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add notes for all EAGER run
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ktmeaton committed Jun 17, 2020
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Expand Up @@ -240,6 +240,8 @@ Run EAGER pipeline

mkdir test/eager;
cp ~/.nextflow/assets/nf-core/eager/assets/multiqc_config.yaml ./multiqc_config_custom.yaml
conda activate eager-env;

nextflow run nf-core/eager -r dev \
--input test/sqlite_import/metadata_sra_eager.tsv \
--outdir test/eager \
Expand All @@ -249,4 +251,42 @@ Run EAGER pipeline
--preserve5p \
--mergedonly \
--mapper bwaaln \
--bwaalnn 0.01 \
--bwaalnl 16 \
--run_bam_filtering \
--bam_mapping_quality_threshold 30 \
--bam_discard_unmapped \
--bam_unmapped_type discard \
-resume 35a03fea-8f18-4174-b273-05ee7cbfaaa0

Repeat but include Barcelona3031 (SRARunAccession = 'ERR1368878')

::

nextflow run pipeline.nf \
--sqlite results/ncbimeta_db/update/latest/output/database/yersinia_pestis_db.sqlite \
--outdir test \
--sqlite_select_command_sra "\"SELECT BioSampleAccession,SRARunAccession,SRALibraryLayout,SRAFileURL FROM Master WHERE (SRARunAccession = 'SRR341961' OR SRARunAccession = 'ERR1368878')\"" \
--max_datasets_assembly 2000 \
--max_datasets_sra 2000 \
--skip_assembly_download \
--skip_sra_download \
--skip_reference_detect_repeats \
--skip_reference_detect_low_complexity \
-resume ab34c580-164d-4420-9e6f-a5aa7aa1dd05

Repeat but include everything marked for EAGER

::

nextflow run pipeline.nf \
--sqlite results/ncbimeta_db/update/latest/output/database/yersinia_pestis_db.sqlite \
--outdir test \
--sqlite_select_command_sra "\"SELECT BioSampleAccession,SRARunAccession,SRALibraryLayout,SRAFileURL FROM Master WHERE (BioSampleComment LIKE '%EAGER%')\"" \
--max_datasets_assembly 2000 \
--max_datasets_sra 2000 \
--skip_assembly_download \
--skip_sra_download \
--skip_reference_detect_repeats \
--skip_reference_detect_low_complexity \
-resume ab34c580-164d-4420-9e6f-a5aa7aa1dd05

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