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metadata.yaml
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'@context': https://raw.githubusercontent.com/dandi/schema/master/releases/0.6.4/context.json
about: []
access:
- schemaKey: AccessRequirements
status: dandi:OpenAccess
assetsSummary:
approach:
- name: microscopy approach; cell population imaging
schemaKey: ApproachType
dataStandard:
- identifier: RRID:SCR_015242
name: Neurodata Without Borders (NWB)
schemaKey: StandardsType
measurementTechnique:
- name: analytical technique
schemaKey: MeasurementTechniqueType
- name: two-photon microscopy technique
schemaKey: MeasurementTechniqueType
- name: surgical technique
schemaKey: MeasurementTechniqueType
numberOfBytes: 15338089927
numberOfFiles: 2
numberOfSubjects: 1
schemaKey: AssetsSummary
species:
- identifier: http://purl.obolibrary.org/obo/NCBITaxon_10090
name: Mus musculus - House mouse
schemaKey: SpeciesType
variableMeasured:
- TwoPhotonSeries
- ImagingPlane
- ProcessingModule
- PlaneSegmentation
- OpticalChannel
citation: Scherrer, Joseph R.; Lynch, Galen F.; Zhang, Jie J.; Fee, Michale S. (2024)
An optical design enabling lightweight and large field-of-view head-mounted microscopes
(Version draft) [Data set]. DANDI archive. https://dandiarchive.org/dandiset/000691/draft
contributor:
- affiliation: []
email: fee@mit.edu
identifier: 0000-0003-0424-3483
includeInCitation: true
name: Scherrer, Joseph R.
roleName:
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- dcite:ContactPerson
schemaKey: Person
- affiliation: []
includeInCitation: true
name: Lynch, Galen F.
roleName:
- dcite:Author
schemaKey: Person
- affiliation: []
includeInCitation: true
name: Zhang, Jie J.
roleName:
- dcite:Author
schemaKey: Person
- affiliation: []
email: fee@mit.edu
identifier: 0000-0001-7539-1745
includeInCitation: true
name: Fee, Michale S.
roleName:
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schemaKey: Person
- awardNumber: 542977ASPI
contactPoint: []
identifier: https://ror.org/01cmst727
includeInCitation: false
name: Simons Foundation
roleName:
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schemaKey: Organization
- awardNumber: R21 EY028381-01
contactPoint: []
identifier: https://ror.org/01cwqze88
includeInCitation: false
name: National Institutes of Health
roleName:
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schemaKey: Organization
- contactPoint: []
includeInCitation: false
name: McKnight Foundation
roleName:
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schemaKey: Organization
- contactPoint: []
includeInCitation: false
name: Harold and Ruth Newman Family Hertz Graduate Fellowship
roleName:
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schemaKey: Organization
dateCreated: '2023-10-30T15:17:41.803046+00:00'
description: "Here we present a fluorescence microscope light path that enables imaging,
during free behavior, of thousands of neurons in mice and hundreds of neurons in
juvenile songbirds. The light path eliminates traditional illumination optics, allowing
for head-mounted microscopes that have both a lower weight and a larger field of
view (FOV) than previously possible. Using this light path, we designed two microscopes:
one optimized for FOV (~4 mm FOV; 1.4 g), and the other optimized for weight (1.0
mm FOV; 1.0 g). \nThis dataset includes the calcium imaging data from our microscope
optimized for weight. For this experiment, we stereotactically located a region
of the mouse brain at coordinates associatied with primary visual and somatosensory
regions, performed approximately 20 viral injections of GCaMP7f across a 4-mm-diameter
region of cortex, and then implanted a 4-mm-diameter glass window in the skull.
After letting each mouse recover, we installed our head-mounted microscope and allowed
it to freely explore a circular maze while recording calcium activity for several
minutes."
ethicsApproval: []
id: DANDI:000691/draft
identifier: DANDI:000691
keywords: []
license:
- spdx:CC-BY-4.0
manifestLocation:
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name: An optical design enabling lightweight and large field-of-view head-mounted
microscopes
protocol: []
relatedResource:
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microscopes
name: An optical design enabling lightweight and large field-of-view head-mounted
microscopes
relation: dcite:IsDescribedBy
repository: Nature Methods
resourceType: dcite:JournalArticle
schemaKey: Resource
url: https://doi.org/10.1038/s41592-023-01806-1
repository: https://dandiarchive.org
schemaKey: Dandiset
schemaVersion: 0.6.4
studyTarget: []
url: https://dandiarchive.org/dandiset/000691/draft
version: draft
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