diff --git a/pkgs/applications/networking/browsers/qutebrowser/default.nix b/pkgs/applications/networking/browsers/qutebrowser/default.nix
index 1f898ed879867f2..4a9d1bd2ec27836 100644
--- a/pkgs/applications/networking/browsers/qutebrowser/default.nix
+++ b/pkgs/applications/networking/browsers/qutebrowser/default.nix
@@ -1,4 +1,4 @@
-{ stdenv, lib, fetchurl, fetchzip, python3
+{ stdenv, lib, fetchurl, fetchzip, fetchFromGitHub, python3
 , wrapQtAppsHook, glib-networking
 , asciidoc, docbook_xml_dtd_45, docbook_xsl, libxml2
 , libxslt, gst_all_1 ? null
@@ -8,14 +8,16 @@
 , pipewireSupport    ? stdenv.isLinux
 , pipewire
 , qtwayland
-, qtbase
-, qtwebengine
-, wrapGAppsHook
+, mkDerivationWith ? null
+, qtbase ? null
+, qtwebengine ? null
+, wrapGAppsHook ? null
 , enableWideVine ? false
 , widevine-cdm
-}:
+}: let
+  isQt6 = mkDerivationWith == null;
 
-let
+  python3Packages = python3.pkgs;
   pdfjs = let
     version = "3.9.179";
   in
@@ -25,21 +27,32 @@ let
     stripRoot = false;
   };
 
+  backendPackage =
+   if backend == "webengine" then if isQt6 then python3Packages.pyqt6-webengine else python3Packages.pyqtwebengine else
+   if backend == "webkit"    then python3Packages.pyqt5_with_qtwebkit else
+   throw ''
+     Unknown qutebrowser backend "${backend}".
+     Valid choices are qtwebengine (recommended) or qtwebkit.
+   '';
+
+  buildPythonApplication = if isQt6 then python3Packages.buildPythonApplication else mkDerivationWith python3Packages.buildPythonApplication;
+
   pname = "qutebrowser";
-  version = "3.0.0";
+  version = if isQt6 then "3.0.0" else "2.5.4";
 in
 
 assert withMediaPlayback -> gst_all_1 != null;
-assert lib.assertMsg (backend != "webkit") ''
-  Support for the QtWebKit backend has been removed.
-  Please remove the `backend = "webkit"` option from your qutebrowser override.
-'';
+assert isQt6 -> backend != "webkit";
 
-python3.pkgs.buildPythonApplication {
+buildPythonApplication {
   inherit pname version;
+
   src = fetchurl {
     url = "https://github.com/qutebrowser/qutebrowser/releases/download/v${version}/${pname}-${version}.tar.gz";
-    hash = "sha256-Oer0p/DwUfOejUCgSCSkMvLLAjNyJx51qgN7bcQQ2Pw=";
+    hash = {
+      "2.5.4" = "sha256-pGCyICUn5CpnDCbSJdn6ZBfQkswfFvOpXnvJXdicGrE=";
+      "3.0.0" = "sha256-Oer0p/DwUfOejUCgSCSkMvLLAjNyJx51qgN7bcQQ2Pw=";
+    }."${version}";
   };
 
   # Needs tox
@@ -56,18 +69,20 @@ python3.pkgs.buildPythonApplication {
   nativeBuildInputs = [
     wrapQtAppsHook wrapGAppsHook asciidoc
     docbook_xml_dtd_45 docbook_xsl libxml2 libxslt
-    python3.pkgs.pygments
-  ];
+  ]
+    ++ lib.optional isQt6 python3Packages.pygments;
 
   propagatedBuildInputs = with python3.pkgs; ([
-    pyyaml pyqt6-webengine jinja2 pygments
+    pyyaml backendPackage jinja2 pygments
     # scripts and userscripts libs
     tldextract beautifulsoup4
     readability-lxml pykeepass stem
     pynacl
     # extensive ad blocking
     adblock
-  ] ++ lib.optional stdenv.isLinux qtwayland
+  ]
+    ++ lib.optional (pythonOlder "3.9") importlib-resources
+    ++ lib.optional stdenv.isLinux qtwayland
   );
 
   patches = [
@@ -77,6 +92,10 @@ python3.pkgs.buildPythonApplication {
   dontWrapGApps = true;
   dontWrapQtApps = true;
 
