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generate-settings
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generate-settings
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#!/usr/bin/env perl
use strict;
use warnings;
use File::Basename;
my $HELP = <<HELP;
Generate the initial settings.txt file for a specified genome.
usage: generate-settings genome
* genome = name (hg19, mm10, etc.) or exact dir (contains genome.fa and genes.gtf)
HELP
if (!$ARGV[0]) {
die $HELP;
}
main();
# main subroutine
sub main {
my $genome_arg = $ARGV[0];
# pipeline directory (the directory that this file is in)
my $pipeline_dir = dirname(__FILE__);
# settings file
my $settings_file = "settings.txt";
# check if the settings file already exists
if ( -e $settings_file ) {
die "\n\n ERROR: FILE $settings_file ALREADY EXISTS (DELETE IT TO GENERATE A NEW ONE) \n\n";
}
# set genome setting
my $genome_dir_setting;
if ($genome_arg =~ m/\//) {
# use directory if given
$genome_dir_setting = "GENOME-DIR|${genome_arg}\n";
}
else {
# use /gpfs/data/igorlab/ref if genome name is provided
$genome_dir_setting = "GENOME-DIR|/gpfs/data/igorlab/ref/${genome_arg}\n";
}
# save genome setting
open(my $fh, ">", $settings_file);
# print $fh $genome_setting;
print $fh $genome_dir_setting;
close $fh;
# get values to make sure they were set properly
my $settings_genome = `bash ${pipeline_dir}/scripts/get-set-setting.sh $settings_file GENOME-DIR`;
my $settings_fasta = `bash ${pipeline_dir}/scripts/get-set-setting.sh $settings_file REF-FASTA`;
# print values
print STDERR "\n";
print STDERR " REF DIR : $settings_genome \n";
print STDERR " REF FASTA : $settings_fasta \n";
}
# end