diff --git a/packages/aws-cdk-lib/aws-s3/lib/bucket.ts b/packages/aws-cdk-lib/aws-s3/lib/bucket.ts
index df198dafe9500..9db166b0beb6d 100644
--- a/packages/aws-cdk-lib/aws-s3/lib/bucket.ts
+++ b/packages/aws-cdk-lib/aws-s3/lib/bucket.ts
@@ -2119,6 +2119,11 @@ export class Bucket extends BucketBase {
 
     function parseLifecycleRule(rule: LifecycleRule): CfnBucket.RuleProperty {
       const enabled = rule.enabled ?? true;
+      if ((rule.expiredObjectDeleteMarker)
+      && (rule.expiration || rule.expirationDate || self.parseTagFilters(rule.tagFilters))) {
+        // ExpiredObjectDeleteMarker cannot be specified with ExpirationInDays, ExpirationDate, or TagFilters.
+        throw new Error('ExpiredObjectDeleteMarker cannot be specified with expiration, ExpirationDate, or TagFilters.');
+      }
 
       const x: CfnBucket.RuleProperty = {
         // eslint-disable-next-line max-len
diff --git a/packages/aws-cdk-lib/aws-s3/test/rules.test.ts b/packages/aws-cdk-lib/aws-s3/test/rules.test.ts
index b218a098d4ff4..25a45a6ea344f 100644
--- a/packages/aws-cdk-lib/aws-s3/test/rules.test.ts
+++ b/packages/aws-cdk-lib/aws-s3/test/rules.test.ts
@@ -25,6 +25,51 @@ describe('rules', () => {
     });
   });
 
+  test('ExpiredObjectDeleteMarker cannot be specified with ExpirationInDays.', () => {
+    const stack = new Stack();
+    new Bucket(stack, 'Bucket', {
+      lifecycleRules: [{
+        expiration: Duration.days(30),
+        expiredObjectDeleteMarker: true,
+      }],
+    });
+
+    expect(() => {
+      Template.fromStack(stack).toJSON();
+    }).toThrow('ExpiredObjectDeleteMarker cannot be specified with expiration, ExpirationDate, or TagFilters.');
+  });
+
+  test('ExpiredObjectDeleteMarker cannot be specified with ExpirationDate.', () => {
+    const stack = new Stack();
+    new Bucket(stack, 'Bucket', {
+      lifecycleRules: [{
+        expirationDate: new Date('2018-01-01'),
+        expiredObjectDeleteMarker: true,
+      }],
+    });
+
+    expect(() => {
+      Template.fromStack(stack).toJSON();
+    }).toThrow('ExpiredObjectDeleteMarker cannot be specified with expiration, ExpirationDate, or TagFilters.');
+  });
+
+  test('ExpiredObjectDeleteMarker cannot be specified with TagFilters.', () => {
+    const stack = new Stack();
+    new Bucket(stack, 'Bucket', {
+      lifecycleRules: [{
+        tagFilters: [
+          { Key: 'tagname1', Value: 'tagvalue1' },
+          { Key: 'tagname2', Value: 'tagvalue2' },
+        ],
+        expiredObjectDeleteMarker: true,
+      }],
+    });
+
+    expect(() => {
+      Template.fromStack(stack).toJSON();
+    }).toThrow('ExpiredObjectDeleteMarker cannot be specified with expiration, ExpirationDate, or TagFilters.');
+  });
+
   test('Can use addLifecycleRule() to add a lifecycle rule', () => {
     // GIVEN
     const stack = new Stack();