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Custome genome DB: root.py not generating ' RD parity' related stats beyond Chr1 #250

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AnkiGurao opened this issue Jan 1, 2025 · 2 comments
Closed

Custome genome DB: root.py not generating ' RD parity' related stats beyond Chr1 #250

AnkiGurao opened this issue Jan 1, 2025 · 2 comments

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@AnkiGurao
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AnkiGurao commented Jan 1, 2025 •
edited by arpanda
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Hello dear Suvakov,
We are using CNVpytor for a custom genome for which we have successfully incorporated the genome reference in the db. But, the root.py not generating the RD related statistics beyond Chromosome 1. We checked the .bam file, its header, index, etc, everything is fine at the side of input file as well as the custom genome's reference in the db. I put the log here for your reference. Kindly suggest the solution to enable stats generation for all 31 chromosomes. Thanks.

**cnvpytor -root J9_new.pytor -rg BosGru -rd J9_new.bam**
2025-01-01 15:52:37,813 - cnvpytor.bam - INFO - File: J9_new.bam successfully open
2025-01-01 15:52:37,813 - cnvpytor.bam - INFO - Detected reference genome: BosGru
2025-01-01 15:52:37,835 - cnvpytor.pool - INFO - Parallel processing using 8 cores
2025-01-01 15:52:37,869 - cnvpytor.root - INFO - Reading data for chromosome CM016690.1 with length 172394104
2025-01-01 15:52:37,870 - cnvpytor.root - INFO - Reading data for chromosome CM016691.1 with length 150292554
2025-01-01 15:52:37,870 - cnvpytor.root - INFO - Reading data for chromosome CM016692.1 with length 132314468
2025-01-01 15:52:37,870 - cnvpytor.root - INFO - Reading data for chromosome CM016693.1 with length 130906049
2025-01-01 15:52:37,871 - cnvpytor.root - INFO - Reading data for chromosome CM016694.1 with length 129744616
2025-01-01 15:52:37,871 - cnvpytor.root - INFO - Reading data for chromosome CM016695.1 with length 128857574
2025-01-01 15:52:37,871 - cnvpytor.root - INFO - Reading data for chromosome CM016696.1 with length 124231334
2025-01-01 15:52:37,871 - cnvpytor.root - INFO - Reading data for chromosome CM016697.1 with length 120916904
2025-01-01 15:52:37,985 - cnvpytor.root - INFO - Reading data for chromosome CM016698.1 with length 118901860
2025-01-01 15:52:38,004 - cnvpytor.root - INFO - Reading data for chromosome CM016699.1 with length 114386978
2025-01-01 15:52:38,005 - cnvpytor.root - INFO - Reading data for chromosome CM016700.1 with length 96155913
2025-01-01 15:52:38,005 - cnvpytor.root - INFO - Reading data for chromosome CM016701.1 with length 92118950
2025-01-01 15:52:38,005 - cnvpytor.root - INFO - Reading data for chromosome CM016702.1 with length 90184876
2025-01-01 15:52:38,005 - cnvpytor.root - INFO - Reading data for chromosome CM016703.1 with length 89190698
2025-01-01 15:52:38,006 - cnvpytor.root - INFO - Reading data for chromosome CM016704.1 with length 85772466
2025-01-01 15:52:38,006 - cnvpytor.root - INFO - Reading data for chromosome CM016705.1 with length 81269117
2025-01-01 15:52:38,008 - cnvpytor.root - INFO - Reading data for chromosome CM016706.1 with length 77378949
2025-01-01 15:52:38,008 - cnvpytor.root - INFO - Reading data for chromosome CM016707.1 with length 75871366
2025-01-01 15:52:38,009 - cnvpytor.root - INFO - Reading data for chromosome CM016708.1 with length 72317570
2025-01-01 15:52:38,011 - cnvpytor.root - INFO - Reading data for chromosome CM016709.1 with length 71407976
2025-01-01 15:52:38,011 - cnvpytor.root - INFO - Reading data for chromosome CM016710.1 with length 68964392
2025-01-01 15:52:38,011 - cnvpytor.root - INFO - Reading data for chromosome CM016711.1 with length 66286744
2025-01-01 15:52:38,012 - cnvpytor.root - INFO - Reading data for chromosome CM016712.1 with length 60845766
2025-01-01 15:52:38,012 - cnvpytor.root - INFO - Reading data for chromosome CM016713.1 with length 57701545
2025-01-01 15:52:38,012 - cnvpytor.root - INFO - Reading data for chromosome CM016714.1 with length 57396858
2025-01-01 15:52:38,013 - cnvpytor.root - INFO - Reading data for chromosome CM016716.1 with length 50808821
2025-01-01 15:52:38,015 - cnvpytor.root - INFO - Reading data for chromosome CM016717.1 with length 49703996
2025-01-01 15:52:38,016 - cnvpytor.root - INFO - Reading data for chromosome CM016715.1 with length 48019877
2025-01-01 15:52:38,017 - cnvpytor.root - INFO - Reading data for chromosome CM016718.1 with length 46153303
2025-01-01 15:52:38,017 - cnvpytor.root - INFO - Reading data for chromosome CM016719.1 with length 136336377
2025-01-01 15:52:38,017 - cnvpytor.root - INFO - Reading data for chromosome CM016720.1 with length 26357969
2025-01-01 15:53:11,933 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016690.1'.
2025-01-01 15:53:14,197 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016691.1'.
2025-01-01 15:53:14,302 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016692.1'.
2025-01-01 15:53:14,407 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016693.1'.
2025-01-01 15:53:14,505 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016694.1'.
2025-01-01 15:53:14,601 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016695.1'.
2025-01-01 15:53:14,694 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016696.1'.
2025-01-01 15:53:14,788 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016697.1'.
2025-01-01 15:53:14,883 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016698.1'.
2025-01-01 15:53:14,969 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016699.1'.
2025-01-01 15:53:15,058 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016700.1'.
2025-01-01 15:53:15,130 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016701.1'.
2025-01-01 15:53:15,186 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016702.1'.
2025-01-01 15:53:15,242 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016703.1'.
2025-01-01 15:53:15,303 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016704.1'.
2025-01-01 15:53:15,362 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016705.1'.
2025-01-01 15:53:15,459 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016706.1'.
2025-01-01 15:53:15,522 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016707.1'.
2025-01-01 15:53:15,586 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016708.1'.
2025-01-01 15:53:15,648 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016709.1'.
2025-01-01 15:53:15,709 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016710.1'.
2025-01-01 15:53:15,765 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016711.1'.
2025-01-01 15:53:15,811 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016712.1'.
2025-01-01 15:53:15,849 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016713.1'.
2025-01-01 15:53:15,888 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016714.1'.
2025-01-01 15:53:15,933 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016716.1'.
2025-01-01 15:53:15,972 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016717.1'.
2025-01-01 15:53:16,011 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016715.1'.
2025-01-01 15:53:16,049 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016718.1'.
2025-01-01 15:53:16,087 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016719.1'.
2025-01-01 15:53:16,172 - cnvpytor.io - INFO - Saving chromosome RD data for chromosome 'CM016720.1'.
2025-01-01 15:53:16,497 - cnvpytor.root - INFO - GC content for reference genome 'BosGru' found in database.
2025-01-01 15:53:17,036 - cnvpytor.root - INFO - Chromosome 'CM016690.1' stat - RD parity distribution gaussian fit:  3.84 +- 4.37
2025-01-01 15:53:17,036 - cnvpytor.root - INFO - Chromosome 'CM016690.1' stat - RD unique distribution gaussian fit:  4.03 +- 4.21
2025-01-01 15:53:17,117 - cnvpytor.root - INFO - _Chromosome 'CM016691.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00_
2025-01-01 15:53:17,117 - cnvpytor.root - INFO - _Chromosome 'CM016691.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00_
2025-01-01 15:53:17,169 - cnvpytor.root - INFO - Chromosome 'CM016692.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,169 - cnvpytor.root - INFO - Chromosome 'CM016692.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,219 - cnvpytor.root - INFO - Chromosome 'CM016693.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,219 - cnvpytor.root - INFO - Chromosome 'CM016693.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,269 - cnvpytor.root - INFO - Chromosome 'CM016694.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,269 - cnvpytor.root - INFO - Chromosome 'CM016694.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,321 - cnvpytor.root - INFO - Chromosome 'CM016695.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,322 - cnvpytor.root - INFO - Chromosome 'CM016695.