BD Rhapsody + Nanopore Sequencing

[skyng22003]

Would it be possible to modify the "generic-ont-with-umi" protocol to include the BD Rhapsody Cell barcodes for TCR / mRNA analysis?

Thanks,
Sky

[mizraelson]

Hi, which BD protocol did you use to generate the data? Also do you have ONT adapters trimmed from the FASTQ reads? I can create a custom preset for your dataset but it would help if you can share a subsample of reads from the original fastq file for me to test. You can write us at support@milaboratories.com.

[skyng22003]

Thank you for replying, I have written to support@milaboratories.com with the details.