diff --git a/tox.ini b/tox.ini
index bf7909a66f1c..ab1153917d2e 100644
--- a/tox.ini
+++ b/tox.ini
@@ -46,6 +46,8 @@ setenv =
     first_startup: GALAXY_CONFIG_DATABASE_AUTO_MIGRATE=true
     mulled: marker=external_dependency_management
     mulled,unit: GALAXY_VIRTUAL_ENV={envdir}
+    mypy,test_galaxy_packages,test_galaxy_packages_for_pulsar: PIP_INDEX_URL=https://wheels.galaxyproject.org/simple
+    mypy,test_galaxy_packages,test_galaxy_packages_for_pulsar: PIP_EXTRA_INDEX_URL=https://pypi.python.org/simple
     unit: GALAXY_ENABLE_BETA_COMPRESSED_GENBANK_SNIFFING=1
     unit: marker=not external_dependency_management
 deps =