diff --git a/Modules/ThirdParty/HDF5/src/itkhdf5/src/CMakeLists.txt b/Modules/ThirdParty/HDF5/src/itkhdf5/src/CMakeLists.txt
index 456347a79d3..253e0fb09aa 100644
--- a/Modules/ThirdParty/HDF5/src/itkhdf5/src/CMakeLists.txt
+++ b/Modules/ThirdParty/HDF5/src/itkhdf5/src/CMakeLists.txt
@@ -952,7 +952,6 @@ if (NOT EXISTS "${HDF5_GENERATED_SOURCE_DIR}/H5Tinit.c")
   TARGET_C_PROPERTIES (H5detect STATIC)
   target_link_libraries (H5detect
       PRIVATE "$<$<BOOL:${HDF5_ENABLE_PARALLEL}>:${MPI_C_LIBRARIES}>" $<$<OR:$<PLATFORM_ID:Windows>,$<PLATFORM_ID:MinGW>>:ws2_32.lib>
-      PRIVATE $<$<PLATFORM_ID:Emscripten>:"-O0">
   )
 
   if (HDF5_BATCH_H5DETECT)
@@ -1043,7 +1042,6 @@ target_compile_definitions(H5make_libsettings PUBLIC ${HDF_EXTRA_C_FLAGS} ${HDF_
 TARGET_C_PROPERTIES (H5make_libsettings STATIC)
 target_link_libraries (H5make_libsettings
     PRIVATE "$<$<BOOL:${HDF5_ENABLE_PARALLEL}>:${MPI_C_LIBRARIES}>" $<$<OR:$<PLATFORM_ID:Windows>,$<PLATFORM_ID:MinGW>>:ws2_32.lib>
-    PRIVATE $<$<PLATFORM_ID:Emscripten>:"-O0">
 )
 
 add_custom_command (