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Display sentieon dedup metrics in multiqc #1123

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Jun 21, 2023
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31 changes: 31 additions & 0 deletions assets/multiqc_config.yml
Original file line number Diff line number Diff line change
Expand Up @@ -66,7 +66,38 @@ sample_names_replace:
"\\.[0-9]{4}$": ".md" # should match ".0001" but only at the end of strings for module Markduplicates/EstimateLibraryComplexity
module: picard

custom_data:
dedup_metrics:
id: 'dedup_metrics'
section_name: 'Sentieon Dedup Metrics'
plot_type: 'table'
pconfig:
id: 'dedup_metrics'
namespace: 'Sentieon Dedup Metrics'
headers:
LIBRARY:
description: 'LIBRARY'
UNPAIRED_READS_EXAMINED:
description: 'UNPAIRED_READS_EXAMINE'
READ_PAIRS_EXAMINED:
description: 'READ_PAIRS_EXAMINED'
SECONDARY_OR_SUPPLEMENTARY_RDS:
description: 'SECONDARY_OR_SUPPLEMENTARY_RDS'
UNMAPPED_READS:
description: 'UNMAPPED_READS'
UNPAIRED_READ_DUPLICATES:
description: 'UNPAIRED_READ_DUPLICATES'
READ_PAIR_DUPLICATES:
description: 'READ_PAIR_DUPLICATES'
READ_PAIR_OPTICAL_DUPLICATES:
description: 'READ_PAIR_OPTICAL_DUPLICATES'
PERCENT_DUPLICATION:
description: 'PERCENT_DUPLICATION'
ESTIMATED_LIBRARY_SIZE:
description: 'ESTIMATED_LIBRARY_SIZE'
sp:
snpeff:
contents: "SnpEff_version"
max_filesize: 5000000
dedup_metrics:
fn: "*.metrics.multiqc.tsv"
2 changes: 1 addition & 1 deletion conf/modules/sentieon_dedup.config
Original file line number Diff line number Diff line change
Expand Up @@ -28,7 +28,7 @@ process {
[
mode: params.publish_dir_mode,
path: { "${params.outdir}/reports/" },
pattern: "*metrics",
pattern: "*{metrics,metrics.multiqc.tsv}",
saveAs: { !(params.skip_tools && params.skip_tools.split(',').contains('sentieon_dedup_report')) ? "sentieon_dedup/${meta.id}/${it}" : null}
]
]
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3 changes: 2 additions & 1 deletion modules.json
Original file line number Diff line number Diff line change
Expand Up @@ -384,7 +384,8 @@
"sentieon/dedup": {
"branch": "master",
"git_sha": "2cb2012b268b3502ac74463e510e76be99e2cdbf",
"installed_by": ["modules"]
"installed_by": ["modules"],
"patch": "modules/nf-core/sentieon/dedup/sentieon-dedup.diff"
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},
"sentieon/gvcftyper": {
"branch": "master",
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4 changes: 4 additions & 0 deletions modules/nf-core/sentieon/dedup/main.nf

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23 changes: 23 additions & 0 deletions modules/nf-core/sentieon/dedup/sentieon-dedup.diff

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1 change: 1 addition & 0 deletions subworkflows/local/bam_sentieon_dedup/main.nf
Original file line number Diff line number Diff line change
Expand Up @@ -34,6 +34,7 @@ workflow BAM_SENTIEON_DEDUP {

// Gather all reports generated
reports = reports.mix(SENTIEON_DEDUP.out.metrics)
reports = reports.mix(SENTIEON_DEDUP.out.metrics_multiqc_tsv)
reports = reports.mix(SENTIEON_DEDUP.out.score)
reports = reports.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.reports)

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2 changes: 2 additions & 0 deletions tests/test_sentieon_dedup_from_bam.yml
Original file line number Diff line number Diff line change
Expand Up @@ -13,6 +13,8 @@
- path: results/csv/recalibrated.csv
md5sum: 1888a924bc70bd80165a96ad641e22d6
- path: results/multiqc
- path: results/multiqc/multiqc_report.html
contains: ["Sentieon Dedup Metrics", "PERCENT_DUPLICATION", "ESTIMATED_LIBRARY_SIZE"]
- path: results/preprocessing/sentieon_dedup/test/test.md.cram
# binary changes md5sums on reruns
- path: results/preprocessing/sentieon_dedup/test/test.md.cram.crai
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2 changes: 2 additions & 0 deletions tests/test_sentieon_dedup_from_cram.yml
Original file line number Diff line number Diff line change
Expand Up @@ -13,6 +13,8 @@
- path: results/csv/recalibrated.csv
md5sum: 1888a924bc70bd80165a96ad641e22d6
- path: results/multiqc
- path: results/multiqc/multiqc_report.html
contains: ["Sentieon Dedup Metrics", "PERCENT_DUPLICATION", "ESTIMATED_LIBRARY_SIZE"]
- path: results/preprocessing/sentieon_dedup/test/test.md.cram
# binary changes md5sums on reruns
- path: results/preprocessing/sentieon_dedup/test/test.md.cram.crai
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