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The genome sequence was: TCGAGAGGC..., so the first CG positions were 2 and 3. Genome wide CpG reports may report the methylation state of a CG on the top strand starting at position 1, but not for the C on the reverse strand at position 2 because it can't determine a tri-nucleotide context. Thus we discard only the first line and read in a new one if the position of the first C was 1.
Now when the option --zero is specified however the first cytosines are reported as positions 1 and 2, which triggers the above correction behaviour, which I have now changed to take the 0-based coords into account. The resulting coverage file was also changed to now use 0-based genomic start and 1-based genomic end coordinates (zero-based, half-open), like used in the bedGraph file, like so:
"I'm having some problems with
coverage2cytosine
.It's terminating with exit code 25, and at the end of the output are the lines:
he strand of line 1 and line 2 were not + and -:
This is an analysis of spike in methylation controls and so the genome is very small.
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