diff --git a/changelog.d/12141.bugfix b/changelog.d/12141.bugfix
new file mode 100644
index 000000000000..03a2507e2ce6
--- /dev/null
+++ b/changelog.d/12141.bugfix
@@ -0,0 +1 @@
+Fix a bug introduced in Synapse 1.54.0rc1 preventing the new module callbacks introduced in this release from being registered by modules.
diff --git a/synapse/events/third_party_rules.py b/synapse/events/third_party_rules.py
index dd3104faf3ac..ede72ee87631 100644
--- a/synapse/events/third_party_rules.py
+++ b/synapse/events/third_party_rules.py
@@ -174,7 +174,9 @@ def register_third_party_rules_callbacks(
         ] = None,
         on_new_event: Optional[ON_NEW_EVENT_CALLBACK] = None,
         on_profile_update: Optional[ON_PROFILE_UPDATE_CALLBACK] = None,
-        on_deactivation: Optional[ON_USER_DEACTIVATION_STATUS_CHANGED_CALLBACK] = None,
+        on_user_deactivation_status_changed: Optional[
+            ON_USER_DEACTIVATION_STATUS_CHANGED_CALLBACK
+        ] = None,
     ) -> None:
         """Register callbacks from modules for each hook."""
         if check_event_allowed is not None:
@@ -199,8 +201,10 @@ def register_third_party_rules_callbacks(
         if on_profile_update is not None:
             self._on_profile_update_callbacks.append(on_profile_update)
 
-        if on_deactivation is not None:
-            self._on_user_deactivation_status_changed_callbacks.append(on_deactivation)
+        if on_user_deactivation_status_changed is not None:
+            self._on_user_deactivation_status_changed_callbacks.append(
+                on_user_deactivation_status_changed,
+            )
 
     async def check_event_allowed(
         self, event: EventBase, context: EventContext
diff --git a/synapse/module_api/__init__.py b/synapse/module_api/__init__.py
index 7e469318695e..c42eeedd87ae 100644
--- a/synapse/module_api/__init__.py
+++ b/synapse/module_api/__init__.py
@@ -59,6 +59,8 @@
     CHECK_VISIBILITY_CAN_BE_MODIFIED_CALLBACK,
     ON_CREATE_ROOM_CALLBACK,
     ON_NEW_EVENT_CALLBACK,
+    ON_PROFILE_UPDATE_CALLBACK,
+    ON_USER_DEACTIVATION_STATUS_CHANGED_CALLBACK,
 )
 from synapse.handlers.account_validity import (
     IS_USER_EXPIRED_CALLBACK,
@@ -281,6 +283,10 @@ def register_third_party_rules_callbacks(
             CHECK_VISIBILITY_CAN_BE_MODIFIED_CALLBACK
         ] = None,
         on_new_event: Optional[ON_NEW_EVENT_CALLBACK] = None,
+        on_profile_update: Optional[ON_PROFILE_UPDATE_CALLBACK] = None,
+        on_user_deactivation_status_changed: Optional[
+            ON_USER_DEACTIVATION_STATUS_CHANGED_CALLBACK
+        ] = None,
     ) -> None:
         """Registers callbacks for third party event rules capabilities.
 
@@ -292,6 +298,8 @@ def register_third_party_rules_callbacks(
             check_threepid_can_be_invited=check_threepid_can_be_invited,
             check_visibility_can_be_modified=check_visibility_can_be_modified,
             on_new_event=on_new_event,
+            on_profile_update=on_profile_update,
+            on_user_deactivation_status_changed=on_user_deactivation_status_changed,
         )
 
     def register_presence_router_callbacks(