diff --git a/runtime/js/99_main.js b/runtime/js/99_main.js
index 8f53cffc42df92..9134ac48a15d95 100644
--- a/runtime/js/99_main.js
+++ b/runtime/js/99_main.js
@@ -654,6 +654,7 @@ function bootstrapMainRuntime(runtimeOptions, warmup = false) {
     if (location_ == null) {
       mainRuntimeGlobalProperties.location = {
         writable: true,
+        configurable: true,
       };
     } else {
       location.setLocationHref(location_);
diff --git a/tests/specs/run/location/__test__.jsonc b/tests/specs/run/location/__test__.jsonc
new file mode 100644
index 00000000000000..551463d594d8dd
--- /dev/null
+++ b/tests/specs/run/location/__test__.jsonc
@@ -0,0 +1,8 @@
+{
+  "tests": {
+    "location_object_define_property": {
+      "args": "run location.js",
+      "output": "location.out"
+    }
+  }
+}
diff --git a/tests/specs/run/location/location.js b/tests/specs/run/location/location.js
new file mode 100644
index 00000000000000..8562a39957d084
--- /dev/null
+++ b/tests/specs/run/location/location.js
@@ -0,0 +1,24 @@
+let _location = undefined;
+
+console.log(globalThis.location);
+
+Object.defineProperty(globalThis, "location", {
+  get() {
+    return _location;
+  },
+  set(v) {
+    _location = v;
+  },
+  configurable: true,
+});
+
+console.log(globalThis.location);
+
+globalThis.location = "https://deno.com";
+
+console.log(_location);
+console.log(location);
+
+delete globalThis["location"];
+
+console.log(globalThis.location);
diff --git a/tests/specs/run/location/location.out b/tests/specs/run/location/location.out
new file mode 100644
index 00000000000000..bcb3ff67b91dbf
--- /dev/null
+++ b/tests/specs/run/location/location.out
@@ -0,0 +1,5 @@
+undefined
+undefined
+https://deno.com
+https://deno.com
+undefined