB38017ERR4073752cocoa_box_fermentation
CRF2_68PRJEB38017ERR4077215PRJEB38017ERR4073754cocoa_box_fermentation
\n" + ], + "text/latex": [ + "A tibble: 3 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t CRF2\\_7 & PRJEB38017 & ERR4077213 & PRJEB38017 & ERR4073751 & cocoa\\_box\\_fermentation\\\\\n", + "\t CRF2\\_20 & PRJEB38017 & ERR4077214 & PRJEB38017 & ERR4073752 & cocoa\\_box\\_fermentation\\\\\n", + "\t CRF2\\_68 & PRJEB38017 & ERR4077215 & PRJEB38017 & ERR4073754 & cocoa\\_box\\_fermentation\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 3 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| CRF2_7 | PRJEB38017 | ERR4077213 | PRJEB38017 | ERR4073751 | cocoa_box_fermentation |\n", + "| CRF2_20 | PRJEB38017 | ERR4077214 | PRJEB38017 | ERR4073752 | cocoa_box_fermentation |\n", + "| CRF2_68 | PRJEB38017 | ERR4077215 | PRJEB38017 | ERR4073754 | cocoa_box_fermentation |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 CRF2_7 PRJEB38017 ERR4077213 PRJEB38017 \n", + "2 CRF2_20 PRJEB38017 ERR4077214 PRJEB38017 \n", + "3 CRF2_68 PRJEB38017 ERR4077215 PRJEB38017 \n", + " mgx_run_accession sample_type \n", + "1 ERR4073751 cocoa_box_fermentation\n", + "2 ERR4073752 cocoa_box_fermentation\n", + "3 ERR4073754 cocoa_box_fermentation" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjeb38017_mtx <- prjeb38017 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " mutate(sample_name = gsub(\"CRF\", \"\", sample_alias),\n", + " sample_name = gsub(\"R\", \"_\", sample_name),\n", + " sample_name = paste0(\"CRF\", sample_name)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjeb38017_mgx <- prjeb38017 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " mutate(sample_name = gsub(\"D\", \"_\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjeb38017_mtx_vs_mgx <- left_join(prjeb38017_mtx, prjeb38017_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"cocoa_box_fermentation\")\n", + "\n", + "prjeb38017_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "f35357e1", + "metadata": {}, + "source": [ + "## PRJNA616041" + ] + }, + { + "cell_type": "code", + "execution_count": 51, + "id": "3fe05289", + "metadata": {}, + "outputs": [], + "source": [ + "prjna616041 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA616041&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 52, + "id": "35e3b96e", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 6 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
HP_AsLow PRJNA616041SRR11450577PRJNA616041SRR11450583paddy_soil
SKS_AsHigPRJNA616041SRR11450578PRJNA616041SRR11450584paddy_soil
CZ_AsHig PRJNA616041SRR11450579PRJNA616041SRR11450587paddy_soil
CL_AsHig PRJNA616041SRR11450580PRJNA616041SRR11450588paddy_soil
YCP_AsLowPRJNA616041SRR11450585PRJNA616041SRR11450581paddy_soil
LH_AsLow PRJNA616041SRR11450586PRJNA616041SRR11450582paddy_soil
\n" + ], + "text/latex": [ + "A tibble: 6 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t HP\\_AsLow & PRJNA616041 & SRR11450577 & PRJNA616041 & SRR11450583 & paddy\\_soil\\\\\n", + "\t SKS\\_AsHig & PRJNA616041 & SRR11450578 & PRJNA616041 & SRR11450584 & paddy\\_soil\\\\\n", + "\t CZ\\_AsHig & PRJNA616041 & SRR11450579 & PRJNA616041 & SRR11450587 & paddy\\_soil\\\\\n", + "\t CL\\_AsHig & PRJNA616041 & SRR11450580 & PRJNA616041 & SRR11450588 & paddy\\_soil\\\\\n", + "\t YCP\\_AsLow & PRJNA616041 & SRR11450585 & PRJNA616041 & SRR11450581 & paddy\\_soil\\\\\n", + "\t LH\\_AsLow & PRJNA616041 & SRR11450586 & PRJNA616041 & SRR11450582 & paddy\\_soil\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 6 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| HP_AsLow | PRJNA616041 | SRR11450577 | PRJNA616041 | SRR11450583 | paddy_soil |\n", + "| SKS_AsHig | PRJNA616041 | SRR11450578 | PRJNA616041 | SRR11450584 | paddy_soil |\n", + "| CZ_AsHig | PRJNA616041 | SRR11450579 | PRJNA616041 | SRR11450587 | paddy_soil |\n", + "| CL_AsHig | PRJNA616041 | SRR11450580 | PRJNA616041 | SRR11450588 | paddy_soil |\n", + "| YCP_AsLow | PRJNA616041 | SRR11450585 | PRJNA616041 | SRR11450581 | paddy_soil |\n", + "| LH_AsLow | PRJNA616041 | SRR11450586 | PRJNA616041 | SRR11450582 | paddy_soil |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 HP_AsLow PRJNA616041 SRR11450577 PRJNA616041 \n", + "2 SKS_AsHig PRJNA616041 SRR11450578 PRJNA616041 \n", + "3 CZ_AsHig PRJNA616041 SRR11450579 PRJNA616041 \n", + "4 CL_AsHig PRJNA616041 SRR11450580 PRJNA616041 \n", + "5 YCP_AsLow PRJNA616041 SRR11450585 PRJNA616041 \n", + "6 LH_AsLow PRJNA616041 SRR11450586 PRJNA616041 \n", + " mgx_run_accession sample_type\n", + "1 SRR11450583 paddy_soil \n", + "2 SRR11450584 paddy_soil \n", + "3 SRR11450587 paddy_soil \n", + "4 SRR11450588 paddy_soil \n", + "5 SRR11450581 paddy_soil \n", + "6 SRR11450582 paddy_soil " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna616041_mtx <- prjna616041 %>% \n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna616041_mgx <- prjna616041 %>% \n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna616041_mtx_vs_mgx <- left_join(prjna616041_mtx, prjna616041_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"paddy_soil\")\n", + "\n", + "prjna616041_mtx_vs_mgx" + ] + }, + { + "cell_type": "markdown", + "id": "d3351487", + "metadata": {}, + "source": [ + "## PRJNA393770 (mgx) & PRJNA395125 (mtx)" + ] + }, + { + "cell_type": "code", + "execution_count": 53, + "id": "5b193bc9", + "metadata": {}, + "outputs": [], + "source": [ + "prjna393770 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA393770&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)\n", + "\n", + "prjna395125 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA395125&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 54, + "id": "b7dfdb12", + "metadata": {}, + "outputs": [], + "source": [ + "prjna393770_mgx <- prjna393770 %>%\n", + " filter(library_source %in% c(\"METAGENOMIC\")) %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna395125_mtx <- prjna395125 %>%\n", + " filter(library_source %in% c(\"METATRANSCRIPTOMIC\")) %>%\n", + " mutate(sample_name = gsub(\"_MT\", \"\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna395125_vs_prjna393770 <- inner_join(prjna395125_mtx, prjna393770_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"bioreactor\")" + ] + }, + { + "cell_type": "markdown", + "id": "718078bd", + "metadata": {}, + "source": [ + "## PRJEB12284 (mtx) & PRJEB12083 (mgx)" + ] + }, + { + "cell_type": "code", + "execution_count": 55, + "id": "3c808614", + "metadata": {}, + "outputs": [], + "source": [ + "prjeb12284 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJEB12284&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)\n", + "\n", + "prjeb12083 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJEB12083&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 56, + "id": "2dfe7df8", + "metadata": { + "scrolled": true + }, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 8 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
AH1PRJEB12284ERR1198915PRJEB12083ERR1191817wastewater
AH4PRJEB12284ERR1198916PRJEB12083ERR1191820wastewater
AH5PRJEB12284ERR1198917PRJEB12083ERR1191821wastewater
AH6PRJEB12284ERR1198918PRJEB12083ERR1191822wastewater
DF1PRJEB12284ERR1198919PRJEB12083ERR1193331wastewater
DF4PRJEB12284ERR1198920PRJEB12083ERR1193299wastewater
DF5PRJEB12284ERR1198921PRJEB12083ERR1193300wastewater
DF6PRJEB12284ERR1198922PRJEB12083ERR1193301wastewater
