PfHRP3exon2_README.txt

All Pfhrp2 exon 2 and Pfhrp2 exon 3 nucleotide sequences were exported from CodonCode Aligner (CodonCode Corporation) and individually pasted into the ExPASy Translate tool (Swiss Institute of Bioinformatics Resource Portal). Both forward and reverse DNA strands were translated using the standard NCBI genetic code. The six reading frames of the amino acid sequence produced were examined. 
For each nucleotide sequence, the amino acid sequence presenting the fewest number of stop codons was selected for further analysis. If two or more of the reading frames appeared to produce sequences with an equally minimal number of stop codons, the reading frame that produced a sequence exhibiting the previously recognized pattern in prior sequences was selected for further analysis. While most of the sequences had a clear, single best translation, 11 of the sequences required further editing. In these 11 sequences, the sequence portion before or after the stop codon which exhibited a pattern similar to prior sequences was used in analysis, while the portion of the sequence preceding or following the stop codon, which did not exhibit the recognized pattern, was discarded. Nucleotide sequence input into the ExPASy Translate Tool (Swiss Institute of Bioinformatics Resource Portal) was repeated and verified for accuracy of amino acid sequences. The verified sequences were compiled.