diff --git a/eng/testing/linker/project.csproj.template b/eng/testing/linker/project.csproj.template
index 41466e8d4492af..d31c8df9c9271e 100644
--- a/eng/testing/linker/project.csproj.template
+++ b/eng/testing/linker/project.csproj.template
@@ -75,6 +75,12 @@
     {AdditionalProjectReferences}
   </ItemGroup>
 
+  <Target Name="RemoveInvariantGlobalization" BeforeTargets="_SetWasmBuildNativeDefaults" Condition="'$(TargetArchitecture)' == 'wasm'">
+    <ItemGroup>
+      <_BoolPropertiesThatTriggerRelinking Remove="InvariantGlobalization" />
+    </ItemGroup>
+  </Target>
+
   <Target Name="LocateNativeCompiler"
           Condition="'$(PublishAot)' == 'true' and '$(_hostOS)' != 'win'"
           BeforeTargets="SetupOSSpecificProps">
diff --git a/src/mono/browser/build/BrowserWasmApp.targets b/src/mono/browser/build/BrowserWasmApp.targets
index 6174769ef02ea4..aa231c45d8b7cc 100644
--- a/src/mono/browser/build/BrowserWasmApp.targets
+++ b/src/mono/browser/build/BrowserWasmApp.targets
@@ -64,7 +64,6 @@
 
     <_BoolPropertiesThatTriggerRelinking Include="WasmEnableSIMD" DefaultValueInRuntimePack="true" />
     <_BoolPropertiesThatTriggerRelinking Include="WasmEnableExceptionHandling" DefaultValueInRuntimePack="true" />
-    <_BoolPropertiesThatTriggerRelinking Include="WasmNativeStrip" DefaultValueInRuntimePack="true" />
   </ItemGroup>
 
   <Target Name="_GetWasmGenerateAppBundleDependencies">
diff --git a/src/mono/wasi/build/WasiApp.targets b/src/mono/wasi/build/WasiApp.targets
index b31c573ea349b5..b229d76dd71ff9 100644
--- a/src/mono/wasi/build/WasiApp.targets
+++ b/src/mono/wasi/build/WasiApp.targets
@@ -39,7 +39,6 @@
   <ItemGroup>
     <_BoolPropertiesThatTriggerRelinking Include="WasmEnableSIMD" DefaultValueInRuntimePack="false" />
     <!--<_BoolPropertiesThatTriggerRelinking Include="WasmEnableExceptionHandling" DefaultValueInRuntimePack="true" />-->
-    <_BoolPropertiesThatTriggerRelinking Include="WasmNativeStrip" DefaultValueInRuntimePack="true" />
   </ItemGroup>
 
   <Target Name="_GetWasiGenerateAppBundleDependencies">
diff --git a/src/mono/wasm/Wasm.Build.Tests/NativeBuildTests.cs b/src/mono/wasm/Wasm.Build.Tests/NativeBuildTests.cs
index ac8b567761f36a..f7c7483a949d3f 100644
--- a/src/mono/wasm/Wasm.Build.Tests/NativeBuildTests.cs
+++ b/src/mono/wasm/Wasm.Build.Tests/NativeBuildTests.cs
@@ -93,5 +93,24 @@ public void IntermediateBitcodeToObjectFilesAreNotLLVMIR(BuildArgs buildArgs, st
                                             + " It might fail if it was incorrectly compiled to a bitcode file, instead of wasm.");
         }
 
+        [Theory]
+        [BuildAndRun(host: RunHost.None, aot: true)]
+        public void NativeBuildIsRequired(BuildArgs buildArgs, string id)
+        {
+            string projectName = $"native_build_{buildArgs.Config}_{buildArgs.AOT}";
+
+            buildArgs = buildArgs with { ProjectName = projectName, ExtraBuildArgs = "-p:WasmBuildNative=false -p:WasmSingleFileBundle=true" };
+            buildArgs = ExpandBuildArgs(buildArgs);
+
+            (_, string output) = BuildProject(
+                                    buildArgs,
+                                    id: id,
+                                    new BuildProjectOptions(
+                                        InitProject: () => File.WriteAllText(Path.Combine(_projectDir!, "Program.cs"), s_mainReturns42),
+                                        DotnetWasmFromRuntimePack: false,
+                                        ExpectSuccess: false));
+
+            Assert.Contains("WasmBuildNative is required", output);
+        }
     }
 }
diff --git a/src/mono/wasm/build/WasmApp.Common.targets b/src/mono/wasm/build/WasmApp.Common.targets
index f0c0d4be6946ab..5393cfb84ba794 100644
--- a/src/mono/wasm/build/WasmApp.Common.targets
+++ b/src/mono/wasm/build/WasmApp.Common.targets
@@ -111,6 +111,13 @@
   <UsingTask TaskName="MonoTargetsTasks.MarshalingPInvokeScanner" AssemblyFile="$(MonoTargetsTasksAssemblyPath)" />
   <UsingTask TaskName="EmitBundleObjectFiles" AssemblyFile="$(MonoTargetsTasksAssemblyPath)" />
 
+  <ItemGroup>
+      <_BoolPropertiesThatTriggerRelinking Include="InvariantTimezone" DefaultValueInRuntimePack="false" />
+      <_BoolPropertiesThatTriggerRelinking Include="InvariantGlobalization" DefaultValueInRuntimePack="false" />
+      <_BoolPropertiesThatTriggerRelinking Include="WasmNativeStrip" DefaultValueInRuntimePack="true" />
+      <_BoolPropertiesThatTriggerRelinking Include="WasmSingleFileBundle" DefaultValueInRuntimePack="false" />
+  </ItemGroup>
+
   <PropertyGroup>
     <PrepareInputsForWasmBuildDependsOn>
       $(PrepareInputsForWasmBuildDependsOn);
@@ -494,14 +501,18 @@
            Text="$(_ToolchainMissingErrorMessage) SDK is required for AOT'ing assemblies." />
 
     <ItemGroup>
-      <_BoolPropertiesThatTriggerRelinking Include="InvariantTimezone" DefaultValueInRuntimePack="false" />
-      <_BoolPropertiesThatTriggerRelinking Include="InvariantGlobalization" DefaultValueInRuntimePack="false" />
+      <_ChangedBoolPropertiesThatTriggerRelinking Include="%(_BoolPropertiesThatTriggerRelinking.Identity)" Condition="'$(%(_BoolPropertiesThatTriggerRelinking.Identity))' != '' and 
+                                                                                                            '$(%(_BoolPropertiesThatTriggerRelinking.Identity))' != '%(_BoolPropertiesThatTriggerRelinking.DefaultValueInRuntimePack)'" />
     </ItemGroup>
+    <PropertyGroup>
+      <_WasmBuildNativeRequired Condition="@(_ChangedBoolPropertiesThatTriggerRelinking->Count()) > 0">true</_WasmBuildNativeRequired>
+    </PropertyGroup>
+
+    <Error Condition="'$(WasmBuildNative)' == 'false' and '$(_WasmBuildNativeRequired)' == 'true'" 
+           Text="WasmBuildNative is required because %(_ChangedBoolPropertiesThatTriggerRelinking.Identity)=$(%(_ChangedBoolPropertiesThatTriggerRelinking.Identity)), but WasmBuildNative is already set to 'false'." />
 
     <PropertyGroup>
-      <WasmBuildNative Condition="'$(WasmBuildNative)' == '' and
-                          '$(%(_BoolPropertiesThatTriggerRelinking.Identity))' != '' and
-                          '$(%(_BoolPropertiesThatTriggerRelinking.Identity))' != '%(_BoolPropertiesThatTriggerRelinking.DefaultValueInRuntimePack)'">true</WasmBuildNative>
+      <WasmBuildNative Condition="'$(WasmBuildNative)' == '' and '$(_WasmBuildNativeRequired)' == 'true'">true</WasmBuildNative>
     </PropertyGroup>
 
     <!-- When Building -->
@@ -510,7 +521,6 @@
       <WasmBuildNative Condition="'$(RunAOTCompilation)' == 'true' and '$(RunAOTCompilationAfterBuild)' == 'true'">true</WasmBuildNative>
 
       <WasmBuildNative Condition="'$(WasmBuildNative)' == '' and @(NativeFileReference->Count()) > 0" >true</WasmBuildNative>
-      <WasmBuildNative Condition="'$(WasmBuildNative)' == '' and '$(WasmSingleFileBundle)' == 'true'">true</WasmBuildNative>
     </PropertyGroup>
 
     <!-- When Publishing -->
@@ -518,7 +528,6 @@
       <!-- AOT==true overrides WasmBuildNative -->
       <WasmBuildNative Condition="'$(RunAOTCompilation)' == 'true'">true</WasmBuildNative>
       <WasmBuildNative Condition="'$(WasmBuildNative)' == '' and @(NativeFileReference->Count()) > 0" >true</WasmBuildNative>
-      <WasmBuildNative Condition="'$(WasmBuildNative)' == '' and '$(WasmSingleFileBundle)' == 'true'">true</WasmBuildNative>
 
       <!-- not aot, not trimmed app, no reason to relink -->
       <WasmBuildNative Condition="'$(WasmBuildNative)' == '' and '$(PublishTrimmed)' != 'true'">false</WasmBuildNative>