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The amount of effort you put in juicer looks really incredible. Being a total newbie, I try to run juicer on Google Colab in CPU mode. And yeah, Google Colab is really a gold standard now, so I've supposed there would be no entanglements - just as there were no problems with the HiCCUPS colab referenced in the wiki (thanks!).
As you can infer, I was wrong.
Here is my Colab code:
# Install dependencies (we have Linux Ubuntu here)
!apt install samtools bwa
# Clone the repository
!git clone https://github.com/aidenlab/juicer.git
# Arrange generic folder structure
!mkdir references fastq restriction_sites aligned hic_results
# Get the examples
# Note the -P trick:
# it's not that easy to perform a long-playing `cd` in colab!
!wget -P references https://s3.amazonaws.com/juicerawsmirror/opt/juicer/references/Homo_sapiens_assembly19.fasta
!wget -P references https://s3.amazonaws.com/juicerawsmirror/opt/juicer/references/Homo_sapiens_assembly19.fasta.amb
!wget -P references https://s3.amazonaws.com/juicerawsmirror/opt/juicer/references/Homo_sapiens_assembly19.fasta.ann
!wget -P references https://s3.amazonaws.com/juicerawsmirror/opt/juicer/references/Homo_sapiens_assembly19.fasta.bwt
!wget -P references https://s3.amazonaws.com/juicerawsmirror/opt/juicer/references/Homo_sapiens_assembly19.fasta.pac
!wget -P references https://s3.amazonaws.com/juicerawsmirror/opt/juicer/references/Homo_sapiens_assembly19.fasta.sa
!wget -P restriction_sites https://s3.amazonaws.com/juicerawsmirror/opt/juicer/restriction_sites/hg19_MboI.txt
# In Colab, we run in CPU mode
!ln -s juicer/CPU scripts
# Do I need to add `/common/` ?..
!cd scripts; wget https://hicfiles.tc4ga.com/public/juicer/juicer_tools.1.9.9_jcuda.0.8.jar
!cd scripts; ln -s juicer_tools.1.9.9_jcuda.0.8.jar juicer_tools.jar
# It insists...
!rm -rf aligned
!./scripts/juicer.sh -D `pwd` -g "hg19"
Dear AidenLab,
The amount of effort you put in
juicer
looks really incredible. Being a total newbie, I try to runjuicer
on Google Colab in CPU mode. And yeah, Google Colab is really a gold standard now, so I've supposed there would be no entanglements - just as there were no problems with the HiCCUPS colab referenced in the wiki (thanks!).As you can infer, I was wrong.
Here is my Colab code:
It is based on https://github.com/aidenlab/juicer/wiki/Installation
I get the following output:
Details
What am I doing wrong?
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