diff --git a/eng/native/configurecompiler.cmake b/eng/native/configurecompiler.cmake
index 2b2a6c0e0a349..0f5aaa57820f7 100644
--- a/eng/native/configurecompiler.cmake
+++ b/eng/native/configurecompiler.cmake
@@ -68,9 +68,6 @@ if (MSVC)
   add_compile_options($<$<COMPILE_LANGUAGE:CXX>:$<TARGET_PROPERTY:CLR_EH_OPTION>>)
   add_link_options($<$<BOOL:$<TARGET_PROPERTY:CLR_CONTROL_FLOW_GUARD>>:/guard:cf>)
 
-  # Load all imported DLLs from the System32 directory.
-  add_linker_flag(/DEPENDENTLOADFLAG:0x800)
-
   # Linker flags
   #
   set (WINDOWS_SUBSYSTEM_VERSION 6.01)
diff --git a/eng/native/ijw/IJW.cmake b/eng/native/ijw/IJW.cmake
index 5782babe75549..71d40fc282385 100644
--- a/eng/native/ijw/IJW.cmake
+++ b/eng/native/ijw/IJW.cmake
@@ -64,10 +64,6 @@ if (CLR_CMAKE_HOST_WIN32)
   remove_ijw_incompatible_options("${dirCompileOptions}" dirCompileOptions)
   set_directory_properties(PROPERTIES COMPILE_OPTIONS "${dirCompileOptions}")
 
-  # IJW tests needs to load DLLs from somewhere other than System32
-  string(REPLACE "/DEPENDENTLOADFLAG:0x800" "" CMAKE_EXE_LINKER_FLAGS "${CMAKE_EXE_LINKER_FLAGS}")
-  string(REPLACE "/DEPENDENTLOADFLAG:0x800" "" CMAKE_SHARED_LINKER_FLAGS "${CMAKE_SHARED_LINKER_FLAGS}")
-
   set(CLR_SDK_REF_PACK_OUTPUT "")
   set(CLR_SDK_REF_PACK_DISCOVERY_ERROR "")
   set(CLR_SDK_REF_PACK_DISCOVERY_RESULT 0)
diff --git a/src/coreclr/build-runtime.cmd b/src/coreclr/build-runtime.cmd
index 71ab99aaccf24..b52037b80e438 100644
--- a/src/coreclr/build-runtime.cmd
+++ b/src/coreclr/build-runtime.cmd
@@ -433,10 +433,6 @@ if %__BuildNative% EQU 1 (
         goto ExitWithCode
     )
 
-    @REM Temporarily disabling PGO until updated files with new linker flag are available.
-    @REM https://github.com/dotnet/runtime/pull/89311
-    GOTO :SkipNativeBuild
-
     if %__EnforcePgo% EQU 1 (
         set PgoCheckCmd="!PYTHON!" "!__ProjectDir!\scripts\pgocheck.py" "!__BinDir!\coreclr.dll" "!__BinDir!\clrjit.dll"
         echo !PgoCheckCmd!
diff --git a/src/coreclr/pgosupport.cmake b/src/coreclr/pgosupport.cmake
index 6d7edfb62ca70..34a42781ab2c8 100644
--- a/src/coreclr/pgosupport.cmake
+++ b/src/coreclr/pgosupport.cmake
@@ -59,11 +59,9 @@ function(add_pgo TargetName)
             message("Cannot use PGO optimization built with Ninja from MSBuild. Re-run build with Ninja to apply PGO information")
         else(NOT EXISTS ${ProfilePath})
             if(CLR_CMAKE_HOST_WIN32)
-                # Temporarily disabling PGO until updated files with new linker flag are available.
-                # https://github.com/dotnet/runtime/pull/89311
-                # set_property(TARGET ${TargetName} APPEND_STRING PROPERTY LINK_FLAGS_RELEASE        " /LTCG /USEPROFILE:PGD=\"${ProfilePath}\"")
-                # set_property(TARGET ${TargetName} APPEND_STRING PROPERTY LINK_FLAGS_RELWITHDEBINFO " /LTCG /USEPROFILE:PGD=\"${ProfilePath}\"")
-                # add_compile_definitions(WITH_NATIVE_PGO)
+                set_property(TARGET ${TargetName} APPEND_STRING PROPERTY LINK_FLAGS_RELEASE        " /LTCG /USEPROFILE:PGD=\"${ProfilePath}\"")
+                set_property(TARGET ${TargetName} APPEND_STRING PROPERTY LINK_FLAGS_RELWITHDEBINFO " /LTCG /USEPROFILE:PGD=\"${ProfilePath}\"")
+                add_compile_definitions(WITH_NATIVE_PGO)
             else(CLR_CMAKE_HOST_WIN32)
                 if(UPPERCASE_CMAKE_BUILD_TYPE STREQUAL RELEASE OR UPPERCASE_CMAKE_BUILD_TYPE STREQUAL RELWITHDEBINFO)
                     if((CMAKE_CXX_COMPILER_ID MATCHES "Clang") AND (NOT CMAKE_CXX_COMPILER_VERSION VERSION_LESS 16))