diff --git a/packages/amplify-category-function/src/provider-utils/awscloudformation/utils/layerHelpers.ts b/packages/amplify-category-function/src/provider-utils/awscloudformation/utils/layerHelpers.ts
index 53218a124e7..9e0aec61689 100644
--- a/packages/amplify-category-function/src/provider-utils/awscloudformation/utils/layerHelpers.ts
+++ b/packages/amplify-category-function/src/provider-utils/awscloudformation/utils/layerHelpers.ts
@@ -21,6 +21,8 @@ const layerResourceGlobs = [parametersFileName, `*${cfnTemplateSuffix}`];
 const libPathName = 'lib';
 const optPathName = 'opt';
 const packageJson = 'package.json';
+const pipfile = 'Pipfile';
+const pipfileLock = 'Pipfile.lock';
 
 export interface LayerInputParams {
   layerPermissions?: PermissionEnum[];
@@ -343,7 +345,28 @@ const getLayerGlobs = async (
 
     result.push(...contentFilePaths);
   } else if (runtimeId === 'python') {
-    // No special treatment needed for python yet.
+    // Add to hashable files/folders
+    const pipfileFilePath = path.join(layerCodePath, pipfile);
+
+    if (fs.existsSync(pipfileFilePath)) {
+      result.push(path.join(libPathName, layerExecutablePath, pipfile));
+    }
+
+    // If lock file is present, add to hashable files/folders
+    const pipfileLockFilePath = path.join(layerCodePath, pipfileLock);
+
+    if (fs.existsSync(pipfileLockFilePath)) {
+      result.push(path.join(libPathName, layerExecutablePath, pipfileLockFilePath));
+    }
+
+    // Add layer direct content from lib/python and exclude well known files from list.
+    // files must be relative to resource folder as that will be used as a base path for hashing.
+    const contentFilePaths = await globby([path.join(libPathName, layerExecutablePath, '**', '*')], {
+      cwd: resourcePath,
+      ignore: ['lib', pipfile, pipfileLock].map(name => path.join(libPathName, layerExecutablePath, name)),
+    });
+
+    result.push(...contentFilePaths);
   } else {
     const error = new Error(`Unsupported layer runtime: ${runtimeId} for resource: ${resourceName}`);
     error.stack = undefined;