diff --git a/CHANGELOG.md b/CHANGELOG.md
index 68e11847ecfb..b2a023fa86dd 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -50,6 +50,7 @@
 - `[expect]` Avoid incorrect difference for subset when `toMatchObject` fails ([#9005](https://github.com/facebook/jest/pull/9005))
 - `[expect]` Consider all RegExp flags for equality ([#9167](https://github.com/facebook/jest/pull/9167))
 - `[expect]` [**BREAKING**] Consider primitives different from wrappers instantiated with `new` ([#9167](https://github.com/facebook/jest/pull/9167))
+- `[expect]` Prevent maintaining RegExp state between multiple tests ([#9289](https://github.com/facebook/jest/pull/9289))
 - `[expect]` Fix subsetEquality false circular reference detection ([#9322](https://github.com/facebook/jest/pull/9322))
 - `[jest-config]` Use half of the available cores when `watchAll` mode is enabled ([#9117](https://github.com/facebook/jest/pull/9117))
 - `[jest-config]` Fix Jest multi project runner still cannot handle exactly one project ([#8894](https://github.com/facebook/jest/pull/8894))
diff --git a/packages/expect/src/__tests__/matchers.test.js b/packages/expect/src/__tests__/matchers.test.js
index fb6223a9466f..f177a98ed932 100644
--- a/packages/expect/src/__tests__/matchers.test.js
+++ b/packages/expect/src/__tests__/matchers.test.js
@@ -1617,6 +1617,13 @@ describe('.toMatch()', () => {
   it('escapes strings properly', () => {
     jestExpect('this?: throws').toMatch('this?: throws');
   });
+
+  it('does not maintain RegExp state between calls', () => {
+    const regex = /[f]\d+/gi;
+    jestExpect('f123').toMatch(regex);
+    jestExpect('F456').toMatch(regex);
+    jestExpect(regex.lastIndex).toBe(0);
+  });
 });
 
 describe('.toHaveLength', () => {
diff --git a/packages/expect/src/matchers.ts b/packages/expect/src/matchers.ts
index ca51e52291ba..58c015992aeb 100644
--- a/packages/expect/src/matchers.ts
+++ b/packages/expect/src/matchers.ts
@@ -835,7 +835,7 @@ const matchers: MatchersObject = {
     const pass =
       typeof expected === 'string'
         ? received.includes(expected)
-        : expected.test(received);
+        : new RegExp(expected).test(received);
 
     const message = pass
       ? () =>