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Enabling the contaminant filter the pipeline fails when pulling the blat docker container quay.io/biocontainers/blat:36--0.
The container image seems to be broken: docker run quay.io/biocontainers/blat:36--0 returns the error.
Command used and terminal output
nextflow run nf-core/smrnaseq \ -profile docker \ -resume \ --input 'samplesheet.csv' \ --genome 'GRCh38' \ --mirtrace_species 'hsa' \ --protocol 'illumina' \ --outdir 'Results' \ --fastp_max_length 35 \ --filter_contamination true \ --rrna 'human_rRNAs.fasta' \ --trna 'human_tRNAs.fasta' \ --pirna 'hsa_pirna.fa' \ --other_contamination 'PhiX_and_adapter.fa' \ --save_trimmed_fail true \ --save_aligned true \ --save_aligned_mirna_quant true \ --save_merged true \ --three_prime_adapter 'auto-detect'ERROR ~ Error executing process > 'NFCORE_SMRNASEQ:CONTAMINANT_FILTER:BLAT_PIRNA (null)'Caused by: Process `NFCORE_SMRNASEQ:CONTAMINANT_FILTER:BLAT_PIRNA (null)` terminated with an error exit status (125)Command executed: echo other blat -out=blast8 hairpin.fa hsa_pirna.fa /dev/stdout | awk 'BEGIN{FS=" "}{if($11 < 1e-5)print $1;}' | uniq > mirnahit.txt awk 'BEGIN { while((getline<"mirnahit.txt")>0) l[">"$1]=1 } /^>/ {x = l[$1]} {if(!x) print }' hsa_pirna.fa > filtered.fa cat <<-END_VERSIONS > versions.yml "NFCORE_SMRNASEQ:CONTAMINANT_FILTER:BLAT_PIRNA": blat: $(echo $(blat) | grep Standalone | awk '{ if (match($0,/[0-9]*[0-9]/,m)) print m[0] }') END_VERSIONSCommand exit status: 125Command output: (empty)Command error: Unable to find image 'quay.io/biocontainers/blat:36--0' locally 36--0: Pulling from biocontainers/blat docker: [DEPRECATION NOTICE] Docker Image Format v1 and Docker Image manifest version 2, schema 1 support is disabled by default and will be removed in an upcoming release. Suggest the author of quay.io/biocontainers/blat:36--0 to upgrade the image to the OCI Format or Docker Image manifest v2, schema 2. More information at https://docs.docker.com/go/deprecated-image-specs/. See 'docker run --help'.Work dir: /sharedfs01/enrico/Joost/AGO2_circrnas/smrnaseq/work/d2/91d148e1932090db23675601dc03feTip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line -- Check '.nextflow.log' file for details
Relevant files
No response
System information
N E X T F L O W ~ version 23.04.1
Hardware HPC
Executor SLURM
Container engine: Docker
OS: Ubuntu 22.04 LTS
nf-core/smrnaseq v2.3.1-g5901bea
The text was updated successfully, but these errors were encountered:
Maybe it would be worth to move the BLAT containers to nf-core/modules instead, importing them from there and making sure that they are already updated / matching the latest standards to improve quality.
Description of the bug
Enabling the contaminant filter the pipeline fails when pulling the blat docker container quay.io/biocontainers/blat:36--0.
The container image seems to be broken:
docker run quay.io/biocontainers/blat:36--0
returns the error.Command used and terminal output
Relevant files
No response
System information
N E X T F L O W ~ version 23.04.1
Hardware HPC
Executor SLURM
Container engine: Docker
OS: Ubuntu 22.04 LTS
nf-core/smrnaseq v2.3.1-g5901bea
The text was updated successfully, but these errors were encountered: