diff --git a/src/Grpc.Net.Client.Web/GrpcWebHandler.cs b/src/Grpc.Net.Client.Web/GrpcWebHandler.cs
index d56abda11..24f2e6a6d 100644
--- a/src/Grpc.Net.Client.Web/GrpcWebHandler.cs
+++ b/src/Grpc.Net.Client.Web/GrpcWebHandler.cs
@@ -20,6 +20,7 @@
 using System.Net;
 using System.Net.Http;
 using System.Net.Http.Headers;
+using System.Runtime.InteropServices;
 using System.Threading;
 using System.Threading.Tasks;
 using Grpc.Net.Client.Web.Internal;
@@ -39,6 +40,9 @@ public sealed class GrpcWebHandler : DelegatingHandler
     {
         internal const string WebAssemblyEnableStreamingResponseKey = "WebAssemblyEnableStreamingResponse";
 
+        // Internal and mutable for unit testing.
+        internal IOperatingSystem OperatingSystem { get; set; } = Internal.OperatingSystem.Instance;
+
         /// <summary>
         /// Gets or sets the HTTP version to use when making gRPC-Web calls.
         /// <para>
@@ -117,6 +121,11 @@ private async Task<HttpResponseMessage> SendAsyncCore(HttpRequestMessage request
         {
             request.Content = new GrpcWebRequestContent(request.Content!, GrpcWebMode);
 
+            if (OperatingSystem.IsBrowser)
+            {
+                FixBrowserUserAgent(request);
+            }
+
             // Set WebAssemblyEnableStreamingResponse to true on gRPC-Web request.
             // https://github.com/mono/mono/blob/a0d69a4e876834412ba676f544d447ec331e7c01/sdks/wasm/framework/src/System.Net.Http.WebAssemblyHttpHandler/WebAssemblyHttpHandler.cs#L149
             //
@@ -163,14 +172,29 @@ private async Task<HttpResponseMessage> SendAsyncCore(HttpRequestMessage request
             return response;
         }
 
+        private void FixBrowserUserAgent(HttpRequestMessage request)
+        {
+            const string userAgentHeader = "User-Agent";
+
+            // Remove the user-agent header and re-add it as x-user-agent.
+            // We don't want to override the browser's user-agent value.
+            // Consistent with grpc-web JS client which sends its header in x-user-agent.
+            // https://github.com/grpc/grpc-web/blob/2e3e8d2c501c4ddce5406ac24a637003eabae4cf/javascript/net/grpc/web/grpcwebclientbase.js#L323
+            if (request.Headers.TryGetValues(userAgentHeader, out var values))
+            {
+                request.Headers.Remove(userAgentHeader);
+                request.Headers.TryAddWithoutValidation("X-User-Agent", values);
+            }
+        }
+
         private static bool IsMatchingResponseContentType(GrpcWebMode mode, string? contentType)
         {
-            if (mode == Web.GrpcWebMode.GrpcWeb)
+            if (mode == GrpcWebMode.GrpcWeb)
             {
                 return CommonGrpcProtocolHelpers.IsContentType(GrpcWebProtocolConstants.GrpcWebContentType, contentType);
             }
 
