-
Notifications
You must be signed in to change notification settings - Fork 183
/
Copy pathWebDBSeqI.pm
888 lines (709 loc) · 25 KB
/
WebDBSeqI.pm
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
694
695
696
697
698
699
700
701
702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817
818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867
868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
#
# BioPerl module for Bio::DB::WebDBSeqI
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Jason Stajich <jason@bioperl.org>
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself
#
# POD documentation - main docs before the code
#
=head1 NAME
Bio::DB::WebDBSeqI - Object Interface to generalize Web Databases
for retrieving sequences
=head1 SYNOPSIS
# get a WebDBSeqI object somehow
# assuming it is a nucleotide db
my $seq = $db->get_Seq_by_id('ROA1_HUMAN')
=head1 DESCRIPTION
Provides core set of functionality for connecting to a web based
database for retrieving sequences.
Users wishing to add another Web Based Sequence Dabatase will need to
extend this class (see L<Bio::DB::SwissProt> or L<Bio::DB::NCBIHelper> for
examples) and implement the get_request method which returns a
HTTP::Request for the specified uids (accessions, ids, etc depending
on what query types the database accepts).
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the
evolution of this and other Bioperl modules. Send
your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation
is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to
help us keep track the bugs and their resolution.
Bug reports can be submitted via the web.
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHOR - Jason Stajich
Email E<lt> jason@bioperl.org E<gt>
=head1 APPENDIX
The rest of the documentation details each of the
object methods. Internal methods are usually
preceded with a _
=cut
# Let the code begin...
package Bio::DB::WebDBSeqI;
use strict;
use Carp;
use vars qw($MODVERSION %RETRIEVAL_TYPES $DEFAULT_RETRIEVAL_TYPE
$DEFAULTFORMAT $LAST_INVOCATION_TIME @ATTRIBUTES);
use Bio::SeqIO;
use Bio::Root::IO;
use LWP::UserAgent;
use POSIX 'setsid';
use HTTP::Request::Common;
use HTTP::Response;
use File::Spec;
use IO::Pipe;
use IO::String;
use Bio::Root::Root;
use base qw(Bio::DB::RandomAccessI);
BEGIN {
$MODVERSION = '0.8';
%RETRIEVAL_TYPES = ('io_string' => 1,
'tempfile' => 1,
'pipeline' => 1,
);
$DEFAULT_RETRIEVAL_TYPE = 'pipeline';
$DEFAULTFORMAT = 'fasta';
$LAST_INVOCATION_TIME = 0;
}
sub new {
my ($class, @args) = @_;
my $self = $class->SUPER::new(@args);
my ($baseaddress, $params, $ret_type, $format,$delay,$db) =
$self->_rearrange([qw(BASEADDRESS PARAMS RETRIEVALTYPE FORMAT DELAY DB)],
@args);
$ret_type = $DEFAULT_RETRIEVAL_TYPE unless ( $ret_type);
$baseaddress && $self->url_base_address($baseaddress);
$params && $self->url_params($params);
$db && $self->db($db);
$ret_type && $self->retrieval_type($ret_type);
$delay = $self->delay_policy unless defined $delay;
$self->delay($delay);
# insure we always have a default format set for retrieval
# even though this will be immedietly overwritten by most sub classes
$format = $self->default_format unless ( defined $format &&
$format ne '' );
$self->request_format($format);
my $ua = LWP::UserAgent->new(env_proxy => 1);
$ua->agent(ref($self) ."/$MODVERSION");
$self->ua($ua);
$self->{'_authentication'} = [];
return $self;
}
# from Bio::DB::RandomAccessI
=head2 get_Seq_by_id
Title : get_Seq_by_id
Usage : $seq = $db->get_Seq_by_id('ROA1_HUMAN')
Function: Gets a Bio::Seq object by its name
Returns : a Bio::Seq object
Args : the id (as a string) of a sequence
Throws : "id does not exist" exception
=cut
sub get_Seq_by_id {
my ($self,$seqid) = @_;
$self->_sleep;
my $seqio = $self->get_Stream_by_id([$seqid]);
$self->throw("id does not exist") if( !defined $seqio ) ;
if ($self->can('complexity') && defined $self->complexity && $self->complexity==0) {
$self->warn("When complexity is set to 0, use get_Stream_by_id\n".
