diff --git a/src/tests/issues.targets b/src/tests/issues.targets
index 221ed02229eb4..05b995cd0d6b0 100644
--- a/src/tests/issues.targets
+++ b/src/tests/issues.targets
@@ -72,9 +72,6 @@
         <ExcludeList Include="$(XunitTestBinBase)/JIT/Regression/JitBlue/Runtime_82535/Runtime_82535/*">
           <Issue>https://github.com/dotnet/runtime/issues/89585</Issue>
         </ExcludeList>
-        <ExcludeList Include="$(XunitTestBinBase)/readytorun/GenericCycleDetection/Depth3Test/*">
-          <Issue>https://github.com/dotnet/runtime/issues/88586</Issue>
-        </ExcludeList>
     </ItemGroup>
 
     <!-- All Unix targets on all runtimes -->
diff --git a/src/tests/readytorun/GenericCycleDetection/Depth3Test.cs b/src/tests/readytorun/GenericCycleDetection/Depth3Test.cs
index 2edaef57d2681..565079d5d269c 100644
--- a/src/tests/readytorun/GenericCycleDetection/Depth3Test.cs
+++ b/src/tests/readytorun/GenericCycleDetection/Depth3Test.cs
@@ -23,7 +23,6 @@ public static long TypeNestedFactorial(int count)
             if (result < count) result = Depth1<Depth2<T>>.TypeNestedFactorial(count - 1);
             if (result < count) result = Depth1<Depth3<T>>.TypeNestedFactorial(count - 1);
             if (result < count) result = Depth1<Depth4<T>>.TypeNestedFactorial(count - 1);
-            if (result < count) result = Depth1<Depth5<T>>.TypeNestedFactorial(count - 1);
             return count * result;
         }
     }
@@ -31,7 +30,6 @@ public static long TypeNestedFactorial(int count)
     private struct Depth2<T> {}
     private struct Depth3<T> {}
     private struct Depth4<T> {}
-    private struct Depth5<T> {}
     
     [Fact]
     public static int DepthTest()