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I was wondering whether you feel the map-ont preset is still the best option for the latest (R10.4.1) nanopore data basecalled with super high accuracy (sup) models? For example, the manpage says this preset of for noisy long reads of ~10% error rate. Some recent nanopore samples I am working with are showing a median (gap-compressed) identity of 99.3%.
I noticed there is the PacBio presets (specifically hifi), but it doesn't mention what error rate those are targeted at and I am not sure if they would be appropriate?
The text was updated successfully, but these errors were encountered:
Hello, did you figure this out? I am wondering the same. I have noticed that the assembler Flye uses an adjusted -k to -k17 instead of the default value 15 that comes with the ont preset.
You can see Heng Li answers here: #1127 (comment)
In short, map-ont should work but he wants to improve minimap2
I was wondering whether you feel the
map-ont
preset is still the best option for the latest (R10.4.1) nanopore data basecalled with super high accuracy (sup) models? For example, the manpage says this preset of for noisy long reads of ~10% error rate. Some recent nanopore samples I am working with are showing a median (gap-compressed) identity of 99.3%.I noticed there is the PacBio presets (specifically hifi), but it doesn't mention what error rate those are targeted at and I am not sure if they would be appropriate?
The text was updated successfully, but these errors were encountered: