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MD string for second reads #226

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hirokiuedaRcast opened this issue Sep 5, 2018 · 1 comment · May be fixed by #1223
Open

MD string for second reads #226

hirokiuedaRcast opened this issue Sep 5, 2018 · 1 comment · May be fixed by #1223
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@hirokiuedaRcast
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Hi

When I excute mappy with paired ends like

aligner.map(seq1,seq2, MD=True):

MD string for second reads is wrong.
for example for 100nt length perfect match, it reports.
0T2A0G1C0T0C0A0T0T1T1T0T0G0C0C1A1G0C0T0G0G0A0G0T0G0C1G0T0G0G0T0G0T0G0A1C0T0C0G0G0C0T0C0A0C0T0G0C0A1C0C0C0C0C0A0A0C0C0C1C0A0C0T1C0C0C0A0G2T1A0A1C0G0A1T1T0C0C0T0G0C0C

I think, it maybe that second string is not reverse complemented when generating MD string.
(I put read FR oriented as instructed and mapping just works fine.)

Thanks in Advance.

@lh3 lh3 added the bug label Sep 7, 2018
@y9c
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y9c commented Jun 18, 2024

This bug still exists in the latest version of the mappy package. I believe it is a crucial issue, and users should be warned about it if it is difficult to fix.

@y9c y9c linked a pull request Jun 18, 2024 that will close this issue
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3 participants