"Returning Bio::SeqIO object");
return $seqio;
}
my @seqs;
while( my $seq = $seqio->next_seq() ) { push @seqs, $seq; }
# Since $seqio will not be used anymore, explicitly close its filehandle
# or it will cause trouble later on cleanup
$seqio->close;
$self->throw("id '$seqid' does not exist") unless @seqs;
if( wantarray ) { return @seqs } else { return shift @seqs }
}
=head2 get_Seq_by_acc
Title : get_Seq_by_acc
Usage : $seq = $db->get_Seq_by_acc('X77802');
Function: Gets a Bio::Seq object by accession number
Returns : A Bio::Seq object
Args : accession number (as a string)
Throws : "acc does not exist" exception
=cut
sub get_Seq_by_acc {
my ($self,$seqid) = @_;
$self->_sleep;
my $seqio = $self->get_Stream_by_acc($seqid);
$self->throw("acc '$seqid' does not exist") if( ! defined $seqio );
if ($self->can('complexity') && defined $self->complexity && $self->complexity==0) {
$self->warn("When complexity is set to 0, use get_Stream_by_acc\n".
"Returning Bio::SeqIO object");
return $seqio;
}
my @seqs;
while( my $seq = $seqio->next_seq() ) { push @seqs, $seq; }
$self->throw("acc $seqid does not exist") unless @seqs;
if( wantarray ) { return @seqs } else { return shift @seqs }
}
=head2 get_Seq_by_gi
Title : get_Seq_by_gi
Usage : $seq = $db->get_Seq_by_gi('405830');
Function: Gets a Bio::Seq object by gi number
Returns : A Bio::Seq object
Args : gi number (as a string)
Throws : "gi does not exist" exception
=cut
sub get_Seq_by_gi {
my ($self,$seqid) = @_;
$self->_sleep;
my $seqio = $self->get_Stream_by_gi($seqid);
$self->throw("gi does not exist") if( !defined $seqio );
if ($self->can('complexity') && defined $self->complexity && $self->complexity==0) {
$self->warn("When complexity is set to 0, use get_Stream_by_gi\n".
"Returning Bio::SeqIO object");
return $seqio;
}
my @seqs;
while( my $seq = $seqio->next_seq() ) { push @seqs, $seq; }
$self->throw("gi does not exist") unless @seqs;
if( wantarray ) { return @seqs } else { return shift @seqs }
}
=head2 get_Seq_by_version
Title : get_Seq_by_version
Usage : $seq = $db->get_Seq_by_version('X77802.1');
Function: Gets a Bio::Seq object by sequence version
Returns : A Bio::Seq object
Args : accession.version (as a string)
Throws : "acc.version does not exist" exception
=cut
sub get_Seq_by_version {
my ($self,$seqid) = @_;
$self->_sleep;
my $seqio = $self->get_Stream_by_version($seqid);
$self->throw("accession.version does not exist") if( !defined $seqio );
if ($self->can('complexity') && defined $self->complexity && $self->complexity==0) {
$self->warn("When complexity is set to 0, use get_Stream_by_version\n".
"Returning Bio::SeqIO object");
return $seqio;
}
my @seqs;
while( my $seq = $seqio->next_seq() ) { push @seqs, $seq; }
$self->throw("accession.version does not exist") unless @seqs;
if( wantarray ) { return @seqs } else { return shift @seqs }
}
# implementing class must define these
=head2 get_request
Title : get_request
Usage : my $url = $self->get_request
Function: returns a HTTP::Request object
Returns :
Args : %qualifiers = a hash of qualifiers (ids, format, etc)
=cut
sub get_request {
my ($self) = @_;
my $msg = "Implementing class must define method get_request in class WebDBSeqI";
$self->throw($msg);
}
# class methods
=head2 get_Stream_by_id
Title : get_Stream_by_id
Usage : $stream = $db->get_Stream_by_id( [$uid1, $uid2] );
Function: Gets a series of Seq objects by unique identifiers
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of unique identifiers for
the desired sequence entries
=cut
sub get_Stream_by_id {
my ($self, $ids) = @_;
my ($webfmt,$localfmt) = $self->request_format;
return $self->get_seq_stream('-uids' => $ids, '-mode' => 'single',
'-format' => $webfmt);
}
*get_Stream_by_batch = sub {
my $self = shift;
Carp::carp('get_Stream_by_batch() is deprecated; use get_Stream_by_id() instead');
$self->get_Stream_by_id(@_)
};
=head2 get_Stream_by_acc
Title : get_Stream_by_acc
Usage : $seq = $db->get_Stream_by_acc([$acc1, $acc2]);
Function: Gets a series of Seq objects by accession numbers
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of accession numbers for
the desired sequence entries
Note : For GenBank, this just calls the same code for get_Stream_by_id()
=cut
sub get_Stream_by_acc {
my ($self, $ids ) = @_;
return $self->get_seq_stream('-uids' => $ids, '-mode' => 'single');
}
=head2 get_Stream_by_gi
Title : get_Stream_by_gi
Usage : $seq = $db->get_Stream_by_gi([$gi1, $gi2]);
Function: Gets a series of Seq objects by gi numbers
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of gi numbers for
the desired sequence entries
Note : For GenBank, this just calls the same code for get_Stream_by_id()
=cut
sub get_Stream_by_gi {
my ($self, $ids ) = @_;
return $self->get_seq_stream('-uids' => $ids, '-mode' => 'gi');
}
=head2 get_Stream_by_version
Title : get_Stream_by_version
Usage : $seq = $db->get_Stream_by_version([$version1, $version2]);
Function: Gets a series of Seq objects by accession.versions
Returns : a Bio::SeqIO stream object
Args : $ref : a reference to an array of accession.version strings for
the desired sequence entries
Note : For GenBank, this is implemented in NCBIHelper
=cut
sub get_Stream_by_version {
my ($self, $ids ) = @_;
# $self->throw("Implementing class should define this method!");
return $self->get_seq_stream('-uids' => $ids, '-mode' => 'version'); # how it should work
}
=head2 get_Stream_by_query
Title : get_Stream_by_query
Usage : $stream = $db->get_Stream_by_query($query);
Function: Gets a series of Seq objects by way of a query string or oject
Returns : a Bio::SeqIO stream object
Args : $query : A string that uses the appropriate query language
for the database or a Bio::DB::QueryI object. It is suggested
that you create the Bio::DB::Query object first and interrogate
it for the entry count before you fetch a potentially large stream.
=cut
sub get_Stream_by_query {
my ($self, $query ) = @_;
return $self->get_seq_stream('-query' => $query, '-mode'=>'query');
}
=head2 default_format
Title : default_format
Usage : my $format = $self->default_format
Function: Returns default sequence format for this module
Returns : string
Args : none
=cut
sub default_format {
return $DEFAULTFORMAT;
}
# sorry, but this is hacked in because of BioFetch problems...
sub db {
my $self = shift;
my $d = $self->{_db};
$self->{_db} = shift if @_;
$d;
}
=head2 request_format
Title : request_format
Usage : my ($req_format, $ioformat) = $self->request_format;
$self->request_format("genbank");
$self->request_format("fasta");
Function: Get/Set sequence format retrieval. The get-form will normally not
be used outside of this and derived modules.
Returns : Array of two strings, the first representing the format for
retrieval, and the second specifying the corresponding SeqIO format.
Args : $format = sequence format
=cut
sub request_format {
my ($self, $value) = @_;
if( defined $value ) {
$self->{'_format'} = [ $value, $value];
}
return @{$self->{'_format'}};
}
=head2 get_seq_stream
Title : get_seq_stream
Usage : my $seqio = $self->get_seq_stream(%qualifiers)
Function: builds a url and queries a web db
Returns : a Bio::SeqIO stream capable of producing sequence
Args : %qualifiers = a hash qualifiers that the implementing class
will process to make a url suitable for web querying
=cut
sub get_seq_stream {
my ($self, %qualifiers) = @_;
my ($rformat, $ioformat) = $self->request_format();
my $seen = 0;
foreach my $key ( keys %qualifiers ) {
if( $key =~ /format/i ) {
$rformat = $qualifiers{$key};
$seen = 1;
}
}
$qualifiers{'-format'} = $rformat if( !$seen);
($rformat, $ioformat) = $self->request_format($rformat);
# These parameters are implemented for Bio::DB::GenBank objects only
if($self->isa('Bio::DB::GenBank')) {
$self->seq_start() && ($qualifiers{'-seq_start'} = $self->seq_start());
$self->seq_stop() && ($qualifiers{'-seq_stop'} = $self->seq_stop());
$self->strand() && ($qualifiers{'-strand'} = $self->strand());
defined $self->complexity() && ($qualifiers{'-complexity'} = $self->complexity());
}
my $request = $self->get_request(%qualifiers);
$request->proxy_authorization_basic($self->authentication)
if ( $self->authentication);
$self->debug("request is ". $request->as_string(). "\n");
# workaround for MSWin systems
$self->retrieval_type('io_string') if $self->retrieval_type =~ /pipeline/ && $^O =~ /^MSWin/;