\n" + ], + "text/latex": [ + "A tibble: 8 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t AH1 & PRJEB12284 & ERR1198915 & PRJEB12083 & ERR1191817 & wastewater\\\\\n", + "\t AH4 & PRJEB12284 & ERR1198916 & PRJEB12083 & ERR1191820 & wastewater\\\\\n", + "\t AH5 & PRJEB12284 & ERR1198917 & PRJEB12083 & ERR1191821 & wastewater\\\\\n", + "\t AH6 & PRJEB12284 & ERR1198918 & PRJEB12083 & ERR1191822 & wastewater\\\\\n", + "\t DF1 & PRJEB12284 & ERR1198919 & PRJEB12083 & ERR1193331 & wastewater\\\\\n", + "\t DF4 & PRJEB12284 & ERR1198920 & PRJEB12083 & ERR1193299 & wastewater\\\\\n", + "\t DF5 & PRJEB12284 & ERR1198921 & PRJEB12083 & ERR1193300 & wastewater\\\\\n", + "\t DF6 & PRJEB12284 & ERR1198922 & PRJEB12083 & ERR1193301 & wastewater\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 8 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| AH1 | PRJEB12284 | ERR1198915 | PRJEB12083 | ERR1191817 | wastewater |\n", + "| AH4 | PRJEB12284 | ERR1198916 | PRJEB12083 | ERR1191820 | wastewater |\n", + "| AH5 | PRJEB12284 | ERR1198917 | PRJEB12083 | ERR1191821 | wastewater |\n", + "| AH6 | PRJEB12284 | ERR1198918 | PRJEB12083 | ERR1191822 | wastewater |\n", + "| DF1 | PRJEB12284 | ERR1198919 | PRJEB12083 | ERR1193331 | wastewater |\n", + "| DF4 | PRJEB12284 | ERR1198920 | PRJEB12083 | ERR1193299 | wastewater |\n", + "| DF5 | PRJEB12284 | ERR1198921 | PRJEB12083 | ERR1193300 | wastewater |\n", + "| DF6 | PRJEB12284 | ERR1198922 | PRJEB12083 | ERR1193301 | wastewater |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 AH1 PRJEB12284 ERR1198915 PRJEB12083 \n", + "2 AH4 PRJEB12284 ERR1198916 PRJEB12083 \n", + "3 AH5 PRJEB12284 ERR1198917 PRJEB12083 \n", + "4 AH6 PRJEB12284 ERR1198918 PRJEB12083 \n", + "5 DF1 PRJEB12284 ERR1198919 PRJEB12083 \n", + "6 DF4 PRJEB12284 ERR1198920 PRJEB12083 \n", + "7 DF5 PRJEB12284 ERR1198921 PRJEB12083 \n", + "8 DF6 PRJEB12284 ERR1198922 PRJEB12083 \n", + " mgx_run_accession sample_type\n", + "1 ERR1191817 wastewater \n", + "2 ERR1191820 wastewater \n", + "3 ERR1191821 wastewater \n", + "4 ERR1191822 wastewater \n", + "5 ERR1193331 wastewater \n", + "6 ERR1193299 wastewater \n", + "7 ERR1193300 wastewater \n", + "8 ERR1193301 wastewater " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjeb12284_mtx <- prjeb12284 %>%\n", + " mutate(sample_name = gsub(\"\\\\.\", \"\", sample_alias)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjeb12083_mgx <- prjeb12083 %>%\n", + " select(sample_name = sample_alias,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjeb12284_vs_prjeb12083 <- inner_join(prjeb12284_mtx, prjeb12083_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"wastewater\")\n", + "\n", + "prjeb12284_vs_prjeb12083" + ] + }, + { + "cell_type": "markdown", + "id": "256aec2e", + "metadata": {}, + "source": [ + "## PRJEB32787 (mgx) & PRJEB32788 (mtx)" + ] + }, + { + "cell_type": "code", + "execution_count": 57, + "id": "6041c9ec", + "metadata": {}, + "outputs": [], + "source": [ + "prjeb32787 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJEB32787&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)\n", + "\n", + "prjeb32788 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJEB32788&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 58, + "id": "c46f0f14", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 17 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
BazoSym_LS-ET_5PRJEB32788ERR3342479PRJEB32787ERR3342499mussel_gill
BazoSym_LS-ET_4PRJEB32788ERR3342480PRJEB32787ERR3342500mussel_gill
BazoSym_LS-MS_3PRJEB32788ERR3342481PRJEB32787ERR3342501mussel_gill
BazoSym_LS-MS_2PRJEB32788ERR3342482PRJEB32787ERR3342502mussel_gill
BazoSym_LS-MS_1PRJEB32788ERR3342483PRJEB32787ERR3342503mussel_gill
BspSym_Li_5 PRJEB32788ERR3342467PRJEB32787ERR3342486mussel_gill
BspSym_Li_4 PRJEB32788ERR3342468PRJEB32787ERR3342487mussel_gill
BspSym_Li_3 PRJEB32788ERR3342469PRJEB32787ERR3342488mussel_gill
BspSym_Li_2 PRJEB32788ERR3342470PRJEB32787ERR3342489mussel_gill
BspSym_Li_1 PRJEB32788ERR3342471PRJEB32787ERR3342490mussel_gill
BspSym_Cl_4 PRJEB32788ERR3342472PRJEB32787ERR3342492mussel_gill
BspSym_Cl_3 PRJEB32788ERR3342473PRJEB32787ERR3342493mussel_gill
BspSym_Cl_2 PRJEB32788ERR3342474PRJEB32787ERR3342494mussel_gill
BspSym_Cl_1 PRJEB32788ERR3342475PRJEB32787ERR3342495mussel_gill
BputSym_Se-AL_3PRJEB32788ERR3342476PRJEB32787ERR3342496mussel_gill
BputSym_Se-AL_2PRJEB32788ERR3342477PRJEB32787ERR3342497mussel_gill
BputSym_Se-AL_1PRJEB32788ERR3342478PRJEB32787ERR3342498mussel_gill
\n" + ], + "text/latex": [ + "A tibble: 17 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t BazoSym\\_LS-ET\\_5 & PRJEB32788 & ERR3342479 & PRJEB32787 & ERR3342499 & mussel\\_gill\\\\\n", + "\t BazoSym\\_LS-ET\\_4 & PRJEB32788 & ERR3342480 & PRJEB32787 & ERR3342500 & mussel\\_gill\\\\\n", + "\t BazoSym\\_LS-MS\\_3 & PRJEB32788 & ERR3342481 & PRJEB32787 & ERR3342501 & mussel\\_gill\\\\\n", + "\t BazoSym\\_LS-MS\\_2 & PRJEB32788 & ERR3342482 & PRJEB32787 & ERR3342502 & mussel\\_gill\\\\\n", + "\t BazoSym\\_LS-MS\\_1 & PRJEB32788 & ERR3342483 & PRJEB32787 & ERR3342503 & mussel\\_gill\\\\\n", + "\t BspSym\\_Li\\_5 & PRJEB32788 & ERR3342467 & PRJEB32787 & ERR3342486 & mussel\\_gill\\\\\n", + "\t BspSym\\_Li\\_4 & PRJEB32788 & ERR3342468 & PRJEB32787 & ERR3342487 & mussel\\_gill\\\\\n", + "\t BspSym\\_Li\\_3 & PRJEB32788 & ERR3342469 & PRJEB32787 & ERR3342488 & mussel\\_gill\\\\\n", + "\t BspSym\\_Li\\_2 & PRJEB32788 & ERR3342470 & PRJEB32787 & ERR3342489 & mussel\\_gill\\\\\n", + "\t BspSym\\_Li\\_1 & PRJEB32788 & ERR3342471 & PRJEB32787 & ERR3342490 & mussel\\_gill\\\\\n", + "\t BspSym\\_Cl\\_4 & PRJEB32788 & ERR3342472 & PRJEB32787 & ERR3342492 & mussel\\_gill\\\\\n", + "\t BspSym\\_Cl\\_3 & PRJEB32788 & ERR3342473 & PRJEB32787 & ERR3342493 & mussel\\_gill\\\\\n", + "\t BspSym\\_Cl\\_2 & PRJEB32788 & ERR3342474 & PRJEB32787 & ERR3342494 & mussel\\_gill\\\\\n", + "\t BspSym\\_Cl\\_1 & PRJEB32788 & ERR3342475 & PRJEB32787 & ERR3342495 & mussel\\_gill\\\\\n", + "\t BputSym\\_Se-AL\\_3 & PRJEB32788 & ERR3342476 & PRJEB32787 & ERR3342496 & mussel\\_gill\\\\\n", + "\t BputSym\\_Se-AL\\_2 & PRJEB32788 & ERR3342477 & PRJEB32787 & ERR3342497 & mussel\\_gill\\\\\n", + "\t BputSym\\_Se-AL\\_1 & PRJEB32788 & ERR3342478 & PRJEB32787 & ERR3342498 & mussel\\_gill\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 17 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| BazoSym_LS-ET_5 | PRJEB32788 | ERR3342479 | PRJEB32787 | ERR3342499 | mussel_gill |\n", + "| BazoSym_LS-ET_4 | PRJEB32788 | ERR3342480 | PRJEB32787 | ERR3342500 | mussel_gill |\n", + "| BazoSym_LS-MS_3 | PRJEB32788 | ERR3342481 | PRJEB32787 | ERR3342501 | mussel_gill |\n", + "| BazoSym_LS-MS_2 | PRJEB32788 | ERR3342482 | PRJEB32787 | ERR3342502 | mussel_gill |\n", + "| BazoSym_LS-MS_1 | PRJEB32788 | ERR3342483 | PRJEB32787 | ERR3342503 | mussel_gill |\n", + "| BspSym_Li_5 | PRJEB32788 | ERR3342467 | PRJEB32787 | ERR3342486 | mussel_gill |\n", + "| BspSym_Li_4 | PRJEB32788 | ERR3342468 | PRJEB32787 | ERR3342487 | mussel_gill |\n", + "| BspSym_Li_3 | PRJEB32788 | ERR3342469 | PRJEB32787 | ERR3342488 | mussel_gill |\n", + "| BspSym_Li_2 | PRJEB32788 | ERR3342470 | PRJEB32787 | ERR3342489 | mussel_gill |\n", + "| BspSym_Li_1 | PRJEB32788 | ERR3342471 | PRJEB32787 | ERR3342490 | mussel_gill |\n", + "| BspSym_Cl_4 | PRJEB32788 | ERR3342472 | PRJEB32787 | ERR3342492 | mussel_gill |\n", + "| BspSym_Cl_3 | PRJEB32788 | ERR3342473 | PRJEB32787 | ERR3342493 | mussel_gill |\n", + "| BspSym_Cl_2 | PRJEB32788 | ERR3342474 | PRJEB32787 | ERR3342494 | mussel_gill |\n", + "| BspSym_Cl_1 | PRJEB32788 | ERR3342475 | PRJEB32787 | ERR3342495 | mussel_gill |\n", + "| BputSym_Se-AL_3 | PRJEB32788 | ERR3342476 | PRJEB32787 | ERR3342496 | mussel_gill |\n", + "| BputSym_Se-AL_2 | PRJEB32788 | ERR3342477 | PRJEB32787 | ERR3342497 | mussel_gill |\n", + "| BputSym_Se-AL_1 | PRJEB32788 | ERR3342478 | PRJEB32787 | ERR3342498 | mussel_gill |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 BazoSym_LS-ET_5 PRJEB32788 ERR3342479 PRJEB32787 \n", + "2 BazoSym_LS-ET_4 PRJEB32788 ERR3342480 PRJEB32787 \n", + "3 BazoSym_LS-MS_3 PRJEB32788 ERR3342481 PRJEB32787 \n", + "4 BazoSym_LS-MS_2 PRJEB32788 ERR3342482 PRJEB32787 \n", + "5 BazoSym_LS-MS_1 PRJEB32788 ERR3342483 PRJEB32787 \n", + "6 BspSym_Li_5 PRJEB32788 ERR3342467 PRJEB32787 \n", + "7 BspSym_Li_4 PRJEB32788 ERR3342468 PRJEB32787 \n", + "8 BspSym_Li_3 PRJEB32788 ERR3342469 PRJEB32787 \n", + "9 BspSym_Li_2 PRJEB32788 ERR3342470 PRJEB32787 \n", + "10 BspSym_Li_1 PRJEB32788 ERR3342471 PRJEB32787 \n", + "11 BspSym_Cl_4 PRJEB32788 ERR3342472 PRJEB32787 \n", + "12 BspSym_Cl_3 PRJEB32788 ERR3342473 PRJEB32787 \n", + "13 BspSym_Cl_2 PRJEB32788 ERR3342474 PRJEB32787 \n", + "14 BspSym_Cl_1 PRJEB32788 ERR3342475 PRJEB32787 \n", + "15 BputSym_Se-AL_3 PRJEB32788 ERR3342476 PRJEB32787 \n", + "16 BputSym_Se-AL_2 PRJEB32788 ERR3342477 PRJEB32787 \n", + "17 BputSym_Se-AL_1 PRJEB32788 ERR3342478 PRJEB32787 \n", + " mgx_run_accession sample_type\n", + "1 ERR3342499 mussel_gill\n", + "2 ERR3342500 mussel_gill\n", + "3 ERR3342501 mussel_gill\n", + "4 ERR3342502 mussel_gill\n", + "5 ERR3342503 mussel_gill\n", + "6 ERR3342486 mussel_gill\n", + "7 ERR3342487 mussel_gill\n", + "8 ERR3342488 mussel_gill\n", + "9 ERR3342489 mussel_gill\n", + "10 ERR3342490 mussel_gill\n", + "11 ERR3342492 mussel_gill\n", + "12 ERR3342493 mussel_gill\n", + "13 ERR3342494 mussel_gill\n", + "14 ERR3342495 mussel_gill\n", + "15 ERR3342496 mussel_gill\n", + "16 ERR3342497 mussel_gill\n", + "17 ERR3342498 mussel_gill" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjeb32787_mgx <- prjeb32787 %>%\n", + " mutate(sample_name = gsub(\"_[^_]*$\", \"\", sample_title)) %>%\n", + " select(sample_name,\n", + " mgx_study_accession = study_accession,\n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjeb32788_mtx <- prjeb32788 %>%\n", + " mutate(sample_name = gsub(\"_[^_]*$\", \"\", sample_title)) %>%\n", + " select(sample_name,\n", + " mtx_study_accession = study_accession,\n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjeb32788_vs_prjeb32781_1 <- left_join(prjeb32788_mtx, prjeb32787_mgx, by = \"sample_name\") %>%\n", + " filter(!is.na(mgx_study_accession))\n", + "\n", + "prjeb32788_mtx_2 <- prjeb32788_mtx %>%\n", + " mutate(sample_name = gsub(\"_[^_]*$\", \"\", sample_name)) \n", + "\n", + "prjeb32788_vs_prjeb32781_2 <- left_join(prjeb32788_mtx_2, prjeb32787_mgx, by = \"sample_name\") %>%\n", + " filter(!is.na(mgx_study_accession))\n", + "\n", + "prjeb32788_vs_prjeb32781 <- bind_rows(prjeb32788_vs_prjeb32781_1, prjeb32788_vs_prjeb32781_2) %>%\n", + " mutate(sample_type = \"mussel_gill\")\n", + "\n", + "prjeb32788_vs_prjeb32781 " + ] + }, + { + "cell_type": "markdown", + "id": "cd95fc8c", + "metadata": {}, + "source": [ + "## iHMP (PRJNA398089)" + ] + }, + { + "cell_type": "code", + "execution_count": 59, + "id": "1f0ccf12", + "metadata": {}, + "outputs": [], + "source": [ + "prjna398089 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA398089&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 60, + "id": "dd826c94", + "metadata": { + "scrolled": true + }, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 761 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
CSM67UC6PRJNA398089SRR5949109PRJNA398089SRR5936217human_gut
CSM67UDYPRJNA398089SRR5949110PRJNA398089SRR5936216human_gut
HSM67VI9PRJNA398089SRR5949111PRJNA398089SRR5936212human_gut
HSM6XRTQPRJNA398089SRR5949112PRJNA398089SRR5936211human_gut
CSM79HHMPRJNA398089SRR5949113PRJNA398089SRR5946811human_gut
CSM79HIRPRJNA398089SRR5949114PRJNA398089SRR5936215human_gut
CSM79HJOPRJNA398089SRR5949115PRJNA398089SRR5947089human_gut
HSM67VEIPRJNA398089SRR5949116PRJNA398089SRR5936210human_gut
MSM79H9KPRJNA398089SRR5949117PRJNA398089SRR5935975human_gut
MSM79HAHPRJNA398089SRR5949118PRJNA398089SRR5935976human_gut
HSM7J4L5PRJNA398089SRR5949119PRJNA398089SRR5935953human_gut
MSM79H7EPRJNA398089SRR5949120PRJNA398089SRR5935950human_gut
PSM7J17ZPRJNA398089SRR5949121PRJNA398089SRR5935951human_gut
PSM7J18GPRJNA398089SRR5949122PRJNA398089SRR5935952human_gut
MSM79HDIPRJNA398089SRR5949123PRJNA398089SRR5935954human_gut
HSM7J4JHPRJNA398089SRR5949124PRJNA398089SRR5935955human_gut
HSM7J4HUPRJNA398089SRR5949125PRJNA398089SRR5935956human_gut
PSM7J1CIPRJNA398089SRR5949126PRJNA398089SRR5935957human_gut
HSM7CZ36PRJNA398089SRR5949127PRJNA398089SRR5936016human_gut
CSM7KOJWPRJNA398089SRR5949128PRJNA398089SRR5947102human_gut
MSM79HDMPRJNA398089SRR5949129PRJNA398089SRR5936018human_gut
PSM6XBTPPRJNA398089SRR5949130PRJNA398089SRR5936066human_gut
MSM79HF9PRJNA398089SRR5949131PRJNA398089SRR5936064human_gut
CSM79HQFPRJNA398089SRR5949132PRJNA398089SRR5936119human_gut
MSM79H9APRJNA398089SRR5949133PRJNA398089SRR5936118human_gut
HSM7CZ3EPRJNA398089SRR5949134PRJNA398089SRR5936121human_gut
CSM79HP4PRJNA398089SRR5949135PRJNA398089SRR5946645human_gut
HSM7CYY7PRJNA398089SRR5949136PRJNA398089SRR5936117human_gut
CSM79HLCPRJNA398089SRR5949137PRJNA398089SRR5936176human_gut
MSM79H5KPRJNA398089SRR5949138PRJNA398089SRR5936132human_gut
PSM6XBSM PRJNA398089SRR5963918PRJNA398089SRR5950713human_gut
MSM79HDQ_TRPRJNA398089SRR5963920PRJNA398089SRR5962901human_gut
MSM79HDG_TRPRJNA398089SRR5963921PRJNA398089SRR5962900human_gut
MSM9VZEK_TRPRJNA398089SRR5963922PRJNA398089SRR5962903human_gut
MSM9VZMA_TRPRJNA398089SRR5963923PRJNA398089SRR5962902human_gut
PSMA265J_TRPRJNA398089SRR5963924PRJNA398089SRR5962904human_gut
MSMA26AZ_TRPRJNA398089SRR5963925PRJNA398089SRR5962908human_gut
HSMA33LH_TRPRJNA398089SRR5963926PRJNA398089SRR5962907human_gut
MSM9VZNH_TRPRJNA398089SRR5963927PRJNA398089SRR5962906human_gut
HSMA33RX_TRPRJNA398089SRR5963928PRJNA398089SRR5962885human_gut
PSM6XBQY_TRPRJNA398089SRR5963929PRJNA398089SRR5962895human_gut
CSM79HLA_TRPRJNA398089SRR5963930PRJNA398089SRR5962894human_gut
HSM6XRVC_TRPRJNA398089SRR5963931PRJNA398089SRR5962893human_gut
HSM5MD6A_TRPRJNA398089SRR5963932PRJNA398089SRR5962891human_gut
PSM6XBRK_TRPRJNA398089SRR5963933PRJNA398089SRR5962890human_gut
CSM67UEW_TRPRJNA398089SRR5963934PRJNA398089SRR5962889human_gut
HSM67VEM_TRPRJNA398089SRR5963935PRJNA398089SRR5962892human_gut
CSM67UDR_TRPRJNA398089SRR5963936PRJNA398089SRR5962888human_gut
PSM6XBSU_TRPRJNA398089SRR5963937PRJNA398089SRR5962897human_gut
MSM79HF9_TRPRJNA398089SRR5963938PRJNA398089SRR5962898human_gut
PSM6XBRK PRJNA398089SRR5963939PRJNA398089SRR5935960human_gut
PSM6XBQY PRJNA398089SRR5963940PRJNA398089SRR5935809human_gut
MSMA26EP PRJNA398089SRR5963941PRJNA398089SRR5946720human_gut
MSM9VZOW PRJNA398089SRR5963942PRJNA398089SRR5946733human_gut
PSM6XBQU PRJNA398089SRR5963943PRJNA398089SRR5936007human_gut
MSMAPC5Z PRJNA398089SRR5963944PRJNA398089SRR5946832human_gut
MSM6J2M3 PRJNA398089SRR5963945PRJNA398089SRR5935963human_gut
MSM6J2K6 PRJNA398089SRR5963946PRJNA398089SRR5935938human_gut
MSM6J2Q1 PRJNA398089SRR5963947PRJNA398089SRR5936013human_gut
MSM6J2PS PRJNA398089SRR5963948PRJNA398089SRR5936231human_gut
\n" + ], + "text/latex": [ + "A tibble: 761 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t CSM67UC6 & PRJNA398089 & SRR5949109 & PRJNA398089 & SRR5936217 & human\\_gut\\\\\n", + "\t CSM67UDY & PRJNA398089 & SRR5949110 & PRJNA398089 & SRR5936216 & human\\_gut\\\\\n", + "\t HSM67VI9 & PRJNA398089 & SRR5949111 & PRJNA398089 & SRR5936212 & human\\_gut\\\\\n", + "\t HSM6XRTQ & PRJNA398089 & SRR5949112 & PRJNA398089 & SRR5936211 & human\\_gut\\\\\n", + "\t CSM79HHM & PRJNA398089 & SRR5949113 & PRJNA398089 & SRR5946811 & human\\_gut\\\\\n", + "\t CSM79HIR & PRJNA398089 & SRR5949114 & PRJNA398089 & SRR5936215 & human\\_gut\\\\\n", + "\t CSM79HJO & PRJNA398089 & SRR5949115 & PRJNA398089 & SRR5947089 & human\\_gut\\\\\n", + "\t HSM67VEI & PRJNA398089 & SRR5949116 & PRJNA398089 & SRR5936210 & human\\_gut\\\\\n", + "\t MSM79H9K & PRJNA398089 & SRR5949117 & PRJNA398089 & SRR5935975 & human\\_gut\\\\\n", + "\t MSM79HAH & PRJNA398089 & SRR5949118 & PRJNA398089 & SRR5935976 & human\\_gut\\\\\n", + "\t HSM7J4L5 & PRJNA398089 & SRR5949119 & PRJNA398089 & SRR5935953 & human\\_gut\\\\\n", + "\t MSM79H7E & PRJNA398089 & SRR5949120 & PRJNA398089 & SRR5935950 & human\\_gut\\\\\n", + "\t PSM7J17Z & PRJNA398089 & SRR5949121 & PRJNA398089 & SRR5935951 & human\\_gut\\\\\n", + "\t PSM7J18G & PRJNA398089 & SRR5949122 & PRJNA398089 & SRR5935952 & human\\_gut\\\\\n", + "\t MSM79HDI & PRJNA398089 & SRR5949123 & PRJNA398089 & SRR5935954 & human\\_gut\\\\\n", + "\t HSM7J4JH & PRJNA398089 & SRR5949124 & PRJNA398089 & SRR5935955 & human\\_gut\\\\\n", + "\t HSM7J4HU & PRJNA398089 & SRR5949125 & PRJNA398089 & SRR5935956 & human\\_gut\\\\\n", + "\t PSM7J1CI & PRJNA398089 & SRR5949126 & PRJNA398089 & SRR5935957 & human\\_gut\\\\\n", + "\t HSM7CZ36 & PRJNA398089 & SRR5949127 & PRJNA398089 & SRR5936016 & human\\_gut\\\\\n", + "\t CSM7KOJW & PRJNA398089 & SRR5949128 & PRJNA398089 & SRR5947102 & human\\_gut\\\\\n", + "\t MSM79HDM & PRJNA398089 & SRR5949129 & PRJNA398089 & SRR5936018 & human\\_gut\\\\\n", + "\t PSM6XBTP & PRJNA398089 & SRR5949130 & PRJNA398089 & SRR5936066 & human\\_gut\\\\\n", + "\t MSM79HF9 & PRJNA398089 & SRR5949131 & PRJNA398089 & SRR5936064 & human\\_gut\\\\\n", + "\t CSM79HQF & PRJNA398089 & SRR5949132 & PRJNA398089 & SRR5936119 & human\\_gut\\\\\n", + "\t MSM79H9A & PRJNA398089 & SRR5949133 & PRJNA398089 & SRR5936118 & human\\_gut\\\\\n", + "\t HSM7CZ3E & PRJNA398089 & SRR5949134 & PRJNA398089 & SRR5936121 & human\\_gut\\\\\n", + "\t CSM79HP4 & PRJNA398089 & SRR5949135 & PRJNA398089 & SRR5946645 & human\\_gut\\\\\n", + "\t HSM7CYY7 & PRJNA398089 & SRR5949136 & PRJNA398089 & SRR5936117 & human\\_gut\\\\\n", + "\t CSM79HLC & PRJNA398089 & SRR5949137 & PRJNA398089 & SRR5936176 & human\\_gut\\\\\n", + "\t MSM79H5K & PRJNA398089 & SRR5949138 & PRJNA398089 & SRR5936132 & human\\_gut\\\\\n", + "\t ⋮ & ⋮ & ⋮ & ⋮ & ⋮ & ⋮\\\\\n", + "\t PSM6XBSM & PRJNA398089 & SRR5963918 & PRJNA398089 & SRR5950713 & human\\_gut\\\\\n", + "\t MSM79HDQ\\_TR & PRJNA398089 & SRR5963920 & PRJNA398089 & SRR5962901 & human\\_gut\\\\\n", + "\t MSM79HDG\\_TR & PRJNA398089 & SRR5963921 & PRJNA398089 & SRR5962900 & human\\_gut\\\\\n", + "\t MSM9VZEK\\_TR & PRJNA398089 & SRR5963922 & PRJNA398089 & SRR5962903 & human\\_gut\\\\\n", + "\t MSM9VZMA\\_TR & PRJNA398089 & SRR5963923 & PRJNA398089 & SRR5962902 & human\\_gut\\\\\n", + "\t PSMA265J\\_TR & PRJNA398089 & SRR5963924 & PRJNA398089 & SRR5962904 & human\\_gut\\\\\n", + "\t MSMA26AZ\\_TR & PRJNA398089 & SRR5963925 & PRJNA398089 & SRR5962908 & human\\_gut\\\\\n", + "\t HSMA33LH\\_TR & PRJNA398089 & SRR5963926 & PRJNA398089 & SRR5962907 & human\\_gut\\\\\n", + "\t MSM9VZNH\\_TR & PRJNA398089 & SRR5963927 & PRJNA398089 & SRR5962906 & human\\_gut\\\\\n", + "\t HSMA33RX\\_TR & PRJNA398089 & SRR5963928 & PRJNA398089 & SRR5962885 & human\\_gut\\\\\n", + "\t PSM6XBQY\\_TR & PRJNA398089 & SRR5963929 & PRJNA398089 & SRR5962895 & human\\_gut\\\\\n", + "\t CSM79HLA\\_TR & PRJNA398089 & SRR5963930 & PRJNA398089 & SRR5962894 & human\\_gut\\\\\n", + "\t HSM6XRVC\\_TR & PRJNA398089 & SRR5963931 & PRJNA398089 & SRR5962893 & human\\_gut\\\\\n", + "\t HSM5MD6A\\_TR & PRJNA398089 & SRR5963932 & PRJNA398089 & SRR5962891 & human\\_gut\\\\\n", + "\t PSM6XBRK\\_TR & PRJNA398089 & SRR5963933 & PRJNA398089 & SRR5962890 & human\\_gut\\\\\n", + "\t CSM67UEW\\_TR & PRJNA398089 & SRR5963934 & PRJNA398089 & SRR5962889 & human\\_gut\\\\\n", + "\t HSM67VEM\\_TR & PRJNA398089 & SRR5963935 & PRJNA398089 & SRR5962892 & human\\_gut\\\\\n", + "\t CSM67UDR\\_TR & PRJNA398089 & SRR5963936 & PRJNA398089 & SRR5962888 & human\\_gut\\\\\n", + "\t PSM6XBSU\\_TR & PRJNA398089 & SRR5963937 & PRJNA398089 & SRR5962897 & human\\_gut\\\\\n", + "\t MSM79HF9\\_TR & PRJNA398089 & SRR5963938 & PRJNA398089 & SRR5962898 & human\\_gut\\\\\n", + "\t PSM6XBRK & PRJNA398089 & SRR5963939 & PRJNA398089 & SRR5935960 & human\\_gut\\\\\n", + "\t PSM6XBQY & PRJNA398089 & SRR5963940 & PRJNA398089 & SRR5935809 & human\\_gut\\\\\n", + "\t MSMA26EP & PRJNA398089 & SRR5963941 & PRJNA398089 & SRR5946720 & human\\_gut\\\\\n", + "\t MSM9VZOW & PRJNA398089 & SRR5963942 & PRJNA398089 & SRR5946733 & human\\_gut\\\\\n", + "\t PSM6XBQU & PRJNA398089 & SRR5963943 & PRJNA398089 & SRR5936007 & human\\_gut\\\\\n", + "\t MSMAPC5Z & PRJNA398089 & SRR5963944 & PRJNA398089 & SRR5946832 & human\\_gut\\\\\n", + "\t MSM6J2M3 & PRJNA398089 & SRR5963945 & PRJNA398089 & SRR5935963 & human\\_gut\\\\\n", + "\t MSM6J2K6 & PRJNA398089 & SRR5963946 & PRJNA398089 & SRR5935938 & human\\_gut\\\\\n", + "\t MSM6J2Q1 & PRJNA398089 & SRR5963947 & PRJNA398089 & SRR5936013 & human\\_gut\\\\\n", + "\t MSM6J2PS & PRJNA398089 & SRR5963948 & PRJNA398089 & SRR5936231 & human\\_gut\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 761 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| CSM67UC6 | PRJNA398089 | SRR5949109 | PRJNA398089 | SRR5936217 | human_gut |\n", + "| CSM67UDY | PRJNA398089 | SRR5949110 | PRJNA398089 | SRR5936216 | human_gut |\n", + "| HSM67VI9 | PRJNA398089 | SRR5949111 | PRJNA398089 | SRR5936212 | human_gut |\n", + "| HSM6XRTQ | PRJNA398089 | SRR5949112 | PRJNA398089 | SRR5936211 | human_gut |\n", + "| CSM79HHM | PRJNA398089 | SRR5949113 | PRJNA398089 | SRR5946811 | human_gut |\n", + "| CSM79HIR | PRJNA398089 | SRR5949114 | PRJNA398089 | SRR5936215 | human_gut |\n", + "| CSM79HJO | PRJNA398089 | SRR5949115 | PRJNA398089 | SRR5947089 | human_gut |\n", + "| HSM67VEI | PRJNA398089 | SRR5949116 | PRJNA398089 | SRR5936210 | human_gut |\n", + "| MSM79H9K | PRJNA398089 | SRR5949117 | PRJNA398089 | SRR5935975 | human_gut |\n", + "| MSM79HAH | PRJNA398089 | SRR5949118 | PRJNA398089 | SRR5935976 | human_gut |\n", + "| HSM7J4L5 | PRJNA398089 | SRR5949119 | PRJNA398089 | SRR5935953 | human_gut |\n", + "| MSM79H7E | PRJNA398089 | SRR5949120 | PRJNA398089 | SRR5935950 | human_gut |\n", + "| PSM7J17Z | PRJNA398089 | SRR5949121 | PRJNA398089 | SRR5935951 | human_gut |\n", + "| PSM7J18G | PRJNA398089 | SRR5949122 | PRJNA398089 | SRR5935952 | human_gut |\n", + "| MSM79HDI | PRJNA398089 | SRR5949123 | PRJNA398089 | SRR5935954 | human_gut |\n", + "| HSM7J4JH | PRJNA398089 | SRR5949124 | PRJNA398089 | SRR5935955 | human_gut |\n", + "| HSM7J4HU | PRJNA398089 | SRR5949125 | PRJNA398089 | SRR5935956 | human_gut |\n", + "| PSM7J1CI | PRJNA398089 | SRR5949126 | PRJNA398089 | SRR5935957 | human_gut |\n", + "| HSM7CZ36 | PRJNA398089 | SRR5949127 | PRJNA398089 | SRR5936016 | human_gut |\n", + "| CSM7KOJW | PRJNA398089 | SRR5949128 | PRJNA398089 | SRR5947102 | human_gut |\n", + "| MSM79HDM | PRJNA398089 | SRR5949129 | PRJNA398089 | SRR5936018 | human_gut |\n", + "| PSM6XBTP | PRJNA398089 | SRR5949130 | PRJNA398089 | SRR5936066 | human_gut |\n", + "| MSM79HF9 | PRJNA398089 | SRR5949131 | PRJNA398089 | SRR5936064 | human_gut |\n", + "| CSM79HQF | PRJNA398089 | SRR5949132 | PRJNA398089 | SRR5936119 | human_gut |\n", + "| MSM79H9A | PRJNA398089 | SRR5949133 | PRJNA398089 | SRR5936118 | human_gut |\n", + "| HSM7CZ3E | PRJNA398089 | SRR5949134 | PRJNA398089 | SRR5936121 | human_gut |\n", + "| CSM79HP4 | PRJNA398089 | SRR5949135 | PRJNA398089 | SRR5946645 | human_gut |\n", + "| HSM7CYY7 | PRJNA398089 | SRR5949136 | PRJNA398089 | SRR5936117 | human_gut |\n", + "| CSM79HLC | PRJNA398089 | SRR5949137 | PRJNA398089 | SRR5936176 | human_gut |\n", + "| MSM79H5K | PRJNA398089 | SRR5949138 | PRJNA398089 | SRR5936132 | human_gut |\n", + "| ⋮ | ⋮ | ⋮ | ⋮ | ⋮ | ⋮ |\n", + "| PSM6XBSM | PRJNA398089 | SRR5963918 | PRJNA398089 | SRR5950713 | human_gut |\n", + "| MSM79HDQ_TR | PRJNA398089 | SRR5963920 | PRJNA398089 | SRR5962901 | human_gut |\n", + "| MSM79HDG_TR | PRJNA398089 | SRR5963921 | PRJNA398089 | SRR5962900 | human_gut |\n", + "| MSM9VZEK_TR | PRJNA398089 | SRR5963922 | PRJNA398089 | SRR5962903 | human_gut |\n", + "| MSM9VZMA_TR | PRJNA398089 | SRR5963923 | PRJNA398089 | SRR5962902 | human_gut |\n", + "| PSMA265J_TR | PRJNA398089 | SRR5963924 | PRJNA398089 | SRR5962904 | human_gut |\n", + "| MSMA26AZ_TR | PRJNA398089 | SRR5963925 | PRJNA398089 | SRR5962908 | human_gut |\n", + "| HSMA33LH_TR | PRJNA398089 | SRR5963926 | PRJNA398089 | SRR5962907 | human_gut |\n", + "| MSM9VZNH_TR | PRJNA398089 | SRR5963927 | PRJNA398089 | SRR5962906 | human_gut |\n", + "| HSMA33RX_TR | PRJNA398089 | SRR5963928 | PRJNA398089 | SRR5962885 | human_gut |\n", + "| PSM6XBQY_TR | PRJNA398089 | SRR5963929 | PRJNA398089 | SRR5962895 | human_gut |\n", + "| CSM79HLA_TR | PRJNA398089 | SRR5963930 | PRJNA398089 | SRR5962894 | human_gut |\n", + "| HSM6XRVC_TR | PRJNA398089 | SRR5963931 | PRJNA398089 | SRR5962893 | human_gut |\n", + "| HSM5MD6A_TR | PRJNA398089 | SRR5963932 | PRJNA398089 | SRR5962891 | human_gut |\n", + "| PSM6XBRK_TR | PRJNA398089 | SRR5963933 | PRJNA398089 | SRR5962890 | human_gut |\n", + "| CSM67UEW_TR | PRJNA398089 | SRR5963934 | PRJNA398089 | SRR5962889 | human_gut |\n", + "| HSM67VEM_TR | PRJNA398089 | SRR5963935 | PRJNA398089 | SRR5962892 | human_gut |\n", + "| CSM67UDR_TR | PRJNA398089 | SRR5963936 | PRJNA398089 | SRR5962888 | human_gut |\n", + "| PSM6XBSU_TR | PRJNA398089 | SRR5963937 | PRJNA398089 | SRR5962897 | human_gut |\n", + "| MSM79HF9_TR | PRJNA398089 | SRR5963938 | PRJNA398089 | SRR5962898 | human_gut |\n", + "| PSM6XBRK | PRJNA398089 | SRR5963939 | PRJNA398089 | SRR5935960 | human_gut |\n", + "| PSM6XBQY | PRJNA398089 | SRR5963940 | PRJNA398089 | SRR5935809 | human_gut |\n", + "| MSMA26EP | PRJNA398089 | SRR5963941 | PRJNA398089 | SRR5946720 | human_gut |\n", + "| MSM9VZOW | PRJNA398089 | SRR5963942 | PRJNA398089 | SRR5946733 | human_gut |\n", + "| PSM6XBQU | PRJNA398089 | SRR5963943 | PRJNA398089 | SRR5936007 | human_gut |\n", + "| MSMAPC5Z | PRJNA398089 | SRR5963944 | PRJNA398089 | SRR5946832 | human_gut |\n", + "| MSM6J2M3 | PRJNA398089 | SRR5963945 | PRJNA398089 | SRR5935963 | human_gut |\n", + "| MSM6J2K6 | PRJNA398089 | SRR5963946 | PRJNA398089 | SRR5935938 | human_gut |\n", + "| MSM6J2Q1 | PRJNA398089 | SRR5963947 | PRJNA398089 | SRR5936013 | human_gut |\n", + "| MSM6J2PS | PRJNA398089 | SRR5963948 | PRJNA398089 | SRR5936231 | human_gut |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 CSM67UC6 PRJNA398089 SRR5949109 PRJNA398089 \n", + "2 CSM67UDY PRJNA398089 SRR5949110 PRJNA398089 \n", + "3 HSM67VI9 PRJNA398089 SRR5949111 PRJNA398089 \n", + "4 HSM6XRTQ PRJNA398089 SRR5949112 PRJNA398089 \n", + "5 CSM79HHM PRJNA398089 SRR5949113 PRJNA398089 \n", + "6 CSM79HIR PRJNA398089 SRR5949114 PRJNA398089 \n", + "7 CSM79HJO PRJNA398089 SRR5949115 PRJNA398089 \n", + "8 HSM67VEI PRJNA398089 SRR5949116 PRJNA398089 \n", + "9 MSM79H9K PRJNA398089 SRR5949117 PRJNA398089 \n", + "10 MSM79HAH PRJNA398089 SRR5949118 PRJNA398089 \n", + "11 HSM7J4L5 PRJNA398089 SRR5949119 PRJNA398089 \n", + "12 MSM79H7E PRJNA398089 SRR5949120 PRJNA398089 \n", + "13 PSM7J17Z PRJNA398089 SRR5949121 PRJNA398089 \n", + "14 PSM7J18G PRJNA398089 SRR5949122 PRJNA398089 \n", + "15 MSM79HDI PRJNA398089 SRR5949123 PRJNA398089 \n", + "16 HSM7J4JH PRJNA398089 SRR5949124 PRJNA398089 \n", + "17 HSM7J4HU PRJNA398089 SRR5949125 