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Recommendation for tools to detect tandem repeats? #233

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bl24 opened this issue Nov 13, 2019 · 1 comment
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Recommendation for tools to detect tandem repeats? #233

bl24 opened this issue Nov 13, 2019 · 1 comment

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@bl24
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bl24 commented Nov 13, 2019

I'm currently trying out pbsv on some mouse data that are aligned to multiple reference genomes that don't have tandem repeat annotations and I was wondering if y'all have recommendations for tools to generate a tandem repeat bed file?

@ylipacbio
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You may use Gary Benson's Tandem Repeat Finder, TRFD public database or download TRF tool and run on mouse reference genome.

Or alternatively if you have already installed SMRTLink, you may use

python \
 -m pbsvtools.tasks.split_ref_to_chrs \
 mouse.fasta \
 chr_size.csv

python \
      -m pbsvtools.tasks.tandem_repeat_finder \
      mouse.fasta \
      chr_size.csv \
      mouse.tandemrepeats.bed

File mouse.tandemrepeats.bed will contain tandem repeats and you may use it as input for pbsv discover option --tandem-repeats

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