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,369 - cnvpytor.root - INFO - Chromosome 'CM016696.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,369 - cnvpytor.root - INFO - Chromosome 'CM016696.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,418 - cnvpytor.root - INFO - Chromosome 'CM016697.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,418 - cnvpytor.root - INFO - Chromosome 'CM016697.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,464 - cnvpytor.root - INFO - Chromosome 'CM016698.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,464 - cnvpytor.root - INFO - Chromosome 'CM016698.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,507 - cnvpytor.root - INFO - Chromosome 'CM016699.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,507 - cnvpytor.root - INFO - Chromosome 'CM016699.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,545 - cnvpytor.root - INFO - Chromosome 'CM016700.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,545 - cnvpytor.root - INFO - Chromosome 'CM016700.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,581 - cnvpytor.root - INFO - Chromosome 'CM016701.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,581 - cnvpytor.root - INFO - Chromosome 'CM016701.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,615 - cnvpytor.root - INFO - Chromosome 'CM016702.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,615 - cnvpytor.root - INFO - Chromosome 'CM016702.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,649 - cnvpytor.root - INFO - Chromosome 'CM016703.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,649 - cnvpytor.root - INFO - Chromosome 'CM016703.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,681 - cnvpytor.root - INFO - Chromosome 'CM016704.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,681 - cnvpytor.root - INFO - Chromosome 'CM016704.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,713 - cnvpytor.root - INFO - Chromosome 'CM016705.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,713 - cnvpytor.root - INFO - Chromosome 'CM016705.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,743 - cnvpytor.root - INFO - Chromosome 'CM016706.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,743 - cnvpytor.root - INFO - Chromosome 'CM016706.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,771 - cnvpytor.root - INFO - Chromosome 'CM016707.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,771 - cnvpytor.root - INFO - Chromosome 'CM016707.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,801 - cnvpytor.root - INFO - Chromosome 'CM016708.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,801 - cnvpytor.root - INFO - Chromosome 'CM016708.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,828 - cnvpytor.root - INFO - Chromosome 'CM016709.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,828 - cnvpytor.root - INFO - Chromosome 'CM016709.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,853 - cnvpytor.root - INFO - Chromosome 'CM016710.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,854 - cnvpytor.root - INFO - Chromosome 'CM016710.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,879 - cnvpytor.root - INFO - Chromosome 'CM016711.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,879 - cnvpytor.root - INFO - Chromosome 'CM016711.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,903 - cnvpytor.root - INFO - Chromosome 'CM016712.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,903 - cnvpytor.root - INFO - Chromosome 'CM016712.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,925 - cnvpytor.root - INFO - Chromosome 'CM016713.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,925 - cnvpytor.root - INFO - Chromosome 'CM016713.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,947 - cnvpytor.root - INFO - Chromosome 'CM016714.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,947 - cnvpytor.root - INFO - Chromosome 'CM016714.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,965 - cnvpytor.root - INFO - Chromosome 'CM016715.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,966 - cnvpytor.root - INFO - Chromosome 'CM016715.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,985 - cnvpytor.root - INFO - Chromosome 'CM016716.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,985 - cnvpytor.root - INFO - Chromosome 'CM016716.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:18,003 - cnvpytor.root - INFO - Chromosome 'CM016717.