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mirdeep2 output missing #330

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LucasMcNU opened this issue Mar 23, 2024 · 2 comments
Closed

mirdeep2 output missing #330

LucasMcNU opened this issue Mar 23, 2024 · 2 comments
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@LucasMcNU
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Description of the bug

Running the smRNAseq pipeline does not produce the output listed here. MirDeep2 output is missing. Everything else is there. Running this on the Northwestern University HPC using their configuration file. Used the following genome file:

projects/genomicsshare/AWS_iGenomes/references/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa

Command I used is below:

Command used and terminal output

## Load modules
module purge

module load nextflow/23.04.3
module load singularity/latest
module load graphviz/2.40.1
module load java/jdk11.0.10

project_dir=/projects/b1042/BackmanLab/Lucas/101823_rnaseq/Backman19_10.18.2023/smallRNA/
cd $project_dir
OUTDIR=${project_dir}nextflow_results/
[ -d $OUTDIR ] || mkdir -p $OUTDIR

nextflow run nf-core/smrnaseq \
-profile nu_genomics \
--input $project_dir/samplesheet.csv \
--genome 'GRCh38' \
--mirtrace_species 'hsa' \
--protocol 'nextflex' \
--outdir $OUTDIR

Relevant files

nextflow.log

System information

No response

@LucasMcNU LucasMcNU added the bug Something isn't working label Mar 23, 2024
@mazzalab
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same here

@apeltzer apeltzer added this to the 2.3.1 milestone Mar 25, 2024
@apeltzer
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#343 fixed

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