molecprot_gonad_buffer.qmd

# Changing Gonad Buffer

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Protocol for changing the ethanol out of gonad tissue vials.

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We are switching to a 95Etoh-PureNoAdditives buffer.
See [types of ethanol page](https://github.com/DrK-Lo/lotterhoslabprotocols/blob/3689d797871137dc6ae6eedd4959b8365b184d0c/molecprot_etoh) to understand why we are changing the ethanol out of gonad vials.

## Steps for changing ethanol out of gonad tissue vials

1. Centrigue the vial for 2 minutes at maximum speed
2. Use a pipette, suck up the 95MGetoh ethanol buffer

**Note**: Make sure to not suck up any tissue!

3. Add the 95Etoh-PureNoAdditives ethanol buffer

## Exceptions

### For cryovials that do not fit in the certifuge

1. Transfer the tissue into a labeled o-ring for long term storage

2. Then do the above steps!


### For vials with too much gonad and not enough ethanol (over 1:5 tissue-to-ethanol)

You may remove some of the tissue from the vial, but make sure there is at least 1:5 tissue-to-ethanol.