if ($self->retrieval_type =~ /pipeline/) {
# Try to create a stream using POSIX fork-and-pipe facility.
# this is a *big* win when fetching thousands of sequences from
# a web database because we can return the first entry while
# transmission is still in progress.
# Also, no need to keep sequence in memory or in a temporary file.
# If this fails (Windows, MacOS 9), we fall back to non-pipelined access.
# fork and pipe: _stream_request()=><STREAM>
my ($result,$stream) = $self->_open_pipe();
if (defined $result) {
$DB::fork_TTY = File::Spec->devnull; # prevents complaints from debugger
if (!$result) { # in child process
$self->_stream_request($request,$stream);
POSIX::_exit(0); #prevent END blocks from executing in this forked child
}
else {
return Bio::SeqIO->new('-verbose' => $self->verbose,
'-format' => $ioformat,
'-fh' => $stream);
}
}
else {
$self->retrieval_type('io_string');
}
}
if ($self->retrieval_type =~ /temp/i) {
my $dir = $self->io->tempdir( CLEANUP => 1);
my ( $fh, $tmpfile) = $self->io()->tempfile( DIR => $dir );
close $fh;
my $resp = $self->_request($request, $tmpfile);
if( ! -e $tmpfile || -z $tmpfile || ! $resp->is_success() ) {
$self->throw("WebDBSeqI Error - check query sequences!\n");
}
$self->postprocess_data('type' => 'file',
'location' => $tmpfile);
# this may get reset when requesting batch mode
($rformat,$ioformat) = $self->request_format();
if( $self->verbose > 0 ) {
open my $ERR, '<', $tmpfile or $self->throw("Could not read file '$tmpfile': $!");
while(<$ERR>) { $self->debug($_);}
close $ERR;
}
return Bio::SeqIO->new('-verbose' => $self->verbose,
'-format' => $ioformat,
'-file' => $tmpfile);
}
if ($self->retrieval_type =~ /io_string/i ) {
my $resp = $self->_request($request);
my $content = $resp->content_ref;
$self->debug( "content is $$content\n");
if (!$resp->is_success() || length($$content) == 0) {
$self->throw("WebDBSeqI Error - check query sequences!\n");
}
($rformat,$ioformat) = $self->request_format();
$self->postprocess_data('type'=> 'string',
'location' => $content);
$self->debug( "str is $$content\n");
return Bio::SeqIO->new('-verbose' => $self->verbose,
'-format' => $ioformat,
'-fh' => new IO::String($$content));
}
# if we got here, we don't know how to handle the retrieval type
$self->throw("retrieval type " . $self->retrieval_type .
" unsupported\n");
}
=head2 url_base_address
Title : url_base_address
Usage : my $address = $self->url_base_address or
$self->url_base_address($address)
Function: Get/Set the base URL for the Web Database
Returns : Base URL for the Web Database
Args : $address - URL for the WebDatabase
=cut
sub url_base_address {
my $self = shift;
my $d = $self->{'_baseaddress'};
$self->{'_baseaddress'} = shift if @_;
$d;
}
=head2 proxy
Title : proxy
Usage : $httpproxy = $db->proxy('http') or
$db->proxy(['http','ftp'], 'http://myproxy' )
Function: Get/Set a proxy for use of proxy
Returns : a string indicating the proxy
Args : $protocol : an array ref of the protocol(s) to set/get
$proxyurl : url of the proxy to use for the specified protocol
$username : username (if proxy requires authentication)
$password : password (if proxy requires authentication)
=cut
sub proxy {
my ($self,$protocol,$proxy,$username,$password) = @_;
return if ( !defined $self->ua || !defined $protocol
|| !defined $proxy );
$self->authentication($username, $password)
if ($username && $password);
return $self->ua->proxy($protocol,$proxy);
}
=head2 authentication
Title : authentication
Usage : $db->authentication($user,$pass)
Function: Get/Set authentication credentials
Returns : Array of user/pass
Args : Array or user/pass
=cut
sub authentication{
my ($self,$u,$p) = @_;
if( defined $u && defined $p ) {
$self->{'_authentication'} = [ $u,$p];
}
return @{$self->{'_authentication'}};
}
=head2 retrieval_type
Title : retrieval_type
Usage : $self->retrieval_type($type);
my $type = $self->retrieval_type
Function: Get/Set a proxy for retrieval_type (pipeline, io_string or tempfile)
Returns : string representing retrieval type
Args : $value - the value to store
This setting affects how the data stream from the remote web server is
processed and passed to the Bio::SeqIO layer. Three types of retrieval
types are currently allowed:
pipeline Perform a fork in an attempt to begin streaming
while the data is still downloading from the remote
server. Disk, memory and speed efficient, but will
not work on Windows or MacOS 9 platforms.
io_string Store downloaded database entry(s) in memory. Can be
problematic for batch downloads because entire set
of entries must fit in memory. Alll entries must be
downloaded before processing can begin.
tempfile Store downloaded database entry(s) in a temporary file.
All entries must be downloaded before processing can
begin.
The default is pipeline, with automatic fallback to io_string if
pipelining is not available.
=cut
sub retrieval_type {
my ($self, $value) = @_;
if( defined $value ) {
$value = lc $value;
if( ! $RETRIEVAL_TYPES{$value} ) {
$self->warn("invalid retrieval type $value must be one of (" .
join(",", keys %RETRIEVAL_TYPES), ")");
$value = $DEFAULT_RETRIEVAL_TYPE;
}
$self->{'_retrieval_type'} = $value;
}
return $self->{'_retrieval_type'};
}
=head2 url_params
Title : url_params
Usage : my $params = $self->url_params or
$self->url_params($params)
Function: Get/Set the URL parameters for the Web Database
Returns : url parameters for Web Database
Args : $params - parameters to be appended to the URL for the WebDatabase
=cut
sub url_params {
my ($self, $value) = @_;
if( defined $value ) {
$self->{'_urlparams'} = $value;
}
}
=head2 ua
Title : ua
Usage : my $ua = $self->ua or
$self->ua($ua)
Function: Get/Set a LWP::UserAgent for use
Returns : reference to LWP::UserAgent Object
Args : $ua - must be a LWP::UserAgent
=cut
sub ua {
my ($self, $ua) = @_;
if( defined $ua && $ua->isa("LWP::UserAgent") ) {
$self->{'_ua'} = $ua;
}
return $self->{'_ua'};
}
=head2 postprocess_data
Title : postprocess_data
Usage : $self->postprocess_data ( 'type' => 'string',
'location' => \$datastr);
Function: process downloaded data before loading into a Bio::SeqIO
Returns : void
Args : hash with two keys - 'type' can be 'string' or 'file'
- 'location' either file location or string
reference containing data
=cut
sub postprocess_data {
my ( $self, %args) = @_;
return;
}
# private methods
sub _request {
my ($self, $url,$tmpfile) = @_;
my ($resp);
if( defined $tmpfile && $tmpfile ne '' ) {
$resp = $self->ua->request($url, $tmpfile);
} else {
$resp = $self->ua->request($url);
}
if( $resp->is_error ) {
$self->throw("WebDBSeqI Request Error:\n".$resp->as_string);
}
return $resp;
}
#mod_perl-safe replacement for the open(BLEH,'-|') call. if running
#under mod_perl, detects it and closes the child's STDIN and STDOUT
#handles
sub _open_pipe {
my ($self) = @_;
