diff --git a/scene/2d/tile_map.cpp b/scene/2d/tile_map.cpp
index 1ea342c3f441..fe19042e4544 100644
--- a/scene/2d/tile_map.cpp
+++ b/scene/2d/tile_map.cpp
@@ -685,37 +685,43 @@ void TileMapLayer::_physics_update() {
 }
 
 void TileMapLayer::_physics_notify_tilemap_change(TileMapLayer::DirtyFlags p_what) {
-	Transform2D gl_transform = tile_map_node->get_global_transform();
+	if (!tile_map_node->is_inside_tree()) {
+		return;
+	}
+
+	PhysicsServer2D *ps = PhysicsServer2D::get_singleton();
+
 	bool in_editor = false;
 #ifdef TOOLS_ENABLED
 	in_editor = Engine::get_singleton()->is_editor_hint();
 #endif
 
-	if (p_what == DIRTY_FLAGS_TILE_MAP_XFORM) {
-		if (tile_map_node->is_inside_tree() && (!tile_map_node->is_collision_animatable() || in_editor)) {
-			for (KeyValue<Vector2i, CellData> &kv : tile_map) {
-				const CellData &cell_data = kv.value;
+	if (
+			(p_what == DIRTY_FLAGS_TILE_MAP_XFORM && (!tile_map_node->is_collision_animatable() || in_editor)) ||
+			(p_what == DIRTY_FLAGS_TILE_MAP_LOCAL_XFORM && (tile_map_node->is_collision_animatable() && !in_editor))) {
+		Transform2D gl_transform = tile_map_node->get_global_transform();
 
-				for (RID body : cell_data.bodies) {
-					if (body.is_valid()) {
-						Transform2D xform(0, tile_map_node->map_to_local(bodies_coords[body]));
-						xform = gl_transform * xform;
-						PhysicsServer2D::get_singleton()->body_set_state(body, PhysicsServer2D::BODY_STATE_TRANSFORM, xform);
-					}
+		for (KeyValue<Vector2i, CellData> &kv : tile_map) {
+			const CellData &cell_data = kv.value;
+
+			for (RID body : cell_data.bodies) {
+				if (body.is_valid()) {
+					Transform2D xform(0, tile_map_node->map_to_local(bodies_coords[body]));
+					xform = gl_transform * xform;
+					ps->body_set_state(body, PhysicsServer2D::BODY_STATE_TRANSFORM, xform);
 				}
 			}
 		}
-	} else if (p_what == DIRTY_FLAGS_TILE_MAP_LOCAL_XFORM) {
-		if (tile_map_node->is_inside_tree() && tile_map_node->is_collision_animatable() && !in_editor) {
-			for (KeyValue<Vector2i, CellData> &kv : tile_map) {
-				const CellData &cell_data = kv.value;
+	} else if (p_what == DIRTY_FLAGS_TILE_MAP_IN_TREE) {
+		// Changes in the tree may cause the space to change (e.g. when reparenting to a SubViewport)
+		RID space = tile_map_node->get_world_2d()->get_space();
 
-				for (RID body : cell_data.bodies) {
-					if (body.is_valid()) {
-						Transform2D xform(0, tile_map_node->map_to_local(bodies_coords[body]));
-						xform = gl_transform * xform;
-						PhysicsServer2D::get_singleton()->body_set_state(body, PhysicsServer2D::BODY_STATE_TRANSFORM, xform);
-					}
+		for (KeyValue<Vector2i, CellData> &kv : tile_map) {
+			const CellData &cell_data = kv.value;
+
+			for (RID body : cell_data.bodies) {
+				if (body.is_valid()) {
+					ps->body_set_space(body, space);
 				}
 			}
 		}