diff --git a/CHANGELOG.md b/CHANGELOG.md
index d3c0792519..d64c03267e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -7,6 +7,7 @@
 ### Linting
 
 - Warn if container access is denied ([#2270](https://github.com/nf-core/tools/pull/2270))
+- Error if module container specification has quay.io as prefix when it shouldn't have.
 
 ### Modules
 
diff --git a/nf_core/modules/lint/main_nf.py b/nf_core/modules/lint/main_nf.py
index 102d5ead12..bfdb92c5cf 100644
--- a/nf_core/modules/lint/main_nf.py
+++ b/nf_core/modules/lint/main_nf.py
@@ -290,6 +290,14 @@ def check_process_section(self, lines, fix_version, progress_bar):
             else:
                 self.failed.append(("docker_tag", "Unable to parse docker tag", self.main_nf))
                 docker_tag = None
+            if l.startswith("quay.io/biocontainers") and not l.startswith("quay.io/biocontainers/mulled"):
+                self.failed.append(
+                    (
+                        "container_links",
+                        "quay.io/biocontainers prefix found, please use biocontainers instead",
+                        self.main_nf,
+                    )
+                )
             if l.startswith("biocontainers/"):
                 # When we think it is a biocontainer, assume we are querying quay.io/biocontainers and insert quay.io as prefix
                 l = "quay.io/" + l