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mRNA-seq DESeq2 - sample names with dot are truncated #789

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mirax87 opened this issue Sep 16, 2021 · 1 comment
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mRNA-seq DESeq2 - sample names with dot are truncated #789

mirax87 opened this issue Sep 16, 2021 · 1 comment

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@mirax87
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mirax87 commented Sep 16, 2021

Bug report:

Sample names in featureCounts/counts.tsv contain the prefix up to the first dot in the file name. That means, if your sample names natively contains a dot, it may become truncated and the sample sheets for DESeq2 will fail.

Would it be possible to allow for sample names with dots? I guess, this will be found somewhere in the script that collects the featureCounts/*.txt

My guess is the following line requires and update:

https://github.com/maxplanck-ie/snakepipes/blob/master/snakePipes/shared/rscripts/merge_featureCounts.R#L18

@katsikora
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Hi Michael,

I'm not sure we support dots in sample names. These might cause issues also in R or be transformed to something that will indeed fail.
Would you have an example to test on?

Best,
Katarzyna

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