-            if (mode == Web.GrpcWebMode.GrpcWebText)
+            if (mode == GrpcWebMode.GrpcWebText)
             {
                 return CommonGrpcProtocolHelpers.IsContentType(GrpcWebProtocolConstants.GrpcWebTextContentType, contentType);
             }
diff --git a/src/Grpc.Net.Client.Web/Internal/OperatingSystem.cs b/src/Grpc.Net.Client.Web/Internal/OperatingSystem.cs
new file mode 100644
index 000000000..bf44ffb0a
--- /dev/null
+++ b/src/Grpc.Net.Client.Web/Internal/OperatingSystem.cs
@@ -0,0 +1,39 @@
+#region Copyright notice and license
+
+// Copyright 2019 The gRPC Authors
+//
+// Licensed under the Apache License, Version 2.0 (the "License");
+// you may not use this file except in compliance with the License.
+// You may obtain a copy of the License at
+//
+//     http://www.apache.org/licenses/LICENSE-2.0
+//
+// Unless required by applicable law or agreed to in writing, software
+// distributed under the License is distributed on an "AS IS" BASIS,
+// WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+// See the License for the specific language governing permissions and
+// limitations under the License.
+
+#endregion
+
+using System.Runtime.InteropServices;
+
+namespace Grpc.Net.Client.Web.Internal
+{
+    internal interface IOperatingSystem
+    {
+        bool IsBrowser { get; }
+    }
+
+    internal class OperatingSystem : IOperatingSystem
+    {
+        public static readonly OperatingSystem Instance = new OperatingSystem();
+        
+        public bool IsBrowser { get; }
+                
+        private OperatingSystem()
+        {
+            IsBrowser = RuntimeInformation.IsOSPlatform(OSPlatform.Create("browser"));
+        }
+    }
+}
diff --git a/test/Grpc.Net.Client.Tests/Web/GrpcWebHandlerTests.cs b/test/Grpc.Net.Client.Tests/Web/GrpcWebHandlerTests.cs
index adc8e1eb2..4eace2677 100644
--- a/test/Grpc.Net.Client.Tests/Web/GrpcWebHandlerTests.cs
+++ b/test/Grpc.Net.Client.Tests/Web/GrpcWebHandlerTests.cs
@@ -18,6 +18,7 @@
 
 using System;
 using System.IO;
+using System.Linq;
 using System.Net;
 using System.Net.Http;
 using System.Net.Http.Headers;
@@ -110,6 +111,36 @@ public async Task SendAsync_GrpcCall_ResponseStreamingPropertySet()
             Assert.AreEqual(true, testHttpHandler.WebAssemblyEnableStreamingResponse);
         }
 
+        [Test]
+        public async Task SendAsync_GrpcCallInBrowser_UserAgentFixed()
+        {
+            // Arrange
+            var request = new HttpRequestMessage
+            {
+                Version = HttpVersion.Version20,
+                Content = new ByteArrayContent(Array.Empty<byte>())
+                {
+                    Headers = { ContentType = new MediaTypeHeaderValue("application/grpc") }
+                }
+            };
+            request.Headers.TryAddWithoutValidation("User-Agent", "TestUserAgent");
+            var testHttpHandler = new TestHttpHandler();
+            var grpcWebHandler = new GrpcWebHandler(GrpcWebMode.GrpcWeb, testHttpHandler);
+            grpcWebHandler.OperatingSystem = new TestOperatingSystem
+            {
+                IsBrowser = true
+            };
+            var messageInvoker = new HttpMessageInvoker(grpcWebHandler);
+
+            // Act
+            await messageInvoker.SendAsync(request, CancellationToken.None);
+
+            // Assert
+            Assert.AreEqual(false, testHttpHandler.RequestHeaders!.TryGetValues("user-agent", out _));
+            Assert.AreEqual(true, testHttpHandler.RequestHeaders!.TryGetValues("x-user-agent", out var values));
+            Assert.AreEqual("TestUserAgent", values!.Single());
+        }
+
         [Test]
         public async Task SendAsync_NonGrpcCall_ResponseStreamingPropertyNotSet()
         {
@@ -133,14 +164,21 @@ public async Task SendAsync_NonGrpcCall_ResponseStreamingPropertyNotSet()
             Assert.AreEqual(null, testHttpHandler.WebAssemblyEnableStreamingResponse);
         }
 
+        private class TestOperatingSystem : IOperatingSystem
+        {
+            public bool IsBrowser { get; set; }
+        }
+
         private class TestHttpHandler : HttpMessageHandler
         {
             public Version? RequestVersion { get; private set; }
             public bool? WebAssemblyEnableStreamingResponse { get; private set; }
+            public HttpRequestHeaders? RequestHeaders { get; private set; }
 
             protected override Task<HttpResponseMessage> SendAsync(HttpRequestMessage request, CancellationToken cancellationToken)
             {
                 RequestVersion = request.Version;
+                RequestHeaders = request.Headers;
 #pragma warning disable CS0618 // Type or member is obsolete
                 if (request.Properties.TryGetValue(GrpcWebHandler.WebAssemblyEnableStreamingResponseKey, out var enableStreaming))
 #pragma warning restore CS0618 // Type or member is obsolete