diff --git a/CHANGELOG.md b/CHANGELOG.md
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--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -32,6 +32,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#582](https://github.com/nf-core/sarek/pull/582) - Added option `--vep_out_format` for setting the format of the output-file from VEP to `json`, `tab` or `vcf` (default)
 - [#594](https://github.com/nf-core/sarek/pull/594) - Add parameter `--save_output_as_bam` to allow output of result files in BAM format
 - [#600](https://github.com/nf-core/sarek/pull/600) - Added description for UMI related params in schema
+- [#597](https://github.com/nf-core/sarek/pull/597) - Added tiddit for tumor variant calling
 
 ### Changed
 
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+           style="color:#000000;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:medium;line-height:normal;font-family:sans-serif;font-variant-ligatures:normal;font-variant-position:normal;font-variant-caps:normal;font-variant-numeric:normal;font-variant-alternates:normal;font-feature-settings:normal;text-indent:0;text-align:start;text-decoration:none;text-decoration-line:none;text-decoration-style:solid;text-decoration-color:#000000;letter-spacing:normal;word-spacing:normal;text-transform:none;writing-mode:lr-tb;direction:ltr;text-orientation:mixed;dominant-baseline:auto;baseline-shift:baseline;text-anchor:start;white-space:normal;shape-padding:0;clip-rule:nonzero;display:inline;overflow:visible;visibility:visible;opacity:1;isolation:auto;mix-blend-mode:normal;color-interpolation:sRGB;color-interpolation-filters:linearRGB;solid-color:#000000;solid-opacity:1;vector-effect:none;fill:#ffffff;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:7.8741;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-dashoffset:0;stroke-opacity:1;color-rendering:auto;image-rendering:auto;shape-rendering:auto;text-rendering:auto;enable-background:accumulate"
+           d="m 575.52539,395.2832 v 22.44922 h 7.5 V 395.2832 Z"
+           transform="matrix(0.9375,0,0,0.9375,15.436598,-660.07976)"
+           id="path5682-8-3"
+           inkscape:connector-curvature="0"
+           sodipodi:nodetypes="ccccc" />
+        <path
+           style="color:#000000;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:medium;line-height:normal;font-family:sans-serif;font-variant-ligatures:normal;font-variant-position:normal;font-variant-caps:normal;font-variant-numeric:normal;font-variant-alternates:normal;font-feature-settings:normal;text-indent:0;text-align:start;text-decoration:none;text-decoration-line:none;text-decoration-style:solid;text-decoration-color:#000000;letter-spacing:normal;word-spacing:normal;text-transform:none;writing-mode:lr-tb;direction:ltr;text-orientation:mixed;dominant-baseline:auto;baseline-shift:baseline;text-anchor:start;white-space:normal;shape-padding:0;clip-rule:nonzero;display:inline;overflow:visible;visibility:visible;opacity:1;isolation:auto;mix-blend-mode:normal;color-interpolation:sRGB;color-interpolation-filters:linearRGB;solid-color:#000000;solid-opacity:1;vector-effect:none;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:4.00004;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-dashoffset:0;stroke-opacity:1;color-rendering:auto;image-rendering:auto;shape-rendering:auto;text-rendering:auto;enable-background:accumulate"
+           d="m 571.52539,397.2832 v 18.44922 h 4 V 397.2832 Z m 11.5,0 v 18.44922 h 4 V 397.2832 Z"
+           transform="matrix(0.9375,0,0,0.9375,15.436598,-660.07976)"
+           id="path5684-4-0"
+           inkscape:connector-curvature="0" />
+      </g>
       <g
          id="g5553"
          transform="translate(-9.375,0.84066785)"
@@ -2918,7 +2958,7 @@
            style="color:#000000;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:medium;line-height:normal;font-family:sans-serif;font-variant-ligatures:normal;font-variant-position:normal;font-variant-caps:normal;font-variant-numeric:normal;font-variant-alternates:normal;font-feature-settings:normal;text-indent:0;text-align:start;text-decoration:none;text-decoration-line:none;text-decoration-style:solid;text-decoration-color:#000000;letter-spacing:normal;word-spacing:normal;text-transform:none;writing-mode:lr-tb;direction:ltr;text-orientation:mixed;dominant-baseline:auto;baseline-shift:baseline;text-anchor:start;white-space:normal;shape-padding:0;clip-rule:nonzero;display:inline;overflow:visible;visibility:visible;opacity:1;isolation:auto;mix-blend-mode:normal;color-interpolation:sRGB;color-interpolation-filters:linearRGB;solid-color:#000000;solid-opacity:1;vector-effect:none;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:4.00004;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-dashoffset:0;stroke-opacity:1;color-rendering:auto;image-rendering:auto;shape-rendering:auto;text-rendering:auto;enable-background:accumulate" />
       </g>
       <g
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+         transform="translate(204.3142,1.1058258)"
          style="display:inline;stroke-width:1.00002"
          id="g5553-4-4">
         <path
@@ -2994,7 +3034,7 @@
       </g>
       <g
          style="display:inline;stroke-width:1.00002"
-         transform="translate(441.84135,6.6888529)"
+         transform="translate(462.46635,6.6888529)"
          id="g9098">
         <path
            inkscape:connector-curvature="0"
diff --git a/subworkflows/local/germline_variant_calling.nf b/subworkflows/local/germline_variant_calling.nf
index ee0d45c726..9096f68b86 100644
--- a/subworkflows/local/germline_variant_calling.nf
+++ b/subworkflows/local/germline_variant_calling.nf
@@ -18,14 +18,14 @@ workflow GERMLINE_VARIANT_CALLING {
         bwa                           // channel: [mandatory] bwa
         dbsnp                         // channel: [mandatory] dbsnp
         dbsnp_tbi                     // channel: [mandatory] dbsnp_tbi
-        known_sites
-        known_sites_tbi
         dict                          // channel: [mandatory] dict
         fasta                         // channel: [mandatory] fasta
         fasta_fai                     // channel: [mandatory] fasta_fai
         intervals                     // channel: [mandatory] intervals/target regions
         intervals_bed_gz_tbi          // channel: [mandatory] intervals/target regions index zipped and indexed
         intervals_bed_combined        // channel: [mandatory] intervals/target regions in one file unzipped
+        known_sites
+        known_sites_tbi
         // joint_germline                // val: true/false on whether to run joint_germline calling, only works in combination with haplotypecaller at the moment
 
