diff --git a/robottelo/host_helpers/satellite_mixins.py b/robottelo/host_helpers/satellite_mixins.py
index ef1edab74bf..7781bedf1d8 100644
--- a/robottelo/host_helpers/satellite_mixins.py
+++ b/robottelo/host_helpers/satellite_mixins.py
@@ -1,4 +1,5 @@
 import contextlib
+import io
 import random
 import re
 
@@ -125,7 +126,7 @@ def upload_manifest(self, org_id, manifest=None, interface='API', timeout=None):
             # Set the timeout to 1500 seconds to align with the API timeout.
             timeout = 1500000
         if interface == 'CLI':
-            if isinstance(manifest.content, bytes):
+            if isinstance(manifest.content, (bytes, io.BytesIO)):
                 self.put(f'{manifest.path}', f'{manifest.name}')
                 result = self.cli.Subscription.upload(
                     {'file': manifest.name, 'organization-id': org_id}, timeout=timeout
@@ -136,7 +137,7 @@ def upload_manifest(self, org_id, manifest=None, interface='API', timeout=None):
                     {'file': manifest.filename, 'organization-id': org_id}, timeout=timeout
                 )
         else:
-            if not isinstance(manifest, bytes):
+            if not isinstance(manifest, (bytes, io.BytesIO)):
                 manifest = manifest.content
             result = self.api.Subscription().upload(
                 data={'organization_id': org_id}, files={'content': manifest}