diff --git a/Marlin/src/inc/SanityCheck.h b/Marlin/src/inc/SanityCheck.h
index 59a74411ef7d..7662d604259b 100644
--- a/Marlin/src/inc/SanityCheck.h
+++ b/Marlin/src/inc/SanityCheck.h
@@ -2807,6 +2807,10 @@ static_assert(   _ARR_TEST(3,0) && _ARR_TEST(3,1) && _ARR_TEST(3,2)
   #endif
 #endif
 
+#if BOTH(Z_STEPPER_AUTO_ALIGN, MECHANICAL_GANTRY_CALIBRATION)
+  #error "You cannot use Z_STEPPER_AUTO_ALIGN and MECHANICAL_GANTRY_CALIBRATION at the same time."
+#endif
+
 #if ENABLED(PRINTCOUNTER) && DISABLED(EEPROM_SETTINGS)
   #error "PRINTCOUNTER requires EEPROM_SETTINGS. Please update your Configuration."
 #endif