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Importing float .mtx files (e.g., like those from multimapping) in R for downstream analysis is currently not straightforward. Would there be any limitations if STAR just output these files as real instead?
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did you mean integer instead of float?
When multimappers are included, the counts indeed become non-integer, as one read can be "distributed" between multiple genes. You can round them to integeres, but is not the best approach, I believe - see the discussion here: #1262 (comment)
No -- i meant real instead of float. It appears if you simply change the header in the .mtx from float to real, the .mtx files can be read into R by the various packages (most of which appear to depend on the Matrix R package?). Is there any limitation with only real precision vs. float that you can imagine?
Importing float .mtx files (e.g., like those from multimapping) in R for downstream analysis is currently not straightforward. Would there be any limitations if STAR just output these files as real instead?
The text was updated successfully, but these errors were encountered: