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Reliably detect M.bovis when it's at low prevalence in the sample #17

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ellisrichardj opened this issue Apr 3, 2020 · 3 comments
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@ellisrichardj
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Need to filter kraken/bracken output so that presence of M.bovis in the sample is recognised and flagged. This should be added to the output csv file

@ellisrichardj ellisrichardj self-assigned this Apr 3, 2020
@ellisrichardj ellisrichardj added the enhancement New feature or request label Apr 3, 2020
@ellisrichardj ellisrichardj changed the title Reliably detect M.bovis when its at low prevalence in the sample Reliably detect M.bovis when it's at low prevalence in the sample Apr 18, 2020
@ellisrichardj
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M. bovis is classified as 'S1' level in kraken output (M. tuberculosis var. bovis) so bracken needs to filter on this level

@ellisrichardj
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Use grep to parse bracken output for M.bovis presence. However, if not present grep gives and exit code and process fails.

@ellisrichardj
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Need to look though existing data and determine threshold / cut-off abundance for positive/negative

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