diff --git a/Marlin/src/gcode/sd/M1001.cpp b/Marlin/src/gcode/sd/M1001.cpp
index 406cd074c3c2..8e24b60493f5 100644
--- a/Marlin/src/gcode/sd/M1001.cpp
+++ b/Marlin/src/gcode/sd/M1001.cpp
@@ -65,7 +65,7 @@
  */
 void GcodeSuite::M1001() {
   // If there's another auto#.g file to run...
-  if (TERN(NO_SD_AUTOSTART, false, card.autofile_check())) return;
+  if (TERN(NO_SD_AUTOSTART, false, !card.autofile_check())) return;
 
   // Purge the recovery file...
   TERN_(POWER_LOSS_RECOVERY, recovery.purge());