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:18,004 - cnvpytor.root - INFO - Chromosome 'CM016717.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:18,021 - cnvpytor.root - INFO - Chromosome 'CM016718.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:18,021 - cnvpytor.root - INFO - Chromosome 'CM016718.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:18,074 - cnvpytor.root - INFO - Chromosome 'CM016719.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:18,074 - cnvpytor.root - INFO - Chromosome 'CM016719.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:18,085 - cnvpytor.root - INFO - Chromosome 'CM016720.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:18,085 - cnvpytor.root - INFO - Chromosome 'CM016720.1' stat - RD unique distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:18,085 - cnvpytor.root - INFO - Chromosome 'CM016690.1' stat 
2025-01-01 15:53:18,439 - cnvpytor.root - INFO - Chromosome 'CM016691.1' stat 
2025-01-01 15:53:18,625 - cnvpytor.root - INFO - Chromosome 'CM016692.1' stat 
2025-01-01 15:53:18,781 - cnvpytor.root - INFO - Chromosome 'CM016693.1' stat 
2025-01-01 15:53:18,935 - cnvpytor.root - INFO - Chromosome 'CM016694.1' stat 
2025-01-01 15:53:19,088 - cnvpytor.root - INFO - Chromosome 'CM016695.1' stat 
2025-01-01 15:53:19,237 - cnvpytor.root - INFO - Chromosome 'CM016696.1' stat 
2025-01-01 15:53:19,385 - cnvpytor.root - INFO - Chromosome 'CM016697.1' stat 
2025-01-01 15:53:19,525 - cnvpytor.root - INFO - Chromosome 'CM016698.1' stat 
2025-01-01 15:53:19,663 - cnvpytor.root - INFO - Chromosome 'CM016699.1' stat 
2025-01-01 15:53:19,794 - cnvpytor.root - INFO - Chromosome 'CM016700.1' stat 
2025-01-01 15:53:19,907 - cnvpytor.root - INFO - Chromosome 'CM016701.1' stat 
2025-01-01 15:53:20,016 - cnvpytor.root - INFO - Chromosome 'CM016702.1' stat 
2025-01-01 15:53:20,121 - cnvpytor.root - INFO - Chromosome 'CM016703.1' stat 
2025-01-01 15:53:20,223 - cnvpytor.root - INFO - Chromosome 'CM016704.1' stat 
2025-01-01 15:53:20,323 - cnvpytor.root - INFO - Chromosome 'CM016705.1' stat 
2025-01-01 15:53:20,420 - cnvpytor.root - INFO - Chromosome 'CM016706.1' stat 
2025-01-01 15:53:20,515 - cnvpytor.root - INFO - Chromosome 'CM016707.1' stat 
2025-01-01 15:53:20,604 - cnvpytor.root - INFO - Chromosome 'CM016708.1' stat 
2025-01-01 15:53:20,689 - cnvpytor.root - INFO - Chromosome 'CM016709.1' stat 
2025-01-01 15:53:20,773 - cnvpytor.root - INFO - Chromosome 'CM016710.1' stat 
2025-01-01 15:53:20,852 - cnvpytor.root - INFO - Chromosome 'CM016711.1' stat 
2025-01-01 15:53:20,931 - cnvpytor.root - INFO - Chromosome 'CM016712.1' stat 
2025-01-01 15:53:21,026 - cnvpytor.root - INFO - Chromosome 'CM016713.1' stat 
2025-01-01 15:53:21,094 - cnvpytor.root - INFO - Chromosome 'CM016714.1' stat 
2025-01-01 15:53:21,164 - cnvpytor.root - INFO - Chromosome 'CM016715.1' stat 
2025-01-01 15:53:21,221 - cnvpytor.root - INFO - Chromosome 'CM016716.1' stat 
2025-01-01 15:53:21,279 - cnvpytor.root - INFO - Chromosome 'CM016717.1' stat 
2025-01-01 15:53:21,338 - cnvpytor.root - INFO - Chromosome 'CM016718.1' stat 
2025-01-01 15:53:21,392 - cnvpytor.root - INFO - Chromosome 'CM016719.1' stat 
2025-01-01 15:53:21,554 - cnvpytor.root - INFO - Chromosome 'CM016720.1' stat 
2025-01-01 15:53:21,587 - cnvpytor.root - INFO - Autosomes stat - RD parity distribution gaussian fit:  3.84 +- 4.37
2025-01-01 15:53:21,604 - cnvpytor.root - INFO - Sex chromosomes stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:21,618 - cnvpytor.root - INFO - Reference genome 'BosGru' found in database.
2025-01-01 15:53:21,620 - cnvpytor.root - INFO - GC content for reference genome 'BosGru' found in database.
@arpanda
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arpanda commented Jan 3, 2025

Hi @AnkiGurao,
CNVpytor is reporting that there is almost no read for 'CM016691.1', 'CM016692.1' , etc.

2025-01-01 15:53:17,169 - cnvpytor.root - INFO - Chromosome 'CM016692.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00
2025-01-01 15:53:17,219 - cnvpytor.root - INFO - Chromosome 'CM016693.1' stat - RD parity distribution gaussian fit:  0.00 +- 0.00

could you please check, if you have reads or not.

Thank you,
Arijit

@AnkiGurao
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Hello @arpanda
Thank you for the kind response. The BAM file was truncated, sorted the issue.

@arpanda arpanda closed this as completed Jan 3, 2025
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