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If an invalid RG line is passed to option -R, minimap detects the error but keeps going without adding RG tags. Maybe better to make it quit? E.g:
-R
minimap2 -V 2.17-r941 minimap2 -R 'foobar' -a some.fasta some.fastq > /dev/null [M::mm_idx_gen::2.132*1.00] collected minimizers [M::mm_idx_gen::2.469*1.25] sorted minimizers [ERROR] the read group line is not started with @RG <============ [M::main::2.469*1.25] loaded/built the index for 36 target sequence(s) [M::mm_mapopt_update::2.633*1.24] mid_occ = 32 [M::mm_idx_stat] kmer size: 15; skip: 10; is_hpc: 0; #seq: 36 [M::mm_idx_stat::2.762*1.23] distinct minimizers: 5072250 (89.73% are singletons); average occurrences: 1.216; average spacing: 5.327 [M::worker_pipeline::8.651*2.27] mapped 4000 sequences [M::main] Version: 2.17-r941 [M::main] CMD: minimap2 -R foobar -a ref/TriTrypDB-45_LmajorFriedlin_Genome.fasta /export/projects/III-data/wcmp_bioinformatics/db291g/data/20190906_catarina_nanopore/20190902_1335_MN23371_FAK87904_27b810d7/fastq_pass/FAK87904_a513394c0ef96eff721553a5edf39959f27553c9_44.fastq.gz [M::main] Real time: 8.718 sec; CPU: 19.723 sec; Peak RSS: 0.641 GB
The text was updated successfully, but these errors were encountered:
Maybe better to make it quit?
Fair enough. I will consider this in the next release.
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If an invalid RG line is passed to option
-R
, minimap detects the error but keeps going without adding RG tags. Maybe better to make it quit? E.g:The text was updated successfully, but these errors were encountered: