diff --git a/src/libraries/System.Net.Quic/src/System/Net/Quic/Internal/MsQuicApi.cs b/src/libraries/System.Net.Quic/src/System/Net/Quic/Internal/MsQuicApi.cs
index e07a99b13f7c70..2e5f1e6d546df4 100644
--- a/src/libraries/System.Net.Quic/src/System/Net/Quic/Internal/MsQuicApi.cs
+++ b/src/libraries/System.Net.Quic/src/System/Net/Quic/Internal/MsQuicApi.cs
@@ -65,6 +65,8 @@ private MsQuicApi(QUIC_API_TABLE* apiTable)
     internal static bool Tls13ClientMayBeDisabled { get; }
 
 #pragma warning disable CA1810 // Initialize all static fields in 'MsQuicApi' when those fields are declared and remove the explicit static constructor
+    [UnconditionalSuppressMessage("SingleFile", "IL3000: Avoid accessing Assembly file path when publishing as a single file",
+        Justification = "The code handles the Assembly.Location being null/empty by falling back to AppContext.BaseDirectory")]
     static MsQuicApi()
     {
         bool loaded = false;