PRJNA398089 \n", + "18 PSM7J1CI PRJNA398089 SRR5949126 PRJNA398089 \n", + "19 HSM7CZ36 PRJNA398089 SRR5949127 PRJNA398089 \n", + "20 CSM7KOJW PRJNA398089 SRR5949128 PRJNA398089 \n", + "21 MSM79HDM PRJNA398089 SRR5949129 PRJNA398089 \n", + "22 PSM6XBTP PRJNA398089 SRR5949130 PRJNA398089 \n", + "23 MSM79HF9 PRJNA398089 SRR5949131 PRJNA398089 \n", + "24 CSM79HQF PRJNA398089 SRR5949132 PRJNA398089 \n", + "25 MSM79H9A PRJNA398089 SRR5949133 PRJNA398089 \n", + "26 HSM7CZ3E PRJNA398089 SRR5949134 PRJNA398089 \n", + "27 CSM79HP4 PRJNA398089 SRR5949135 PRJNA398089 \n", + "28 HSM7CYY7 PRJNA398089 SRR5949136 PRJNA398089 \n", + "29 CSM79HLC PRJNA398089 SRR5949137 PRJNA398089 \n", + "30 MSM79H5K PRJNA398089 SRR5949138 PRJNA398089 \n", + "⋮ ⋮ ⋮ ⋮ ⋮ \n", + "732 PSM6XBSM PRJNA398089 SRR5963918 PRJNA398089 \n", + "733 MSM79HDQ_TR PRJNA398089 SRR5963920 PRJNA398089 \n", + "734 MSM79HDG_TR PRJNA398089 SRR5963921 PRJNA398089 \n", + "735 MSM9VZEK_TR PRJNA398089 SRR5963922 PRJNA398089 \n", + "736 MSM9VZMA_TR PRJNA398089 SRR5963923 PRJNA398089 \n", + "737 PSMA265J_TR PRJNA398089 SRR5963924 PRJNA398089 \n", + "738 MSMA26AZ_TR PRJNA398089 SRR5963925 PRJNA398089 \n", + "739 HSMA33LH_TR PRJNA398089 SRR5963926 PRJNA398089 \n", + "740 MSM9VZNH_TR PRJNA398089 SRR5963927 PRJNA398089 \n", + "741 HSMA33RX_TR PRJNA398089 SRR5963928 PRJNA398089 \n", + "742 PSM6XBQY_TR PRJNA398089 SRR5963929 PRJNA398089 \n", + "743 CSM79HLA_TR PRJNA398089 SRR5963930 PRJNA398089 \n", + "744 HSM6XRVC_TR PRJNA398089 SRR5963931 PRJNA398089 \n", + "745 HSM5MD6A_TR PRJNA398089 SRR5963932 PRJNA398089 \n", + "746 PSM6XBRK_TR PRJNA398089 SRR5963933 PRJNA398089 \n", + "747 CSM67UEW_TR PRJNA398089 SRR5963934 PRJNA398089 \n", + "748 HSM67VEM_TR PRJNA398089 SRR5963935 PRJNA398089 \n", + "749 CSM67UDR_TR PRJNA398089 SRR5963936 PRJNA398089 \n", + "750 PSM6XBSU_TR PRJNA398089 SRR5963937 PRJNA398089 \n", + "751 MSM79HF9_TR PRJNA398089 SRR5963938 PRJNA398089 \n", + "752 PSM6XBRK PRJNA398089 SRR5963939 PRJNA398089 \n", + "753 PSM6XBQY PRJNA398089 SRR5963940 PRJNA398089 \n", + "754 MSMA26EP PRJNA398089 SRR5963941 PRJNA398089 \n", + "755 MSM9VZOW PRJNA398089 SRR5963942 PRJNA398089 \n", + "756 PSM6XBQU PRJNA398089 SRR5963943 PRJNA398089 \n", + "757 MSMAPC5Z PRJNA398089 SRR5963944 PRJNA398089 \n", + "758 MSM6J2M3 PRJNA398089 SRR5963945 PRJNA398089 \n", + "759 MSM6J2K6 PRJNA398089 SRR5963946 PRJNA398089 \n", + "760 MSM6J2Q1 PRJNA398089 SRR5963947 PRJNA398089 \n", + "761 MSM6J2PS PRJNA398089 SRR5963948 PRJNA398089 \n", + " mgx_run_accession sample_type\n", + "1 SRR5936217 human_gut \n", + "2 SRR5936216 human_gut \n", + "3 SRR5936212 human_gut \n", + "4 SRR5936211 human_gut \n", + "5 SRR5946811 human_gut \n", + "6 SRR5936215 human_gut \n", + "7 SRR5947089 human_gut \n", + "8 SRR5936210 human_gut \n", + "9 SRR5935975 human_gut \n", + "10 SRR5935976 human_gut \n", + "11 SRR5935953 human_gut \n", + "12 SRR5935950 human_gut \n", + "13 SRR5935951 human_gut \n", + "14 SRR5935952 human_gut \n", + "15 SRR5935954 human_gut \n", + "16 SRR5935955 human_gut \n", + "17 SRR5935956 human_gut \n", + "18 SRR5935957 human_gut \n", + "19 SRR5936016 human_gut \n", + "20 SRR5947102 human_gut \n", + "21 SRR5936018 human_gut \n", + "22 SRR5936066 human_gut \n", + "23 SRR5936064 human_gut \n", + "24 SRR5936119 human_gut \n", + "25 SRR5936118 human_gut \n", + "26 SRR5936121 human_gut \n", + "27 SRR5946645 human_gut \n", + "28 SRR5936117 human_gut \n", + "29 SRR5936176 human_gut \n", + "30 SRR5936132 human_gut \n", + "⋮ ⋮ ⋮ \n", + "732 SRR5950713 human_gut \n", + "733 SRR5962901 human_gut \n", + "734 SRR5962900 human_gut \n", + "735 SRR5962903 human_gut \n", + "736 SRR5962902 human_gut \n", + "737 SRR5962904 human_gut \n", + "738 SRR5962908 human_gut \n", + "739 SRR5962907 human_gut \n", + "740 SRR5962906 human_gut \n", + "741 SRR5962885 human_gut \n", + "742 SRR5962895 human_gut \n", + "743 SRR5962894 human_gut \n", + "744 SRR5962893 human_gut \n", + "745 SRR5962891 human_gut \n", + "746 SRR5962890 human_gut \n", + "747 SRR5962889 human_gut \n", + "748 SRR5962892 human_gut \n", + "749 SRR5962888 human_gut \n", + "750 SRR5962897 human_gut \n", + "751 SRR5962898 human_gut \n", + "752 SRR5935960 human_gut \n", + "753 SRR5935809 human_gut \n", + "754 SRR5946720 human_gut \n", + "755 SRR5946733 human_gut \n", + "756 SRR5936007 human_gut \n", + "757 SRR5946832 human_gut \n", + "758 SRR5935963 human_gut \n", + "759 SRR5935938 human_gut \n", + "760 SRR5936013 human_gut \n", + "761 SRR5936231 human_gut " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna398089_mtx <- prjna398089 %>%\n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " filter(library_strategy == \"RNA-Seq\") %>%\n", + " mutate(sample_name = gsub(\"_MTX\", \"\", experiment_alias)) %>%\n", + " select(sample_name, \n", + " mtx_study_accession = study_accession, \n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna398089_mgx <- prjna398089 %>%\n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " filter(library_strategy == \"WGS\") %>%\n", + " mutate(sample_name = gsub(\"_MGX\", \"\", experiment_alias)) %>%\n", + " select(sample_name, \n", + " mgx_study_accession = study_accession, \n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna398089_mtx_vs_mgx <- inner_join(prjna398089_mtx, prjna398089_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"human_gut\")\n", + "\n", + "prjna398089_mtx_vs_mgx " + ] + }, + { + "cell_type": "markdown", + "id": "f12fe413", + "metadata": {}, + "source": [ + "## PRJNA632343" + ] + }, + { + "cell_type": "code", + "execution_count": 61, + "id": "ce6d4776", + "metadata": {}, + "outputs": [], + "source": [ + "prjna632343 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA632343&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 71, + "id": "acd04061", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 4 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
Amar_Carlsberg-38I-DV129-2-N2 PRJNA632343SRR11781625PRJNA632343SRR11781636snail_gut
Amar_Carlsberg-38I-DV131-6-Intestine PRJNA632343SRR11781640PRJNA632343SRR11781639snail_gut
Amar_Carlsberg-38I-DV129-15-IntestinePRJNA632343SRR11781643PRJNA632343SRR11781642snail_gut
Amar_Carlsberg-38I-DV129-5-Intestine PRJNA632343SRR11781646PRJNA632343SRR11781645snail_gut
\n" + ], + "text/latex": [ + "A tibble: 4 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t Amar\\_Carlsberg-38I-DV129-2-N2 & PRJNA632343 & SRR11781625 & PRJNA632343 & SRR11781636 & snail\\_gut\\\\\n", + "\t Amar\\_Carlsberg-38I-DV131-6-Intestine & PRJNA632343 & SRR11781640 & PRJNA632343 & SRR11781639 & snail\\_gut\\\\\n", + "\t Amar\\_Carlsberg-38I-DV129-15-Intestine & PRJNA632343 & SRR11781643 & PRJNA632343 & SRR11781642 & snail\\_gut\\\\\n", + "\t Amar\\_Carlsberg-38I-DV129-5-Intestine & PRJNA632343 & SRR11781646 & PRJNA632343 & SRR11781645 & snail\\_gut\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 4 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| Amar_Carlsberg-38I-DV129-2-N2 | PRJNA632343 | SRR11781625 | PRJNA632343 | SRR11781636 | snail_gut |\n", + "| Amar_Carlsberg-38I-DV131-6-Intestine | PRJNA632343 | SRR11781640 | PRJNA632343 | SRR11781639 | snail_gut |\n", + "| Amar_Carlsberg-38I-DV129-15-Intestine | PRJNA632343 | SRR11781643 | PRJNA632343 | SRR11781642 | snail_gut |\n", + "| Amar_Carlsberg-38I-DV129-5-Intestine | PRJNA632343 | SRR11781646 | PRJNA632343 | SRR11781645 | snail_gut |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession\n", + "1 Amar_Carlsberg-38I-DV129-2-N2 PRJNA632343 SRR11781625 \n", + "2 Amar_Carlsberg-38I-DV131-6-Intestine PRJNA632343 SRR11781640 \n", + "3 Amar_Carlsberg-38I-DV129-15-Intestine PRJNA632343 SRR11781643 \n", + "4 Amar_Carlsberg-38I-DV129-5-Intestine PRJNA632343 SRR11781646 \n", + " mgx_study_accession mgx_run_accession sample_type\n", + "1 PRJNA632343 SRR11781636 snail_gut \n", + "2 PRJNA632343 SRR11781639 snail_gut \n", + "3 PRJNA632343 SRR11781642 snail_gut \n", + "4 PRJNA632343 SRR11781645 snail_gut " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna632343_mtx <- prjna632343 %>%\n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " filter(library_strategy == \"RNA-Seq\") %>%\n", + " mutate(sample_name = gsub(\"-Illumina-metaRNA\", \"\", experiment_alias),\n", + " sample_name = gsub(\"-Illumina-meta-RNA\", \"\", sample_name)) %>%\n", + " select(sample_name, \n", + " mtx_study_accession = study_accession, \n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna632343_mgx <- prjna632343 %>%\n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " filter(library_strategy == \"WGS\") %>%\n", + " filter(instrument_platform == \"ILLUMINA\") %>%\n", + " mutate(sample_name = gsub(\"-Illumina-metaDNA\", \"\", experiment_alias),\n", + " sample_name = gsub(\"-Illumina-meta-DNA\", \"\", sample_name)) %>%\n", + " select(sample_name, \n", + " mgx_study_accession = study_accession, \n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna632343_mtx_vs_mgx <- inner_join(prjna632343_mtx, prjna632343_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"snail_gut\")\n", + "\n", + "prjna632343_mtx_vs_mgx " + ] + }, + { + "cell_type": "markdown", + "id": "941acc7a", + "metadata": {}, + "source": [ + "## PRJNA242360" + ] + }, + { + "cell_type": "code", + "execution_count": 63, + "id": "5ce910c4", + "metadata": {}, + "outputs": [], + "source": [ + "prjna242360 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA242360&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 64, + "id": "d231a938", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 4 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