     main:
diff --git a/subworkflows/local/tumor_variant_calling.nf b/subworkflows/local/tumor_variant_calling.nf
index 0d12f83702..f9c8b44f07 100644
--- a/subworkflows/local/tumor_variant_calling.nf
+++ b/subworkflows/local/tumor_variant_calling.nf
@@ -10,25 +10,27 @@ include { RUN_STRELKA_SINGLE                      } from '../nf-core/variantcall
 include { RUN_CONTROLFREEC_TUMORONLY              } from '../nf-core/variantcalling/controlfreec/tumoronly/main.nf'
 include { RUN_CNVKIT_TUMORONLY                    } from '../nf-core/variantcalling/cnvkit/tumoronly/main.nf'
 include { RUN_MPILEUP                             } from '../nf-core/variantcalling/mpileup/main'
+include { RUN_TIDDIT                              } from '../nf-core/variantcalling/tiddit/main.nf'
 
 workflow TUMOR_ONLY_VARIANT_CALLING {
     take:
         tools                         // Mandatory, list of tools to apply
         cram_recalibrated             // channel: [mandatory] cram
+        bwa                           // channel: [optional] bwa
+        chr_files
         dbsnp                         // channel: [mandatory] dbsnp
         dbsnp_tbi                     // channel: [mandatory] dbsnp_tbi
         dict                          // channel: [mandatory] dict
         fasta                         // channel: [mandatory] fasta
         fasta_fai                     // channel: [mandatory] fasta_fai
+        germline_resource             // channel: [optional]  germline_resource
+        germline_resource_tbi         // channel: [optional]  germline_resource_tbi
         intervals                     // channel: [mandatory] intervals/target regions
         intervals_bed_gz_tbi          // channel: [mandatory] intervals/target regions index zipped and indexed
         intervals_bed_combined        // channel: [mandatory] intervals/target regions in one file unzipped
-        germline_resource             // channel: [optional]  germline_resource
-        germline_resource_tbi         // channel: [optional]  germline_resource_tbi
+        mappability
         panel_of_normals              // channel: [optional]  panel_of_normals
         panel_of_normals_tbi          // channel: [optional]  panel_of_normals_tbi
-        chr_files
-        mappability
 
     main:
 
@@ -39,6 +41,7 @@ workflow TUMOR_ONLY_VARIANT_CALLING {
     manta_vcf           = Channel.empty()
     mutect2_vcf         = Channel.empty()
     strelka_vcf         = Channel.empty()
+    tiddit_vcf          = Channel.empty()
 
