diff --git a/bbtools/Dockerfile b/bbtools/Dockerfile
new file mode 100644
index 0000000..d5a7182
--- /dev/null
+++ b/bbtools/Dockerfile
@@ -0,0 +1,19 @@
+# DOCKERFILE for BBtools
+FROM ubuntu:16.04
+MAINTAINER Pedro Cerqueira, pedro.cerqueira@medicina.ulisboa.pt
+
+#Update and install dependencies
+RUN apt-get update && apt-get -y install \
+    bash \
+	wget \
+    default-jdk
+
+WORKDIR /NGStools/
+
+#Download and untar BBtools
+RUN wget https://sourceforge.net/projects/bbmap/files/BBMap_38.44.tar.gz/download -O bbtools.tar.gz && tar -xvzf bbtools.tar.gz
+
+RUN rm bbtools.tar.gz
+
+#Add BBtools to environment variable
+ENV PATH="/NGStools/bbmap:$PATH"
diff --git a/seqtk/Dockerfile b/seqtk/Dockerfile
new file mode 100644
index 0000000..6d9210b
--- /dev/null
+++ b/seqtk/Dockerfile
@@ -0,0 +1,26 @@
+# DOCKERFILE for seqtk
+FROM ubuntu:16.04
+MAINTAINER Pedro Cerqueira, pedro.cerqueira@medicina.ulisboa.pt
+
+
+WORKDIR /NGStools/
+
+#Update and install dependencies
+RUN apt-get update && apt-get -y install \
+	bash \
+    build-essential \
+    make \
+    git \
+    zlib1g-dev
+
+#Clone seqtk from GitHub repo
+RUN git clone https://github.com/lh3/seqtk.git
+
+WORKDIR /NGStools/seqtk
+
+#Build seqtk
+RUN make
+
+#Add seqtk to 
+ENV PATH="/NGStools/seqtk:$PATH"
+