BATS_256_C3_1B PRJNA242360SRR1230757PRJNA242360SRR1230754ocean
BATS_261_C2_B2 PRJNA242360SRR1230758PRJNA242360SRR1230755ocean
BATS_261_C8_F2 PRJNA242360SRR1230759PRJNA242360SRR1230756ocean
BATS_256_C10_5APRJNA242360SRR1238005PRJNA242360SRR1230729ocean
\n" + ], + "text/latex": [ + "A tibble: 4 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t BATS\\_256\\_C3\\_1B & PRJNA242360 & SRR1230757 & PRJNA242360 & SRR1230754 & ocean\\\\\n", + "\t BATS\\_261\\_C2\\_B2 & PRJNA242360 & SRR1230758 & PRJNA242360 & SRR1230755 & ocean\\\\\n", + "\t BATS\\_261\\_C8\\_F2 & PRJNA242360 & SRR1230759 & PRJNA242360 & SRR1230756 & ocean\\\\\n", + "\t BATS\\_256\\_C10\\_5A & PRJNA242360 & SRR1238005 & PRJNA242360 & SRR1230729 & ocean\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 4 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| BATS_256_C3_1B | PRJNA242360 | SRR1230757 | PRJNA242360 | SRR1230754 | ocean |\n", + "| BATS_261_C2_B2 | PRJNA242360 | SRR1230758 | PRJNA242360 | SRR1230755 | ocean |\n", + "| BATS_261_C8_F2 | PRJNA242360 | SRR1230759 | PRJNA242360 | SRR1230756 | ocean |\n", + "| BATS_256_C10_5A | PRJNA242360 | SRR1238005 | PRJNA242360 | SRR1230729 | ocean |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 BATS_256_C3_1B PRJNA242360 SRR1230757 PRJNA242360 \n", + "2 BATS_261_C2_B2 PRJNA242360 SRR1230758 PRJNA242360 \n", + "3 BATS_261_C8_F2 PRJNA242360 SRR1230759 PRJNA242360 \n", + "4 BATS_256_C10_5A PRJNA242360 SRR1238005 PRJNA242360 \n", + " mgx_run_accession sample_type\n", + "1 SRR1230754 ocean \n", + "2 SRR1230755 ocean \n", + "3 SRR1230756 ocean \n", + "4 SRR1230729 ocean " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna242360_mtx <- prjna242360 %>%\n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " filter(instrument_platform == \"ILLUMINA\") %>%\n", + " mutate(sample_name = gsub(\"Illumina HiSeq 2500 sequencing; \", \"\", experiment_title),\n", + " sample_name = gsub(\"_M[GT]\", \"\", sample_name),\n", + " sample_name = gsub(\"_R1R2\", \"\", sample_name),\n", + " sample_name = gsub(\"C2_2\", \"C2_B2\", sample_name),\n", + " sample_name = gsub(\"C8_2\", \"C8_F2\", sample_name)) %>%\n", + " select(sample_name, \n", + " mtx_study_accession = study_accession, \n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna242360_mgx <- prjna242360 %>%\n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " filter(library_strategy == \"WGS\") %>%\n", + " filter(instrument_platform == \"ILLUMINA\") %>%\n", + " mutate(sample_name = gsub(\"Illumina HiSeq 2500 sequencing; \", \"\", experiment_title),\n", + " sample_name = gsub(\"_M[GT]\", \"\", sample_name),\n", + " sample_name = gsub(\"_R1R2\", \"\", sample_name),\n", + " sample_name = gsub(\"C2_2\", \"C2_B2\", sample_name),\n", + " sample_name = gsub(\"C8_2\", \"C8_F2\", sample_name)) %>%\n", + " select(sample_name, \n", + " mgx_study_accession = study_accession, \n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna242360_mtx_vs_mgx <- inner_join(prjna242360_mtx, prjna242360_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"ocean\")\n", + "\n", + "prjna242360_mtx_vs_mgx " + ] + }, + { + "cell_type": "markdown", + "id": "0f3958d4", + "metadata": {}, + "source": [ + "## PRJEB12234" + ] + }, + { + "cell_type": "code", + "execution_count": 65, + "id": "7b4cd065", + "metadata": {}, + "outputs": [], + "source": [ + "prjeb12234 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJEB12234&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 66, + "id": "a965057c", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 13 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
ES_08072017_CAT2012July PRJEB12234ERR2088997PRJEB12234ERR2094669ocean
ES_08072017_CAT2012October PRJEB12234ERR2088998PRJEB12234ERR2094670ocean
ES_08072017_CAT2013January PRJEB12234ERR2088999PRJEB12234ERR2094671ocean
ES_08072017_CAT2013April PRJEB12234ERR2089000PRJEB12234ERR2094672ocean
ES_08072017_POLA2012July PRJEB12234ERR2089001PRJEB12234ERR2094673ocean
ES_08072017_POLA2012OctoberPRJEB12234ERR2089002PRJEB12234ERR2094674ocean
ES_08072017_POLA2013JanuaryPRJEB12234ERR2089003PRJEB12234ERR2094675ocean
ES_08072017_POLA2013April PRJEB12234ERR2089004PRJEB12234ERR2094676ocean
ES_08072017_SPOT2012July PRJEB12234ERR2089005PRJEB12234ERR2094677ocean
ES_08072017_SPOT2012OctoberPRJEB12234ERR2089006PRJEB12234ERR2094678ocean
ES_08072017_SPOT2013JanuaryPRJEB12234ERR2089007PRJEB12234ERR2094679ocean
ES_08072017_SPOT2013April PRJEB12234ERR2089008PRJEB12234ERR2094680ocean
ES_08072017_neg_reads PRJEB12234ERR2089009PRJEB12234ERR2097133ocean
\n" + ], + "text/latex": [ + "A tibble: 13 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t ES\\_08072017\\_CAT2012July & PRJEB12234 & ERR2088997 & PRJEB12234 & ERR2094669 & ocean\\\\\n", + "\t ES\\_08072017\\_CAT2012October & PRJEB12234 & ERR2088998 & PRJEB12234 & ERR2094670 & ocean\\\\\n", + "\t ES\\_08072017\\_CAT2013January & PRJEB12234 & ERR2088999 & PRJEB12234 & ERR2094671 & ocean\\\\\n", + "\t ES\\_08072017\\_CAT2013April & PRJEB12234 & ERR2089000 & PRJEB12234 & ERR2094672 & ocean\\\\\n", + "\t ES\\_08072017\\_POLA2012July & PRJEB12234 & ERR2089001 & PRJEB12234 & ERR2094673 & ocean\\\\\n", + "\t ES\\_08072017\\_POLA2012October & PRJEB12234 & ERR2089002 & PRJEB12234 & ERR2094674 & ocean\\\\\n", + "\t ES\\_08072017\\_POLA2013January & PRJEB12234 & ERR2089003 & PRJEB12234 & ERR2094675 & ocean\\\\\n", + "\t ES\\_08072017\\_POLA2013April & PRJEB12234 & ERR2089004 & PRJEB12234 & ERR2094676 & ocean\\\\\n", + "\t ES\\_08072017\\_SPOT2012July & PRJEB12234 & ERR2089005 & PRJEB12234 & ERR2094677 & ocean\\\\\n", + "\t ES\\_08072017\\_SPOT2012October & PRJEB12234 & ERR2089006 & PRJEB12234 & ERR2094678 & ocean\\\\\n", + "\t ES\\_08072017\\_SPOT2013January & PRJEB12234 & ERR2089007 & PRJEB12234 & ERR2094679 & ocean\\\\\n", + "\t ES\\_08072017\\_SPOT2013April & PRJEB12234 & ERR2089008 & PRJEB12234 & ERR2094680 & ocean\\\\\n", + "\t ES\\_08072017\\_neg\\_reads & PRJEB12234 & ERR2089009 & PRJEB12234 & ERR2097133 & ocean\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 13 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| ES_08072017_CAT2012July | PRJEB12234 | ERR2088997 | PRJEB12234 | ERR2094669 | ocean |\n", + "| ES_08072017_CAT2012October | PRJEB12234 | ERR2088998 | PRJEB12234 | ERR2094670 | ocean |\n", + "| ES_08072017_CAT2013January | PRJEB12234 | ERR2088999 | PRJEB12234 | ERR2094671 | ocean |\n", + "| ES_08072017_CAT2013April | PRJEB12234 | ERR2089000 | PRJEB12234 | ERR2094672 | ocean |\n", + "| ES_08072017_POLA2012July | PRJEB12234 | ERR2089001 | PRJEB12234 | ERR2094673 | ocean |\n", + "| ES_08072017_POLA2012October | PRJEB12234 | ERR2089002 | PRJEB12234 | ERR2094674 | ocean |\n", + "| ES_08072017_POLA2013January | PRJEB12234 | ERR2089003 | PRJEB12234 | ERR2094675 | ocean |\n", + "| ES_08072017_POLA2013April | PRJEB12234 | ERR2089004 | PRJEB12234 | ERR2094676 | ocean |\n", + "| ES_08072017_SPOT2012July | PRJEB12234 | ERR2089005 | PRJEB12234 | ERR2094677 | ocean |\n", + "| ES_08072017_SPOT2012October | PRJEB12234 | ERR2089006 | PRJEB12234 | ERR2094678 | ocean |\n", + "| ES_08072017_SPOT2013January | PRJEB12234 | ERR2089007 | PRJEB12234 | ERR2094679 | ocean |\n", + "| ES_08072017_SPOT2013April | PRJEB12234 | ERR2089008 | PRJEB12234 | ERR2094680 | ocean |\n", + "| ES_08072017_neg_reads | PRJEB12234 | ERR2089009 | PRJEB12234 | ERR2097133 | ocean |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession\n", + "1 ES_08072017_CAT2012July PRJEB12234 ERR2088997 \n", + "2 ES_08072017_CAT2012October PRJEB12234 ERR2088998 \n", + "3 ES_08072017_CAT2013January PRJEB12234 ERR2088999 \n", + "4 ES_08072017_CAT2013April PRJEB12234 ERR2089000 \n", + "5 ES_08072017_POLA2012July PRJEB12234 ERR2089001 \n", + "6 ES_08072017_POLA2012October PRJEB12234 ERR2089002 \n", + "7 ES_08072017_POLA2013January PRJEB12234 ERR2089003 \n", + "8 ES_08072017_POLA2013April PRJEB12234 ERR2089004 \n", + "9 ES_08072017_SPOT2012July PRJEB12234 ERR2089005 \n", + "10 ES_08072017_SPOT2012October PRJEB12234 ERR2089006 \n", + "11 ES_08072017_SPOT2013January PRJEB12234 ERR2089007 \n", + "12 ES_08072017_SPOT2013April PRJEB12234 ERR2089008 \n", + "13 ES_08072017_neg_reads PRJEB12234 ERR2089009 \n", + " mgx_study_accession mgx_run_accession sample_type\n", + "1 PRJEB12234 ERR2094669 ocean \n", + "2 PRJEB12234 ERR2094670 ocean \n", + "3 PRJEB12234 ERR2094671 ocean \n", + "4 PRJEB12234 ERR2094672 ocean \n", + "5 PRJEB12234 ERR2094673 ocean \n", + "6 PRJEB12234 ERR2094674 ocean \n", + "7 PRJEB12234 ERR2094675 ocean \n", + "8 PRJEB12234 ERR2094676 ocean \n", + "9 PRJEB12234 ERR2094677 ocean \n", + "10 PRJEB12234 ERR2094678 ocean \n", + "11 PRJEB12234 ERR2094679 ocean \n", + "12 PRJEB12234 ERR2094680 ocean \n", + "13 PRJEB12234 ERR2097133 ocean " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjeb12234_mtx <- prjeb12234 %>%\n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " filter(library_strategy == \"WGS\") %>%\n", + " mutate(sample_name = gsub(\"_MT\", \"\", sample_alias)) %>%\n", + " select(sample_name, \n", + " mtx_study_accession = study_accession, \n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjeb12234_mgx <- prjeb12234 %>%\n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " filter(library_strategy == \"WGS\") %>%\n", + " mutate(sample_name = gsub(\"_MG\", \"\", sample_alias)) %>%\n", + " select(sample_name, \n", + " mgx_study_accession = study_accession, \n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjeb12234_mtx_vs_mgx <- inner_join(prjeb12234_mtx, prjeb12234_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"ocean\")\n", + "\n", + "prjeb12234_mtx_vs_mgx " + ] + }, + { + "cell_type": "markdown", + "id": "419d1e0e", + "metadata": {}, + "source": [ + "## PRJNA340003" + ] + }, + { + "cell_type": "code", + "execution_count": 67, + "id": "308ec27f", + "metadata": {}, + "outputs": [], + "source": [ + "prjna340003 <- read_tsv(\"https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA340003&result=read_run&fields=study_accession,secondary_study_accession,sample_accession,secondary_sample_accession,experiment_accession,run_accession,submission_accession,tax_id,scientific_name,instrument_platform,instrument_model,library_name,nominal_length,library_layout,library_strategy,library_source,library_selection,read_count,base_count,center_name,first_public,last_updated,experiment_title,study_title,study_alias,experiment_alias,run_alias,fastq_bytes,fastq_md5,fastq_ftp,fastq_aspera,fastq_galaxy,submitted_bytes,submitted_md5,submitted_ftp,submitted_aspera,submitted_galaxy,submitted_format,sra_bytes,sra_md5,sra_ftp,sra_aspera,sra_galaxy,cram_index_ftp,cram_index_aspera,cram_index_galaxy,sample_alias,broker_name,sample_title,nominal_sdev,first_created&format=tsv&download=true&limit=0\",\n", + " show_col_types = F)" + ] + }, + { + "cell_type": "code", + "execution_count": 68, + "id": "b9428e0a", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 6 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
E2APRJNA340003SRR4342131PRJNA340003SRR4342135ocean
E4 PRJNA340003SRR4342132PRJNA340003SRR4342136ocean
D1 PRJNA340003SRR4342137PRJNA340003SRR4342129ocean
D2 PRJNA340003SRR4342138PRJNA340003SRR4342130ocean
D3 PRJNA340003SRR4342139PRJNA340003SRR4342133ocean
E2 PRJNA340003SRR4342140PRJNA340003SRR4342134ocean
\n" + ], + "text/latex": [ + "A tibble: 6 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t E2A & PRJNA340003 & SRR4342131 & PRJNA340003 & SRR4342135 & ocean\\\\\n", + "\t E4 & PRJNA340003 & SRR4342132 & PRJNA340003 & SRR4342136 & ocean\\\\\n", + "\t D1 & PRJNA340003 & SRR4342137 & PRJNA340003 & SRR4342129 & ocean\\\\\n", + "\t D2 & PRJNA340003 & SRR4342138 & PRJNA340003 & SRR4342130 & ocean\\\\\n", + "\t D3 & PRJNA340003 & SRR4342139 & PRJNA340003 & SRR4342133 & ocean\\\\\n", + "\t E2 & PRJNA340003 & SRR4342140 & PRJNA340003 & SRR4342134 & ocean\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 6 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| E2A | PRJNA340003 | SRR4342131 | PRJNA340003 | SRR4342135 | ocean |\n", + "| E4 | PRJNA340003 | SRR4342132 | PRJNA340003 | SRR4342136 | ocean |\n", + "| D1 | PRJNA340003 | SRR4342137 | PRJNA340003 | SRR4342129 | ocean |\n", + "| D2 | PRJNA340003 | SRR4342138 | PRJNA340003 | SRR4342130 | ocean |\n", + "| D3 | PRJNA340003 | SRR4342139 | PRJNA340003 | SRR4342133 | ocean |\n", + "| E2 | PRJNA340003 | SRR4342140 | PRJNA340003 | SRR4342134 | ocean |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 E2A PRJNA340003 SRR4342131 PRJNA340003 \n", + "2 E4 PRJNA340003 SRR4342132 PRJNA340003 \n", + "3 D1 PRJNA340003 SRR4342137 PRJNA340003 \n", + "4 D2 PRJNA340003 SRR4342138 PRJNA340003 \n", + "5 D3 PRJNA340003 SRR4342139 PRJNA340003 \n", + "6 E2 PRJNA340003 SRR4342140 PRJNA340003 \n", + " mgx_run_accession sample_type\n", + "1 SRR4342135 ocean \n", + "2 SRR4342136 ocean \n", + "3 SRR4342129 ocean \n", + "4 SRR4342130 ocean \n", + "5 SRR4342133 ocean \n", + "6 SRR4342134 ocean " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "prjna340003_mtx <- prjna340003 %>%\n", + " filter(library_source == \"METATRANSCRIPTOMIC\") %>%\n", + " mutate(sample_name = gsub(\":RNA\", \"\", sample_alias)) %>%\n", + " select(sample_name, \n", + " mtx_study_accession = study_accession, \n", + " mtx_run_accession = run_accession)\n", + "\n", + "prjna340003_mgx <- prjna340003 %>%\n", + " filter(library_source == \"METAGENOMIC\") %>%\n", + " mutate(sample_name = gsub(\":DNA\", \"\", sample_alias)) %>%\n", + " select(sample_name, \n", + " mgx_study_accession = study_accession, \n", + " mgx_run_accession = run_accession)\n", + "\n", + "prjna340003_mtx_vs_mgx <- inner_join(prjna340003_mtx, prjna340003_mgx, by = \"sample_name\") %>%\n", + " mutate(sample_type = \"ocean\")\n", + "\n", + "prjna340003_mtx_vs_mgx " + ] + }, + { + "cell_type": "markdown", + "id": "fc5a0dc6", + "metadata": {}, + "source": [ + "# Combine everything together" + ] + }, + { + "cell_type": "code", + "execution_count": 72, + "id": "0aace26d", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 6 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
C_2_1 PRJNA406858SRR6032600PRJNA406858SRR6032602activated_sludge
B_2_1 PRJNA406858SRR6032604PRJNA406858SRR6032601activated_sludge
E_2_1 PRJNA406858SRR6032605PRJNA406858SRR6032603activated_sludge
rumen_microbiome_of_beef_cattle_101PRJNA448333SRR8416057PRJNA448333SRR8397906cattle_rumen
rumen_microbiome_of_beef_cattle_103PRJNA448333SRR8416058PRJNA448333SRR8404214cattle_rumen
rumen_microbiome_of_beef_cattle_104PRJNA448333SRR8416055PRJNA448333SRR8397905cattle_rumen
\n" + ], + "text/latex": [ + "A tibble: 6 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t C\\_2\\_1 & PRJNA406858 & SRR6032600 & PRJNA406858 & SRR6032602 & activated\\_sludge\\\\\n", + "\t B\\_2\\_1 & PRJNA406858 & SRR6032604 & PRJNA406858 & SRR6032601 & activated\\_sludge\\\\\n", + "\t E\\_2\\_1 & PRJNA406858 & SRR6032605 & PRJNA406858 & SRR6032603 & activated\\_sludge\\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_101 & PRJNA448333 & SRR8416057 & PRJNA448333 & SRR8397906 & cattle\\_rumen \\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_103 & PRJNA448333 & SRR8416058 & PRJNA448333 & SRR8404214 & cattle\\_rumen \\\\\n", + "\t rumen\\_microbiome\\_of\\_beef\\_cattle\\_104 & PRJNA448333 & SRR8416055 & PRJNA448333 & SRR8397905 & cattle\\_rumen \\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 6 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| C_2_1 | PRJNA406858 | SRR6032600 | PRJNA406858 | SRR6032602 | activated_sludge |\n", + "| B_2_1 | PRJNA406858 | SRR6032604 | PRJNA406858 | SRR6032601 | activated_sludge |\n", + "| E_2_1 | PRJNA406858 | SRR6032605 | PRJNA406858 | SRR6032603 | activated_sludge |\n", + "| rumen_microbiome_of_beef_cattle_101 | PRJNA448333 | SRR8416057 | PRJNA448333 | SRR8397906 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_103 | PRJNA448333 | SRR8416058 | PRJNA448333 | SRR8404214 | cattle_rumen |\n", + "| rumen_microbiome_of_beef_cattle_104 | PRJNA448333 | SRR8416055 | PRJNA448333 | SRR8397905 | cattle_rumen |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession\n", + "1 C_2_1 PRJNA406858 SRR6032600 \n", + "2 B_2_1 PRJNA406858 SRR6032604 \n", + "3 E_2_1 PRJNA406858 SRR6032605 \n", + "4 rumen_microbiome_of_beef_cattle_101 PRJNA448333 SRR8416057 \n", + "5 rumen_microbiome_of_beef_cattle_103 PRJNA448333 SRR8416058 \n", + "6 rumen_microbiome_of_beef_cattle_104 PRJNA448333 SRR8416055 \n", + " mgx_study_accession mgx_run_accession sample_type \n", + "1 PRJNA406858 SRR6032602 activated_sludge\n", + "2 PRJNA406858 SRR6032601 activated_sludge\n", + "3 PRJNA406858 SRR6032603 activated_sludge\n", + "4 PRJNA448333 SRR8397906 cattle_rumen \n", + "5 PRJNA448333 SRR8404214 cattle_rumen \n", + "6 PRJNA448333 SRR8397905 cattle_rumen " + ] + }, + "metadata": {}, + "output_type": "display_data" + }, + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 6 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