     // Remap channel with intervals
     cram_recalibrated_intervals = cram_recalibrated.combine(intervals)
@@ -152,16 +155,23 @@ workflow TUMOR_ONLY_VARIANT_CALLING {
         ch_versions = ch_versions.mix(RUN_STRELKA_SINGLE.out.versions)
     }
 
+        //TIDDIT
+    if (tools.contains('tiddit')){
+        RUN_TIDDIT(cram_recalibrated,
+                fasta,
+                bwa)
 
-    // if (tools.contains('tiddit')){
-    // }
+        tiddit_vcf = RUN_TIDDIT.out.tiddit_vcf
+        ch_versions = ch_versions.mix(RUN_TIDDIT.out.versions)
+    }
 
-    emit:
-    versions = ch_versions
 
+    emit:
     freebayes_vcf
     manta_vcf
     mutect2_vcf
     strelka_vcf
+    tiddit_vcf
 
+    versions = ch_versions
 }
diff --git a/tests/test_tools.yml b/tests/test_tools.yml
index 834f3b622b..d1a12b4c8f 100644
--- a/tests/test_tools.yml
+++ b/tests/test_tools.yml
@@ -81,6 +81,17 @@
     - path: results/variant_calling/sample1/tiddit/sample1.vcf.gz
     - path: results/variant_calling/sample1/tiddit/sample1.vcf.gz.tbi
 
+- name: Run variant calling on tumor_only sample with tiddit
+  command: nextflow run main.nf -profile test,tools_tumoronly,docker --tools tiddit -c ./tests/nextflow.config
+  tags:
+    - tiddit
+    - tumor_only
+    - variant_calling
+  files:
+    - path: results/variant_calling/sample2/tiddit/sample2.ploidies.tab
+    - path: results/variant_calling/sample2/tiddit/sample2.vcf.gz
+    - path: results/variant_calling/sample2/tiddit/sample2.vcf.gz.tbi
+
 - name: Run variant calling on somatic samples with controlfreec
   command: nextflow run main.nf -profile test,tools_somatic,docker --tools controlfreec -c ./tests/nextflow.config
   tags:
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index 9e2c3b3777..28750c994f 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -792,34 +792,35 @@ workflow SAREK {
             [],
             dbsnp,
             dbsnp_tbi,
-            known_sites,
-            known_sites_tbi,
             dict,
             fasta,
             fasta_fai,
             intervals,
             intervals_bed_gz_tbi,
-            intervals_bed_combined)
+            intervals_bed_combined,
+            known_sites,
+            known_sites_tbi)
             // params.joint_germline)
 
         // TUMOR ONLY VARIANT CALLING
         TUMOR_ONLY_VARIANT_CALLING(
             params.tools,
             cram_variant_calling_tumor_only,
+            [],
+            chr_files,
             dbsnp,
             dbsnp_tbi,
             dict,
             fasta,
             fasta_fai,
+            germline_resource,
+            germline_resource_tbi,
             intervals,
             intervals_bed_gz_tbi,
             intervals_bed_combined,
-            germline_resource,
-            germline_resource_tbi,
+            mappability,
             pon,
-            pon_tbi,
-            chr_files,
-            mappability
+            pon_tbi
         )
 
         // PAIR VARIANT CALLING
@@ -855,6 +856,7 @@ workflow SAREK {
         vcf_to_annotate = vcf_to_annotate.mix(TUMOR_ONLY_VARIANT_CALLING.out.mutect2_vcf)
         vcf_to_annotate = vcf_to_annotate.mix(TUMOR_ONLY_VARIANT_CALLING.out.manta_vcf)
         vcf_to_annotate = vcf_to_annotate.mix(TUMOR_ONLY_VARIANT_CALLING.out.strelka_vcf)
+        vcf_to_annotate = vcf_to_annotate.mix(TUMOR_ONLY_VARIANT_CALLING.out.tiddit_vcf)
         vcf_to_annotate = vcf_to_annotate.mix(PAIR_VARIANT_CALLING.out.mutect2_vcf)
         vcf_to_annotate = vcf_to_annotate.mix(PAIR_VARIANT_CALLING.out.manta_vcf)
         vcf_to_annotate = vcf_to_annotate.mix(PAIR_VARIANT_CALLING.out.strelka_vcf)