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<title>Antartic Microbes</title>
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<h1><a href="index.html">Antartic Microbes</a></h1>
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<h3>Diversidade de bactérias anaeróbias cultiváveis associadas à rizosfera da planta vascular <i>Deschampsia Antarctica</i> presente na Ilha Rei George, Antártica. </h3>
<p class="author">Rafael José Marques Peixoto</p>
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<p class="pretext">Abstract</p>
<span class="captalized-text">T</span>he Antarctic continent presents a peculiar geographical isolation and represents an intact reservoir of life forms. The rhizosphere soil from vascular plants shows an anaerobic microbial predominance never founde, raising the anaerobic bacteriology to an important research focus at this site. Here we analyzed different methods of treatments in order to verify better strategies for anaerobic bacteria isolation and their diversity, from the rhizosphere (<i>D. Antarctica</i>) soil collected in Admiralty Bay, Antarctic. Some authors estimate that only less than 1% of all microbial species found in natural environments is easily isolated and cultivated in artificial culture media. Therefore, one of the main challenges in studies of anaerobes is to find isolation methods that achieve remaining unculturable bacteria. Comparisons between incubation in high-column thioglycollate medium with or without heat shock in 27 different culture conditions revealed that the efficiency of these pre-treatments is extremely dependent on the physical-chemical parameters used in culture. However, commonly used procedures under pH 7 and 0.5% NaCl medium concentration indicate the combined use of high-column thioglycollate medium pre-incubation with heat shock seems to increase frequency and abundance of anaerobic bacteria isolation in a quantitative aspect. In a qualitative aspect we found four principal phylum: Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria; 23 candidate to new species. We suggest that varying sample treatments and isolation culture conditions may be an useful way to improve access to diversity of anaerobic bacteria in environmental samples.
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<p>Bioremediation</p>
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<p>Bacteria</p>
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<p>Rhizosphere</p>
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<p>Instituto Oceanográfico</p>
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