From 9be3530d2ce84e29c68d4f30eb8107df46127b21 Mon Sep 17 00:00:00 2001 From: maxibor Date: Wed, 7 Mar 2018 13:55:40 +0100 Subject: [PATCH] adding documentation and renaming --- README.md | 62 ++++++++++++++++++++++++++++++-- adrsm_multi.py => adrsm | 4 +-- adrsm_single.py => adrsm_single | 4 +-- img/logo_adrsm.png | Bin 0 -> 72457 bytes 4 files changed, 64 insertions(+), 6 deletions(-) rename adrsm_multi.py => adrsm (97%) mode change 100644 => 100755 rename adrsm_single.py => adrsm_single (95%) mode change 100644 => 100755 create mode 100644 img/logo_adrsm.png diff --git a/README.md b/README.md index 947a53a..6e26127 100644 --- a/README.md +++ b/README.md @@ -1,2 +1,60 @@ -# adrsm -Ancient DNA Read Simulator for Metagenomic + + +# Introduction +ADRSM (Ancient DNA Read Simulator for Metagenomics) is a tool designed to simulate the paired-end sequencing of a metagenomic community. ADRSM allows you to control precisely the amount of DNA from each organism in the community, which can be used to benchmark different metagenomics methods. + +# Dependencies +- [Conda](https://conda.io/miniconda.html) + +# Installation + +``` +conda install -c maxibor adrsm +``` + +# Usage + +``` +adrsm -d path/to/genome/directory configFile.txt +``` + +# Help + +``` +maxime@gph:~$ adrsm --help +usage: ADRSM [-h] [-d DIRECTORY] [-r READLENGTH] [-l LENSTDEV] [-fwd FWDADAPT] + [-rev REVADAPT] [-e ERROR] [-o OUTPUT] [-s STATS] + confFile + +Ancient DNA Read Simulator for Metagenomics + +positional arguments: + confFile path to configuration file + +optional arguments: + -h, --help show this help message and exit + -d DIRECTORY path to genome directory. Default = . + -r READLENGTH Average read length. Default = 76 + -l LENSTDEV Insert length standard deviation. Default = 10 + -fwd FWDADAPT Forward adaptor. Default = AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC + NNNNNNATCTCGTATGCCGTCTTCTGCTTG + -rev REVADAPT Reverse adaptor. Default = + AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT + -e ERROR Illumina sequecing error. Default = 0.01 + -o OUTPUT Output file basename. Default = ./metagenome.* + -s STATS Statistic file. Default = stats.csv + +``` + +## Genome directory +Each genome `fasta` file must be names after the name of the organism. (ex: `Bacillus_anthracis_genome.fa`) + +## Configuration file (`confFile`) +The configuration file is a `.csv` file describing, one line per genome, the mean insert size, and the expected genome coverage. +Example [short_genome_list.csv](./short_genome_list.csv): + +``` +genome, insert_size, coverage +Agrobacterium_tumefaciens_genome.fa, 47 , 0.1 +Bacillus_anthracis_genome.fa, 48, 0.2 +``` diff --git a/adrsm_multi.py b/adrsm old mode 100644 new mode 100755 similarity index 97% rename from adrsm_multi.py rename to adrsm index 2eadb51..0ccb118 --- a/adrsm_multi.py +++ b/adrsm @@ -8,8 +8,8 @@ def _get_args(): '''This function parses and return arguments passed in''' parser = argparse.ArgumentParser( - prog='MetaBenReadSim', - description='Metagenomic Benchmarking Read Simulator for ancient DNA') + prog='ADRSM', + description='Ancient DNA Read Simulator for Metagenomics') parser.add_argument('confFile', help="path to configuration file") parser.add_argument( '-d', diff --git a/adrsm_single.py b/adrsm_single old mode 100644 new mode 100755 similarity index 95% rename from adrsm_single.py rename to adrsm_single index 38c2771..70e3b80 --- a/adrsm_single.py +++ b/adrsm_single @@ -9,8 +9,8 @@ def _get_args(): '''This function parses and return arguments passed in''' parser = argparse.ArgumentParser( - prog='MetaBenReadSim', - description='Metagenomic Benchmarking Read Simulator for ancient DNA') + prog='ADRSM', + description='Ancient DNA Read Simulator for Metagenomics') parser.add_argument('infile', help="path to reference fasta file") parser.add_argument( '-n', diff --git a/img/logo_adrsm.png b/img/logo_adrsm.png new file mode 100644 index 0000000000000000000000000000000000000000..33dc34a722598ef336fa7fb39f92b94f95e710df GIT binary patch literal 72457 zcmcG#Wo(>H(=Iq>W@e_CV`hvUGdpHxW@cuNnJIS6%rP^JnVB6kv%T}2_kDC$+D|&t zu6F*++Qc+$K2_7FF001CKeHT{-03fgd0B|W-Xiy6mS^+EQ3({Fs zN);CL@qsmo06pWmeA94Iu{U#ZH*_)unA_Rgnld^YJDHl=Ia}DfTtajU0sy1{DRB{1 zkL+U5zzrRZro4X4&L^c|A 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