diff --git a/packages/nodes-base/nodes/Aws/S3/V2/AwsS3V2.node.ts b/packages/nodes-base/nodes/Aws/S3/V2/AwsS3V2.node.ts
index 74f654f7d1f8e..7f198797de4fb 100644
--- a/packages/nodes-base/nodes/Aws/S3/V2/AwsS3V2.node.ts
+++ b/packages/nodes-base/nodes/Aws/S3/V2/AwsS3V2.node.ts
@@ -300,7 +300,7 @@ export class AwsS3V2 implements INodeType {
 							headers['x-amz-request-payer'] = 'requester';
 						}
 						if (additionalFields.parentFolderKey) {
-							path = `/${additionalFields.parentFolderKey}${folderName}/`;
+							path = `${basePath}/${additionalFields.parentFolderKey}/${folderName}/`;
 						}
 						if (additionalFields.storageClass) {
 							headers['x-amz-storage-class'] = snakeCase(
@@ -778,7 +778,7 @@ export class AwsS3V2 implements INodeType {
 						const additionalFields = this.getNodeParameter('additionalFields', i);
 						const tagsValues = (this.getNodeParameter('tagsUi', i) as IDataObject)
 							.tagsValues as IDataObject[];
-						let path = `${basePath}/`;
+						let path = `${basePath}/${fileName}`;
 						let body;
 
 						const multipartHeaders: IDataObject = {};
@@ -788,9 +788,7 @@ export class AwsS3V2 implements INodeType {
 							neededHeaders['x-amz-request-payer'] = 'requester';
 						}
 						if (additionalFields.parentFolderKey) {
-							path = `${additionalFields.parentFolderKey}/${fileName}`;
-						} else {
-							path = `${fileName}`;
+							path = `${basePath}/${additionalFields.parentFolderKey}/${fileName}`;
 						}
 						if (additionalFields.storageClass) {
 							multipartHeaders['x-amz-storage-class'] = snakeCase(
@@ -877,7 +875,7 @@ export class AwsS3V2 implements INodeType {
 									this,
 									servicePath,
 									'POST',
-									`/${path}?uploads`,
+									`${path}?uploads`,
 									body,
 									qs,
 									{ ...neededHeaders, ...multipartHeaders },
@@ -899,7 +897,7 @@ export class AwsS3V2 implements INodeType {
 											this,
 											servicePath,
 											'PUT',
-											`/${path}?partNumber=${part}&uploadId=${uploadId}`,
+											`${path}?partNumber=${part}&uploadId=${uploadId}`,
 											chunk,
 											qs,
 											listHeaders,
@@ -913,7 +911,7 @@ export class AwsS3V2 implements INodeType {
 												this,
 												servicePath,
 												'DELETE',
-												`/${path}?uploadId=${uploadId}`,
+												`${path}?uploadId=${uploadId}`,
 											);
 										} catch (err) {
 											throw new NodeOperationError(this.getNode(), err as Error);
@@ -926,7 +924,7 @@ export class AwsS3V2 implements INodeType {
 									this,
 									servicePath,
 									'GET',
-									`/${path}?max-parts=${900}&part-number-marker=0&uploadId=${uploadId}`,
+									`${path}?max-parts=${900}&part-number-marker=0&uploadId=${uploadId}`,
 									'',
 									qs,
 									{ ...neededHeaders },
@@ -978,7 +976,7 @@ export class AwsS3V2 implements INodeType {
 									this,
 									servicePath,
 									'POST',
-									`/${path}?uploadId=${uploadId}`,
+									`${path}?uploadId=${uploadId}`,
 									data,
 									qs,
 									{
@@ -1015,7 +1013,7 @@ export class AwsS3V2 implements INodeType {
 									this,
 									servicePath,
 									'PUT',
-									`/${path || binaryPropertyData.fileName}`,
+									path,
 									body,
 									qs,
 									headers,
@@ -1043,7 +1041,7 @@ export class AwsS3V2 implements INodeType {
 								this,
 								servicePath,
 								'PUT',
-								`/${path}`,
+								path,
 								body,
 								qs,
 								{ ...headers },
diff --git a/packages/nodes-base/nodes/Aws/S3/test/V2/AwsS3.file.upload.V2.workflow.json b/packages/nodes-base/nodes/Aws/S3/test/V2/AwsS3.file.upload.V2.workflow.json
index a54d819ef6584..5d88217684631 100644
--- a/packages/nodes-base/nodes/Aws/S3/test/V2/AwsS3.file.upload.V2.workflow.json
+++ b/packages/nodes-base/nodes/Aws/S3/test/V2/AwsS3.file.upload.V2.workflow.json
@@ -23,7 +23,7 @@
 		{
 			"parameters": {
 				"operation": "upload",
-				"bucketName": "bucket",
+				"bucketName": "buc.ket",
 				"fileName": "binary.json",
 				"additionalFields": {}
 			},
diff --git a/packages/nodes-base/nodes/Aws/S3/test/V2/AwsS3.node.test.ts b/packages/nodes-base/nodes/Aws/S3/test/V2/AwsS3.node.test.ts
index 2f3b0516c8cbc..7d4da16178d9d 100644
--- a/packages/nodes-base/nodes/Aws/S3/test/V2/AwsS3.node.test.ts
+++ b/packages/nodes-base/nodes/Aws/S3/test/V2/AwsS3.node.test.ts
@@ -14,11 +14,11 @@ describe('Test S3 V2 Node', () => {
 			await initBinaryDataService();
 
 			nock.disableNetConnect();
-			mock = nock('https://bucket.s3.eu-central-1.amazonaws.com');
+			mock = nock('https://s3.eu-central-1.amazonaws.com/buc.ket');
 		});
 
 		beforeEach(async () => {
-			mock.get('/?location').reply(
+			mock.get('?location').reply(
 				200,
 				`<?xml version="1.0" encoding="UTF-8"?>
 				<LocationConstraint>