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No output from rnftools.mishmash.DwgSim #75

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zihhuafang opened this issue Apr 24, 2019 · 2 comments
Open

No output from rnftools.mishmash.DwgSim #75

zihhuafang opened this issue Apr 24, 2019 · 2 comments

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@zihhuafang
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Hi,
I would like to simulate illumina pair-end reads for samples with different coverage following the instructions here: https://bit.ly/2XKmXcV.

However, I encountered the following error.
RuleException:
KeyError in line 47 of /cluster/work/pausch/group_bin/anaconda3/envs/rnftools/lib/python3.6/site-packages/rnftools/mishmash/mishmash.snake:
's not in /cluster/work/pausch/fang/low_cov/sim/ARS-UCD1.2_Btau5.0.1Y.fa.'
File "/cluster/work/pausch/group_bin/anaconda3/envs/rnftools/lib/python3.6/site-packages/rnftools/mishmash/mishmash.snake", line 47, in __rule_5
File "/cluster/work/pausch/group_bin/anaconda3/envs/rnftools/lib/python3.6/site-packages/rnftools/mishmash/Source.py", line 153, in create_fa
File "/cluster/work/pausch/group_bin/anaconda3/envs/rnftools/lib/python3.6/site-packages/pyfaidx/init.py", line 1016, in getitem
File "/cluster/work/pausch/group_bin/anaconda3/envs/rnftools/lib/python3.6/concurrent/futures/thread.py", line 56, in run
Removing output files of failed job 6 since they might be corrupted:
/cluster/work/pausch/fang/low_cov/sim/simulation_with_coverage_control/004/reference.fa
Shutting down, this might take some time.

I am not sure how to fix this error. I'd appreciate any advice. Thanks!

@karel-brinda
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karel-brinda commented Apr 24, 2019

Could you please upload your Snakefile and coverage.txt? It would also be useful to see your ref.fa.fai (can be obtained by samtools faidx ref.fa).

@zihhuafang
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I put all the files here: https://www.dropbox.com/sh/455d8m45vqderrn/AACKm5QXBm2mh-2yBZDsNSnVa?dl=0

I had issue with csv, so I installed pandas and replace commends related to csv. Will this cause the issue?

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