E2APRJNA340003SRR4342131PRJNA340003SRR4342135ocean
E4 PRJNA340003SRR4342132PRJNA340003SRR4342136ocean
D1 PRJNA340003SRR4342137PRJNA340003SRR4342129ocean
D2 PRJNA340003SRR4342138PRJNA340003SRR4342130ocean
D3 PRJNA340003SRR4342139PRJNA340003SRR4342133ocean
E2 PRJNA340003SRR4342140PRJNA340003SRR4342134ocean
\n" + ], + "text/latex": [ + "A tibble: 6 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\t E2A & PRJNA340003 & SRR4342131 & PRJNA340003 & SRR4342135 & ocean\\\\\n", + "\t E4 & PRJNA340003 & SRR4342132 & PRJNA340003 & SRR4342136 & ocean\\\\\n", + "\t D1 & PRJNA340003 & SRR4342137 & PRJNA340003 & SRR4342129 & ocean\\\\\n", + "\t D2 & PRJNA340003 & SRR4342138 & PRJNA340003 & SRR4342130 & ocean\\\\\n", + "\t D3 & PRJNA340003 & SRR4342139 & PRJNA340003 & SRR4342133 & ocean\\\\\n", + "\t E2 & PRJNA340003 & SRR4342140 & PRJNA340003 & SRR4342134 & ocean\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 6 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "| E2A | PRJNA340003 | SRR4342131 | PRJNA340003 | SRR4342135 | ocean |\n", + "| E4 | PRJNA340003 | SRR4342132 | PRJNA340003 | SRR4342136 | ocean |\n", + "| D1 | PRJNA340003 | SRR4342137 | PRJNA340003 | SRR4342129 | ocean |\n", + "| D2 | PRJNA340003 | SRR4342138 | PRJNA340003 | SRR4342130 | ocean |\n", + "| D3 | PRJNA340003 | SRR4342139 | PRJNA340003 | SRR4342133 | ocean |\n", + "| E2 | PRJNA340003 | SRR4342140 | PRJNA340003 | SRR4342134 | ocean |\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + "1 E2A PRJNA340003 SRR4342131 PRJNA340003 \n", + "2 E4 PRJNA340003 SRR4342132 PRJNA340003 \n", + "3 D1 PRJNA340003 SRR4342137 PRJNA340003 \n", + "4 D2 PRJNA340003 SRR4342138 PRJNA340003 \n", + "5 D3 PRJNA340003 SRR4342139 PRJNA340003 \n", + "6 E2 PRJNA340003 SRR4342140 PRJNA340003 \n", + " mgx_run_accession sample_type\n", + "1 SRR4342135 ocean \n", + "2 SRR4342136 ocean \n", + "3 SRR4342129 ocean \n", + "4 SRR4342130 ocean \n", + "5 SRR4342133 ocean \n", + "6 SRR4342134 ocean " + ] + }, + "metadata": {}, + "output_type": "display_data" + }, + { + "data": { + "text/html": [ + "1280" + ], + "text/latex": [ + "1280" + ], + "text/markdown": [ + "1280" + ], + "text/plain": [ + "[1] 1280" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "all_paired_mtx_mgx <- bind_rows(prjna406858_mtx_vs_mgx,\n", + " prjna448333_mtx_vs_mgx,\n", + " prjna344005_mtx_vs_mgx,\n", + " prjna453733_mtx_vs_mgx,\n", + " prjna237345_vs_prjna237344,\n", + " moran010B_mtx_vs_mgx,\n", + " prjna603240_mtx_vs_mgx,\n", + " prjna202380_mtx_vs_mgx,\n", + " prjna541981_mtx_vs_mgx,\n", + " prjna797778_mtx_vs_mgx,\n", + " prjna339914_mtx_vs_mgx,\n", + " prjeb33889_mtx_vs_mgx,\n", + " prjna698464_mtx_vs_mgx,\n", + " prjna396840_mtx_vs_mgx,\n", + " prjna492158_mtx_vs_mgx,\n", + " prjna278075_mtx_vs_mgx,\n", + " prjeb38017_mtx_vs_mgx,\n", + " prjna616041_mtx_vs_mgx,\n", + " prjna395125_vs_prjna393770,\n", + " prjeb12284_vs_prjeb12083,\n", + " prjeb32788_vs_prjeb32781,\n", + " prjna398089_mtx_vs_mgx,\n", + " prjna632343_mtx_vs_mgx,\n", + " prjna242360_mtx_vs_mgx,\n", + " prjeb12234_mtx_vs_mgx,\n", + " prjna340003_mtx_vs_mgx)\n", + "head(all_paired_mtx_mgx)\n", + "tail(all_paired_mtx_mgx)\n", + "nrow(all_paired_mtx_mgx)" + ] + }, + { + "cell_type": "code", + "execution_count": 73, + "id": "f6f62f8e", + "metadata": {}, + "outputs": [ + { + "data": { + "text/plain": [ + "\n", + " activated_sludge bioreactor cattle_rumen \n", + " 3 11 48 \n", + "cocoa_box_fermentation deadwood groundwater \n", + " 3 10 2 \n", + " human_gut human_oral human_skin \n", + " 791 30 17 \n", + " human_vagina lake mouse_cecum \n", + " 180 1 8 \n", + " mussel_gill ocean paddy_soil \n", + " 17 98 6 \n", + " river sheep_rumen snail_gut \n", + " 23 20 4 \n", + " wastewater \n", + " 8 " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "# look at the breakdown of sample types\n", + "table(all_paired_mtx_mgx$sample_type)" + ] + }, + { + "cell_type": "code", + "execution_count": 74, + "id": "8d49c7ee", + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "1280" + ], + "text/latex": [ + "1280" + ], + "text/markdown": [ + "1280" + ], + "text/plain": [ + "[1] 1280" + ] + }, + "metadata": {}, + "output_type": "display_data" + }, + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\n", + "
A tibble: 0 × 6
sample_namemtx_study_accessionmtx_run_accessionmgx_study_accessionmgx_run_accessionsample_type
<chr><chr><chr><chr><chr><chr>
\n" + ], + "text/latex": [ + "A tibble: 0 × 6\n", + "\\begin{tabular}{llllll}\n", + " sample\\_name & mtx\\_study\\_accession & mtx\\_run\\_accession & mgx\\_study\\_accession & mgx\\_run\\_accession & sample\\_type\\\\\n", + " & & & & & \\\\\n", + "\\hline\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 0 × 6\n", + "\n", + "| sample_name <chr> | mtx_study_accession <chr> | mtx_run_accession <chr> | mgx_study_accession <chr> | mgx_run_accession <chr> | sample_type <chr> |\n", + "|---|---|---|---|---|---|\n", + "\n" + ], + "text/plain": [ + " sample_name mtx_study_accession mtx_run_accession mgx_study_accession\n", + " mgx_run_accession sample_type" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "# check and make sure there are no duplicated sample names, \n", + "# either from faulty joins for from x-study shared sample identifiers\n", + "length(unique(all_paired_mtx_mgx$sample_name))\n", + "\n", + "tmp <- all_paired_mtx_mgx %>% \n", + " group_by(sample_name) %>%\n", + " tally() %>%\n", + " filter(n > 1)\n", + "\n", + "all_paired_mtx_mgx %>%\n", + " filter(sample_name %in% tmp$sample_name)" + ] + }, + { + "cell_type": "markdown", + "id": "cffc574e", + "metadata": {}, + "source": [ + "## Write out tsv with results" + ] + }, + { + "cell_type": "code", + "execution_count": 75, + "id": "63466e52", + "metadata": {}, + "outputs": [], + "source": [ + "write_tsv(all_paired_mtx_mgx, \"inputs/metadata-paired-mgx-mtx.tsv\")" + ] + }, + { + "cell_type": "markdown", + "id": "56293500", + "metadata": {}, + "source": [ + "## Session Info" + ] + }, + { + "cell_type": "code", + "execution_count": 76, + "id": "c886393d", + "metadata": {}, + "outputs": [ + { + "data": { + "text/plain": [ + "R version 4.1.3 (2022-03-10)\n", + "Platform: x86_64-apple-darwin13.4.0 (64-bit)\n", + "Running under: macOS Big Sur/Monterey 10.16\n", + "\n", + "Matrix products: default\n", + "BLAS/LAPACK: /Users/taylorreiter/miniconda3/envs/tidyjupyter/lib/libopenblasp-r0.3.21.dylib\n", + "\n", + "locale:\n", + "[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8\n", + "\n", + "attached base packages:\n", + "[1] stats graphics grDevices utils datasets methods base \n", + "\n", + "other attached packages:\n", + "[1] janitor_2.1.0 readr_2.1.2 dplyr_1.0.9 \n", + "\n", + "loaded via a namespace (and not attached):\n", + " [1] pillar_1.8.1 compiler_4.1.3 base64enc_0.1-3 tools_4.1.3 \n", + " [5] bit_4.0.4 digest_0.6.29 uuid_1.1-0 jsonlite_1.8.0 \n", + " [9] lubridate_1.8.0 evaluate_0.16 lifecycle_1.0.1 tibble_3.1.8 \n", + "[13] pkgconfig_2.0.3 rlang_1.0.4 IRdisplay_1.1 cli_3.3.0 \n", + "[17] DBI_1.1.3 curl_4.3.2 parallel_4.1.3 IRkernel_1.3 \n", + "[21] fastmap_1.1.0 stringr_1.4.0 repr_1.1.4 generics_0.1.3 \n", + "[25] vctrs_0.4.1 hms_1.1.2 bit64_4.0.5 tidyselect_1.1.2\n", + "[29] glue_1.6.2 snakecase_0.11.0 R6_2.5.1 fansi_1.0.3 \n", + "[33] vroom_1.5.7 pbdZMQ_0.3-7 purrr_0.3.4 tzdb_0.3.0 \n", + "[37] magrittr_2.0.3 htmltools_0.5.3 ellipsis_0.3.2 assertthat_0.2.1\n", + "[41] utf8_1.2.2 stringi_1.7.8 crayon_1.5.1 " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "sessionInfo()" + ] + } + ], + "metadata": { + "kernelspec": { + "display_name": "R", + "language": "R", + "name": "ir" + }, + "language_info": { + "codemirror_mode": "r", + "file_extension": ".r", + "mimetype": "text/x-r-source", + "name": "R", + "pygments_lexer": "r", + "version": "4.1.3" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +}