diff --git a/subworkflows/local/germline_variant_calling.nf b/subworkflows/local/germline_variant_calling.nf index ee0d45c72..9096f68b8 100644 --- a/subworkflows/local/germline_variant_calling.nf +++ b/subworkflows/local/germline_variant_calling.nf @@ -18,14 +18,14 @@ workflow GERMLINE_VARIANT_CALLING { bwa // channel: [mandatory] bwa dbsnp // channel: [mandatory] dbsnp dbsnp_tbi // channel: [mandatory] dbsnp_tbi - known_sites - known_sites_tbi dict // channel: [mandatory] dict fasta // channel: [mandatory] fasta fasta_fai // channel: [mandatory] fasta_fai intervals // channel: [mandatory] intervals/target regions intervals_bed_gz_tbi // channel: [mandatory] intervals/target regions index zipped and indexed intervals_bed_combined // channel: [mandatory] intervals/target regions in one file unzipped + known_sites + known_sites_tbi // joint_germline // val: true/false on whether to run joint_germline calling, only works in combination with haplotypecaller at the moment main: diff --git a/subworkflows/local/tumor_variant_calling.nf b/subworkflows/local/tumor_variant_calling.nf index a2dd68cd2..f9c8b44f0 100644 --- a/subworkflows/local/tumor_variant_calling.nf +++ b/subworkflows/local/tumor_variant_calling.nf @@ -16,21 +16,21 @@ workflow TUMOR_ONLY_VARIANT_CALLING { take: tools // Mandatory, list of tools to apply cram_recalibrated // channel: [mandatory] cram + bwa // channel: [optional] bwa + chr_files dbsnp // channel: [mandatory] dbsnp dbsnp_tbi // channel: [mandatory] dbsnp_tbi dict // channel: [mandatory] dict fasta // channel: [mandatory] fasta fasta_fai // channel: [mandatory] fasta_fai + germline_resource // channel: [optional] germline_resource + germline_resource_tbi // channel: [optional] germline_resource_tbi intervals // channel: [mandatory] intervals/target regions intervals_bed_gz_tbi // channel: [mandatory] intervals/target regions index zipped and indexed intervals_bed_combined // channel: [mandatory] intervals/target regions in one file unzipped - germline_resource // channel: [optional] germline_resource - germline_resource_tbi // channel: [optional] germline_resource_tbi + mappability panel_of_normals // channel: [optional] panel_of_normals panel_of_normals_tbi // channel: [optional] panel_of_normals_tbi - chr_files - mappability - bwa // channel: [optional] bwa main: diff --git a/workflows/sarek.nf b/workflows/sarek.nf index e371c82f2..28750c994 100644 --- a/workflows/sarek.nf +++ b/workflows/sarek.nf @@ -792,35 +792,35 @@ workflow SAREK { [], dbsnp, dbsnp_tbi, - known_sites, - known_sites_tbi, dict, fasta, fasta_fai, intervals, intervals_bed_gz_tbi, - intervals_bed_combined) + intervals_bed_combined, + known_sites, + known_sites_tbi) // params.joint_germline) // TUMOR ONLY VARIANT CALLING TUMOR_ONLY_VARIANT_CALLING( params.tools, cram_variant_calling_tumor_only, + [], + chr_files, dbsnp, dbsnp_tbi, dict, fasta, fasta_fai, + germline_resource, + germline_resource_tbi, intervals, intervals_bed_gz_tbi, intervals_bed_combined, - germline_resource, - germline_resource_tbi, - pon, - pon_tbi, - chr_files, mappability, - [] + pon, + pon_tbi ) // PAIR VARIANT CALLING