diff --git a/cli/tests/unit/wasm_test.ts b/cli/tests/unit/wasm_test.ts
index e1659b090b8388..3484b7f818b581 100644
--- a/cli/tests/unit/wasm_test.ts
+++ b/cli/tests/unit/wasm_test.ts
@@ -76,6 +76,20 @@ Deno.test(async function wasmInstantiateStreaming() {
   assertEquals(add(1, 3), 4);
 });
 
+Deno.test(
+  { permissions: { read: true } },
+  async function wasmFileStreaming() {
+    const url = new URL("../testdata/unreachable.wasm", import.meta.url);
+    assert(url.href.startsWith("file://"));
+
+    const { module } = await WebAssembly.instantiateStreaming(fetch(url));
+    assertEquals(WebAssembly.Module.exports(module), [{
+      name: "unreachable",
+      kind: "function",
+    }]);
+  },
+);
+
 Deno.test(
   { permissions: { net: true } },
   async function wasmStreamingNonTrivial() {
diff --git a/ext/fetch/26_fetch.js b/ext/fetch/26_fetch.js
index 47d8b2e8598071..a66146bee57381 100644
--- a/ext/fetch/26_fetch.js
+++ b/ext/fetch/26_fetch.js
@@ -37,6 +37,7 @@
     PromisePrototypeThen,
     PromisePrototypeCatch,
     String,
+    StringPrototypeStartsWith,
     StringPrototypeToLowerCase,
     TypedArrayPrototypeSubarray,
     TypeError,
@@ -498,12 +499,16 @@
         // The spec is ambiguous here, see
         // https://github.com/WebAssembly/spec/issues/1138. The WPT tests
         // expect the raw value of the Content-Type attribute lowercased.
-        const contentType = res.headers.get("Content-Type");
-        if (
-          typeof contentType !== "string" ||
-          StringPrototypeToLowerCase(contentType) !== "application/wasm"
-        ) {
-          throw new TypeError("Invalid WebAssembly content type.");
+        // We ignore this for file:// because file fetches don't have a
+        // Content-Type.
+        if (!StringPrototypeStartsWith(res.url, "file://")) {
+          const contentType = res.headers.get("Content-Type");
+          if (
+            typeof contentType !== "string" ||
+            StringPrototypeToLowerCase(contentType) !== "application/wasm"
+          ) {
+            throw new TypeError("Invalid WebAssembly content type.");
+          }
         }
 
         // 2.5.