+  preConfigure = lib.optionalString isQt6 ''
+    python scripts/asciidoc2html.py
+  '';
+
   postPatch = ''
     substituteInPlace qutebrowser/misc/quitter.py --subst-var-by qutebrowser "$out/bin/qutebrowser"
 
@@ -114,7 +133,10 @@ python3.pkgs.buildPythonApplication {
     makeWrapperArgs+=(
       "''${gappsWrapperArgs[@]}"
       "''${qtWrapperArgs[@]}"
-      ${lib.optionalString pipewireSupport ''--prefix LD_LIBRARY_PATH : ${libPath}''}
+      --add-flags '--backend ${backend}'
+      --set QUTE_QTWEBENGINE_VERSION_OVERRIDE "${lib.getVersion qtwebengine}"
+      ${lib.optionalString isQt6 ''--set QUTE_QT_WRAPPER "PyQt6"''}
+      ${lib.optionalString (pipewireSupport && backend == "webengine") ''--prefix LD_LIBRARY_PATH : ${libPath}''}
       ${lib.optionalString enableWideVine ''--add-flags "--qt-flag widevine-path=${widevine-cdm}/share/google/chrome/WidevineCdm/_platform_specific/linux_x64/libwidevinecdm.so"''}
     )
   '';
@@ -123,7 +145,7 @@ python3.pkgs.buildPythonApplication {
     homepage    = "https://github.com/qutebrowser/qutebrowser";
     description = "Keyboard-focused browser with a minimal GUI";
     license     = licenses.gpl3Plus;
-    platforms   = if enableWideVine then [ "x86_64-linux" ] else qtwebengine.meta.platforms;
+    platforms   = if enableWideVine then [ "x86_64-linux" ] else backendPackage.meta.platforms;
     maintainers = with maintainers; [ jagajaga rnhmjoj ebzzry dotlambda nrdxp ];
   };
 }
diff --git a/pkgs/top-level/aliases.nix b/pkgs/top-level/aliases.nix
index 70b39b5c52b7245..3058ba1d47edb20 100644
--- a/pkgs/top-level/aliases.nix
+++ b/pkgs/top-level/aliases.nix
@@ -1492,7 +1492,6 @@ mapAliases ({
   quake3game = throw "'quake3game' has been renamed to/replaced by 'ioquake3'"; # Converted to throw 2022-02-22
   quaternion-git = throw "quaternion-git has been removed in favor of the stable version 'quaternion'"; # Added 2020-04-09
   quilter = throw "quilter has been removed from nixpkgs, as it was unmaintained"; # Added 2021-08-03
-  qutebrowser-qt6 = throw "'qutebrowser-qt6' has been replaced by 'qutebrowser', since the the qt5 version has been removed"; # Added 2023-08-19
   qvim = throw "qvim has been removed"; # Added 2020-08-31
   qweechat = throw "qweechat has been removed because it was broken"; # Added 2021-03-08
   qwt6 = throw "'qwt6' has been renamed to/replaced by 'libsForQt5.qwt'"; # Converted to throw 2022-02-22
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index 4faf66e946a4f83..6df3addccd7bb6f 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -35109,7 +35109,8 @@ with pkgs;
     withXineBackend = true;
   };
 
-  qutebrowser = callPackage ../applications/networking/browsers/qutebrowser {
+  qutebrowser = libsForQt5.callPackage ../applications/networking/browsers/qutebrowser { };
+  qutebrowser-qt6 = callPackage ../applications/networking/browsers/qutebrowser {
     inherit (qt6Packages) qtbase qtwebengine wrapQtAppsHook qtwayland;
   };