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the output of brie-count don't contain the .h5ad file #47

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liuyu988 opened this issue Jul 12, 2022 · 3 comments
Open

the output of brie-count don't contain the .h5ad file #47

liuyu988 opened this issue Jul 12, 2022 · 3 comments

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@liuyu988
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Hi Yuanhua,

my code is as follows:

brie-count -a genes.gff3 -S cell.bam -b barcodes.tsv.gz -o test2/ -p 15 -t any

this process ran for 15 hours, however, the result files don't contain the improtant .h5ad file.

Is anything i miss? thanks

@huangyh09
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How many genes and cells are you using? It's not fast but generally OK (~1h) for thousands of cells/genes.

Yuanhua

@huangyh09
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In v2.2.1, we now support output h5ad file if all genes only have two isoforms, no matter what splicing event types. you may consider re-run it with the new version.

Y

@liuyu988
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In v2.2.1, we now support output h5ad file if all genes only have two isoforms, no matter what splicing event types. you may consider re-run it with the new version.

Y

Thank you for your reply!
I have run this code for ~15 hours and the version i used is v2.2.0, I will try to use v2.2.1

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