diff --git a/CHANGELOG.md b/CHANGELOG.md
index 91b3fac110bb5..20198502e6281 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -2,7 +2,7 @@
 
 ## New Features:
 
-No changes to highlight.
+- Adds a `root_path` parameter to `launch()` that allows running Gradio applications on subpaths (e.g. www.example.com/app) behind a proxy, by [@abidlabs](https://github.com/abidlabs) in [PR 4133](https://github.com/gradio-app/gradio/pull/4133)
 
 ## Bug Fixes:
 
diff --git a/gradio/blocks.py b/gradio/blocks.py
index e6c28a94b4b17..f890206882afe 100644
--- a/gradio/blocks.py
+++ b/gradio/blocks.py
@@ -93,7 +93,6 @@ def __init__(
         self._skip_init_processing = _skip_init_processing
         self._style = {}
         self.parent: BlockContext | None = None
-        self.root = ""
 
         if render:
             self.render()
@@ -717,6 +716,7 @@ def __init__(
 
         self.allowed_paths = []
         self.blocked_paths = []
+        self.root_path = ""
 
         if self.analytics_enabled:
             is_custom_theme = not any(
@@ -1353,7 +1353,6 @@ def get_config_file(self):
             "show_api": self.show_api,
             "is_colab": utils.colab_check(),
             "stylesheets": self.stylesheets,
-            "root": self.root,
             "theme": self.theme.name,
         }
 
@@ -1606,6 +1605,7 @@ def launch(
         file_directories: list[str] | None = None,
         allowed_paths: list[str] | None = None,
         blocked_paths: list[str] | None = None,
+        root_path: str = "",
         _frontend: bool = True,
     ) -> tuple[FastAPI, str, str]:
         """
@@ -1639,6 +1639,7 @@ def launch(
             file_directories: This parameter has been renamed to `allowed_paths`. It will be removed in a future version.
             allowed_paths: List of complete filepaths or parent directories that gradio is allowed to serve (in addition to the directory containing the gradio python file). Must be absolute paths. Warning: if you provide directories, any files in these directories or their subdirectories are accessible to all users of your app.
             blocked_paths: List of complete filepaths or parent directories that gradio is not allowed to serve (i.e. users of your app are not allowed to access). Must be absolute paths. Warning: takes precedence over `allowed_paths` and all other directories exposed by Gradio by default.
+            root_path: The root path (or "mount point") of the application, if it's not served from the root ("/") of the domain. Often used when the application is behind a reverse proxy that forwards requests to the application. For example, if the application is served at "https://example.com/myapp", the `root_path` should be set to "/myapp".
         Returns:
             app: FastAPI app object that is running the demo
             local_url: Locally accessible link to the demo
@@ -1678,6 +1679,7 @@ def reverse(text):
         self.width = width
         self.favicon_path = favicon_path
         self.ssl_verify = ssl_verify
+        self.root_path = root_path
 
         if enable_queue is not None:
             self.enable_queue = enable_queue
diff --git a/gradio/routes.py b/gradio/routes.py
index e275d5ae2c60b..49fcc6e282a4a 100644
--- a/gradio/routes.py
+++ b/gradio/routes.py
@@ -133,6 +133,7 @@ def configure_app(self, blocks: gradio.Blocks) -> None:
         self.cwd = os.getcwd()
         self.favicon_path = blocks.favicon_path
         self.tokens = {}
+        self.root_path = blocks.root_path
 
     def get_blocks(self) -> gradio.Blocks:
         if self.blocks is None:
@@ -222,15 +223,17 @@ def login(form_data: OAuth2PasswordRequestForm = Depends()):
         def main(request: fastapi.Request, user: str = Depends(get_current_user)):
             mimetypes.add_type("application/javascript", ".js")
             blocks = app.get_blocks()
+            root_path = request.scope.get("root_path", "")
 
             if app.auth is None or user is not None:
                 config = app.get_blocks().config
+                config["root"] = root_path
             else:
                 config = {
                     "auth_required": True,
                     "auth_message": blocks.auth_message,
                     "is_space": app.get_blocks().is_space,
-                    "root": app.get_blocks().root,
+                    "root": root_path,
                 }
 
             try:
@@ -261,8 +264,11 @@ def api_info(serialize: bool = True):
 
         @app.get("/config/", dependencies=[Depends(login_check)])
         @app.get("/config", dependencies=[Depends(login_check)])
-        def get_config():
-            return app.get_blocks().config
+        def get_config(request: fastapi.Request):
+            root_path = request.scope.get("root_path", "")
+            config = app.get_blocks().config
+            config["root"] = root_path
+            return config
 
         @app.get("/static/{path:path}")
         def static_resource(path: str):
@@ -778,7 +784,6 @@ def read_main():
         # Then run `uvicorn run:app` from the terminal and navigate to http://localhost:8000/gradio.
     """
     blocks.dev_mode = False
-    blocks.root = path[:-1] if path.endswith("/") else path
     blocks.config = blocks.get_config_file()
     blocks.validate_queue_settings()
     gradio_app = App.create_app(blocks)
diff --git a/gradio/test_data/blocks_configs.py b/gradio/test_data/blocks_configs.py
index c468d0dd7ac6c..cdcad71479b22 100644
--- a/gradio/test_data/blocks_configs.py
+++ b/gradio/test_data/blocks_configs.py
@@ -214,7 +214,6 @@
         "https://fonts.googleapis.com/css2?family=Source+Sans+Pro:wght@400;600&display=swap",
         "https://fonts.googleapis.com/css2?family=IBM+Plex+Mono:wght@400;600&display=swap",
     ],
-    "root": "",
     "theme": "default",
     "layout": {
         "id": 5,
@@ -524,7 +523,6 @@
         "https://fonts.googleapis.com/css2?family=Source+Sans+Pro:wght@400;600&display=swap",
         "https://fonts.googleapis.com/css2?family=IBM+Plex+Mono:wght@400;600&display=swap",
     ],
-    "root": "",
     "theme": "default",
     "layout": {
         "id": 5,