# is mod_perl running? Which API?
my $mp = $self->mod_perl_api;
if($mp and ! our $loaded_apache_sp) {
my $load_api = ($mp == 1) ? 'use Apache::SubProcess': 'use Apache2::SubProcess';
eval $load_api;
$@ and $self->throw("$@\n$load_api module required for running under mod_perl");
$loaded_apache_sp = 1;
}
my $pipe = IO::Pipe->new();
local $SIG{CHLD} = 'IGNORE';
defined(my $pid = fork)
or $self->throw("Couldn't fork: $!");
unless($pid) {
#CHILD
$pipe->writer();
#if we're running under mod_perl, clean up some things after this fork
if ($ENV{MOD_PERL} and my $r = eval{Apache->request} ) {
$r->cleanup_for_exec;
#don't read or write the mod_perl parent's tied filehandles
close STDIN; close STDOUT;
setsid() or $self->throw('Could not detach from parent');
}
} else {
#PARENT
$pipe->reader();
}
return ( $pid, $pipe );
}
# send web request to specified filehandle, or stdout, for streaming purposes
sub _stream_request {
my $self = shift;
my $request = shift;
my $dest_fh = shift || \*STDOUT;
# fork so as to pipe output of fetch process through to
# postprocess_data method call.
my ($child,$fetch) = $self->_open_pipe();
if ($child) {
#PARENT
local ($/) = "//\n"; # assume genbank/swiss format
$| = 1;
my $records = 0;
while (my $record = <$fetch>) {
$records++;
$self->postprocess_data('type' => 'string',
'location' => \$record);
print $dest_fh $record;
}
$/ = "\n"; # reset to be safe;
close $dest_fh; #must explicitly close here, because the hard
#exits don't cloes them for us
}
else {
#CHILD
$| = 1;
my $resp = $self->ua->request($request,
sub { print $fetch $_[0] }
);
if( $resp->is_error ) {
$self->throw("WebDBSeqI Request Error:\n".$resp->as_string);
}
close $fetch; #must explicitly close here, because the hard exists
#don't close them for us
POSIX::_exit(0);
}
}
sub io {
my ($self,$io) = @_;
if(defined($io) || (! exists($self->{'_io'}))) {
$io = Bio::Root::IO->new() unless $io;
$self->{'_io'} = $io;
}
return $self->{'_io'};
}
=head2 delay
Title : delay
Usage : $secs = $self->delay([$secs])
Function: get/set number of seconds to delay between fetches
Returns : number of seconds to delay
Args : new value
NOTE: the default is to use the value specified by delay_policy().
This can be overridden by calling this method, or by passing the
-delay argument to new().
=cut
sub delay {
my $self = shift;
my $d = $self->{'_delay'};
$self->{'_delay'} = shift if @_;
$d;
}
=head2 delay_policy
Title : delay_policy
Usage : $secs = $self->delay_policy
Function: return number of seconds to delay between calls to remote db
Returns : number of seconds to delay
Args : none
NOTE: The default delay policy is 0s. Override in subclasses to
implement delays. The timer has only second resolution, so the delay
will actually be +/- 1s.
=cut
sub delay_policy {
my $self = shift;
return 0;
}
=head2 _sleep
Title : _sleep
Usage : $self->_sleep
Function: sleep for a number of seconds indicated by the delay policy
Returns : none
Args : none
NOTE: This method keeps track of the last time it was called and only
imposes a sleep if it was called more recently than the delay_policy()
allows.
=cut
sub _sleep {
my $self = shift;
my $last_invocation = $LAST_INVOCATION_TIME;
if (time - $LAST_INVOCATION_TIME < $self->delay) {
my $delay = $self->delay - (time - $LAST_INVOCATION_TIME);
warn "sleeping for $delay seconds\n" if $self->verbose > 0;
sleep $delay;
}
$LAST_INVOCATION_TIME = time;
}
=head2 mod_perl_api
Title : mod_perl_api
Usage : $version = self->mod_perl_api
Function: Returns API version of mod_perl being used based on set env. variables
Returns : mod_perl API version; if mod_perl isn't loaded, returns 0
Args : none
=cut
sub mod_perl_api {
my $self = shift;
my $v = $ENV{MOD_PERL} ?
( exists $ENV{MOD_PERL_API_VERSION} && $ENV{MOD_PERL_API_VERSION} >= 2 ) ?
2 :
1
: 0;
return $v;
}
1;